Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F50 I1 R1	163	55.0	1534755	98.6%	1513268	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,983,841	A→G	Y295C (TAC→TGC)	aslB →	putative AslA‑specific sulfatase‑maturating enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,983,841	0	A	G	100.0%	184.2 / NA	51	Y295C (TAC→TGC)	aslB	putative AslA‑specific sulfatase‑maturating enzyme
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (31/20); total (31/20)

GACCACTGGATCAAACGCGACGTCGGCAAGATTTTCGTGATGAATATCGAATGGGCGTTTGCCAATTTTGTCGGTGCGCCGGGTGCGGTTTGCCATCATCAGCCAACCTGTGGGCGCTCGGTGATTGTTGAGCACAACGGCGACGTTTACGCCTGTGATCACTATGTTTATCCGCAATATCGGCTGGGGAATATGCACCAGCAAACAATTGCAGAAATGATCGATTCCCCGCAACAGCAGGCGTTTGGTGAAGATAAATTTAAGCAGTTACCGGCGCAGTGTCGCAGTTGTAA  >  NC_000913/3983693‑3983985

gACCACTGGATCAAACGCGACGTCGGCAAGATTTTCGTGATGAATATCGAATGGGCGTTTGCCAATTTTGTCGGTGCGCCGGGTGCGGTTTGCCATCATCAGCCAACCTGTGGGCGCTCGGTGATTGTTGAGCACAACGGCGACGTTTGCg                                                                                                                                                >  1:521441/1‑151 (MQ=255)

       ggATCAAACGCGACGTCGGCAAGATTTTCGTGATGAATATCGAATGGGCGTTTGCCAATTTTGTCGGTGCGCCGGGTGCGGTTTGCCATCATCAGCCAACCTGTGGGCGCTCGGTGATTGTTGAGCACAACGGCGACGTTTGCGCCTGTGa                                                                                                                                         <  2:740353/151‑1 (MQ=255)

             aaCGCGACGTCGGCAAGATTTTCGTGATGAATATCGAATGGGCGTTTGCCAATTTTGTCGGTGCGCCGGGTGCGGTTTGCCATCATCAGCCAACCTGTGGGCGCTCGGTGATTGTTGAGCACAACGGCGACGTTTGCGCCTGTGATCACt                                                                                                                                    >  1:479200/1‑150 (MQ=255)

                  gACGTCGGCAAGATTTTCGTGATGAATATCGAATGGGCGTTTGCCAATTTTGTCGGTGCGCCGGGTGCGGTTTGCCATCATCAGCCAACCTGTGGGCGCTCGGTGATTGTTGAGCACAACGGCGACGTTTGCGCCTGTGATCACTATGttt                                                                                                                              >  1:266507/1‑151 (MQ=255)

                      tCGGCAAGATTTTCGTGATGAATATCGAATGGGCGTTTGCCAATTTTGTCGGTGCGCCGGGTGCGGTTTGCCATCATCAGCCAACCTGTGGGCGCTCGGTGATTGTTGAGCACAACGGCGACGTTTGCGCCTGTGATCACTATGTTTATcc                                                                                                                          >  1:209034/1‑151 (MQ=255)

                           aaGATTTTCGTGATGAATATCGAATGGGCGTTTGCCAATTTTGTCGGTGCGCCGGGTGCGGTTTGCCATCATCAGCCAACCTGTGGGCGCTCGGTGATTGTTGAGCACAACGGCGACGTTTGCGCCTGTGATCACTATGTTTATCCGCAat                                                                                                                     >  1:608045/1‑151 (MQ=255)

                            aGATTTTCGTGATGAATATCGAATGGGCGTTTGCCAATTTTGTCGGTGCGCCGGGTGCGGTTTGCCATCATCAGCCAACCTGTGGGCGCTCGGTGATTGTTGAGCACAACGGCGACGTTTGCGCCTGTGATCACTATGTTTATCCGCAat                                                                                                                     >  2:664554/1‑150 (MQ=255)

                                tttCGTGATGAATATCGAATGGGCGTTTGCCAATTTTGTCGGTGCGCCGGGTGCGGTTTGCCATCATCAGCCAACCTGTGGGCGCTCGGTGATTGTTGAGCACAACGGCGACGTTTGCGCCTGTGATCACTATGTTTATCCGCAATATCg                                                                                                                 <  2:640609/150‑1 (MQ=255)

