Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F50 I1 R1	163	55.0	1534755	98.6%	1513268	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,591,608	T→C	M240V (ATG→GTG)	ugpB ←	sn‑glycerol‑3‑phosphate ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,591,608	0	T	C	100.0%	178.5 / NA	49	M240V (ATG→GTG)	ugpB	sn‑glycerol‑3‑phosphate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (22/27); total (22/27)

GCCTACGCCGTAGTTAAATTTGGCGTACTCGCGAATGTTGGCAAGAGAACCGGAAGAGGCGGTGGTCATCGCGCAATCACCGTTATAGAACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCATCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCAGC  >  NC_000913/3591462‑3591754

gCCTACGCCGTAGTTAAATTTGGCGTACTCGCGAATGTTGGCAAGAGAACCGGAAGAGGCGGTGGTCATCGCGCAATCACCGTTATAGAACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACctcc                                                                                                                                                >  1:415817/1‑151 (MQ=255)

gCCTACGCCGTAGTTAAATTTGGCGTACTCGCGAATGTTGGCAAGAGAACCGGAAGAGGCGGTGGTCATCGCGCAATCACCGTTATAGAACTTCTCGGTGGATTCATCCTTACGACCGACGAAGCTGAAGTCGCCCTTCTTGTTCACctcc                                                                                                                                                >  1:557180/1‑151 (MQ=255)

 ccTACGCCGTAGTTAAATTTGGCGTACTCGCGAATGTTGGCAAGAGAACCGGAAGAGGCGGTGGTCATCGCGCAATCACCGTTATAGAACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCCcctcct                                                                                                                                               <  2:79338/151‑1 (MQ=255)

     cGCCGTAGTTAAATTTGGCGTACTCGCGAATGTTGGCAAGAGAACCGGAAGAGGCGGTGGTCATCGCGCAATCACCGTTATAGAACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCg                                                                                                                                             >  1:337092/1‑149 (MQ=255)

       ccGTAGTTAAATTTGGCGTACTCGCGAATGTTGGCAAGAGAACCGGAAGAGGCGGTGGTCATCGCGCAATCACCGTTATAGAACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCa                                                                                                                                         <  2:160078/151‑1 (MQ=255)

                 aTTTGGCGTACTCGCGAATGTTGGCAAGAGAACCGGAAGAGGCGGTGGTCATCGCGCAATCACCGTTATAGAACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGAtgtg                                                                                                                               <  2:196155/151‑1 (MQ=255)

                    tGGCGTACTCGCGAATGTTGGCAAGAGAACCGGAAGAGGCGGTGGTCATCGCGCAATCACCGTTATAGAACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGtt                                                                                                                             <  2:558894/150‑1 (MQ=255)

                               cgAATGTTGGCAAGAGAACCGGAAGAGGCGGTGGTCATCGCGCAATCACCGTTATAGAACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCg                                                                                                                 <  2:19511/151‑1 (MQ=255)

                                    gTTGGCAAGAGAACCGGAAGAGGCGGTGGTCATCGCGCAATCACCGTTATAGAACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCtt                                                                                                             <  2:40402/150‑1 (MQ=255)

                                          aaGAGAACCGGAAGAGGCGGTGGTCATCGCGCAATCACCGTTATAGAACTTCTCGGGGGATTCCTCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTTACCTGCTCCGGCTTATTGaa                                                                                                       >  2:696222/1‑150 (MQ=255)

                                              gaACCGGAAGAGGCGGTGGTCATCGCGCAATCACCGTTATAGAACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCa                                                                                                  <  2:640696/151‑1 (MQ=255)

                                                aCCGGAAGAGGCGGTGGTCATCGCGCAATCACCGTTATAGAACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGc                                                                                                <  2:313612/151‑1 (MQ=255)

                                                   ggAAGAGGCGGTGGCCATCGCGCAATCAACGTTATAGAAATTCTCGGGGGGTTCATCCTTTAGACCGACGTAGCTGAAAGCGCCCTTCTTGGTTACCTCCTCGCGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTTAACTCCAGCAcc                                                                                             >  2:389634/1‑151 (MQ=255)

                                                        agGCGGTGGTCATCGCGCAATCACCGTTATAGAACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTc                                                                                        <  1:525783/151‑1 (MQ=255)

                                                         ggCGGTGGTCATCGCGCAATCACCGTTATAGAACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTcc                                                                                       <  2:530523/151‑1 (MQ=255)

