Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,687,347	G→A	A21V (GCC→GTC)	glnB ←	regulatory protein P‑II for glutamine synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,687,347	0	G	A	100.0%	187.7 / NA	50	A21V (GCC→GTC)	glnB	regulatory protein P‑II for glutamine synthetase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (17/33); total (17/33)

CAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGGCCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGTAACACATAACGCCAATTCATTCCTTGAAATCGT  >  NC_000913/2687201‑2687485

cAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGAcc                                                                                                                                          >  2:626714/1‑149 (MQ=255)

         ccGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTcgc                                                                                                                                <  1:481577/150‑1 (MQ=255)

              aCGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACa                                                                                                                           <  2:195587/150‑1 (MQ=255)

              aCGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACAt                                                                                                                          <  1:169066/151‑1 (MQ=255)

               cGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATc                                                                                                                         <  1:550153/151‑1 (MQ=255)

                   aaTCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTcc                                                                                                                     <  1:172019/151‑1 (MQ=255)

                            tttCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGaa                                                                                                             <  1:208614/150‑1 (MQ=255)

                             ttCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAg                                                                                                            <  2:539370/150‑1 (MQ=255)

                                 cTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGttt                                                                                                       >  1:418148/1‑151 (MQ=255)

                                   ttCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGtttt                                                                                                      <  1:612526/150‑1 (MQ=255)

                                    tCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTta                                                                                                     <  1:216594/150‑1 (MQ=255)

                                                ccaccaTATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAAt                                                                                        <  2:644253/151‑1 (MQ=255)

                                                    caTATACTCGGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATTATCGCATCACTCtt                                                                                     <  2:633719/150‑1 (MQ=255)

                                                     atatACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCtttt                                                                                   <  2:373345/151‑1 (MQ=255)

                                                       ataCTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCttttt                                                                                  <  2:618487/150‑1 (MQ=255)

                                                         aCTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCAt                                                                               >  2:415761/1‑151 (MQ=255)

                                                            ccgcgccgcgGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCt                                                                            <  2:441500/151‑1 (MQ=255)

                                                             cgcgccgcgGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCt                                                                            >  1:421771/1‑150 (MQ=255)

                                                             cgcgccgcgGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTa                                                                           <  2:571823/151‑1 (MQ=255)

                                                              gcgccgcgGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTAt                                                                          <  2:223781/151‑1 (MQ=255)

                                                              gcgccgcgGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTAt                                                                          >  2:206425/1‑151 (MQ=255)

                                                              gcgccgcgGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTAt                                                                          >  1:105413/1‑151 (MQ=255)

                                                                     ggTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGa                                                                   <  1:419529/151‑1 (MQ=255)

                                                                          aGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAgg                                                              >  1:118043/1‑151 (MQ=255)

                                                                           gCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGt                                                             <  2:462770/151‑1 (MQ=255)

                                                                               ggTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGcc                                                         <  2:476981/151‑1 (MQ=255)

                                                                               ggTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGcc                                                         <  1:592479/151‑1 (MQ=255)

                                                                                gTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCt                                                        >  2:472268/1‑151 (MQ=255)

                                                                                     gCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCtt                                                   <  1:168555/151‑1 (MQ=255)

                                                                                        tttCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTtat                                                                                                    <  2:183339/99‑1 (MQ=255)

                                                                                        tttCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTtat                                                                                                    >  1:183344/1‑99 (MQ=255)

                                                                                         ttCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCtttt                                                 <  2:179377/149‑1 (MQ=255)

                                                                                          tCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGAt                                              >  2:442009/1‑151 (MQ=255)

                                                                                           cTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGATTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTg                                                >  2:377325/1‑148 (MQ=255)

                                                                                             ggCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAATGTCGCCTGTCTTTTGAt                                              <  1:9968/148‑1 (MQ=255)

                                                                                               cGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCt                                         <  1:70185/151‑1 (MQ=255)

                                                                                                           ttCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGTAacac                              <  1:73521/150‑1 (MQ=255)

                                                                                                            tCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGTAACACAt                            >  1:175486/1‑151 (MQ=255)

                                                                                                                 tCGGTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGTAACACATAACGc                       >  2:509046/1‑151 (MQ=255)

                                                                                                                    gTCACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGTAACACATAACGCCaa                    <  2:366005/151‑1 (MQ=255)

                                                                                                                      cACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGTAACACATAACGCCAatt                  >  1:140729/1‑151 (MQ=255)

                                                                                                                      cACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGTAACACATAACGCCAatt                  <  2:101035/151‑1 (MQ=255)

                                                                                                                      cACCGTCATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGTAACACATAACGCCAatt                  <  2:17181/151‑1 (MQ=255)

                                                                                                                            cATGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGTAACACATAACGCCAATTCATTcc            >  2:135819/1‑151 (MQ=255)

                                                                                                                             aTGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGTAACACATAACGCCAATTCATTCCt           <  2:293877/151‑1 (MQ=255)

                                                                                                                             aTGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGTAACACATAACGCCAATTCATTCCt           >  1:293884/1‑151 (MQ=255)

                                                                                                                             aTGCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGTAACACATAACGCCAATTCATTCCt           >  2:162035/1‑151 (MQ=255)

                                                                                                                               gCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGTAACACATAACGCCAATTCATTCCtt          <  2:340107/150‑1 (MQ=255)

                                                                                                                               gCCGGTAATACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGTAACACATAACGCCAATTCATTCCTTg         <  1:22268/151‑1 (MQ=255)

                                                                                                                                      aTACCGACTTCGACCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGTAACACATAACGCCAATTCATTCCTTGAAATCGt  <  2:101069/151‑1 (MQ=255)

CAATGTCGTCCGGTACGACAATCTCAATTTTCACTTTCGGCAGAAAATCCACCATATACTCCGCGCCGCGGTACAGCTCGGTATGGCCTTTCTGGCGACCAAAGCCTTTCACTTCGGTCACCGTCATGCCGGTAATACCGACTTCGGCCAGTGCTTCGCGGACATCGTCCAGCTTGAAGGGTTTTATAATCGCATCAATCTTTTTCATGCTATTCCTTGAAAAGGTCGCCTGTCTTTTGATCTGCTAAACGTAACACATAACGCCAATTCATTCCTTGAAATCGT  >  NC_000913/2687201‑2687485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A