Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F50 I2 R1 209 44.2 1360995 98.7% 1343302 150.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,163,010 G→A P158S (CCG→TCG)  plsC ← 1‑acyl‑sn‑glycerol‑3‑phosphate acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,163,0100GA100.0% 187.2 / NA 48P158S (CCG→TCG) plsC1‑acyl‑sn‑glycerol‑3‑phosphate acyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/23);  total (25/23)

GACGTCAATTGGCGGCAGCATTTCGACAATCACCAGACCGTTGTGCAGTCGATTAAGATTAATCTTATTCGAAGTTGTAGAGACGCACACGGGAATAATCGGGACGC‑CCGCCGCAATTGCCGCGTGAAATGCTCCAGTCTTGAACGGTAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTG  >  NC_000913/3162864‑3163146
                                                                                                                                                   |                                                                                                                                        
gacGTCAATTGGCGGCAGCATTTCGACAATCACCAGACCGTTGTGCAGTCGATTAAGATTAATCTTATTCGAAGTTGTAGAGACGCACACGGGAATAATCGGGACGC‑CCGCCGCAATTGCCGCGTGAAATGCTCCAGTCTTGAACGATa                                                                                                                                        <  1:304242/149‑1 (MQ=255)
      aaTTGGCGGCAGCATTTCGACAATCACCAGACCGTTGTGCAGTCGATTAAGATTAATCTTATTCGAAGTTGTAGAGACGCACACGGGAATAATCGGGACGC‑CCGCCGCAATTGCCGCGTGAAATGCTCCAGTCTTGAACGATAGCAGGCcg                                                                                                                                >  2:30210/1‑151 (MQ=255)
         tGGGGGCAGCATTCCGTCAATCACCAGACCGTTGTGCAGTCGATTAAGATTAATCTTATTCGAAGTTGTAGGGACGCACACGGGAATAATCGGGACGC‑CCGCCGCAATTGCCGCGTGAAATGCTCCAGTCTTGAACGATAGCAGGCcgcg                                                                                                                              <  1:26007/150‑1 (MQ=255)
          ggcggcAGCATTTCGACAATCCCCAGACCGTTTTGCATGCGATTAAGATTAATCTTATCCGAAGTTGTAGAGACGCACACGGGAATAAGCGGGACGC‑CCGCCGCAATTGCCGCGTGAAATGCTCCAGTCTTGAACGATAGCAGGCcgcg                                                                                                                              <  2:472741/149‑1 (MQ=255)
                     ttCGACAATCACCAGACCGTTGTGCAGTCGATTAAGATTAATCTTATTCGAAGTTGTAGAGACGCACACGGGAATAATCGGGACGC‑CCGCCGCAATTGCCGCGTGAAATGCTCCAGTCTTGAACGATAGCAGGCCGCGACCACGGCTGCg                                                                                                                  <  2:58285/150‑1 (MQ=255)
                      tCGACAATCACCAGACCGTTGTGCAGTCGATTAAGATTAATCTTATTCGAAGTTGTAGAGACGCACACGGGAATAATCGGGACGC‑CCGCCGCAATTGCCGCGTGAAATGCTCCAGTCTTGAACGATAGCAGGCCGCGACCACGGCTGCggg                                                                                                                >  2:263311/1‑151 (MQ=255)
                      tCGACAATCACCAGACCGTTGTGCAGTCGATTAAGATTAATCTTATTCGAAGATGTAGAGACGCACACGGGAATAATCGGGACGC‑ACGCCGCAATTGCCGCGTGAAATGCTCCAGTCTTGAACGATAGCAGGCCGCGACCACGGCTGCggg                                                                                                                >  2:423071/1‑151 (MQ=255)
                                         tgtgCAGTCGATTAAGATTAATCTTATTCGAAGTTGTAGAGACGCACACGGGAATAATCGGGACGC‑CCGCCGCAATTGCCGCGTGAAATGCTCCAGTCTTGAACGATAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATcc                                                                                              >  1:673483/1‑150 (MQ=255)
                                          gtgCAGTCGATTAAGATTAATCTTATTCGAAGTTGTAGAGACGCACACGGGAATAATCGGGACGC‑CCGCCGCAATTGCCGCGTGAAATGCTCCAGTCTTGAACGATAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCa                                                                                             <  2:658273/150‑1 (MQ=255)
                                                tCGATTAAGATTAATCTTATTCGAAGTTGTAGAGACGCACACGGGAATAATCGGGACGC‑CCGCCGCAATTGCCGCGTGAAATGCTCCAGTCTTGAACGATAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGa                                                                                       >  2:563251/1‑150 (MQ=255)
                                                  gataaagattagTTTAGTACGAGGGTGTAGCGACCCACTGGGGAATAA‑AGGCATGCTCCGCCGCAATTGCCGCGTGAAATGCTCCAGTCTTGAACGATAGCAGGCCGCGACCGCGGCTGCGGGTTCCTTCCGGGAACATCCAg                                                                                            <  2:347391/143‑1 (MQ=255)
                                                   attaagattatTCTTATTCGAATTTGTAGAGACGCACACGGGAATAATCGGGACGC‑CCGCCGCAATTGCCGCGTGAAATTCTCCAGTCTTGAACGATAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATa                                                                                   <  2:512799/151‑1 (MQ=255)
                                                   attaagattaaTCTTATTCGAAGTTGTAGAGACGCACACGGGAATAATCGGGACGC‑CCGCCGCAATTGCCGCGTGAAATGCTCCAGTCTTGAACGATAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAAt                                                                                    <  1:585234/150‑1 (MQ=255)
