Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,068,614	C→T	S65S (TCG→TCA)	argO ←	arginine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,068,614	0	C	T	97.7%	150.2 / NA	43	S65S (TCG→TCA)	argO	arginine transporter
Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (28/14); total (29/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATAATTTTCCATCTGCCTTGCTTCATGACTTCGGCGCTGGCTAACTCAATATTACTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGCGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAG  >  NC_000913/3068473‑3068760

gATAATTTTCCATCTGCCTTGCTTCATGACTTCGGCGCTGGCTAACTCAATATTACTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATc                                                                                                                                           >  1:455571/1‑151 (MQ=255)

gATAATTTTCCATCTGCCTTGCTTCATGACTTCGGCGCTGGCTAACTCAATATTACTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATc                                                                                                                                           <  2:447245/151‑1 (MQ=255)

 aTAATTTTCCATCTGCCTTGCTTCATGACTTCGGCGCTGGCTAACTCAATATTACTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATc                                                                                                                                           >  1:453068/1‑150 (MQ=255)

       ttCCATCTGCCTTGCTTCATGACTTCGGCGCTGGCTAACTCAATATTACTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAAcg                                                                                                                                    >  1:301720/1‑151 (MQ=255)

       ttCCATCTGCCTTGCTTCATGACTTCGGCGCTGGCTAACTCAATATTACTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAAc                                                                                                                                     <  1:504983/150‑1 (MQ=255)

        tCCATCTGCCTTGCTTCATGACTTCGGCGCTGGCTAACTCAATATTACTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGTCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAAcgc                                                                                                                                   >  1:9288/1‑151 (MQ=255)

         ccATCTGCCTTGCTTCATGACTTCGGCGCTGGCTAACTCAATATTACTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAAcgcg                                                                                                                                  <  2:467105/151‑1 (MQ=255)

                     cTTCATGACTTCGGCGCTGGCTAACTCAATATTACTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCaaa                                                                                                                       >  1:336526/1‑150 (MQ=255)

                       tCATGACTTCGGCGCTGGCTAACTCAATATTACTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCaaaa                                                                                                                      <  1:530101/149‑1 (MQ=255)

                        cATGACTTCGGCGCTGGCTAACTCAATATTACTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATc                                                                                                                   >  1:278329/1‑151 (MQ=255)

                         aTGACTTCGGCGCTGGCTAACTCAATATTACTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATc                                                                                                                   <  1:627834/150‑1 (MQ=255)

                           gACTTCGGCGCTGGCTAACTCAATATTACTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATccc                                                                                                                 <  2:595770/150‑1 (MQ=255)

                            aCTTCGGCGCTGGCTAACTCAATATTACTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCgg                                                                                                               >  1:448224/1‑151 (MQ=255)

                                                aaTATTACTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCg                                                                                           >  1:310486/1‑151 (MQ=255)

                                                aaTATTACTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCg                                                                                           >  1:226482/1‑151 (MQ=255)

                                                   atTACTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCt                                                                                         <  2:675295/150‑1 (MQ=255)

                                                    ttACTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCCAATCAGGACCAAATCGCTg                                                                                        >  2:629090/1‑150 (MQ=255)

                                                    ttACTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTg                                                                                        >  2:251493/1‑150 (MQ=255)

                                                     tACTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGAt                                                                                      >  2:428485/1‑151 (MQ=255)

                                                     tACTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGAt                                                                                      >  2:449682/1‑151 (MQ=255)

                                                      aCTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGAt                                                                                      >  2:160750/1‑150 (MQ=255)

                                                               tGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGt                                                                            >  2:287151/1‑151 (MQ=255)

                                                                    ttttAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGc                                                                       >  2:84992/1‑151 (MQ=255)

                                                                      ttAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCa                                                                      >  1:613168/1‑150 (MQ=255)

                                                                      ttAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCa                                                                      >  2:644237/1‑150 (MQ=255)

                                                                      ttAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCa                                                                      >  2:542705/1‑150 (MQ=255)

                                                                          aaGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCAt                                                                 <  1:400268/151‑1 (MQ=255)

                                                                             cgcCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAAt                                                              <  1:549997/151‑1 (MQ=255)

                                                                               ccAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAAtgt                                                            >  1:627912/1‑151 (MQ=255)

                                                                               ccAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAAtgt                                                            <  2:336516/151‑1 (MQ=255)

                                                                                 aaaaCCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTgg                                                          >  1:378226/1‑151 (MQ=255)

                                                                                    aCCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGCGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTa                                                        >  2:108280/1‑150 (MQ=255)

                                                                                       aTACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTg                                                     >  2:550246/1‑150 (MQ=255)

                                                                                         aCCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTgac                                                   <  2:162114/150‑1 (MQ=255)

                                                                                                cAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATg                                           <  1:401270/151‑1 (MQ=255)

                                                                                                    aagGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCt                                       >  2:214450/1‑151 (MQ=255)

                                                                                                         tACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATtc                                  >  2:499607/1‑151 (MQ=255)

                                                                                                                 cccAGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACaaa                          <  2:215649/151‑1 (MQ=255)

                                                                                                                    aGGTGACCAGCGCCAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGc                       >  1:377101/1‑151 (MQ=255)

                                                                                                                                 cAGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCg          >  2:450256/1‑151 (MQ=255)

                                                                                                                                  aGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCg          >  1:562888/1‑150 (MQ=255)

                                                                                                                                  aGCAACCACGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCACGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCg          >  2:102397/1‑150 (MQ=255)

                                                                                                                                         aCGGTGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAg  <  2:99284/151‑1 (MQ=255)

GATAATTTTCCATCTGCCTTGCTTCATGACTTCGGCGCTGGCTAACTCAATATTACTGCTCATTGCTGTTTTAAAAGCGCCAAAACCATACCACAGCAAGAAGGCTACGCCGCCCCAGGTGACCAGCGCCAGCAACCACGGCGACTGCATCAATAACGCGCTGCCACCAAAAATCCCGGCGCAAATCAGGACCAAATCGCTGATAGCACAAAGTAAGGCAATCATAATGTGGTACTGACGACGTATGCCCTGATTCATCACAAAAGCATTTTGTGGACCGAGCGGTAG  >  NC_000913/3068473‑3068760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A