Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,124,108	(G)_6→7	intergenic (‑54/+10)	grxB ← / ← mdtH	glutaredoxin 2 (Grx2)/multidrug resistance efflux transporter conferring overexpression resistance to norfloxacin and enoxacin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,124,102	1	.	G	100.0%	127.7 / NA	35	intergenic (‑48/+16)	grxB/mdtH	glutaredoxin 2 (Grx2)/multidrug resistance efflux transporter conferring overexpression resistance to norfloxacin and enoxacin
Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (16/19); total (16/19)

CGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCA‑GGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTAAACTGCCAACCCAGCGCAAGGAAAGTGAAGATGCCAATAATGCCCAGCATCATCCACGGAAGCTCTGGCTGGTGCGCCGATTT  >  NC_000913/1123955‑1124246

cgtTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCA‑ggg                                                                                                                                               >  1:167642/1‑151 (MQ=255)

 gtTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCA‑gggg                                                                                                                                              >  2:577854/1‑151 (MQ=255)

 gtTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCA‑gggg                                                                                                                                              >  2:356175/1‑151 (MQ=255)

 gtTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCA‑gggg                                                                                                                                              >  1:679212/1‑151 (MQ=255)

            cATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCaa                                                                                                                                    <  2:122183/151‑1 (MQ=255)

                    tCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCg                                                                                                                            >  1:322027/1‑151 (MQ=255)

                        cGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCAGTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTcgc                                                                                                                        >  1:552360/1‑151 (MQ=255)

                         ggggATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGGTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTcgcg                                                                                                                       >  2:8982/1‑151 (MQ=255)

                         ggggATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTcgcg                                                                                                                       <  2:323741/151‑1 (MQ=255)

                                 ttttCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGc                                                                                                                <  2:18050/150‑1 (MQ=255)

                                  tttCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCa                                                                                                               <  2:160596/150‑1 (MQ=255)

                                   ttCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCaa                                                                                                              <  1:154363/150‑1 (MQ=255)

                                       ggCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAacgac                                                                                                         <  2:664786/151‑1 (MQ=255)

                                        gCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAacgacg                                                                                                        >  1:203895/1‑151 (MQ=255)

                                              aaTCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACgcgcggc                                                                                                  <  1:589977/151‑1 (MQ=255)

                                                  aTGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGt                                                                                              <  2:646736/151‑1 (MQ=255)

                                                      gggCTTTGAAGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCt                                                                                          >  1:200497/1‑151 (MQ=255)

                                                           ttGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCt                                                                                      >  2:72169/1‑150 (MQ=255)

                                                            tGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTa                                                                                     >  1:65488/1‑150 (MQ=255)

                                                             gAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTaa                                                                                    <  2:481998/150‑1 (MQ=255)

                                                              aGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTAAAc                                                                                  <  2:119641/151‑1 (MQ=255)

                                                                   gTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTAAACTGCCa                                                                             <  1:378844/151‑1 (MQ=255)

                                                                        ggCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTAAACTGCCAACCCa                                                                        <  1:647033/151‑1 (MQ=255)

                                                                          cAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTAAACTGCCAACCCAgc                                                                      <  2:492022/151‑1 (MQ=255)

                                                                             tGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTAAACTGCCAACCCAgcgc                                                                    >  1:594213/1‑150 (MQ=255)

                                                                                tCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTAAACTGCCAACCCAGCGCAAgg                                                                >  2:323394/1‑151 (MQ=255)

                                                                                       tGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTAAACTGCCAACCCAGCGCAAGGAAAGTg                                                          >  2:134623/1‑150 (MQ=255)

                                                                                        gTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTAAACTGCCAACCCAGCGCAAGGAAAGTGaa                                                        <  2:395492/151‑1 (MQ=255)

                                                                                            aGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTAAACTGCCAACCCAGCGCAAGGAAAGTGAAGAt                                                     <  2:560376/150‑1 (MQ=255)

                                                                                               ttCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTAAACTGCCAACCCAGCGCAAGGAAAGTGAAGATGcc                                                  >  2:252554/1‑150 (MQ=255)

                                                                                                  aCGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTAAACTGCCAACCCAGCGCAAGGAAAGTGAAGATGCCaata                                              <  2:203890/151‑1 (MQ=255)

                                                                                                      gACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTAAACTGCCAACCCAGCGCAAGGAAAGTGAAGATGCCAATAATg                                           <  1:446757/150‑1 (MQ=255)

                                                                                                      gACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTAAACTGCCAACCCAGCGCAAGGAAAGTGAAGATGCCAATAATGc                                          >  1:188782/1‑151 (MQ=255)

                                                                                                                tttGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTAAACTGCCAACCCAGCGCAAGGAAAGTGAAGATGCCAATAATGCCCAGcatcat                                >  2:481230/1‑151 (MQ=255)

                                                                                                                       gTCAGTTCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTAAACTGCCAACCCAGCGCAAGGAAAGTGAAGATGCCAATAATGCCCAGCATCATCCACgg                          <  2:104522/150‑1 (MQ=255)

                                                                                                                            ttCACTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTAAACTGCCAACCCAGCGCAAGGAAAGTGAAGATGCCAATAATGCCCAGCATCATCCACGGAAGctc                    >  1:225945/1‑151 (MQ=255)

                                                                                                                               aCTTTCAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTAAACTGCCAACCCAGCGCAAGGAAAGTGAAGATGCCAATAATGCCCAGCATCATCCACGGAAGCTctgg                 <  2:4084/151‑1 (MQ=255)

                                                                                                                                    cAGTATGAAGGAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTAAACTGCCAACCCAGCGCAAGGAAAGTGAAGATGCCAATAATGCCCAGCATCATCCACGGAAGCTctggctg              >  2:98383/1‑149 (MQ=255)

                                                                                                                                              gAGTCAGGGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTAAACTGCCAACCCAGCGCAAGGAAAGTGAAGATGCCAATAATGCCCAGCATCATCCACGGAAGCTCTGGCTGGTGCGCCGAttt  >  2:338102/1‑151 (MQ=255)

CGTTGAGCAGAACATGTAATTCGACGGGGATATTTTTCAGGCCGAAAATCATGCGGGCTTTGAGGCAGTAAGGGCAGTGATCGTAAATGTATAGCTTCACGTGACTCCTCCATTTGGCTGTCAGTTCACTTTCAGTATGAAGGAGTCA‑GGGGGGAGTATCAAATCAGGCGTCGCGTTCAAGCAAACGACGCGCGGCGCGTTTCTGGCTAAACTGCCAACCCAGCGCAAGGAAAGTGAAGATGCCAATAATGCCCAGCATCATCCACGGAAGCTCTGGCTGGTGCGCCGATTT  >  NC_000913/1123955‑1124246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A