Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F50 I1 R1 157 93.7 2947622 98.1% 2891617 150.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,401,589 C→T D306D (GAC→GAT)  hflX → GTPase, stimulated by 50S subunit binding; Mn(2+) homeostasis regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,401,5890CT97.1% 378.5 / ‑2.1 104D306D (GAC→GAT) hflXGTPase, stimulated by 50S subunit binding; Mn(2+) homeostasis regulator
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base T (45/56);  total (47/57)
Fisher's exact test for biased strand distribution p-value = 5.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 104 total aligned reads displayed.

GCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAG  >  NC_000913/4401441‑4401736
                                                                                                                                                    |                                                                                                                                                   
gCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGc                                                                                                                                                   <  1:159738/151‑1 (MQ=255)
    ccTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCAc                                                                                                                                               <  1:21170/151‑1 (MQ=255)
        ccGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACgaga                                                                                                                                           <  1:897845/151‑1 (MQ=255)
         cGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGAt                                                                                                                                          <  2:784228/151‑1 (MQ=255)
          gCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATc                                                                                                                                         <  1:1443885/151‑1 (MQ=255)
           cACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGGGAATACGGTTCTTGAAGAGAACGATGCTCACGAGATcc                                                                                                                                        >  2:1001042/1‑151 (MQ=255)
            aCGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATccc                                                                                                                                       >  1:658124/1‑151 (MQ=255)
              gATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCa                                                                                                                                      >  2:574698/1‑150 (MQ=255)
                  tggtggCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAAccc                                                                                                                                  >  1:1056851/1‑150 (MQ=255)
                   ggtggCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAAcc                                                                                                                                   <  1:1354473/148‑1 (MQ=255)
                   ggtggCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAAc                                                                                                                                    <  1:307553/147‑1 (MQ=255)
                   ggtggCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAAc                                                                                                                                    <  2:705757/147‑1 (MQ=255)
                      ggaggcATTTCCAGCCACGTTACAAGAGCCTCTTCTAGCCACCTTACTGCTGCACGTCATTTATGCGGCGGATGTGCGTGTACAAGAAAACGTCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCctgctg                                                                                                                             <  1:1043110/148‑1 (MQ=255)
                       gcggcATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTgg                                                                                                                            >  1:980131/1‑151 (MQ=255)
                          gcATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGtga                                                                                                                         <  1:1338736/151‑1 (MQ=255)
                          gcATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGt                                                                                                                           <  1:38269/149‑1 (MQ=255)
                          gcATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAAAACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCctgctg                                                                                                                             >  1:667545/1‑147 (MQ=255)
                              ttAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGtgatga                                                                                                                      <  1:892207/150‑1 (MQ=255)
                              ttAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGtgatga                                                                                                                      <  2:95452/150‑1 (MQ=255)
                                 aaGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACaa                                                                                                                  <  2:932272/151‑1 (MQ=255)
                                  aaccACGTTACAAGAGACGCGGCAAGCCACATTACTGGTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGTTTATGAACAAg                                                                                                                 <  2:826524/149‑1 (MQ=255)
                                    ccACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACa                                                                                                                   >  1:175498/1‑147 (MQ=255)
                                    ccACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGAAGGGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCCGGTGATGAACaa                                                                                                                  >  2:267185/1‑148 (MQ=255)
                                     cACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATc                                                                                                              <  2:224549/151‑1 (MQ=255)
                                      aCGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAAc                                                                                                                    >  2:33714/1‑144 (MQ=255)
                                       cGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGa                                                                                                            <  1:760491/151‑1 (MQ=255)
                                         ttACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGa                                                                                                            >  2:563806/1‑149 (MQ=255)
                                             aaGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCt                                                                                                      >  1:143967/1‑151 (MQ=255)
                                             aaGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGGGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCt                                                                                                      >  1:737792/1‑151 (MQ=255)
                                              agagACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTg                                                                                                     >  2:423136/1‑151 (MQ=255)
