Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F50 I1 R1 163 55.0 1534755 98.6% 1513268 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,431,878 T→C T36A (ACG→GCG)  ytfE ← iron‑sulfur cluster repair protein RIC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,431,8780TC100.0% 97.7 / NA 31T36A (ACG→GCG) ytfEiron‑sulfur cluster repair protein RIC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/27);  total (4/27)

CGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATG  >  NC_000913/4431743‑4432015
                                                                                                                                       |                                                                                                                                         
cGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCcac                                                                                                                             <  1:762708/150‑1 (MQ=255)
    gatATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCAcagcag                                                                                                                        <  1:663686/151‑1 (MQ=255)
                              gggCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACag                                                                                              <  1:185435/151‑1 (MQ=255)
                                 cgcTCCGCCGGTCTTTCTCAATCGGTTGTTCAGCGGGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCATTTCTTTACGTCCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACaga                                                                                             <  2:6829/149‑1 (MQ=255)
                                 cgcTACGCCAGTCTTTCTCAATCGGTTGTGCAGCGAGCTTTGCCAGTTCAGCTTCAAGGACCTCAACATCCAGTTCTTTACCTGCCGCCGCGCGCCCCAGCGCATGCTTACCGCCACAGCAGTAATCCATATCATATTTCCGAAACagag                                                                                            <  2:374706/150‑1 (MQ=255)
                                    tACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTg                                                                                         >  1:243007/1‑150 (MQ=255)
                                     aCGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCGGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGa                                                                                        <  2:479037/150‑1 (MQ=255)
                                         cAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAgcgc                                                                                   <  1:190881/151‑1 (MQ=255)
                                               ttCTCAATCGTTTGTTCAGCGAGCTTTGCAAGTTCAGCTTCAATGTCCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCGAGCGCCTGCTTGCCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAg                                                                                <  2:166145/148‑1 (MQ=255)
                                                tctcAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGCCCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGaa                                                                             <  2:318031/150‑1 (MQ=255)
                                                  tcAATCGGTTGTTCAGCAAGCTTTGCCAGTTCAGCTTCAATGACCTAAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAAt                                                                            <  1:540352/149‑1 (MQ=255)
                                                  tcAATCGGTTGTTCAGCAAGCTTTGCCAGTTCAGCTTCAATAACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGaa                                                                             >  2:540324/1‑148 (MQ=255)
                                                   cAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATag                                                                          <  1:740167/150‑1 (MQ=255)
                                                       cGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAgc                                                                     <  1:143841/151‑1 (MQ=255)
                                                        ggTTTTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATagaga                                                                       <  1:358826/148‑1 (MQ=255)
                                                           tgttCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGcc                                                                  <  1:104145/150‑1 (MQ=255)
                                                            gttCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCa                                                                 <  2:401829/150‑1 (MQ=255)
                                                                         tGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAgg                                                    <  1:334254/150‑1 (MQ=255)
                                                                                                cAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTg                             >  2:68539/1‑150 (MQ=255)
                                                                                                     tCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATAcc                        <  2:413887/150‑1 (MQ=255)
                                                                                                     tCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCt                       >  2:240427/1‑151 (MQ=255)
                                                                                                      ccAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCt                       <  1:434356/150‑1 (MQ=255)
                                                                                                          ttCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCAtt                   <  1:129874/150‑1 (MQ=255)
                                                                                                          ttCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCAt                    <  1:385320/149‑1 (MQ=255)
                                                                                                           tCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTc                  <  2:501871/150‑1 (MQ=255)
                                                                                                           tCTTTACGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTc                  <  2:199391/150‑1 (MQ=255)
                                                                                                                aCGTGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTtaat             <  2:672607/150‑1 (MQ=255)
                                                                                                                aCGGGCCGCCGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTtaata            <  2:400379/151‑1 (MQ=255)
                                                                                                                   tGCCGCCGCGCGCGCCAGCGCATGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAataag          <  2:409976/150‑1 (MQ=255)
                                                                                                                    gccgccGCGCGCGCCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAataagat        <  2:250384/151‑1 (MQ=255)
                                                                                                                          gcgcgcgcCAGCGCCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATg  <  2:398507/151‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                         
CGATGATATGGTCGATGATTTCTGCCAGCGGGGCGCTACGCCAGTCTTTCTCAATCGGTTGTTCAGCGAGCTTTGCCAGTTCAGCTTCAATGACCTCAACATCCAGTTCTTTACGTGCCGCCGCGCGCGCCAGCGTCTGCTTACCGCCACAGCAGTAATCCATATCATATTTACGAAACAGAGCTGAAGCGCGAGGAATAGAGAGCGCCAGTTCACCTAAAGGTTGGTCGCGATAAGCCATAGCTGATACCTCATTCTTAATAAGATAAGATG  >  NC_000913/4431743‑4432015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A