Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F50 I1 R1 163 55.0 1534755 98.6% 1513268 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,408,885 (A)6→5 coding (2232/2442 nt) rnr → exoribonuclease R, RNase R

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,408,8800A.100.0% 210.0 / NA 43coding (2227/2442 nt)rnrexoribonuclease R, RNase R
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (18/25);  total (18/25)

GGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGTAAAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAACGAAGCCGAAAGCGGCGAAGAAAGACGCGAGAAAAGCGAAAAAGCCAT  >  NC_000913/4408730‑4409024
                                                                                                                                                      |                                                                                                                                                
ggggAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGa                                                                                                                                                  <  2:310632/151‑1 (MQ=255)
 gggAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGa                                                                                                                                                  >  2:341046/1‑150 (MQ=255)
   gAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGaaaa                                                                                                                                               <  1:627167/151‑1 (MQ=255)
         aGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCG‑AAAAAGGCGa                                                                                                                                        <  2:243788/151‑1 (MQ=255)
             gCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCG‑AAAAAGGCGATGCa                                                                                                                                    <  1:342585/151‑1 (MQ=255)
               cAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCG‑AAAAAGGCGATGCAgg                                                                                                                                  >  2:505276/1‑151 (MQ=255)
                      aTCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCG‑AAAAAGGCGATGCAGGTaaaaa                                                                                                                            <  1:133075/150‑1 (MQ=255)
                      aTCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCG‑AAAAAGGCGATGCAGGTaaaaa                                                                                                                            >  2:601700/1‑150 (MQ=255)
                       tCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCG‑AAAAAGGCGATGCAGGTaaaaa                                                                                                                            >  1:762341/1‑149 (MQ=255)
                        cGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCG‑AAAAAGGCGATGCAGGTAAAAAAgg                                                                                                                         >  2:574568/1‑151 (MQ=255)
                             gggCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCTCCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCG‑AAAAAGGCGATGCAGGTAAAAAAGGCGGCa                                                                                                                    <  2:386870/151‑1 (MQ=255)
                               gCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCG‑AAAAAGGCGATGCAGGTAAAAAAGGCGGCAAg                                                                                                                  <  2:500567/151‑1 (MQ=255)
                                cGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCG‑AAAAAGGCGATGCAGGTAAAAAAGGCGGCAAGc                                                                                                                 <  1:620081/151‑1 (MQ=255)
                                  aTCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCG‑AAAAAGGCGATGCAGGTAAAAAAGGCGGCAAGcg                                                                                                                <  1:71683/150‑1 (MQ=255)
                                   tCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCG‑AAAAAGGCGATGCAGGTAAAAAAggc                                                                                                                        >  1:38248/1‑141 (MQ=255)
                                   tCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCG‑AAAAAGGCGATGCAGGTAAAAAAggc                                                                                                                        <  2:38247/141‑1 (MQ=255)
                                   tCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCG‑AAAAAGGCGATGCAGGTAAAAAAGGCGGCAAGcgtc                                                                                                              >  1:763054/1‑151 (MQ=255)
                                     gcgTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCG‑AAAAAGGCGATGCAGGTAAAAAAGGCGGCAAGcgtcgt                                                                                                            <  1:289687/151‑1 (MQ=255)
                                     gcgTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCG‑AAAAAGGCGATGCAGGTAAAAAAGGCGGCAAGcgtcgt                                                                                                            >  1:693668/1‑151 (MQ=255)
                                       gTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCG‑AAAAAGGCGATGCAGGTAAAAAAGGCGGCAAGCGTCGTCa                                                                                                          <  1:583057/151‑1 (MQ=255)
                                             gTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCG‑AAAAAGGCGATGCAGGTAAAAAAGGCGGCAAGCGTCGTCAGGTCgg                                                                                                    >  1:112313/1‑151 (MQ=255)
                                                  cgTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCG‑AAAAAGGCGATGCAGGTAAAAAAGGCGGCAAGCGTCGTCAGGTCGGTaaa                                                                                                >  2:755088/1‑150 (MQ=255)
                                                  cgTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCG‑AAAAAGGCGATGCAGGTAAAAAAGGCGGCAAGCGTCGTCAGGTCGGTaaa                                                                                                >  1:541528/1‑150 (MQ=255)
                                                   gTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCG‑AAAAAGGCGATGCAGGTAAAAAAGGCGGCAAGCGTCGTCAGGTCGGTaaaaa                                                                                              <  1:329522/151‑1 (MQ=255)
