Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F50 I1 R1 157 93.7 2947622 98.1% 2891617 150.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,515,565 A→G Y117H (TAC→CAC)  dam ← DNA adenine methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,515,5650AG95.5% 306.9 / 4.2 88Y117H (TAC→CAC) damDNA adenine methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (3/1);  new base G (45/39);  total (48/40)
Fisher's exact test for biased strand distribution p-value = 6.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.92e-01

GTAAGACTCACAATAGAAAAAGGCATTCTGCGCTTTTTCAGCGAAGTGATACAACTCTGCTTCCGGGAAATAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTAGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTAC  >  NC_000913/3515419‑3515708
                                                                                                                                                  |                                                                                                                                               
gTAAGACTCACAATAGAAAAAGGCATTCTGCGCTTTTTCAGCGAAGTGATACAACTCTGCTTCCGGGAAATAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCgg                                                                                                                                             <  1:135087/151‑1 (MQ=255)
    gACTCACAATAGAAAAAGGCATTCTGCGCTTTTTCAGCGAAGTGATACAACTCTGCTTCCGGGAAATAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCa                                                                                                                                         <  1:50621/151‑1 (MQ=255)
     aCTCACAATAGAAAAAGGCATTCTGCGCTTTTTCAGCGAAGTGATACAACTCTGCTTCCGGGAAATAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCa                                                                                                                                         >  1:445675/1‑150 (MQ=255)
      cTCACAATAGAAAAAGGCATTCTGCGCTTTTTCAGCGAAGTGATACAACTCTGCTTCCGGGAAATAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGATCaa                                                                                                                                        >  1:953144/1‑150 (MQ=255)
        cacaATAGAAAAAGGCATTCTGCGCTTTTTCAGCGAAGTGATACAACTCTGCTTCCGGGAAATAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCaaa                                                                                                                                       <  2:1092680/149‑1 (MQ=255)
        cacaATAGAAAAAGGCATTCTGCGCTTTTTCAGCGAAGTGATACAACTCTGCTTCCGGGAAATAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAat                                                                                                                                      <  1:745671/150‑1 (MQ=255)
          caATAGAAAAAGGCATTCTGCGCTTTTTCAGCGAAGTGATACAACTCTGCTTCCGGGAAATAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAata                                                                                                                                     >  2:730518/1‑149 (MQ=255)
              aGAAAAAGGCATTCTGCGCTTTTTCAGCGAAGTGATACAACTCTGCTTCCGGGAAATAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATaaaa                                                                                                                                >  1:1032295/1‑150 (MQ=255)
                aaaaaGGCATTCTGCGCTTTTTCAGCGAAGTGATACAACTCTGCTTCCGGGAAATAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATaaaaa                                                                                                                               >  1:1371472/1‑149 (MQ=255)
                aaaaaGGCATTCTGCGCTTTTTCAGCGAAGTGATACAACTCTGCTTCCGGGAAATAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTAGCGGTTCAAATATAAAAAc                                                                                                                              >  2:622470/1‑150 (MQ=255)
                 aaaaGGCATTCTGCGCTTTTTCAGCGAAGTGATACAACTCTGCTTCCGGGAAATAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAAc                                                                                                                              >  1:350941/1‑149 (MQ=255)
                  aaaGGCATTCTGCGCTTTTTCAGCGAAGTGATACAACTCTGCTTCCGGGAAATAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATaaaaaaa                                                                                                                             >  2:1330470/1‑147 (MQ=255)
                  aaaGGCATTCTGCGCTTTTTCAGCGAAGTGATACAACTCTGCTTCCGGGAAATAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGt                                                                                                                           <  1:394914/151‑1 (MQ=255)
                   aaGGCATTCTGCGCTTTTTCAGCGAAGTGATACACCTCTGCTTCCGGGAAATAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTa                                                                                                                          <  1:222272/151‑1 (MQ=255)
                        aTTCTGCGCTTTTTCAGCGAAGTGATACAACTCTGCTTCCGGGAAATAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGcc                                                                                                                     >  2:1207057/1‑151 (MQ=255)
                         ttCTGCGCTTTTTCAGCGAAGTGATACAACTCTGCTTCCGGGAAATAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTAcccgcc                                                                                                                    >  1:734175/1‑147 (MQ=255)
                             gcgcTTTTTCAGCGAAGTGATACAACTCTGCTTCCGGGAAATAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCg                                                                                                                   <  2:820246/148‑1 (MQ=255)
                             gcgcTTTTTCAGCGAAGTGATACAACTCTGCTTCCGGGAAATAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGa                                                                                                                  <  2:1419893/149‑1 (MQ=255)
                              cgcTTTTTCAGCGAAGTGATACAACTCTGCTTCCGGGAAATAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAAAAGTACCGCCCGacgg                                                                                                               >  2:1405957/1‑151 (MQ=255)
                                cTTTTTCAGCGAAGTGATACAACTCTGCTTCCGGGAAATAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGacgg                                                                                                               >  2:273765/1‑149 (MQ=255)
                                  ttttCAGCGAAGTGATACAACTCTGCTTCCGGGAAATAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCCAATATAACAACAGGAcctgccggtgg                                                                                                               >  2:577615/1‑138 (MQ=255)
                                   tttCAGCGAAGTGATACAACTCTGCTTCCGGGAAATAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGacggaacg                                                                                                           <  1:1257738/150‑1 (MQ=255)
                                   tttCAGCGAAGTGATACAACTCTGCTTCCGGGAAATAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGacggaacg                                                                                                           <  2:1170173/150‑1 (MQ=255)
                                    ttCAGCGAAGTGATACAACTCTGCTTCCGGGAAATAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGAACCGCCCGacggaacgg                                                                                                          >  1:139993/1‑150 (MQ=255)
                                     tCAGCGAAGTGATACAACTCTGCTTCCGGGAAATAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCCacgggacgga                                                                                                         >  1:1294915/1‑150 (MQ=255)
