Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,913,196	T→C	K152K (AAA→AAG)	relA ←	(p)ppGpp synthetase I/GTP pyrophosphokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,913,196	0	T	C	100.0%	167.5 / NA	46	K152K (AAA→AAG)	relA	(p)ppGpp synthetase I/GTP pyrophosphokinase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (24/22); total (24/22)

GTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGTTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATCACGCACGCCGTGAATAAGGTTAACG  >  NC_000913/2913049‑2913343

gTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTtg                                                                                                                                                  <  1:295955/151‑1 (MQ=255)

   aTCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTtgatg                                                                                                                                               >  1:202836/1‑151 (MQ=255)

         cAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACg                                                                                                                                         >  2:79666/1‑151 (MQ=255)

                      aGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAatcat                                                                                                                            >  2:310006/1‑151 (MQ=255)

                      aGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAatcat                                                                                                                            <  1:547761/151‑1 (MQ=255)

                       gTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAatcatc                                                                                                                           <  1:560297/151‑1 (MQ=255)

                         tGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCg                                                                                                                          <  1:330535/150‑1 (MQ=255)

                         tGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCg                                                                                                                          <  1:38586/150‑1 (MQ=255)

                              gATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATc                                                                                                                    >  1:371453/1‑151 (MQ=255)

                                  ccGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGcc                                                                                                                 >  2:459085/1‑150 (MQ=255)

                                          gTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATc                                                                                                        <  1:116159/151‑1 (MQ=255)

                                          gTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATc                                                                                                        <  2:184748/151‑1 (MQ=255)

                                           ttAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATcc                                                                                                       >  2:635809/1‑151 (MQ=255)

                                            tAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATcc                                                                                                       <  1:110169/150‑1 (MQ=255)

                                            tAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATcc                                                                                                       <  2:467744/150‑1 (MQ=255)

                                                 aGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGa                                                                                                  >  1:501055/1‑150 (MQ=255)

                                                   cGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACg                                                                                               >  2:198055/1‑151 (MQ=255)

                                                       gCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTa                                                                                            <  1:523337/150‑1 (MQ=255)

                                                       gCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTAt                                                                                           <  1:600098/151‑1 (MQ=255)

                                                            gATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGAcc                                                                                      >  2:122980/1‑151 (MQ=255)

                                                                ttGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGtt                                                                                  >  2:655230/1‑151 (MQ=255)

                                                                ttGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGtt                                                                                  >  1:7615/1‑151 (MQ=255)

                                                                  ggTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTc                                                                                 <  2:376958/150‑1 (MQ=255)

                                                                   gTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCgg                                                                               <  2:524849/151‑1 (MQ=255)

                                                                     acacTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCgga                                                                              <  1:421749/150‑1 (MQ=255)

                                                                                          acGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTc                                                        <  1:529388/151‑1 (MQ=255)

                                                                                                     ccGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTg                                             >  2:395952/1‑151 (MQ=255)

                                                                                                     ccGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTg                                             >  2:447639/1‑151 (MQ=255)

                                                                                                       ggCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGc                                           <  2:341623/151‑1 (MQ=255)

                                                                                                         cgcATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCgg                                         >  1:154248/1‑151 (MQ=255)

                                                                                                           cATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGAt                                       >  1:126937/1‑151 (MQ=255)

                                                                                                             tCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATcg                                     >  2:167501/1‑151 (MQ=255)

                                                                                                               ttttACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATcgcc                                   >  2:677839/1‑151 (MQ=255)

                                                                                                               ttttACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATcgcc                                   >  1:589058/1‑151 (MQ=255)

                                                                                                                tttACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATcgccg                                  >  1:457604/1‑151 (MQ=255)

                                                                                                                tttACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATcg                                     <  2:114231/148‑1 (MQ=255)

                                                                                                                tttACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATc                                      <  2:165660/147‑1 (MQ=255)

                                                                                                                tttACTTCGCGCAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATc                                      <  2:233182/147‑1 (MQ=255)

                                                                                                                          gcAGATGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCGGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATcacg                        <  1:169284/151‑1 (MQ=255)

                                                                                                                              aTGAGCAATACGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATcacgcacg                    >  1:39834/1‑151 (MQ=255)

                                                                                                                                        cGCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATCACGCACGCCGTGAATa           >  1:94370/1‑150 (MQ=255)

                                                                                                                                         gCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATCACGCACGCCGTGAATa           >  1:120321/1‑149 (MQ=255)

                                                                                                                                         gCTCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATCACGCACGCCGTGAATa           >  2:124076/1‑149 (MQ=255)

                                                                                                                                           tCCGCCAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATCACGCAAGCCGTGAATAAGGt       >  1:354285/1‑151 (MQ=255)

                                                                                                                                               ccAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATCACGCACGCCGTGAATAAGGTTAAc   <  2:169867/151‑1 (MQ=255)

                                                                                                                                                cAGCTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATACCACCCGGCGAACGTTATCGCCCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATCACGCACGCCGTGAATAAGGTTAAcg  <  2:328124/151‑1 (MQ=255)

GTAATCTTCCAGTTCCCATTTCAGTTGTCCGATTCCGAGACGGTTAGCCAGCGGTGCGTAGATGTTGGTACACTCTTTTGCCGCCAGTACACGTTCATCTTCCGGCGCATCTTTTACTTCGCGCAGATGAGCAATACGCTCCGCCAGTTTGATGACTACGCAGCGAAAATCATCGACCATCGCCAATAACATCCGGCGAACGTTATCGACCTGTTCGGAGGAAACAGAATCAGTGTGCGTCGCTTTCAGCTGGCGGATCGCCGCCATATCACGCACGCCGTGAATAAGGTTAACG  >  NC_000913/2913049‑2913343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A