                                  tCGTGATGAATATCGAATGGGCGTTTGCCAATTTTGTCGGTGCGCCGGGTGCGGTTTGCCATCATCAGCCAACCTGTGGGCGCTCGGTGATTGTTGAGCACAACGGCGACGTTTGCGCCTGTGATCACTATGTTTATCCGCAATATCgg                                                                                                                <  2:622115/149‑1 (MQ=255)

                                  tCGTGATGAATATCGAATGGGCGTTTGCCAATTTTGTCGGTGCGCCGGGTGCGGTTTGCCATCATCAGCCAACCTGTGGGCGCTCGGTGATTGTTGAGCACAACGGCGACGTTTGCGCCTGTGATCACTATGTTTATCCGCAATATCGGc                                                                                                               <  2:591351/150‑1 (MQ=255)

                                   cGTGATGAATATCGAATGGGCGTTTGCCAATTTTGTCGGTGCGCCGGGTGCGGTTTGCCATCATCAGCCAACCTGTGGGCGCTCGGTGATTGTTGAGCACAACGGCGACGTTTGCGCCTGTGATCACTATGTTTATCCGCAATATCGGCTg                                                                                                             >  1:633937/1‑151 (MQ=255)

                                              tCGAATGGGCGTTTGCCAATTTTGTCGGTGCGCCGGGTGCGGTTTGCCATCATCAGCCAACCTGTGGGCGCTCGGTGATTGTTGAGCACAACGGCGACGTTTGCGCCTGTGATCACTATGTTTATCCGCAATATCGGCTGGGGAATATGc                                                                                                   >  2:133532/1‑150 (MQ=255)

                                                 aaTGGGCGTTTGCCAATTTTGTCGGTGCGCCGGGTGCGGTTTGCCATCATCAGCCAACCTGTGGGCGCTCGGTGATTGTTGAGCACAACGGCGACGTTTGCGCCTGTGATCACTATGTTTATCCGCAATATCGGCTGGGGAATATGCACCa                                                                                               <  1:213809/151‑1 (MQ=255)

                                                      gCGTTTGCCAATTTTGTCGGTGCGCCGGGTGCGGTTTGCCATCATCAGCCAACCTGTGGGCGCTCGGTGATTGTTGAGCACAACGGCGACGTTTGCGCCTGTGATCACTATGTTTATCCGCAATATCGGCTGGGGAATATGCACCAGCaa                                                                                           >  2:322718/1‑150 (MQ=255)

                                                      gCGTTTGCCAATTTTGTCGGTGCGCCGGGTGCGGTTTGCCATCATCAGCCAACCTGTGGGCGCTCGGTGATTGTTGAGCACAACGGCGACGTTTGCGCCTGTGATCACTATGTTTATCCGCAATATCGGCTGGGGAATATGCACCAGCa                                                                                            >  1:449492/1‑149 (MQ=255)

                                                           tGCCAATTTTGTCGGTGCGCCGGGTGCGGTTTGCCATCATCAGCCAACCTGTGGGCGCTCGGTGATTGTTGAGCACAACGGCGACGTTTGCGCCTGTGATCACTATGTTTATCCGCAATATCGGCTGGGGAATATGCACCAGCAAACAAt                                                                                      <  2:160098/150‑1 (MQ=255)

                                                            gCCAATTTTGTCGGTGCGCCGGGTGCGGTTTGCCATCATCAGCCAACCTGTGGGCGCTCGGTGATTGTTGAGCACAACGGCGACGTTTGCGCCTGTGATCACTATGTTTATCCGCAATATCGGCTGGGGAATATGCACCAGCAAACAATTg                                                                                    >  2:335747/1‑151 (MQ=255)

                                                             ccAATTTTGTCGGTGCGCCGGGTGCGGTTTGCCATCATCAGCCAACCTGTGGGCGCTCGGTGATTGTTGAGCACAACGGCGACGTTTGCGCCTGTGATCACTATGTTTATCCGCAATATCGGCTGGGGAATATGCACCAGCAAACAATTGc                                                                                   <  2:98066/151‑1 (MQ=255)

                                                              cAATTTTGTCGGTGCGCCGGGTGCGGTTTGCCATCATCAGCCAACCTGTGGGCGCTCGGTGATTGTTGAGCACAACGGCGACGTTTGCGCCTGTGATCACTATGTTTATCCGCAATATCGGCTGGGGAATATGCACCAGCAAACAATTGCa                                                                                  <  1:524053/151‑1 (MQ=255)