                                                    ttaagattaaTCTTATTCGAAGTTGTAGAGACGCACACGGGAATAATCGGGACGC‑CCGCCGCAATTGCCGCGTGAAATGCTCCAGTCTTGAACGATAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATAc                                                                                  >  2:560380/1‑151 (MQ=255)
                                                     taagattaaTCTTATTCGAAGTTGTAGAGACGCACACGGAAATAATCGGGACGC‑CCGCCGCAATTGCCGCGTGAAATGCTCCAGTCTTGAACGATAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATAc                                                                                  <  1:213256/150‑1 (MQ=255)
                                                       agattaaTCTTATTCGAAGTTGTAGAGACGCACACGGGAATAATCGGGACGC‑CCGCCGCAATTGCCGCGTGAAATGCTCCAGTCTTGAACGATAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGc                                                                               <  1:372516/151‑1 (MQ=255)
                                                            aaTCTTATTCGAAGTTGTAGAGCCGCACCGGGGAATAATCGGGACGC‑CCGCCGCAATTGCCGCGTGAAATGCTCCAGTCTTGAACGATAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGtttt                                                                          <  2:534863/151‑1 (MQ=255)
                                                                 tATTCGAAGTTGTAGAGACGCACACGGGAATAATCGGGACGC‑CCGCCGCAATTGCCGCGTGAAATGCTCCAGTCTTGAACGATAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGa                                                                      >  2:400976/1‑150 (MQ=255)
                                                                    tCGAAGTTGTAGAGACGCACACGGGAATAATCGGGACGC‑CCGCCGCAATTGCCGCGTGAAATGCTCCAGTCTTGAACGATAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTg                                                                  >  2:257175/1‑151 (MQ=255)
                                                                          ttGTAGAGACGCACACGGGAATAATCGGGACGC‑CCGCCGCAATTGCCGCGTGAAATGCTCCAGTCTTGAACGATAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCac                                                            >  2:280094/1‑151 (MQ=255)
                                                                             tAGAGACGCACACGGGAATAATCGGGACGC‑CCGCCGCAATTGCCGCGTGAAATGCTCCAGTCTTGAACGATAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCact                                                           <  1:193346/149‑1 (MQ=255)
                                                                                 gaCGCACACGGGAATAATCGGGACGC‑CCGCCGCAATTGCCGCGTGAAATGCTCCAGTCTTGAACGATAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTcc                                                     <  1:156867/151‑1 (MQ=255)
                                                                                    gCACACGGGAATAATCGGGACGC‑CCGCCGCAATTGCCGCGTGAAATGCTCCAGTCTTGAACGATAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGc                                                   >  1:343737/1‑150 (MQ=255)
                                                                                     cgcacGGGAATAATCGGGACGC‑CCGCCGCAATTGCCGCGTGAAATGCTCCAGTCTTGAACGATAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGc                                                   >  2:479439/3‑149 (MQ=255)
                                                                                     cacaCGGGAATAATCGGGACGC‑CCGCCGCAATTGCCGCGTGAAATGCTCCAGTCTTGAACGATAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCa                                                  >  1:212125/1‑150 (MQ=255)
                                                                                      acacGGGAATAATCGGGACGC‑CCGCCGCAATTGCCGCGTGAAATGCTCCAGTCTTGAACGATAGCAGGCTGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCa                                                  <  2:530073/149‑1 (MQ=255)
                                                                                      acacGGGAATAATCGGGACGC‑CCGCCGCAATTGCCGCGTGAAATGCTCCAGTCTTGAACGATAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAAt                                                >  1:328476/1‑151 (MQ=255)
                                                                                        acGGGAATAATCGGGACGC‑CCGCCGCAATTGCCGCGTGAAATGCTCCAGTCTTGAACGATAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATgg                                              >  2:225709/1‑151 (MQ=255)
                                                                                          gggAATAATCGGCACGC‑CCGCCGCAATTGCCGCGTGAAATGCTCCAGTCTTGAACGATAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGt                                             <  1:632159/150‑1 (MQ=255)
                                                                                           ggAATAATCGGGACGC‑CCGCCGCAATTGCCGCGTGAAATGCTCCAGTCTTGAACGATAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGc                                           <  1:599321/151‑1 (MQ=255)