                                                 gaCGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGaa                                                                                                  <  2:1197797/151‑1 (MQ=255)
                                                 gaCGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGaa                                                                                                  <  2:1149844/151‑1 (MQ=255)
                                                 gaCGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGaa                                                                                                  <  1:574764/151‑1 (MQ=255)
                                                 gaCGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGa                                                                                                   >  2:947789/1‑150 (MQ=255)
                                                  aCGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAg                                                                                                 <  2:369225/151‑1 (MQ=255)
                                                     cgGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGAtt                                                                                              >  1:924187/1‑151 (MQ=255)
                                                          aGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCg                                                                                           >  1:804515/1‑149 (MQ=255)
                                                           gCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAAc                                                                                        >  1:1436542/1‑151 (MQ=255)
                                                             cacaTTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAAAAAGATCGATATGCTGGAAGATTTCGAACcg                                                                                      >  2:737235/1‑151 (MQ=255)
                                                             cacaTTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATAAGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAAcc                                                                                       >  1:1432502/1‑150 (MQ=255)
                                                              acaTTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACcgc                                                                                     >  1:578648/1‑151 (MQ=255)
                                                                aTTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGGGATCGGTGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGt                                                                                   >  1:243580/1‑151 (MQ=255)
                                                                   aCTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTAtt                                                                                <  2:4725/151‑1 (MQ=255)
                                                                   aCTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTAtt                                                                                <  1:21473/151‑1 (MQ=255)
                                                                      gctgcACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTg                                                                               <  1:1270199/149‑1 (MQ=255)
                                                                      gctgcACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTg                                                                               <  2:1008331/149‑1 (MQ=255)
                                                                      gctgcACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGa                                                                              <  1:861130/150‑1 (MQ=255)
                                                                      gctgcACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGa                                                                              <  2:1303888/150‑1 (MQ=255)
                                                                       ctgcACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTg                                                                               <  2:685885/148‑1 (MQ=255)
                                                                       ctgcACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGAt                                                                             >  1:747680/1‑150 (MQ=255)
                                                                       ctgcACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATc                                                                            >  1:662775/1‑151 (MQ=255)
                                                                         gcACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATc                                                                            <  2:83880/149‑1 (MQ=255)
                                                                         gcACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATTTCAACCCTGCTGGTGATGAACAAAATCGATATGCTGGAAGAATTCGAACCGCGTATTGATCgg                                                                          >  2:1018105/1‑151 (MQ=255)
                                                                          cACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCggg                                                                         <  2:880650/151‑1 (MQ=255)
                                                                           aCGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGa                                                                        <  1:1236432/151‑1 (MQ=255)
                                                                           aCGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGa                                                                        <  2:780951/151‑1 (MQ=255)
                                                                              tCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACg                                                                      >  2:1234567/1‑150 (MQ=255)
                                                                               cATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGAc                                                                       >  1:1016485/1‑148 (MQ=255)
                                                                               cATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGa                                                                     >  2:947701/1‑150 (MQ=255)
                                                                                    aTGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGaa                                                               <  2:1017512/151‑1 (MQ=255)
                                                                                      gcggcgGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAAc                                                              <  1:1216331/150‑1 (MQ=255)
                                                                                      gcggcgGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACa                                                             <  1:765807/151‑1 (MQ=255)
                                                                                       ccgcggATGTGCGTGTACAATAAAACATCGAAGCGGTGAATCCGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACa                                                             <  1:470347/148‑1 (MQ=255)
                                                                                         gcggATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAaac                                                          >  1:1066179/1‑151 (MQ=255)
                                                                                           ggATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAaaccg                                                        <  1:1147272/151‑1 (MQ=255)
                                                                                           ggATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAaaccg                                                        >  1:650589/1‑151 (MQ=255)
