Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F50 I1 R1	163	55.0	1534755	98.6%	1513268	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,030,937	T→C	intergenic (‑6/‑430)	ygfT ← / → uacT	putative oxidoreductase, Fe‑S subunit/nucleotide‑binding subunit/uric acid permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,030,937	0	T	C	100.0%	125.3 / NA	36	intergenic (‑6/‑430)	ygfT/uacT	putative oxidoreductase, Fe‑S subunit/nucleotide‑binding subunit/uric acid permease
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (5/31); total (5/31)

CCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCATCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCAATAAACGGGCATATATTAGGTAGCATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACA  >  NC_000913/3030789‑3031083

ccTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAACCTTATTCATTTGCAccc                                                                                                                                                  <  2:34079/151‑1 (MQ=255)

   ttCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCAcccc                                                                                                                                                 <  2:415929/149‑1 (MQ=255)

   ttCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCt                                                                                                                                                <  1:510998/150‑1 (MQ=255)

    tCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCtt                                                                                                                                               <  2:707330/150‑1 (MQ=255)

    tCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCtt                                                                                                                                               <  1:693430/150‑1 (MQ=255)

     cccTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTc                                                                                                                                             <  2:368633/151‑1 (MQ=255)

         aCAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACACCTTTCAttt                                                                                                                                         <  2:726833/151‑1 (MQ=255)

              gTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGa                                                                                                                                    <  1:396451/151‑1 (MQ=255)

                ggATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGt                                                                                                                                  <  2:536030/151‑1 (MQ=255)

                     cgcgGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGt                                                                                                                             <  1:423872/151‑1 (MQ=255)

                           cGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCACAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGtcttct                                                                                                                        <  1:463025/150‑1 (MQ=255)

                                gTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAAtt                                                                                                                  <  1:211194/151‑1 (MQ=255)

                                  cACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAGGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAAttt                                                                                                                 <  2:315557/150‑1 (MQ=255)

                                      gtgACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTcgcg                                                                                                             <  1:621927/150‑1 (MQ=255)

                                      gtgACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTcgc                                                                                                              <  1:223350/149‑1 (MQ=255)

                                          cTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGc                                                                                                         >  1:437827/1‑150 (MQ=255)

                                            cAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCa                                                                                                        <  1:653373/149‑1 (MQ=255)

                                             aGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCAATa                                                                                                     <  1:61467/151‑1 (MQ=255)

                                              gCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCAATaa                                                                                                    <  1:599366/151‑1 (MQ=255)

                                                 gCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCAATAAACg                                                                                                 <  1:674943/151‑1 (MQ=255)

                                                    aGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATTCATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCAATAAACGGGc                                                                                              <  1:27257/151‑1 (MQ=255)

                                                            tGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCAATAAACGGGCatatat                                                                                        <  2:60964/149‑1 (MQ=255)

                                                                 gtgtgCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCAATAAACGGGCATATATTAGGTAg                                                                                 <  2:307994/151‑1 (MQ=255)

                                                                  tgtgCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCAATAAACGGGCATATATTAGGTAGc                                                                                >  2:698714/1‑151 (MQ=255)

                                                                     gCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCAATAAACGGGCATATATTAGGTAGCATg                                                                             <  2:529974/151‑1 (MQ=255)

                                                                              caGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCAATAAACGGGCATATATTAGGTAGCATGACCGtttt                                                                     <  2:437806/150‑1 (MQ=255)

                                                                              caGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCAATAAACGGGCATATATTAGGTAGCATGACCGTTTTa                                                                    <  2:484501/151‑1 (MQ=255)

                                                                                ggCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCAATAAACGGGCATATATTAGGTAGCATGACCGTTTTAAt                                                                  <  2:541260/151‑1 (MQ=255)

                                                                                   aaTTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCAATAAACGGGCATATATTAGGTAGCATGACCGTTTTAATtat                                                               <  2:305097/151‑1 (MQ=255)

                                                                                   aaTTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCAATAAACGGGCATATATTAGGTAGCATGACCGTTTTAATtat                                                               <  2:220130/151‑1 (MQ=255)

                                                                                               aTGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCAATAAACGGGCATATATTAGGTAGCATGACCGTTTTAATTATAATTTTGCGACt                                                   >  1:167182/1‑151 (MQ=255)

                                                                                               aTGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCAATAAACGGGCATATATTAGGTAGCATGACCGTTTTAATTATAATTTTGCGACt                                                   <  2:556503/151‑1 (MQ=255)

                                                                                                      ccTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCAATAAACGGGCATATATTAGGTAGCATGACCGTTTTAATTATAATTTTGCGACTGAGTTCa                                            <  1:393855/151‑1 (MQ=255)

                                                                                                           aCATTCCGCAGCTTCCTGAACAATAATCTAATTCATTTGTACCCCTCACATTTAATGAATTACGTCTTCCCTAATTTCTCGGTGCAATAATCGGGCATATATTAGGTAGCATGTGCGGATTAAATATAATTTTGCGACGGAgaacaagt                                         <  2:441855/149‑8 (MQ=255)

                                                                                                                        tCAGCAGCAATAAACTTATTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCAATAAACGGGCATATATTAGGTAGCATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATc                          >  1:56107/1‑151 (MQ=255)

                                                                                                                                         aTTCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCAATAAACGGGCATATATTAGGTAGCATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCatta          <  2:120640/150‑1 (MQ=255)

                                                                                                                                           tCATTTGCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCAATAAACGGGCATATATTAGGTAGCATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTAttt       >  2:202888/1‑151 (MQ=255)

                                                                                                                                                 gCACCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCAATAAACGGGCATATATTAGGTAGCATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACa  <  2:646619/150‑1 (MQ=255)

CCTTTCCCTACAACGTGGATACGCGGTCGAAAGTCACTGTGACTCAGCGGCCAGTTTTCTTGATTGTGTGCCACCGCACAGGCAATTTCACAAGCATGACAGCCTATACATTCCGCAGCTTCAGCAGCAATAAACTTATTCATTTGCATCCCTTTCATTTAATGAGTTATGTCTTCTCAAATTTCGCGATGCAATAAACGGGCATATATTAGGTAGCATGACCGTTTTAATTATAATTTTGCGACTGAGTTCAAATTATTGCCCCTCAATCTATGCCGCTTTCATTATTTCAACA  >  NC_000913/3030789‑3031083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A