Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F50 I1 R1
|
163 |
55.0 |
1534755 |
98.6% |
1513268 |
150.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
3,028,476 |
T→C |
G452G (GGT→GGC) |
ghxQ → |
guanine/hypoxanthine permease, high affinity; guanine/hypoxanthine:H+ symporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 3,028,476 | 0 | T | C | 100.0%
| 176.3
/ NA
| 46 | G452G (GGT→GGC) | ghxQ | guanine/hypoxanthine permease, high affinity; guanine/hypoxanthine:H+ symporter |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (29/17); total (29/17) |
TCATCGTTCTGACTTGTAATATCGTTACCGGTATTATGCTGGGCTTTGTGACACTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGTTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTCAGTAAGCAACTGCAGCGCCTGTGTTGGGCAAGATT > NC_000913/3028328‑3028621
|
tCATCGTTCTGACTTGTAATATCGTTACCGGTATTATGCTGGGCTTTGTGACACTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTg > 1:407532/1‑151 (MQ=255)
cATCGTTCTGACTTGTAATATCGTTACCGGTATTATGCTGGGCTTTGTGACACTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTgg > 1:519361/1‑151 (MQ=255)
cGTTCTGACTTGTAATATCGTTACCGGTATTATGCTGGGCTTTGTGACACTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCa < 1:341846/151‑1 (MQ=255)
cGTTCTGACTTGTAATATCGTTACCGGTATTATGCTGGGCTTTGTGACACTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCa < 2:395385/151‑1 (MQ=255)
aaTATCGTTACCGGTATTATGCTGGGCTTTGTGACACTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGAtt < 2:226362/150‑1 (MQ=255)
atatCGTTACCGGTATTATGCTGGGCTTTGTGACACTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATtc > 2:410790/1‑150 (MQ=255)
atCGTTACCGGTATTATGCTGGGCTTTGTGACACTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATtctc < 1:481700/150‑1 (MQ=255)
cGTTACCGGTATTATGCTGGGCTTTGTGACACTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCcgc > 2:495564/1‑151 (MQ=255)
gTTACCGGTATTATGCTGGGCTTTGTGACACTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCcgcg < 1:510943/151‑1 (MQ=255)
ttACCGGTATTATGCTGGGCTTTGTGACACTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCgcgc > 1:688736/1‑151 (MQ=255)
aCCGGTATTATGCTGGGCTTTGTGACACTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCt > 1:724572/1‑151 (MQ=255)
gTATTATGCTGGGCTTTGTGACACTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTc > 1:148657/1‑150 (MQ=255)
tGCTGGGCTTTGTGACACTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCgg > 1:127906/1‑151 (MQ=255)
gggCTTTGTGACACTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCgg > 2:114259/1‑147 (MQ=255)
tgtgACACTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGAtttcttt > 2:571570/1‑151 (MQ=255)
gtgACACTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGAtttcttt < 1:422751/150‑1 (MQ=255)
cacTCGCCGTAGGCCGCGTCTTTGCACGCGAATGGCAAACGCTGAATATTGGCACGGCGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGCGGCTGGGCAATCTCATAGATTCTCCGCGCCTTTCAGCGCGCAGATTTCTTTCag > 2:346376/1‑149 (MQ=255)
acTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCag > 2:699649/1‑148 (MQ=255)
tGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAgg > 2:83882/1‑151 (MQ=255)
aGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATtctc > 2:197259/1‑150 (MQ=255)
aGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCa < 1:631693/151‑1 (MQ=255)
cgcgTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGc < 2:175726/151‑1 (MQ=255)
tGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGcaac > 1:607452/1‑150 (MQ=255)
gCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGcaaca > 2:645277/1‑150 (MQ=255)
gCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGcaac > 2:172950/1‑149 (MQ=255)
aCGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATa < 2:466906/151‑1 (MQ=255)
gcgAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACg > 1:543352/1‑151 (MQ=255)
gAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACgct > 1:203135/1‑151 (MQ=255)
aTGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACgctgc > 2:67262/1‑151 (MQ=255)
aaaGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACGCTGCTGGCgg > 1:603877/1‑151 (MQ=255)
aaaGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACGCTGCTGGCgg > 2:172459/1‑151 (MQ=255)
aaaGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACGCTGCTGGCgg > 2:228741/1‑151 (MQ=255)
gCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACGCTGCTGGCGGACt > 1:108927/1‑151 (MQ=255)
gAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACGCTGCTGGCGGACTc < 1:243518/149‑1 (MQ=255)
gAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACGCTGCTGGCGGACTCGc > 2:252729/1‑151 (MQ=255)
atatTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCCCCGCTGGCAACAATACGCTGCTGGCGGACTCGCCt < 1:269239/151‑1 (MQ=255)
atTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACGCTGCTGGCGGACTCGCCTg > 2:608151/1‑150 (MQ=255)
gATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTc < 1:541632/150‑1 (MQ=255)
ttACTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTCAGTa > 1:547945/1‑149 (MQ=255)
aCTGCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTCAGTAAGCa < 2:325320/151‑1 (MQ=255)
gCCGCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTCAGTAAGCAACt < 2:519337/151‑1 (MQ=255)
gCACTGGTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTCAGTAAGCAACTGCa < 1:402339/151‑1 (MQ=255)
ggTCGCATTTTACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTCAGTAAGCAACTGCAGCGcc > 2:174282/1‑151 (MQ=255)
ttACGCGGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTCAGTAAGCAACTGCAGCGCCTGTGTTgg < 2:531510/150‑1 (MQ=255)
cgcgGGTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTCAGTAAGCAACTGCAGCGCCTGTGTTGGGCa > 1:270538/1‑150 (MQ=255)
gggTGGCTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTCAGTAAGCAACTGCAGCGCCTGTGTTGGGCAAGAt < 1:723466/151‑1 (MQ=255)
ggtggcTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTCAGTAAGCAACTGCAGCGCCTGTGTTGGGCAAGAtt < 2:543324/151‑1 (MQ=255)
ggtggcTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTCAGTAAGCAACTGCAGCGCCTGTGTTGGGCAAGAtt > 2:600980/1‑151 (MQ=255)
|
TCATCGTTCTGACTTGTAATATCGTTACCGGTATTATGCTGGGCTTTGTGACACTGGTCGTAGGCCGCGTCTTTGCACGCGAATGGCAAAAGCTGAATATTGGTACGGTGATCATTACTGCCGCACTGGTCGCATTTTACGCGGGTGGTTGGGCAATCTAATAGATTCTCCGCGCCTTTCAGCGCGGAGATTTCTTTCAGAGAGGATTCTCACCGCTGGCAACAATACGCTGCTGGCGGACTCGCCTGAGTTCTCTTTCAGTAAGCAACTGCAGCGCCTGTGTTGGGCAAGATT > NC_000913/3028328‑3028621
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
