Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F50 I1 R1	163	55.0	1534755	98.6%	1513268	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,372,102	A→G	V158A (GTC→GCC)	nanT ←	sialic acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,372,102	0	A	G	100.0%	123.6 / NA	39	V158A (GTC→GCC)	nanT	sialic acid transporter
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (19/20); total (19/20)

TTACCTGCGTGTTTCTCTTTCCAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGT  >  NC_000913/3371955‑3372247

ttACCTGCGTGTTTCTCTTTCCAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAacggcg                                                                                                                                                 <  1:434469/150‑1 (MQ=255)

 tACCTGCGTGTTTCTCTTTCCAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACggcggc                                                                                                                                               >  2:6074/1‑151 (MQ=255)

                ctTTCCAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAg                                                                                                                                  <  2:129218/149‑1 (MQ=255)

                ctTTCCACTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGTTAAGATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCACAAGACCGGAAACACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGACaaac                                                                                                                                 >  2:487011/1‑148 (MQ=255)

                            ccgcttccgGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATTGGAAAAATGCCGATAAAGAACAGCGCACGACAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCa                                                                                                                        <  2:244852/147‑1 (MQ=255)

                                   cgGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTg                                                                                                             <  2:481511/151‑1 (MQ=255)

                                   cgGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTg                                                                                                             >  2:370512/1‑151 (MQ=255)

                                     ggATGTTTTTACGCAGCCAGAGAGCAAAGATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGc                                                                                                           >  1:756636/1‑151 (MQ=255)

                                       aTGTTTTTACTCAGCCAGAGAGCAAAGATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCtt                                                                                                         <  2:713832/151‑1 (MQ=255)

                                        tGTTTTTACGCAGCCCGAGAGCAAAGATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGAGACGGCGGCCCCCACCGAGAAGCCTGAAATCAAAAAACCACTGGCt                                                                                                          <  2:523320/149‑1 (MQ=255)

                                            tttACGCAGCCAGAGAGCAAAGATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGtt                                                                                                     >  2:649041/1‑150 (MQ=255)

                                                cGCAGCCAGAGAGCAAAGATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCCCCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCa                                                                                                <  1:314292/151‑1 (MQ=255)

                                                 gCAGCCAGAGAGCAAAGATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGc                                                                                                 >  1:428505/1‑149 (MQ=255)

                                                 gCAGCCAGAGAGCAAAGATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGc                                                                                                 >  2:144228/1‑149 (MQ=255)

                                                   aGCCAGAGAGCAAAGATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGaa                                                                                                                            <  1:151528/120‑1 (MQ=255)

                                                   aGCCAGAGAGCAAAGATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGaa                                                                                                                            >  2:151521/1‑120 (MQ=255)

                                                     ccAGAGAGCAAAGATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGt                                                                                           >  1:647308/1‑151 (MQ=255)

                                                         atagCAAAGATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGGCCTGAGCGGAAACTGCAGCCCCCACAGAGAAGCCTGCAATCAAAAAACCGCTGGCTTTGTTACGCGGATGTTTTg                                                                                       <  2:478327/149‑1 (MQ=255)

                                                         agagCAAAGATGATTGGCAAAATGCCGATAAAGGACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTg                                                                                       >  2:422152/1‑151 (MQ=255)

                                                         agagCAAAGATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTg                                                                                       <  1:273247/151‑1 (MQ=255)

                                                            gCAAAGATGATTGCCAAAATGCCAATAACGAACAGCGCACGACAGACCCAGACCGGAGCCACCATGCTATAGACCTGAGCGGCAACGGCGGCTCCCTCAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTggac                                                                                    <  2:96603/151‑3 (MQ=255)

                                                               aaGATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGc                                                                                 >  1:537387/1‑151 (MQ=255)

                                                                        ggCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATg                                                                         <  2:94491/150‑1 (MQ=255)

                                                                           aaaaTGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACAt                                                                     >  1:108544/1‑151 (MQ=255)

                                                                           aaaaTGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACAt                                                                     <  2:482566/151‑1 (MQ=255)

                                                                                    aTAACGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGTCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGCTGTTTTGGCCAGCTTTCAATGACATAGGTGgcgc                                                            >  2:327787/1‑151 (MQ=255)

                                                                                     tAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCt                                                           >  2:483736/1‑151 (MQ=255)

                                                                                        aGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTgg                                                         >  1:390552/1‑150 (MQ=255)

                                                                                         gAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGa                                                        >  1:98500/1‑150 (MQ=255)

                                                                                          aaCAGCGCACGCCAGCCCCTGACCTGATCCACCAGGCTATAGACATGAGCGGCAACGGCGGCCCCCACATTGAAGCCTGTAATCAAAAAACCACTGGCTTTGTTACGCAGAGGTTTTGTCCAGCTTTCAATGACATAGGTGGCGCTGGaa                                                       <  2:230642/150‑1 (MQ=255)

                                                                                                     ccAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCAccc                                           >  1:521922/1‑151 (MQ=255)

                                                                                                               aCCGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCa                                 <  1:733621/151‑1 (MQ=255)

                                                                                                                 cGGAACCACCAGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATg                               >  1:703979/1‑151 (MQ=255)

                                                                                                                      ccaccaGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCCCTGGCTTTTTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGAAACCGTATCCCCCCGCCATCCCCATGCCGAt                          <  2:272104/151‑1 (MQ=255)

                                                                                                                        accaGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATg                         >  1:540562/1‑150 (MQ=255)

                                                                                                                           agacTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGAcc                      <  2:692240/147‑1 (MQ=255)

                                                                                                                           aGGCTATAGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCa                     <  2:463202/151‑1 (MQ=255)

                                                                                                                                ataGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGAc                  <  1:36479/149‑1 (MQ=255)

                                                                                                                                ataGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACg                 >  2:235648/1‑150 (MQ=255)

                                                                                                                                ataGACCTGAGCGGCAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACg                 <  2:671366/150‑1 (MQ=255)

                                                                                                                                              cAACGGCGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGt  <  2:108542/151‑1 (MQ=255)

TTACCTGCGTGTTTCTCTTTCCAGTCTTCCGCTTCCGGGATGTTTTTACGCAGCCAGAGAGCAAAGATGATTGGCAAAATGCCGATAAAGAACAGCGCACGCCAGCCCCAGACCGGAACCACCAGGCTATAGACCTGAGCGGCAACGACGGCCCCCACAGAGAAGCCTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGT  >  NC_000913/3371955‑3372247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A