                                                               aaTTTTGTCGGTGCGCCGGGTGCGGTTTGCCATCATCAGCCAACCTGTGGGCGCTCGGTGATTGTTGAGCACAACGGCGACGTTTGCGCCTGTGATCACTATGTTTATCCGCAATATCGGCTGGGGAATATGCACCAGCAAACAATTGc                                                                                   >  1:107577/1‑149 (MQ=255)

                                                                aTTTTGTCGGTGCGCCGGGTGCGGTTTGCCATCATCAGCCAACCTGTGGGCGCTCGGTGATTGTTGAGCACAACGGCGACGTTTGCGCCTGTGATCACTATGTTTATCCGCAATATCGGCTGGGGAATATGCACCAGCAAACAATTGCAGa                                                                                <  2:534715/151‑1 (MQ=255)

                                                                  tttGTCGGTGCGCCGGGTGCGGTTTGCCATCATCAGCCAACCTGTGGGCGCTCGGTGATTGTTGAGCACAACGGCGACGTTTGCGCCTGTGATCACTATGTTTATCCGCAATATCGGCTGGGGAATATGCACCAGCAAACAATTGCAGaa                                                                               <  2:72101/150‑1 (MQ=255)

                                                                    tGTCGGTGCGCCGGGTGCGGTTTGCCATCATCAGCCAACCTGTGGGCGCTCGGTGATTGTTGAGCACAACGGCGACGTTTGCGCCTGTGATCACTATGTTTATCCGCAATATCGGCTGGGGAATATGCACCAGCAAACAATTGCAGAAATg                                                                            >  2:760828/1‑151 (MQ=255)

                                                                     gTCGGTGCGCCGGGTGCGGTTTGCCATCATCAGCCAACCTGTGGGCGCTCGGTGATTGTTGAGCACAACGGCGACGTTTGCGCCTGTGATCACTATGTTTATCCGCAATATCGGCTGGGGAATATGCACCAGCAAACAATTGCAGAAATg                                                                            >  2:761885/1‑150 (MQ=255)

                                                                      tCGGTGCGCCGGGTGCGGTTTGCCATCATCAGCCAACCTGTGGGCGCTCGGTGATTGTTGTGCACAACGGCGACGTTTGCGCCTGTGATCACTATGTTTATCCGCAATATCGGCTGGGGAATATGCACCAGCAAACAATTGCAGAAATg                                                                            >  2:665572/1‑149 (MQ=255)

                                                                         gTGCGCCGGGTGCGGTTTTCCATCATCAGCCAACCTGTGGGCGCTCGGTGATTGTTGAGCACAACGGCGACGTTTGCGCCGGTGATCACTATGTTTATCCGCAATATCGGCTGGGGAATATGCACCAGCAAACAATTGCAGAAATGa                                                                           >  2:767198/1‑147 (MQ=255)

                                                                          tGCGCCGGGTGCGGTTTGCCATCATCAGCCAACCTGTGGGCGCTCGGTGATTGTTGAGCACAACGGCGACGTTTGCGCCTGTGATCACTATGTTTATCCGCAATATCGGCTGGGGAATATGCACCAGCAAACAATTGCAGAAATGATCGAt                                                                      >  1:394782/1‑151 (MQ=255)

                                                                                        tttGCCATCATCAGCCAACCTGTGGGCGCTCGGTGATTGTTGAGCACAACGGCGACGTTTGCGCCTGTGATCACTATGTTTATCCGCAATATCGGCTGGGGAATATGCACCAGCAAACAATTGCAGAAATGATCGATTCCCCGCAAcagc                                                         >  1:703459/1‑150 (MQ=255)

                                                                                             catcatCAGCCAACCTGTGGGCGCTCGGTGATTGTTGAGCACAACGGCGACGTTTGCGCCTGTGATCACTATGTTTATCCGCAATATCGGCTGGGGAATATGCACCAGCAAACAATTGCAGAAATGATCGATTCCCCGCAACAGCAGGCGt                                                   <  1:567865/151‑1 (MQ=255)

                                                                                                catcaGCCAACCTGTGGGCGCTCGGTGATTGTTGAGCACAACGGCGACGTTTGCGCCTGTGATCACTATGTTTATCCGCAATATCGGCTGGGGAATATGCACCAGCAAACAATTGCAGAAATGAACGATTCCCCGCAACAGCAGGCGttt                                                 <  1:632481/150‑1 (MQ=255)

