Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F50 I1 R1 163 55.0 1534755 98.6% 1513268 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,280,131 A→G E406E (GAA→GAG)  kbaZ → tagatose 6‑phosphate aldolase 1, kbaZ subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,280,1310AG100.0% 199.7 / NA 54E406E (GAA→GAG) kbaZtagatose 6‑phosphate aldolase 1, kbaZ subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/27);  total (27/27)

ACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAACTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTC  >  NC_000913/3279983‑3280277
                                                                                                                                                    |                                                                                                                                                  
aCTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATGCCGCTGCGGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCt                                                                                                                                                  <  2:409141/151‑1 (MQ=255)
 cTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTc                                                                                                                                                 >  2:60853/1‑151 (MQ=255)
     ccGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCAtt                                                                                                                                              >  2:515655/1‑150 (MQ=255)
     ccGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTa                                                                                                                                             <  1:37108/151‑1 (MQ=255)
      cGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTAt                                                                                                                                            >  1:443521/1‑151 (MQ=255)
          cAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAAc                                                                                                                                        >  1:704082/1‑151 (MQ=255)
             ccAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCat                                                                                                                                     >  2:344440/1‑151 (MQ=255)
              cAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCata                                                                                                                                    <  1:724585/151‑1 (MQ=255)
                gATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATAtt                                                                                                                                  <  2:28282/151‑1 (MQ=255)
                gATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATAtt                                                                                                                                  <  2:367426/151‑1 (MQ=255)
                gATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATAtt                                                                                                                                  <  1:763346/151‑1 (MQ=255)
                   tgatgaCGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCa                                                                                                                                <  1:140341/150‑1 (MQ=255)
                           cTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATcc                                                                                                                        >  2:587583/1‑150 (MQ=255)
                             ttCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCt                                                                                                                       <  1:438886/149‑1 (MQ=255)
                                           aCGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACcacaca                                                                                                       <  2:292457/151‑1 (MQ=255)
                                            cGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACcacac                                                                                                        >  1:719224/1‑149 (MQ=255)
                                             gTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGCCATCCTGGCGCAGTACCACACAGc                                                                                                     >  1:592265/1‑151 (MQ=255)
                                             gTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGc                                                                                                     >  2:688732/1‑151 (MQ=255)
                                                 tCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCtgt                                                                                                 >  1:625534/1‑151 (MQ=255)
                                                  cTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCtgtg                                                                                                >  1:170043/1‑151 (MQ=255)
                                                    ggCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCtgt                                                                                                 <  2:242653/148‑1 (MQ=255)
                                                     gCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAg                                                                                             <  2:97353/151‑1 (MQ=255)
                                                               cAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGc                                                                                   <  2:548380/151‑1 (MQ=255)
                                                                aaTTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCa                                                                                  <  2:26488/151‑1 (MQ=255)
                                                                    ccgctgccgctgATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATGAGCAAAAc                                                                              <  1:644688/151‑1 (MQ=255)
                                                                    ccgctgccgctgATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCaaaa                                                                               <  1:378906/150‑1 (MQ=255)
                                                                     cgctgccgctgATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAAc                                                                              >  1:49125/1‑150 (MQ=255)
                                                                     cgctgccgctgATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAAc                                                                              >  1:340412/1‑150 (MQ=255)
                                                                            gctgATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAgg                                                                       >  2:307849/1‑150 (MQ=255)
                                                                            gctgATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGa                                                                      <  2:308213/151‑1 (MQ=255)
                                                                                 tCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACg                                                                 >  1:115808/1‑151 (MQ=255)
                                                                                      cAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATg                                                            <  2:98469/151‑1 (MQ=255)
                                                                                        gTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGcc                                                          <  1:619389/151‑1 (MQ=255)
                                                                                                cGCTGCAGTACGTGAAAGTTAGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAAGACATCCTGGCGCAGGACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAata                                                     >  2:83115/1‑148 (MQ=255)
                                                                                                    gCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGtttt                                              >  2:641675/1‑151 (MQ=255)
                                                                                                    gCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGttt                                               <  2:761485/150‑1 (MQ=255)
                                                                                                    gCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGtt                                                <  2:707434/149‑1 (MQ=255)
                                                                                                          cGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCg                                        <  2:374324/151‑1 (MQ=255)
                                                                                                          cGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCg                                        <  1:630311/151‑1 (MQ=255)
                                                                                                             gAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGAt                                     >  1:618648/1‑151 (MQ=255)
                                                                                                               aaGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATtg                                   >  1:475995/1‑151 (MQ=255)
                                                                                                                aGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATtga                                  <  2:34812/151‑1 (MQ=255)
                                                                                                                aGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATtga                                  <  1:421542/151‑1 (MQ=255)
                                                                                                                       tCCGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGc                           >  2:613574/1‑151 (MQ=255)
                                                                                                                        ccGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGc                           <  2:208093/150‑1 (MQ=255)
                                                                                                                        ccGGCGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCt                          <  2:90475/151‑1 (MQ=255)
                                                                                                                            cGAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGAt                      <  1:196287/151‑1 (MQ=255)
                                                                                                                             gAGCTGCAGCCAACGCCAGGGGCGCTCATTATCAACCATATTCAGGACCTCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAACGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAAAgcttgctt                     >  1:520451/1‑151 (MQ=255)
                                                                                                                             gAGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCGAATATTGTTTTAAGCCGGATTGATGAACGCTTGAtt                     >  1:80577/1‑151 (MQ=255)
                                                                                                                              aGCTGCAGCCAACGCCACTGGAGCTCATTAGCAACCATATTCAGGACATCCAGGCGCAGTACCACGCAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGAtt                     >  2:118952/1‑150 (MQ=255)
                                                                                                                              aGCTGCAGCCAACGCCACGGGAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTc                    >  1:417725/1‑151 (MQ=255)
                                                                                                                                                gggAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTc  >  2:64144/1‑151 (MQ=255)
                                                                                                                                                gggAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTc  >  2:328609/1‑151 (MQ=255)
                                                                                                                                                 ggAGCTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTc  >  1:87744/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
ACTGGCCGGACAGCCAGATTGATGACGCTTTCGCTCATCTGGTACGTAATCTGGCGGATTCACCAATTCCGCTGCCGCTGATCAGCCAGTATCTGCCGCTGCAGTACGTGAAAGTTCGCTCCGGCGAGCTGCAGCCAACGCCACGGGAACTCATTATCAACCATATTCAGGACATCCTGGCGCAGTACCACACAGCCTGTGAAGGCCAATAAGCAAAACAAAGAGGAACACGCTATGCCAAATATTGTTTTAAGCCGGATTGATGAACGCTTGATTCACGGTCAGGTCGGCGTTC  >  NC_000913/3279983‑3280277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A