Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F50 I2 R1 209 44.2 1360995 98.7% 1343302 150.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,172,139 A→G I609T (ATT→ACT)  mfd ← transcription‑repair coupling factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,172,1390AG100.0% 149.5 / NA 41I609T (ATT→ACT) mfdtranscription‑repair coupling factor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/24);  total (17/24)

AGGCACCAGCACCGCCACCTGCTTGTGGTTATCTACTGCCAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAACGCGAAGCCCTCTTTGGCGGCGCGTTGCGCGTAGATATCCAGCAATTCCGCCGCCACATCACGCACTT  >  NC_000913/1171994‑1172286
                                                                                                                                                 |                                                                                                                                                   
aGGCACCAGCACCGCCACCTGCTTGTGGTTATCTACTGCCAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAGTGGcc                                                                                                                                                >  2:261568/1‑151 (MQ=255)
aGGCACCAGCACCGCCACCTGCTTGTGGTTATCTACTGCCAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAGTGGcc                                                                                                                                                >  1:71826/1‑151 (MQ=255)
   cACCAGCACCGCCACCTGCTTGTGGTTATCTACTGCCAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAGTGGCCTgc                                                                                                                                             <  1:124761/151‑1 (MQ=255)
    aCCAGCACCGCCACCTGCTTGTGGTTATCTACTGCCAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAGTGGCCTgcg                                                                                                                                            <  2:412291/151‑1 (MQ=255)
       aGCACCGCCACCTGCTTGTGGTTATCTACTGCCAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAGTGGCCTGCGCCt                                                                                                                                         >  1:309886/1‑151 (MQ=255)
        gCACCGCCACCTGCTTGTGGTTATCTACTGCCAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAGTGGCCTGCGCCTg                                                                                                                                        <  2:243087/151‑1 (MQ=255)
          aCCGCCACCTGCTTGTGGTTATCTACTGCCAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAGTGGCCTGCGCCTg                                                                                                                                        <  2:79617/149‑1 (MQ=255)
           ccGCCACCTGCTTGTGGTTATCTACTGCCAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAGTGGCCTGCGCCTGAt                                                                                                                                      <  2:372444/150‑1 (MQ=255)
                      ttGTGGTTATCTACTGCCAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAGTGGCCTGCGCCTGATCCGGCGTGGttt                                                                                                                          >  1:677023/1‑151 (MQ=255)
                                  aCTGCCAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAGTGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGaa                                                                                                                <  1:466506/149‑1 (MQ=255)
                                  aCTGCCAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAGTGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGaa                                                                                                                <  1:533056/149‑1 (MQ=255)
                                         ggAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAGTGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCa                                                                                                       <  1:634519/151‑1 (MQ=255)
                                         ggAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAGTGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCa                                                                                                       <  2:270453/151‑1 (MQ=255)
                                                     gcATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAGTGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATa                                                                                           <  2:287785/151‑1 (MQ=255)
                                                      cATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAGTGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATAc                                                                                          >  2:255367/1‑151 (MQ=255)
                                                           ccACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAGTGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTc                                                                                     >  1:574764/1‑151 (MQ=255)
                                                               ttCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAGTGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACg                                                                                  >  1:658789/1‑150 (MQ=255)
                                                                   gTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAGTGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGAt                                                                                <  2:556000/148‑1 (MQ=255)
                                                                   gTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAGTGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGAt                                                                                <  1:294330/148‑1 (MQ=255)
                                                                   gTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAGTGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCg                                                                              <  2:295481/150‑1 (MQ=255)