                                                                                                  tcaGCCAACCTGTGGGCGCTCGGTGATTGTTGAGCACAACGGCGACGTTTGCGCCTGTGATCACTATGTTTATCCGCAATATCGGCTGGGGAATATGCACCAGCAAACAATTGCAGAAATGATCGATTCCCCGCAACAGCAGGCGTTTGGt                                              >  2:523706/1‑151 (MQ=255)

                                                                                                   caGCCAACCTGTGGGCGCTCGGTGATTGTTGAGCACAACGGCGACGTTTGCGCCTGTGATCACTATGTTTATCCGCAATATCGGCTGGGGAATATGCACCAGCAAACAATTGCAGAAATGATCGATTCCCCGCAACAGCAGGCGTTTGGt                                              <  2:132262/150‑1 (MQ=255)

                                                                                                   caGCCAACCTGTGGGCGCTCGGTGATTGTTGAGCACAACGGCGACGTTTGCGCCTGTGATCACTATGTTTATCCGCAATATCGGCTGGGGAATATGCACCAGCAAACAATTGCAGAAATGATCGATTCCCCGCAACAGCAGGCGTTTGGTg                                             >  1:38361/1‑151 (MQ=255)

                                                                                                    aGCCAACCTGTGGGCGCTCGGTGATTGTTGAGCACAACGGCGACGTTTGCGCCTGTGATCACTATGTTTATCCGCAATATCGGCTGGGGAATATGCACCAGCAAACAATTGCAGAAATGATCGATTCCCCGCAACAGCAGGCGTTTGGTg                                             >  2:674376/1‑150 (MQ=255)

                                                                                                    aGCCAACCTGTGGGCGCTCGGTGATTGTTGAGCACAACGGCGACGTTTGCGCCTGTGATCACTATGTTTATCCGCAATATCGGCTGGGGAATATGCACCAGCAAACAATTGCAGAAATGATCGATTCCCCGCAACAGCAGGCGTTTGGTGa                                            <  2:685874/151‑1 (MQ=255)

                                                                                                        aaCCTGTGGGCGCTCGGTGATTGTTGAGCACAACGGCGACGTTTGCGCCTGTGATCACTATGTTTATCCGCAATATCGGCTGGGGAATATGCACCAGCAAACAATTGCAGAAATGATCGATTCCCCGCAACAGCAGGCGTTTGGTGa                                            >  1:181496/1‑147 (MQ=255)

                                                                                                           cTGTGGGCGCTCGGTGATTGTTGAGCACAACGGCGACGGTTGCGCCTGTGATCACTATGTTTATCCGCAATATCGGCTGGGGAATATGCACCAGCAAACAATTGCAGAAATGATCGATTCCCCGCAACATCAGGCGTTTGGAGAAGATaa                                      >  2:201618/1‑150 (MQ=255)

                                                                                                             gtgGGCGCTCGGTGATTGTTGAGCACAACGGCGACGTTTGCGCCTGTGATCACTATGTTTATCCGCAATATCGGCTGGGGAATATGCACCAGCAAACAATTGCAGAAATGATCGATTCCCCGCAACAGCAGGCGTTTGGTGAAGATaaa                                     <  2:214124/149‑1 (MQ=255)

                                                                                                               gggCGCTCGGTGATTGTTGAGCACAACGGCGACGTTTGCGCCTGTGATCACTATGTTTATCCGCAATATCGGCTGGGGAATATGCACCAGCAAACAATTGCAGAAATGATCGATTCCCCGCAACAGCAGGCGTTTGGTGAAGATAAATTTa                                 <  2:617215/151‑1 (MQ=255)

                                                                                                                   gcTCGGTGATTGTTGAGCACAACGGCGACGTTTGCGCCTGTGATCACTATGTTTATCCGCAATATCGGCTGGGGAATATGCACCAGCAAACAATTGCAGAAATGATCGATTCCCCGCAACAGCAGGCGTTTGGTGAAGATAAATTTAAGc                              >  1:569506/1‑150 (MQ=255)

                                                                                                                      cGGTGATTGTTGAGCACAACGGCGACGTTTGCGCCTGTGATCACTATGTTTATCCGCAATATCGGCTGGGGAATATGCACCAGCAAACAATTGCAGAAATGATCGATTCCCCGCAACAGCAGGCGTTTGGTGAAGATAAATTTAAGCAGtt                          >  1:669280/1‑151 (MQ=255)

                                                                                                                          gATTGTTGAGCACAACGGCGACGTTTGCGCCTGTGATCACTATGTTTATCCGCAATATCGGCTGGGGAATATGCACCAGCAAACAATTGCAGAAATGATCGATTCCCCGCAACAGCAGGCGTTTGGTGAAGATAAATTTAAGCAGTTAcc                       <  1:373408/150‑1 (MQ=255)

