Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F50 I1 R1	163	55.0	1534755	98.6%	1513268	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,571,430	T→C	T49A (ACG→GCG)	yjiR ←	putative DNA‑binding transcriptional regulator/putative aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,571,430	0	T	C	100.0%	94.3 / NA	25	T49A (ACG→GCG)	yjiR	putative DNA‑binding transcriptional regulator/putative aminotransferase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (2/23); total (2/23)

CCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCT  >  NC_000913/4571301‑4571577

cccACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGCCTGATACGCCTGCTGCACgg                                                                                                                                 <  1:323517/150‑1 (MQ=255)

          aaTTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGCCTGATACGCCTGCTGCACGGTGCTGATACTg                                                                                                                      <  1:638792/151‑1 (MQ=255)

                cACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGCCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCg                                                                                                                <  2:564648/151‑1 (MQ=255)

                  ccGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGCCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTg                                                                                                              <  1:659106/151‑1 (MQ=255)

                             aCCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGCCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTa                                                                                                   <  2:280993/151‑1 (MQ=255)

                                 gACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGCCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCt                                                                                               <  1:130379/151‑1 (MQ=255)

                                 gACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGCCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCt                                                                                               <  1:185667/151‑1 (MQ=255)

                                       tCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATATCCCGAACGCGGCTTCGGAGTGATGAGCTTCATCGTCTCCAGCGCCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCa                                                                                          <  1:385916/150‑1 (MQ=255)

                                           cggcggTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGCCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGa                                                                                      <  2:571310/150‑1 (MQ=255)

                                                  aCTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGCCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAAtt                                                                              >  1:749183/1‑151 (MQ=255)

                                                          cTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGCCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCAc                                                                                         >  1:560990/1‑132 (MQ=255)

                                                          cTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGCCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCAc                                                                                         <  2:560961/132‑1 (MQ=255)

                                                            gggCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGCCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTg                                                                    <  1:415485/151‑1 (MQ=255)

                                                             ggCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGCCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGa                                                                   <  2:641537/151‑1 (MQ=255)

                                                              gCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGCCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGAc                                                                  <  1:701236/151‑1 (MQ=255)

                                                                 ttACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGCCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACg                                                                 <  2:660124/149‑1 (MQ=255)

                                                                    cGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGCCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACa                                                            <  2:323036/151‑1 (MQ=255)

                                                                          gCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGCCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCtt                                                      <  2:97467/151‑1 (MQ=255)

                                                                                       ccGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGCCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTc                                         <  1:705985/151‑1 (MQ=255)

                                                                                               ggCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGCCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCa                                 <  1:457395/151‑1 (MQ=255)

                                                                                                gCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGCCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCag                                <  1:193103/151‑1 (MQ=255)

                                                                                                          gatgaGCTTCATCGTCTCCAGCGCCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAg                       <  1:112332/150‑1 (MQ=255)

                                                                                                                  tCATCGTCTCCAGCGCCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGAt               <  2:749138/150‑1 (MQ=255)

                                                                                                                           ccAGCGCCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCAt     <  2:614731/151‑1 (MQ=255)

                                                                                                                            cAGCGCCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATg    <  1:522140/151‑1 (MQ=255)

                                                                                                                              gcgcCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCt  <  2:35758/151‑1 (MQ=255)

                                                                                                                              gcgcCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCt  <  1:722593/151‑1 (MQ=255)

CCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCGACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTTGCTCAATCCGTTCGGCAAGCAGAGTCGCCAGATGTTGATAACGCGTCATGCT  >  NC_000913/4571301‑4571577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A