                                                                       tACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAGTGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGttt                                                                         >  1:203165/1‑151 (MQ=255)
                                                                         ccAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAGTGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTa                                                                        >  2:167063/1‑150 (MQ=255)
                                                                             aGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAGTGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAAcgc                                                                   <  1:478226/151‑1 (MQ=255)
                                                                             aGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAGTGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAAcgc                                                                   <  1:422393/151‑1 (MQ=255)
                                                                                 aaCATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAGTGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAACGCGa                                                                 >  1:666220/1‑149 (MQ=255)
                                                                                   cATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAGTGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAACGCGAAGc                                                              <  2:495212/150‑1 (MQ=255)
                                                                                      cgccgcACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAGTGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAACGCGAAGCCctc                                                          >  2:22517/1‑151 (MQ=255)
                                                                                      cgccgcACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAGTGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAACGCGAAGCCct                                                           <  2:266299/150‑1 (MQ=255)
                                                                                      cgccgaACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAGTGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAACGCGAAGCCct                                                           >  2:340089/1‑150 (MQ=255)
                                                                                         cgcACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAGTGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAACGCGAAGCCCTCttt                                                       <  2:117538/151‑1 (MQ=255)
                                                                                          gcACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAGTGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAACGCGAAGCCCTCTTTg                                                      >  2:604517/1‑151 (MQ=255)
                                                                                                   aCGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAGTGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAACGCGAAGCCCTCTTTGGCGGCGCGt                                             <  1:595586/151‑1 (MQ=255)
                                                                                                       tCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAGTGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAACGCGAAGCCCTCTTTGGCGGCGCGTTgc                                          <  2:142608/150‑1 (MQ=255)
                                                                                                             gCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAGTGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAACGCGAAGCCCTCTTTGGCGGCGCGTTGCGCGt                                      >  1:115865/1‑148 (MQ=255)
                                                                                                              ccAGCGGCTGACACATGTCGCTAAGTACCGCATTAGTGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAACGCGAAGCCCTCTTTGGCGGCGCGTTGCGCGTAGat                                  <  2:52011/151‑1 (MQ=255)
                                                                                                               cAGCGGCTGACACATGTCGCTAAGTACCGCATTAGTGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAACGCGAAGCCCTCTTTGGCGGCGCGTTGCGCGTAGat                                  >  1:658489/1‑150 (MQ=255)
                                                                                                                aGCGGCTGACACATGTCGCTAAGTACCGCATTAGTGGCCTGCGCCTGATACGGCGTGGTTTCACACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAACGCGAAGCCGTCTTTGGCGGCGCGTTGCGCGTAGa                                   <  1:62114/148‑1 (MQ=255)
                                                                                                                       gACACATGTCGCTAAGTACCGCATTAGTGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAACGCGAAGCCCTCTTTGGCGGCGCGTTGCGCGTAGATATCCAGCaa                         <  2:75625/151‑1 (MQ=255)
                                                                                                                       gACACATGTCGCTAAGTACCGCATTAGTGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAACGCGAAGCCCTCTTTGGCGGCGCGTTGCGCGTAGATATCCAGCa                          <  2:88147/150‑1 (MQ=255)
                                                                                                                                              ttAGTGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAACGCGAAGCCCTCTTTGGCGGCGCGTTGCGCGTAGATATCCAGCAATTCCGCCGCCACATCACGCACtt  >  2:585850/1‑151 (MQ=255)
                                                                                                                                              ttAGTGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAACGCGAAGCCCTCTTTGGCGGCGCGTTGCGCGTAGATATCCAGCAATTCCGCCGCCACATCACGCACtt  >  2:391867/1‑151 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
AGGCACCAGCACCGCCACCTGCTTGTGGTTATCTACTGCCAGGAAAGCTGCGCGCATCGCCACTTCTGTTTTACCAAAGCCAACATCGCCGCACACCAGACGATCCATTGCCAGCGGCTGACACATGTCGCTAAGTACCGCATTAATGGCCTGCGCCTGATCCGGCGTGGTTTCAAACGGGAAGCTGTCGCAGAACAACTGATACTGCTCACGATCGTGTTTAAACGCGAAGCCCTCTTTGGCGGCGCGTTGCGCGTAGATATCCAGCAATTCCGCCGCCACATCACGCACTT  >  NC_000913/1171994‑1172286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A