                                                                                           ggATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAaaccg                                                        <  2:525379/151‑1 (MQ=255)
                                                                                            gATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAaaccga                                                       <  2:851248/151‑1 (MQ=255)
                                                                                             aTGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAaacctaa                                                      <  2:1275989/151‑1 (MQ=255)
                                                                                               gtgCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAaaccgaacc                                                    >  2:727955/1‑151 (MQ=255)
                                                                                                  cGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCgtg                                                 <  2:1157933/151‑1 (MQ=255)
                                                                                                  cGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCgtg                                                 <  1:727188/151‑1 (MQ=255)
                                                                                                   gtgtACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCgtgt                                                <  1:1025401/151‑1 (MQ=255)
                                                                                                      tACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTg                                             >  2:848303/1‑151 (MQ=255)
                                                                                                      tACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTg                                             >  2:980456/1‑151 (MQ=255)
                                                                                                         aaGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCt                                          >  1:1338796/1‑151 (MQ=255)
                                                                                                              aaCATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCg                                     <  2:1405458/151‑1 (MQ=255)
                                                                                                               aCATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGc                                    <  1:1425050/151‑1 (MQ=255)
                                                                                                               aCATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGc                                    >  1:1201571/1‑151 (MQ=255)
                                                                                                                   cGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAg                                <  2:2409/151‑1 (MQ=255)
                                                                                                                   cGAAGCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAg                                <  2:609034/151‑1 (MQ=255)
                                                                                                                       gCGGTGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCg                            <  1:431846/151‑1 (MQ=255)
                                                                                                                           tGAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGc                        >  2:61963/1‑151 (MQ=255)
                                                                                                                            gAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGc                        <  1:63843/150‑1 (MQ=255)
                                                                                                                            gAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGc                        <  1:423192/150‑1 (MQ=255)
                                                                                                                            gAATACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCg                       <  2:437467/151‑1 (MQ=255)
                                                                                                                             aaTACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCgg                      >  2:92165/1‑151 (MQ=255)
                                                                                                                             aaTACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCgg                      >  1:992451/1‑151 (MQ=255)
                                                                                                                              aTACGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCggg                     >  2:1227760/1‑151 (MQ=255)
                                                                                                                                aCGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCgggg                    >  1:1208327/1‑150 (MQ=255)
                                                                                                                                 cGGTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGAt                  >  1:369681/1‑151 (MQ=255)
                                                                                                                                  ggTTCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGAt                  >  2:233056/1‑150 (MQ=255)
                                                                                                                                     tCTTGAAGAGATCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATAcc               <  1:796090/150‑1 (MQ=255)
                                                                                                                                            gagaTCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCttt       <  1:1001135/151‑1 (MQ=255)
                                                                                                                                            gagaTCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCttt       >  2:595688/1‑151 (MQ=255)
                                                                                                                                            gagaTCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCttt       <  2:1056755/151‑1 (MQ=255)
                                                                                                                                              gaTCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGACCCGCGTATTGATCGGGACGAAGAGAACAAACCGACCCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCttttt     <  2:1285034/151‑1 (MQ=255)
                                                                                                                                               aTCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTc    >  2:512138/1‑151 (MQ=255)
                                                                                                                                                tCGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTc    >  1:1084198/1‑150 (MQ=255)
                                                                                                                                                 cGATGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAg  <  2:678634/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCCACCTGCCGCACGATCTGGTGGCGGCATTTAAAGCCACGTTACAAGAGACGCGGCAAGCCACATTACTGCTGCACGTCATTGATGCGGCGGATGTGCGTGTACAAGAAAACATCGAAGCGGTGAATACGGTTCTTGAAGAGATCGACGCTCACGAGATCCCAACCCTGCTGGTGATGAACAAGATCGATATGCTGGAAGATTTCGAACCGCGTATTGATCGGGACGAAGAGAACAAACCGAACCGTGTCTGGCTTTCCGCACAGACCGGAGCGGGGATACCACAGCTTTTTCAG  >  NC_000913/4401441‑4401736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A