Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F50 I1 R1 157 93.7 2947622 98.1% 2891617 150.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,609,458 T→C R324R (CGT→CGC)  hyfG → hydrogenase 4, subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,609,4580TC100.0% 389.7 / NA 103R324R (CGT→CGC) hyfGhydrogenase 4, subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (64/39);  total (64/39)

Only 100 of 104 total aligned reads displayed.

GATTCTGCTGGAAGTCGAACGGCTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGTAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTAGAGATGCTGCTTGCTACGCCGAATATGGAACA  >  NC_000913/2609311‑2609604
                                                                                                                                                   |                                                                                                                                                  
gATTCTGCTGGAAGTCGAACGGCTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCaaa                                                                                                                                                 <  1:73716/151‑1 (MQ=255)
   tCTGCTGGAAGTCGAACGGCTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAAcc                                                                                                                                              >  2:1061764/1‑151 (MQ=255)
      gctgGAAGTCGAACGGCTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTa                                                                                                                                            <  1:829981/150‑1 (MQ=255)
       ctgGAAGTCGAACGGCTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACg                                                                                                                                          >  2:818617/1‑151 (MQ=255)
                gAACGGCTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATc                                                                                                                                 >  1:38854/1‑151 (MQ=255)
                gAACGGCTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATc                                                                                                                                 >  2:661861/1‑151 (MQ=255)
                  aCGGCTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTg                                                                                                                               <  1:1176670/151‑1 (MQ=255)
                    ggCTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGAt                                                                                                                             >  1:991834/1‑151 (MQ=255)
                     gCTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGAtt                                                                                                                            >  1:921274/1‑151 (MQ=255)
                       tACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATtgg                                                                                                                          >  1:689330/1‑151 (MQ=255)
                       tACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATtgg                                                                                                                          >  1:1411772/1‑151 (MQ=255)
                        acacAGTCATTTGCTTACCCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATtgg                                                                                                                          <  2:75530/150‑1 (MQ=255)
                         cacaGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATtggtg                                                                                                                        >  1:1220179/1‑151 (MQ=255)
                            aGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGgtggtg                                                                                                                     >  1:524751/1‑151 (MQ=255)
                                   tGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTcgccg                                                                                                              >  2:1111120/1‑151 (MQ=255)
                                   tGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTcgcc                                                                                                               <  2:644614/150‑1 (MQ=255)
                                    gCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTcgccgc                                                                                                             >  1:1026960/1‑151 (MQ=255)
                                    gCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTcgccg                                                                                                              <  2:879025/150‑1 (MQ=255)
                                         aCCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGatat                                                                                                        >  2:145171/1‑151 (MQ=255)
                                          ccTTCGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATAtt                                                                                                       >  1:40452/1‑151 (MQ=255)
                                            ttGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATtct                                                                                                     >  1:1374814/1‑151 (MQ=255)
                                            ttGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATtct                                                                                                     >  2:267403/1‑151 (MQ=255)
                                            ttGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATtct                                                                                                     >  1:1450502/1‑151 (MQ=255)
                                            ttGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATtct                                                                                                     >  1:239642/1‑151 (MQ=255)
                                            ttGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATtct                                                                                                     >  1:380079/1‑151 (MQ=255)
                                            ttGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATtc                                                                                                      >  2:1336672/1‑150 (MQ=255)
                                             tGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATtctc                                                                                                    >  1:116213/1‑151 (MQ=255)
                                             tGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATtc                                                                                                      <  2:1128836/149‑1 (MQ=255)
                                              ggCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCa                                                                                                   <  1:1014646/151‑1 (MQ=255)
                                               gCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCa                                                                                                   >  1:834024/1‑150 (MQ=255)
                                                           aTTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCt                                                                                      <  1:1152661/151‑1 (MQ=255)
                                                           aTTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCt                                                                                      <  1:827817/151‑1 (MQ=255)
                                                            tttCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTg                                                                                     >  1:46677/1‑151 (MQ=255)
                                                               cGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCa                                                                                   <  1:550424/150‑1 (MQ=255)
                                                               cGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCa                                                                                   >  2:1418055/1‑150 (MQ=255)
                                                                gTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCaa                                                                                  >  2:125535/1‑150 (MQ=255)
                                                                   ggTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACg                                                                               <  2:1337466/150‑1 (MQ=255)
                                                                   ggTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGc                                                                              >  2:1297502/1‑151 (MQ=255)
                                                                      tttGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTg                                                                            >  1:1082044/1‑150 (MQ=255)
                                                                      tttGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTg                                                                            >  2:333110/1‑150 (MQ=255)
                                                                      tttGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTg                                                                            >  1:837889/1‑150 (MQ=255)
                                                                        tGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGaa                                                                          >  2:1187913/1‑150 (MQ=255)
                                                                         gATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGaaa                                                                         <  1:426368/150‑1 (MQ=255)
                                                                            aCCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTgg                                                                     >  2:151751/1‑151 (MQ=255)
                                                                             ccGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGt                                                                    <  2:652124/151‑1 (MQ=255)
                                                                             ccGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGt                                                                    <  1:1257350/151‑1 (MQ=255)
                                                                             ccGGCTGTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGt                                                                    >  2:1309142/1‑151 (MQ=255)
                                                                               ggCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTgc                                                                  <  1:1353226/151‑1 (MQ=255)
                                                                               ggCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTgc                                                                  >  2:73026/1‑151 (MQ=255)
