Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,037,738	A→T	D123E (GAT→GAA)	recJ ←	ssDNA exonuclease, 5' ‑‑> 3'‑specific

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,037,738	0	A	T	100.0%	167.3 / NA	42	D123E (GAT→GAA)	recJ	ssDNA exonuclease, 5' ‑‑> 3'‑specific
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (21/21); total (21/21)

CCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCG  >  NC_000913/3037593‑3037876

ccGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGTTCGAc                                                                                                                                       <  2:355092/151‑1 (MQ=255)

  gCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGTTCGAcc                                                                                                                                      >  2:299708/1‑150 (MQ=255)

  gCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGTTCGACCa                                                                                                                                     <  1:124347/151‑1 (MQ=255)

          tAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGTTCGACCACTTCCggg                                                                                                                             >  1:679135/1‑151 (MQ=255)

                         cAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGTTCGACCACTTCCGGGCTTAAGCCGTAACCg                                                                                                              >  2:557196/1‑151 (MQ=255)

                           aaTGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGTTCGACCACTTCCGGGCTTAAGCCGTAACCGTc                                                                                                            >  2:36374/1‑151 (MQ=255)

                              ggTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGTTCGACCACTTCCGGGCTTAAGCCGTAACCGTCtt                                                                                                          <  1:272034/150‑1 (MQ=255)

                                 gATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGTTCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGa                                                                                                       <  2:79586/150‑1 (MQ=255)

                                  aTCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGTTCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGaa                                                                                                      >  2:157940/1‑150 (MQ=255)

                                     ggTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGTTCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACgg                                                                                                  >  1:512208/1‑151 (MQ=255)

                                         aCAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGTTCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTg                                                                                              <  2:424742/151‑1 (MQ=255)

                                          cAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGTTCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTgg                                                                                             >  2:330224/1‑151 (MQ=255)

                                            aTAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGTTCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTa                                                                                           <  1:523022/151‑1 (MQ=255)

                                            aTAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGTTCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTa                                                                                           <  1:144760/151‑1 (MQ=255)

                                              aaCCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGTTCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTAcc                                                                                         >  1:465949/1‑151 (MQ=255)

                                                  gggATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGTTCGACCACTTCCGGGCTTAAGCCGTAACCGACTTCGAAACGGTTTGGTACCAGGt                                                                                     >  2:449500/1‑151 (MQ=255)

                                                     aTGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGTTCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGt                                                                                  <  1:68220/151‑1 (MQ=255)

                                                     aTGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGTTCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGt                                                                                  <  1:542129/151‑1 (MQ=255)

                                                       gCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGTTCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCg                                                                                >  2:666320/1‑151 (MQ=255)

                                                                gcgagcgTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGTTCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATtgctg                                                                       >  1:480331/1‑151 (MQ=255)

                                                                  gagcgTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGTTCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATtgctgc                                                                      >  1:105192/1‑150 (MQ=255)

                                                                                  cGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGTTCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATc                                                     <  2:117095/151‑1 (MQ=255)

                                                                                        ggaggaAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGTTCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGc                                               <  1:343569/151‑1 (MQ=255)

                                                                                             aaaTACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGTTCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCt                                          >  2:417115/1‑151 (MQ=255)

                                                                                                   cGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGTTCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGc                                    <  2:347718/151‑1 (MQ=255)

                                                                                                       aTCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGTTCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTg                                <  1:185976/151‑1 (MQ=255)

                                                                                                             cGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGTTCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCGCGCTTAGAGCCGTGCTGGTc                          <  2:404636/151‑1 (MQ=255)

                                                                                                              gTGACAATTAACTGCGCGCCACGGGCATGGGCCTGTCCGACCACTTCCGCGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCCGGTAGGCGATATTGCTGCAACCAACCGACCGCATCGCCACCACCCTCAGAGCCGTGCTGGTc                          >  2:518087/1‑150 (MQ=255)

                                                                                                                gACAATTAACTGCGCGCCACGGGCATGGGCCTGTTCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTcgc                        <  1:266369/150‑1 (MQ=255)

                                                                                                                 aCAATTAACTGCGCGCCACGGGCATGGGCCTGTTCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCgcgc                      >  2:272391/1‑151 (MQ=255)

                                                                                                                         cTGCGCGCCACGGGCATGGGCCTGTTCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCg              >  2:340988/1‑151 (MQ=255)

                                                                                                                           gcgcgcCACGGGCATGGGCCTGTTCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTc            >  2:459552/1‑151 (MQ=255)

                                                                                                                           gcgcgcCACGGGCATGGGCCTGTTCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTc            <  1:124249/151‑1 (MQ=255)

                                                                                                                            cgcgcCACGGGCATGGGCCTGTTCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCg           <  1:332858/151‑1 (MQ=255)

                                                                                                                             gcgcCACGGGCATGGGCCTGTTCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGa          <  2:200844/151‑1 (MQ=255)

                                                                                                                               gcCACGGGCATGGGCCTGTTCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGaaa        <  2:244333/151‑1 (MQ=255)

                                                                                                                               gcCACGGGCATGGGCCTGTTCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGaa         >  1:624952/1‑150 (MQ=255)

                                                                                                                                ccACGGGCATGGGCCTGTTCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAAt       >  1:144297/1‑151 (MQ=255)

                                                                                                                                 cACGGGCATGGGCCTGTTCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGCCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATc      >  1:395137/1‑151 (MQ=255)

                                                                                                                                  aCGGGCATGGGCCTGTTCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCa     <  2:406967/151‑1 (MQ=255)

                                                                                                                                    gggCATGGGCCTGTTCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCAcc   <  2:168411/151‑1 (MQ=255)

                                                                                                                                      gCATGGGCCTGTTCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCg  >  2:287683/1‑150 (MQ=255)

CCGCTGCGGGTAATGTGTCGCCTGGCAAATGGTGATCGGTAACAATAACCGGGATGCCCAACGAGCGAGCGTGCTCAACCCCCGCATGGGAGGAAATACCGTTATCCACCGTGACAATTAACTGCGCGCCACGGGCATGGGCCTGATCGACCACTTCCGGGCTTAAGCCGTAACCGTCTTCGAAACGGTTTGGTACCAGGTAGTCGATATTGCTGCAACCAAGCGAGCGCATCGCCAGCACGCTTAGAGCCGTGCTGGTCGCGCCGTCGGCGTCGAAATCACCG  >  NC_000913/3037593‑3037876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A