                                       aGCGAAGTGATACAACTCTGCTTCCGGGAAATAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCgacgggacgg                                                                                                          >  1:1157616/1‑147 (MQ=255)
                                          gAAGTGATACAACTCTGCTTCCGGGAAATAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTgg                                                                                                   >  1:1397408/1‑151 (MQ=255)
                                          gAAGTGATACAACTCTGCTTCCGGGAAATAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTgg                                                                                                   >  1:130290/1‑151 (MQ=255)
                                             gTGATACAACTCTGCTTCCGGGAAATAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACACTACCGCCCGACGGAACGGATTCTGGCtt                                                                                                >  2:1220896/1‑151 (MQ=255)
                                             gTGATACAACTCTGCTTCCGGGAAATAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAAAAGTACCGCCCGACGGAACGGATCCTGGCtt                                                                                                >  1:326763/1‑151 (MQ=255)
                                               gATACAACTCTGCTTCCGGGAAATAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTGGCttt                                                                                               <  2:1091918/150‑1 (MQ=255)
                                                aTACAACTCTGCTTCCGGGAAATAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCCAAAATAAAAACAGTACCGCCCGACGGAACGGGTCCCGGCtttt                                                                                              >  2:72811/1‑150 (MQ=255)
                                                  aCAACTCTGCTTCCGGGAAATAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTGGCTTttgt                                                                                            <  2:207653/150‑1 (MQ=255)
                                                   cAACTCTGCTTCCGGGAAATAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTGGCTTttgttg                                                                                          <  1:191696/151‑1 (MQ=255)
                                                   cAACTCTGCTTCCGGGAAATAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTGGCTTttgtt                                                                                           >  1:325047/1‑150 (MQ=255)
                                                       tctGCTTCCGGGAAATAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGGTCAAATATAAAAAAAGTACCGCCCGACGGAACGGAACCTGGCTTTTGTTGaa                                                                                        >  2:1026346/1‑149 (MQ=255)
                                                        ctGCTTCCGGGAAATAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAATACCGCCCGACGGAAAGGATCCTGGCTTTTGTTGAActc                                                                                     >  2:257063/1‑151 (MQ=255)
                                                              ccGGGAAATAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTGGCTTTTGTTTAACTCTTcgc                                                                                >  2:591464/1‑150 (MQ=255)
                                                              ccGGGAAATAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTcgcg                                                                               <  1:793703/151‑1 (MQ=255)
                                                                gggAAATAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCCGGGTTTTGTTGAACTCttttcgg                                                                              >  2:1455498/1‑145 (MQ=255)
                                                                gggAAATAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAAAGGATCCTGGCTTTTGTTGAACTCTTCGCgg                                                                              >  2:1079709/1‑150 (MQ=255)
                                                                   aaaTAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGGACCGATCCTGGCTTTTTTTTGAATCTTTGCGGaa                                                                            >  1:981544/1‑149 (MQ=255)
                                                                   aaaTAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGaa                                                                            >  1:695729/1‑149 (MQ=255)
                                                                   aaaTAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACt                                                                          <  2:1151931/151‑1 (MQ=255)
                                                                    aaTAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCCGGCTTTTTTTGAACTCTTCGCGGGACTg                                                                         >  1:1130314/1‑151 (MQ=255)
                                                                      tAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTAACGCCCGACGGAACCGATCCCGGCTTTTGTTGAACTCTTCGCGGAACt                                                                          >  2:1360147/1‑148 (MQ=255)
                                                                       aGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATa                                                                      <  2:560693/151‑1 (MQ=255)
                                                                        gggTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAAAAGTACCGCCCGACGGGACGGGTCCTGGCTTTTTTTGAACTCTTCGCGGaa                                                                            >  2:794450/1‑144 (MQ=255)
                                                                          gTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAAAAATACCGCCCGGCGGAACGGGGCCTGGCTTTTGTTTAACTCTTCGCCGGACTg                                                                         >  2:690161/1‑145 (MQ=255)
                                                                             ttttGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTaaa                                                                 <  2:741665/150‑1 (MQ=255)
                                                                             ttttGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTaaa                                                                 >  1:590574/1‑150 (MQ=255)
                                                                              tttGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAAc                                                                <  1:537987/150‑1 (MQ=255)
                                                                                  tAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAAAATAAAAACAGTACCGCCCGGCGGAACGGGTCCTGGCTTTTGTTGAAATCTTCGCGGAAATGATAGCAAAACTCg                                                            >  2:464990/1‑150 (MQ=255)
                                                                                   aGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCgg                                                           >  1:1188689/1‑150 (MQ=255)
                                                                                   aGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTAGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCgg                                                           <  1:622543/150‑1 (MQ=255)
                                                                                    gCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCg                                                            >  2:335098/1‑148 (MQ=255)
                                                                                       gCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCaa                                                      <  2:1150453/151‑1 (MQ=255)