                                                                                gCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTgc                                                                  >  2:1416070/1‑150 (MQ=255)
                                                                                  tttATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGGAACTGGTGcgcg                                                               >  1:1123827/1‑151 (MQ=255)
                                                                                   ttATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGcgcg                                                               >  2:863231/1‑150 (MQ=255)
                                                                                      tGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGcgcg                                                               >  2:1218867/1‑147 (MQ=255)
                                                                                       gCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATg                                                          <  1:520663/151‑1 (MQ=255)
                                                                                        cAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATgc                                                         >  1:722485/1‑151 (MQ=255)
                                                                                        cAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATgc                                                         >  1:55644/1‑151 (MQ=255)
                                                                                         aaTTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATgcg                                                        >  1:927236/1‑151 (MQ=255)
                                                                                          aTTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATgcgc                                                       >  1:257235/1‑151 (MQ=255)
                                                                                          aTTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATgcgc                                                       >  1:285341/1‑151 (MQ=255)
                                                                                            ttttCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATgcgcgc                                                     >  2:817996/1‑151 (MQ=255)
                                                                                             tttCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGcc                                                    >  1:510558/1‑151 (MQ=255)
                                                                                             tttCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGcc                                                    >  1:535075/1‑151 (MQ=255)
                                                                                              ttCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTAAGGACTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCg                                                   >  1:847649/1‑151 (MQ=255)
                                                                                               tCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATgcgcgc                                                     >  2:1159672/1‑148 (MQ=255)
                                                                                               tCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCg                                                   >  2:373504/1‑150 (MQ=255)
                                                                                                ccGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGAc                                                 >  2:1101664/1‑151 (MQ=255)
                                                                                                ccGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGAc                                                 >  1:1158885/1‑151 (MQ=255)
                                                                                                ccGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGAc                                                 <  2:1295515/151‑1 (MQ=255)
                                                                                                ccGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGAc                                                 <  2:1026863/151‑1 (MQ=255)
                                                                                                 cgcgTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACg                                                >  2:1388025/1‑151 (MQ=255)
                                                                                                 cgcgTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACg                                                <  2:815529/151‑1 (MQ=255)
                                                                                                  gcgTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACgt                                               <  1:1194083/151‑1 (MQ=255)
                                                                                                     tGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCGAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTc                                            >  1:1064465/1‑151 (MQ=255)
                                                                                                     tGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTc                                            >  1:85409/1‑151 (MQ=255)
                                                                                                     tGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTc                                            >  1:442343/1‑151 (MQ=255)
                                                                                                      gCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTc                                            <  1:1224276/150‑1 (MQ=255)
                                                                                                      gCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCg                                           <  2:1217140/151‑1 (MQ=255)
                                                                                                      gCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCg                                           <  1:451629/151‑1 (MQ=255)
                                                                                                       cGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCgg                                          <  2:1091851/151‑1 (MQ=255)
                                                                                                       cGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCgg                                          <  1:1287706/151‑1 (MQ=255)
                                                                                                       cGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCgg                                          <  2:546766/151‑1 (MQ=255)
                                                                                                        gggAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCgg                                          >  2:447949/1‑150 (MQ=255)
                                                                                                           aaaaGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGc                                       <  1:1336779/150‑1 (MQ=255)
                                                                                                               gTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTa                                  <  1:1468381/151‑1 (MQ=255)
                                                                                                               gTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTa                                  <  2:641389/151‑1 (MQ=255)
                                                                                                                 cGATGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGt                                   >  2:982287/1‑148 (MQ=255)
                                                                                                                    tGACGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTag                                 >  1:1277576/1‑147 (MQ=255)
                                                                                                                       cGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTAGAGAtgct                          >  1:288971/1‑151 (MQ=255)
                                                                                                                       cGATGGCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTAGAGAtgct                          >  1:1396057/1‑151 (MQ=255)
                                                                                                                           ggCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTAGAGATGCtgctt                      <  2:842405/151‑1 (MQ=255)
                                                                                                                            gCGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTAGAGATGCtgcttg                     <  2:1181921/151‑1 (MQ=255)
                                                                                                                             cGGAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTAGAGATGCtgcttgc                    <  2:921187/151‑1 (MQ=255)
                                                                                                                              ggAATTGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTAGAGATGCtgcttgct                   >  1:934143/1‑151 (MQ=255)
                                                                                                                                   tGCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTAGAGATGCTGCTTGCTACGcc              >  2:582977/1‑151 (MQ=255)
                                                                                                                                   tGCTGATCAGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTAGAGATGCTTCTTGCTACGc               <  2:78672/150‑1 (MQ=255)
                                                                                                                                    gCTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTAGAGATGCTGCTTGCTACGCCg             <  2:1295231/151‑1 (MQ=255)
                                                                                                                                     cTGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTAGAGATGCTGCTTGCTACGCCg             >  1:1433760/1‑150 (MQ=255)
                                                                                                                                      tGATCGGGTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTAGAGATGCTGCTTGCTACGCCGa            >  2:861126/1‑150 (MQ=255)
                                                                                                                                           gggTCGCGCAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTAGAGATGCTGCTTGCTACGCCGAATATgg      <  2:363969/151‑1 (MQ=255)
                                                                                                                                                gcgcAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTAGAGATGCTGCTTGCTACGCCGAATATGGaaca  >  1:1294379/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GATTCTGCTGGAAGTCGAACGGCTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGTAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTAGAGATGCTGCTTGCTACGCCGAATATGGAACA  >  NC_000913/2609311‑2609604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A