Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,968,723	A→G	R85R (CGT→CGC)	rppH ←	RNA pyrophosphohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,968,723	0	A	G	100.0%	174.0 / NA	47	R85R (CGT→CGC)	rppH	RNA pyrophosphohydrolase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (22/25); total (22/25)

TCTGACCGGATACCAGTAACTTACCCATCGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGTACATCGCCTGCTCTGCGGATTCTCCGGGGTTGATTCCG  >  NC_000913/2968576‑2968864

tCTGACCGGATACCAGTAACTTACCCATCGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAgcgc                                                                                                                                            >  1:580705/1‑151 (MQ=255)

   gACCGGATACCAGTAACTTACCCATCGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCAcc                                                                                                                                         <  1:533445/151‑1 (MQ=255)

      cGGATACCAGTAACTTACCCATCGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCaa                                                                                                                                       >  2:47563/1‑150 (MQ=255)

             cAGTAACTTACCCATCGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCg                                                                                                                               <  2:292380/151‑1 (MQ=255)

               gTAACTTACCCATCGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGt                                                                                                                             <  2:62036/151‑1 (MQ=255)

                 aaCTTACCCATCGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTaa                                                                                                                           <  1:394740/151‑1 (MQ=255)

                  aCTTACCCATCGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAAt                                                                                                                          <  2:19125/151‑1 (MQ=255)

                  aCTTACCCATCGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAAt                                                                                                                          <  1:58980/151‑1 (MQ=255)

                      aCCCATCGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGt                                                                                                                      >  2:441550/1‑151 (MQ=255)

                         cATCGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAgc                                                                                                                   >  1:255477/1‑151 (MQ=255)

                          aTCGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAgcg                                                                                                                  >  1:10851/1‑151 (MQ=255)

                            cGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAgcgc                                                                                                                 >  2:563317/1‑150 (MQ=255)

                                aGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAAcc                                                                                                             >  2:101189/1‑150 (MQ=255)

                                aGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCa                                                                                                            <  2:190467/151‑1 (MQ=255)

                                  ccGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGt                                                                                                          >  1:588665/1‑151 (MQ=255)

                                    gTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTa                                                                                                        <  1:360928/151‑1 (MQ=255)

                                      cAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAATCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTAc                                                                                                       <  1:519406/150‑1 (MQ=255)

                                       aaaCTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTAcgc                                                                                                     >  2:134040/1‑151 (MQ=255)

                                        aaCTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTAcgcg                                                                                                    <  1:608616/151‑1 (MQ=255)

                                            ctGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGtt                                                                                                  <  2:126205/149‑1 (MQ=255)

                                                 gtACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCaa                                                                                           <  2:587555/151‑1 (MQ=255)

                                                    ctgctgGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAgg                                                                                         <  2:44863/150‑1 (MQ=255)

                                                           tttGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAAc                                                                                 >  2:321130/1‑151 (MQ=255)

                                                                     gATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCgg                                                                       >  1:175218/1‑151 (MQ=255)

                                                                       tttCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCt                                                                     >  1:299089/1‑151 (MQ=255)

                                                                         tCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCtt                                                                    <  2:134951/150‑1 (MQ=255)

                                                                                  ccgcTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTActt                                                          <  2:627379/151‑1 (MQ=255)

                                                                                      tCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACttcttc                                                      >  1:162331/1‑151 (MQ=255)

                                                                                       cACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACttcttc                                                      <  1:322528/150‑1 (MQ=255)

                                                                                         ccAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCaaa                                                   <  1:405415/151‑1 (MQ=255)

                                                                                          cAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAAc                                                  <  2:532916/151‑1 (MQ=255)

                                                                                              tGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAAtt                                              >  1:497124/1‑151 (MQ=255)

                                                                                                 aaGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCAc                                           >  2:280917/1‑151 (MQ=255)

                                                                                                     gaAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGTa                                       <  1:358239/151‑1 (MQ=255)

                                                                                                        aCCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGTACAt                                    >  2:539777/1‑151 (MQ=255)

                                                                                                                   ttttgGCCGCTACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGGCTTTGCGGCTTAATCCTAATTCTTCAAACAATTCACGGTACATCGCCTGctc                           >  2:514275/1‑149 (MQ=255)

                                                                                                                   ttttgGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGTACATCGCCTGCTCTg                         >  2:309080/1‑151 (MQ=255)

                                                                                                                   ttttgGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGTACATCGCCTGCTCTg                         >  1:280006/1‑151 (MQ=255)

                                                                                                                     ttgGCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGTACATCGCCTGCTCTGc                        <  2:190817/150‑1 (MQ=255)

                                                                                                                       ggCCGATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGTACATCGCCTGCTCTGCGGa                     <  2:76636/151‑1 (MQ=255)

                                                                                                                           gATACAAACCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGTACATCGCCTGCTCTGCGGATtct                 >  1:673058/1‑151 (MQ=255)

                                                                                                                                aaaCCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGTACATCGCCTGCTCTGCGGATTCTCCggg            >  2:422583/1‑151 (MQ=255)

                                                                                                                                 aaCCGGCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGTACATCGCCTGCTCTGCGGATTCTCCgggg           <  1:56204/151‑1 (MQ=255)

                                                                                                                                      gCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGTACATCGCCTGCTCTGCGGATTCTCCGGGGTTGAt      <  2:334746/151‑1 (MQ=255)

                                                                                                                                      gCTTCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGTACATCGCCTGCTCTGCGGATTCTCCGGGGTTGAt      >  2:559358/1‑151 (MQ=255)

                                                                                                                                        ttCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGTACATCGCCTGCTCTGCGGATTCTCCGGGGTTGATTc    >  2:14616/1‑151 (MQ=255)

                                                                                                                                         tCGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGTACATCGCCTGCTCTGCGGATTCTCCGGGGTTGATTc    <  1:412098/150‑1 (MQ=255)

                                                                                                                                          cGTGTCCCAGCGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGTACATCGCCTGCTCTGCGGATTCTCCGGGGTTGATTCCg  <  1:557708/151‑1 (MQ=255)

TCTGACCGGATACCAGTAACTTACCCATCGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGTACATCGCCTGCTCTGCGGATTCTCCGGGGTTGATTCCG  >  NC_000913/2968576‑2968864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A