                                                                                                                          gATTGTTGAGCACAACGGCGACGTTTGCGCCTGTGATCACTATGTTTATCCGCAATATCGGCTGGGGAATATGCACCAGCAAACAATTGCAGAAATGATCGATTCCCCGCAACAGCAGGCGTTTGGTGAAGATAAATTTAAGCAGTTACCg                      >  1:617761/1‑151 (MQ=255)

                                                                                                                            ttgttgAGCACAACGGCGACGTTTGCGCCTGTGATCACTATGTTTATCCGCAATATCGGCTGGGGAATATGCACCAGCAAACAATTGCAGAAATGATCGATTCCCCGCAACAGCAGGCGTTTGGTGAAGATAAATTTAAGCAGTTACCGgc                    >  2:421862/1‑151 (MQ=255)

                                                                                                                              gttgAGCACAACGGCGACGTTTGCGCCTGTGATCACTATGTTTATCCGCAATATCGGCTGGGGAATATGCACCAGCAAACAATTGCAGAAATGATCGATTCCCCGCAACAGCAGGCGTTTGGTGAAGATAAATTTAAGCAGTTACCGgcgc                  <  1:76305/151‑1 (MQ=255)

                                                                                                                                 gAGCACAACGGCGACGTTTGCGCCTGTGATCACTATGTTTATCCGCAATATCGGCTGGGGAATATGCACCAGCAAACAATTGCAGAAATGATCGATTCCCCGCAACAGCAGGCGTTTGGTGAAGATAAATTTAAGCAGTTACCGGCGCAgt               >  1:463770/1‑151 (MQ=255)

                                                                                                                                     acaACGGCGACGTTTGCGCCTGTGATCACTATGTTTATCCGCAATATCGGCTGGGGAATATGCACCAGCAAACAATTGCAGAAATGATCGATTCCCCGCAACAGCAGGCGTTTGGTGAAGATAAATTTAAGCAGTTACCGGCGCAGTGTCg           >  1:82412/1‑151 (MQ=255)

                                                                                                                                      caACGGCGACGTTTGCGCCTGTGATCACTATGTTTATCCGCAATATCGGCTGGGGAACATGCACCAGCAAACAATTGCAGAAATGATCGATTCCCCGCAACAGCAGGCGTTTGGTGAAGATAAATTTAAGCAGTTACCGGCGCAGTGTCGc          <  1:756251/151‑1 (MQ=255)

                                                                                                                                         cGGCGACGTTTGCGCCTGTGATCACTATGTTTATCCGCAATATCGGCTGGGGAATATGCACCAGCAAACAATTGCAGAAATGATCGATTCCCCGCAACAGCAGGCGTTTGGTGAAGATAAATTTAAGCAGTTACCGGCGCAGTGTCGCAGt       >  2:20402/1‑151 (MQ=255)

                                                                                                                                             gACGTTTGCGCCTGTGATCACTATGTTTATCCGCAATATCGGCTGGGGAATATGCACCAGCAAACAATTGCAGAAATGATCGATTCCCCGCAACAGCAGGCGTTTGGTGAAGATAAATTTAAGCAGTTACCGGCGCAGGGTCGCAGTTGt    >  2:352357/1‑150 (MQ=255)

                                                                                                                                              aCGTTTGCGCCTGTGATCACTATGTTTATCCGCAATATCGGCTGGGGAATATGCACCAGCAAACAATTGCAGAAATGATCGATTCCCCGCAACAGCAGGCGTTTGGTGAAGATAAATTTAAGCAGTTACCGGCGCAGTGTCGCAGTTGTaa  <  1:155830/151‑1 (MQ=255)

GACCACTGGATCAAACGCGACGTCGGCAAGATTTTCGTGATGAATATCGAATGGGCGTTTGCCAATTTTGTCGGTGCGCCGGGTGCGGTTTGCCATCATCAGCCAACCTGTGGGCGCTCGGTGATTGTTGAGCACAACGGCGACGTTTACGCCTGTGATCACTATGTTTATCCGCAATATCGGCTGGGGAATATGCACCAGCAAACAATTGCAGAAATGATCGATTCCCCGCAACAGCAGGCGTTTGGTGAAGATAAATTTAAGCAGTTACCGGCGCAGTGTCGCAGTTGTAA  >  NC_000913/3983693‑3983985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A