                                                                gtCATCGCGCAATCACCGTTATAGAACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGt                                                                                <  1:117029/151‑1 (MQ=255)

                                                                gtCATCGCGCAATCACCGTTATAGAACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGt                                                                                <  1:31165/151‑1 (MQ=255)

                                                                       cgcAATCACCGTTATAGAACTTCTCGGTAGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCTCGTCCGTGCCGTCaaa                                                                            <  2:461713/148‑1 (MQ=255)

                                                                               ccGTTATAGAACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGtttt                                                                 >  2:675063/1‑151 (MQ=255)

                                                                                 gTTATAGAACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGttttt                                                                >  2:391666/1‑150 (MQ=255)

                                                                                  ttATAGAACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGc                                                              >  2:319855/1‑151 (MQ=255)

                                                                                    ataGCACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGACGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGGTATATGTTGCGCCTGCCCCGGCTTATTAACCTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCt                                                             <  2:323396/150‑1 (MQ=255)

                                                                                    ataGAACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCt                                                             <  1:203560/150‑1 (MQ=255)

                                                                                      aGAACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGc                                                          >  2:658867/1‑151 (MQ=255)

                                                                                       gAACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCa                                                         <  2:271863/151‑1 (MQ=255)

                                                                                        aaCTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCaa                                                        <  2:714077/151‑1 (MQ=255)

                                                                                         aCTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCaaa                                                       <  2:526900/151‑1 (MQ=255)

                                                                                         aCTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCaa                                                        >  2:478523/1‑150 (MQ=255)

                                                                                            tctcGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACg                                                     <  1:594674/150‑1 (MQ=255)

                                                                                               cGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACgg                                                    <  2:15966/148‑1 (MQ=255)

                                                                                                  tGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGAcc                                              >  1:437535/1‑151 (MQ=255)

                                                                                                        cATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTcc                                         >  2:267984/1‑150 (MQ=255)

                                                                                                         aTCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTcc                                         >  1:286417/1‑149 (MQ=255)

                                                                                                           ccTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGgc                                     <  2:724211/151‑1 (MQ=255)

                                                                                                               aCGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCt                                  >  2:217666/1‑150 (MQ=255)

                                                                                                                  accgacGTAGCTGAAGTCGCTCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTaa                                >  1:286049/1‑149 (MQ=255)

                                                                                                                     gacGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGgcg                                    >  1:685383/1‑142 (MQ=255)

                                                                                                                     gacGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGgcg                                    <  2:685341/142‑1 (MQ=255)

                                                                                                                     gacGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGttt                           >  2:511779/1‑151 (MQ=255)

                                                                                                                      acGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGtttt                          >  2:304706/1‑151 (MQ=255)

                                                                                                                      acGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTAGCAAACGGCAGACCGTTCCAGGCGCTAAAGtttt                          >  2:690804/1‑151 (MQ=255)

                                                                                                                       cGTAGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTc                         >  1:100196/1‑151 (MQ=255)

                                                                                                                        gTAGCTGAAGACGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTcc                        <  2:624875/151‑1 (MQ=255)

                                                                                                                          aGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGTGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCa                       <  1:433044/150‑1 (MQ=255)

                                                                                                                          aGCTGAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAg                      <  1:235344/151‑1 (MQ=255)

                                                                                                                              gAAGTCGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGtt                    <  1:4975/149‑1 (MQ=255)

                                                                                                                                   cGCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGATCCa             <  2:309667/151‑1 (MQ=255)

                                                                                                                                    gCCCTTCTTGTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGATcc              >  2:291539/1‑149 (MQ=255)

                                                                                                                                             gTTCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCa    <  1:172712/150‑1 (MQ=255)

                                                                                                                                              ttCACCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCAGc  >  2:381213/1‑151 (MQ=255)

GCCTACGCCGTAGTTAAATTTGGCGTACTCGCGAATGTTGGCAAGAGAACCGGAAGAGGCGGTGGTCATCGCGCAATCACCGTTATAGAACTTCTCGGTGGATTCATCCTTACGACCGACGTAGCTGAAGTCGCCCTTCTTGTTCATCTCCTCGAGCATGGCGATGTGTTTCACCTGCTCCGGCTTATTGAACTCCAGCACCGCGTCCGTGCCGTCAAAGCCGTTGTTTTTGCTGGCAAACGGCAGACCGTTCCAGGCGCTAAAGTTTTCCAGTTGGATCCAGCCCTGCCAGC  >  NC_000913/3591462‑3591754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A