                                                     cGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCG‑AAAAAGGCGATGCAGGTAAAAAAGGCGGTAAGCGTCGTCAGGTCGGTAAAAAgg                                                                                            <  2:588666/151‑1 (MQ=255)
                                                      gAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCG‑AAAAAGGCGATGCAGGTAAAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAgg                                                                                            >  1:169803/1‑150 (MQ=255)
                                                        aGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCG‑AAAAAGGCGATGCAGGTAAAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTa                                                                                          >  1:670030/1‑150 (MQ=255)
                                                           ggTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCG‑AAAAAGGCGATGCAGGTAAAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACt                                                                                      <  1:266002/151‑1 (MQ=255)
                                                               aaTATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCG‑AAAAAGGCGATGCAGGTAAAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGa                                                                                  <  1:482178/151‑1 (MQ=255)
                                                                  atGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCG‑AAAAAGGCGATGCAGGTAAAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGcc                                                                               >  2:535493/1‑151 (MQ=255)
                                                                         aGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCG‑AAAAAGGCGATGCAGGTAAAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAgc                                                                        <  2:462456/151‑1 (MQ=255)
                                                                             cAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCG‑AAAAAGGCGATGCAGGTAAAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCt                                                                    >  2:697117/1‑151 (MQ=255)
                                                                             cAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCG‑AAAAAGGCGATGCAGGTAAAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCt                                                                    <  1:442230/151‑1 (MQ=255)
                                                                               aaaTCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCG‑AAAAAGGCGATGCAGGTAAAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTc                                                                  <  1:35104/151‑1 (MQ=255)
                                                                                  tCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCG‑AAAAAGGCGATGCAGGTAAAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCcgc                                                               >  1:328573/1‑151 (MQ=255)
                                                                                                aTCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCG‑AAAAAGGCGATGCAGGTAAAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAAc                                                 >  1:706092/1‑151 (MQ=255)
                                                                                                     cAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCG‑AAAAAGGCGATGCAGGTAAAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAAc                                                 <  2:166072/146‑1 (MQ=255)
                                                                                                       gCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCG‑AAAAAGGCGATGCAGGTAAAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAACGAAGCCg                                          <  1:441593/151‑1 (MQ=255)
                                                                                                           aCGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCG‑AAAAAGGCGATGCAGGTAAAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAACGAAGCCGAAAg                                      <  1:261945/151‑1 (MQ=255)
                                                                                                                    gcgcAACGTCGGTAAAACGGCGCGCGAGAAAGCG‑AAAAAGGCGATGCAGGTAAAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAACGAAGCCGAAAGCGGCgaaga                             <  2:367321/151‑1 (MQ=255)
                                                                                                                        aaCGTCGGTAAAACGGCGCGCGAGAAAGCG‑AAAAAGGCGATGCAGGTAAAAAAAGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAACGAAGCCGAAAGCGGCgaagaa                            >  2:710017/1‑148 (MQ=255)
                                                                                                                         aCGTCGGTAAAACGGCGCGCGAGAAAGCG‑AAAAAGGCGATGCAGGTAAAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAACGAAGCCGAAAGCGGCGAAGAAAGAc                        <  1:227584/151‑1 (MQ=255)
                                                                                                                         aCGTCGGTAAAACGGCGCGCGAGAAAGCG‑AAAAAGGCGATGCAGGTAAAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAACGAAGCCGAAAGCGGCGAAGAAAGAc                        <  2:293093/151‑1 (MQ=255)
                                                                                                                                    aCGGCGCGCGAGAAAGCG‑AAAAAGGCGATGCAGGTAAAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAACGAAGCCGAAAGCGGCGAAGAAAGACGCGAGAAAAGc             >  2:727632/1‑151 (MQ=255)
                                                                                                                                     cGGCGCGCGAGAAAGCG‑AAAAAGGCGATGCAGGTAAAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAACGAAGCCGAAAGCGGCGAAGAAAGACGCGAGAAAAGCg            <  1:666193/151‑1 (MQ=255)
                                                                                                                                               gaAAGCG‑AAAAAGGCGATGCAGGTAAAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAACGAAGCCGAAAGCGGCGAAGAAAGACGCGAGAAAAGCGAAAAAGCCAt  <  1:544985/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                
GGGGAATCCAGCGGCCAGACTTATCGCCTGGGCGATCGCGTGGAAGTTCGCGTCGAAGCGGTTAATATGGACGAGCGCAAAATCGACTTTAGCCTGATCTCCAGCGAACGCGCACCGCGCAACGTCGGTAAAACGGCGCGCGAGAAAGCGAAAAAAGGCGATGCAGGTAAAAAAGGCGGCAAGCGTCGTCAGGTCGGTAAAAAGGTAAACTTTGAGCCAGACAGCGCCTTCCGCGGTGAGAAAAAAACGAAGCCGAAAGCGGCGAAGAAAGACGCGAGAAAAGCGAAAAAGCCAT  >  NC_000913/4408730‑4409024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A