                                                                                        ccGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAAt                                                     <  1:1063214/151‑1 (MQ=255)
                                                                                        ccGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTAACCCCCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGAGAGTAAACCTCGGCGCa                                                       >  1:607604/1‑149 (MQ=255)
                                                                                         cGAACTGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTAGCGGTTAAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAAtt                                                    >  1:57672/1‑151 (MQ=255)
                                                                                         cGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAAtt                                                    <  1:1330578/151‑1 (MQ=255)
                                                                                         cGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGGAAACCTCGGCGCAAtt                                                    >  1:788674/1‑151 (MQ=255)
                                                                                               gCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTAGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTc                                              >  1:779823/1‑151 (MQ=255)
                                                                                                  cGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCggg                                           <  2:267228/151‑1 (MQ=255)
                                                                                                      aaaCTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGaaca                                       <  2:312888/151‑1 (MQ=255)
                                                                                                       aaCTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGaacaa                                      <  1:377171/151‑1 (MQ=255)
                                                                                                           cACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGTACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAACCCTCGGCGCAATTTGTTTCGGGAACAAACAg                                  <  2:1204396/151‑1 (MQ=255)
                                                                                                           cACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGaacaaaca                                   <  2:716751/150‑1 (MQ=255)
                                                                                                             ccGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCt                                <  1:1207154/151‑1 (MQ=255)
                                                                                                               gcgcAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTcg                              <  1:1406065/151‑1 (MQ=255)
                                                                                                                 gcAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTcgc                             <  1:1164907/150‑1 (MQ=255)
                                                                                                                 gcAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTcgc                             <  1:565770/150‑1 (MQ=255)
                                                                                                                  cAGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGt                           <  2:326723/151‑1 (MQ=255)
                                                                                                                   aGATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTg                          <  2:735840/151‑1 (MQ=255)
                                                                                                                    gATTGTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTAACGCCCCACGGAACGGATCCTGGCTTTTGTTTAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTg                          >  2:216593/1‑150 (MQ=255)
                                                                                                                      ttGTAACGACACAGGCCGTTGTAACCGTGGCGGTTAAAATATTAAAACAGAACCGCCCGACGGGACGGATTCTGGCTTTTGTTGAAATCTTCGCGGAAATGATAGGAAAACTCGGCGCAATTTGTTTTGGGAACAAAAAGCTCGCGTGCgg                       >  2:1343950/1‑151 (MQ=255)
                                                                                                                        gTAACGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGcc                     <  2:445622/151‑1 (MQ=255)
                                                                                                                          aaCGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGcc                     >  2:323624/1‑149 (MQ=255)
                                                                                                                           aCGACACAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTgt                  <  2:1232089/151‑1 (MQ=255)
                                                                                                                              acacAGGCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTgtac                >  1:1231712/1‑150 (MQ=255)
                                                                                                                                    gCCGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACtc          >  2:607331/1‑150 (MQ=255)
                                                                                                                                      cGTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACtcatc       <  1:193259/151‑1 (MQ=255)
                                                                                                                                       gTTGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACtcatca      <  2:1375620/151‑1 (MQ=255)
                                                                                                                                         tGTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAg     <  1:384800/150‑1 (MQ=255)
                                                                                                                                          gTAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTa   <  2:130272/151‑1 (MQ=255)
                                                                                                                                           tAACCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTGGCCTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTAc  >  1:986135/1‑151 (MQ=255)
                                                                                                                                            aaCCGTGGCGGTTCCAATATAAAAACAGTACCGCCCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAAATGATAGTAAACCTCGGCGCAATTTGTTTTGGGAACAAACAGCCCGCGTGCGGCCCGTACGTACTCATCAGTAc  >  2:447201/1‑150 (MQ=255)
                                                                                                                                            aaCCGTGGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTAc  <  2:136817/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
GTAAGACTCACAATAGAAAAAGGCATTCTGCGCTTTTTCAGCGAAGTGATACAACTCTGCTTCCGGGAAATAGGGTTTTTTGTAGCGGCCGAACGGCACGTTAAACTCACCGCGCAGATTGTAACGACACAGGCCGTTGTAACCGTAGCGGTTCAAATATAAAAACAGTACCGCCCGACGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTAC  >  NC_000913/3515419‑3515708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A