Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F50 I1 R1	163	55.0	1534755	98.6%	1513268	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,373,078	T→C	T167A (ACC→GCC)	nanA ←	N‑acetylneuraminate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,373,078	0	T	C	100.0%	159.3 / NA	43	T167A (ACC→GCC)	nanA	N‑acetylneuraminate lyase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (28/15); total (28/15)

CGCCAGCCCATGATGTTGTAGGTACTGCCGATACCACCATCAGCGCCCGCCAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCA  >  NC_000913/3372930‑3373222

cgcCAGCCCATGATGTTGTAGGTACTGCCGATACCACCATCAGCGCCCGCCAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCt                                                                                                                                                >  2:428917/1‑151 (MQ=255)

cgcCAGCCCATGATGTTGTAGGTACTGCCGATACCACCATCAGCGCCCGCCAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCt                                                                                                                                                <  1:730348/151‑1 (MQ=255)

       ccATGATGTTGTAGGTACTGCCGATCCCCCCATCAGCGCCCGCCAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAg                                                                                                                                         <  1:298443/151‑1 (MQ=255)

           gatgTTGTAGGTACTGCCGATACCACCATCAGCGCCCGCCAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAgcgc                                                                                                                                      >  2:216330/1‑150 (MQ=255)

             tgttgtAGGTACTGCCGATACCACCATCAGCGCCCGCCAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCAcc                                                                                                                                   >  1:296004/1‑151 (MQ=255)

                  tAGGTACTGCCGATACCACCATCAGCGCCCGCCAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTTATAGATATCGCCAGAGGCCTGTTTCAGCGCACCTACg                                                                                                                               <  1:105117/150‑1 (MQ=255)

                    ggTACTGCCGATACCACCATCAGCGCCCGCCAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGcc                                                                                                                             >  1:374241/1‑150 (MQ=255)

                        cTGCCGATACCACCATCAGCGCCCGCCAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGc                                                                                                                         >  1:442867/1‑150 (MQ=255)

                          gCCGATACCACCATCAGCGCCCGCCAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAAt                                                                                                                      <  2:503233/151‑1 (MQ=255)

                           ccGATACCCCCATCAGCGCCCGCCAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATg                                                                                                                     <  2:95595/151‑1 (MQ=255)

                                       tCAGCGCCCGCCAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCAAGGCAATGTAACAAgtgtgt                                                                                                         >  2:377455/1‑151 (MQ=255)

                                        cAGCGCCCGCCAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAgtgtgt                                                                                                         <  2:516240/150‑1 (MQ=255)

                                             cccgccAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTtgatct                                                                                                   <  1:216338/151‑1 (MQ=255)

                                              ccgccAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTtgatctg                                                                                                  <  2:747937/151‑1 (MQ=255)

                                              ccgccAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTtgatctg                                                                                                  >  1:135935/1‑151 (MQ=255)

                                                          cAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTc                                                                                        >  2:108001/1‑149 (MQ=255)

                                                                cGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTAc                                                                                >  2:704269/1‑151 (MQ=255)

                                                                 gAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTAcc                                                                               >  1:697979/1‑151 (MQ=255)

                                                                   aGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAg                                                                                      <  1:84342/142‑1 (MQ=255)

                                                                   aGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAg                                                                                      >  2:84341/1‑142 (MQ=255)

                                                                    gATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTAcccc                                                                             >  2:322339/1‑150 (MQ=255)

                                                                          gtcgtAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAgg                                                                      >  1:314429/1‑151 (MQ=255)

                                                                               aaCCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTgg                                                                 >  1:63317/1‑151 (MQ=255)

                                                                               aaCCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTgg                                                                 >  2:275079/1‑151 (MQ=255)

                                                                                  cGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAAt                                                              >  2:623817/1‑151 (MQ=255)

                                                                                    ttATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAAtgt                                                            >  2:163813/1‑151 (MQ=255)

                                                                                                 gATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCAt                                               <  2:535407/151‑1 (MQ=255)

                                                                                                     aGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGc                                           >  1:517959/1‑151 (MQ=255)

                                                                                                       gATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCa                                          >  1:688619/1‑150 (MQ=255)

                                                                                                       gATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCa                                          >  1:658467/1‑150 (MQ=255)

                                                                                                          gTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAAcc                                      >  2:47399/1‑151 (MQ=255)

                                                                                                                   ggATCTGCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATccgccg                              >  1:115734/1‑150 (MQ=255)

                                                                                                                         gCTCCATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGaa                            <  1:612642/146‑1 (MQ=255)

                                                                                                                            ccATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCAAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCaataat                    >  2:576978/1‑151 (MQ=255)

                                                                                                                             cATCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAAtt                   >  1:668586/1‑151 (MQ=255)

                                                                                                                               tCTGATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGc                 >  1:745729/1‑151 (MQ=255)

                                                                                                                                  gATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGccc               >  1:497664/1‑150 (MQ=255)

                                                                                                                                  gATAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCg              <  1:47400/151‑1 (MQ=255)

                                                                                                                                   aTAGAGATCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCg              >  1:429851/1‑150 (MQ=255)

                                                                                                                                        gaTCGCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTg        <  2:374225/151‑1 (MQ=255)

                                                                                                                                            gCCAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCg    <  1:537513/151‑1 (MQ=255)

                                                                                                                                              cAGAGGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCa  <  2:457016/151‑1 (MQ=255)

                                                                                                                                               agagGCCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGc   >  2:233504/1‑149 (MQ=255)

CGCCAGCCCATGATGTTGTAGGTACTGCCGATACCACCATCAGCGCCCGCCAGCAGACCAGAGGCGAAGATTTCGTCGTAACCGTTATAGAGCACAAGATCAGGATGTTCACGACGGATCTGCTCCATCTGATAGAGATCGCCAGAGGTCTGTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCA  >  NC_000913/3372930‑3373222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A