                                                                                            gAATAATCGGGACGC‑CCGCCGCAATTGCCGCGTGAAATGCTCCAGTCTTGAACGATAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGcc                                          >  2:186209/1‑151 (MQ=255)
                                                                                               taatCGGGACGC‑CCGCCGCAATTGCCGCGTGAAATGCTCCAGTCTTGAACGATAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTg                                       >  1:546371/1‑151 (MQ=255)
                                                                                                aatCGGGACGC‑CCGCCGCAATTGCCGCGTGAAATGCTCCAGTCTTGAACGATAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTg                                       >  1:201698/1‑150 (MQ=255)
                                                                                                    gggACGC‑CCGCCGCAATTGCCGCGTGAAATGCTCCAGTCTTGAACGATAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCtt                                  <  1:506420/151‑1 (MQ=255)
                                                                                                     ggACGC‑CCGCCGCAATTGCCGCGTGAAATGCTCCAGTCTTGAACGATAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCttt                                 >  2:294879/1‑151 (MQ=255)
                                                                                                      gACGC‑CCGCCGCAATTGCCGCGTGAAATGCTCCAGTCTTGAACGATAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTa                                <  1:227003/151‑1 (MQ=255)
                                                                                                       aCGC‑CCGCCGCAATTGCCGCGTGAAATGCTCCAGTCTTGAACGATAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCttt                                 >  1:196507/1‑149 (MQ=255)
                                                                                                       aCGC‑CCGCCGCAATTGCCGCGTGAAATGCTCCAGTCTTGAACGATAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCttt                                 >  2:142151/1‑149 (MQ=255)
                                                                                                       aCGC‑CCGCCGCAATTGCCGCGTGAAATGCTCCAGTCTTGAACGATAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGAGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCttt                                 >  1:61979/1‑149 (MQ=255)
                                                                                                            ccgccgCAATTGCCGCGTGAAATGCTCCAGTCTTGAACGATAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCg                           <  1:536235/151‑1 (MQ=255)
                                                                                                                 gcAATTGCCGCGTGAAATGCTCCAGTCTTGAACGATAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGt                      <  1:492041/151‑1 (MQ=255)
                                                                                                                   aaTTGCCGCGTGAAATGCTCCAGTCTTGAACGATAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGttt                    <  2:253021/151‑1 (MQ=255)
                                                                                                                        ccGCGTGAAATGCTCCAGTCTTGAACGATAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTc               >  1:498252/1‑151 (MQ=255)
                                                                                                                          gcgTGAAATGCTCCAGTCTTGAACGATAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCg              >  2:466048/1‑150 (MQ=255)
                                                                                                                          gcgTGAAATGCTCCAGTCTTGAACGATAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCg              >  1:442551/1‑150 (MQ=255)
                                                                                                                          gcgTGAAATGCTCCAGTCTTGAACGATAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGa             <  2:297359/151‑1 (MQ=255)
                                                                                                                                     tCCAGTCTTGAACGATAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTg  >  1:12647/1‑151 (MQ=255)
                                                                                                                                      ccAGTCTTGAACGATAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTg  <  2:486093/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                        
GACGTCAATTGGCGGCAGCATTTCGACAATCACCAGACCGTTGTGCAGTCGATTAAGATTAATCTTATTCGAAGTTGTAGAGACGCACACGGGAATAATCGGGACGC‑CCGCCGCAATTGCCGCGTGAAATGCTCCAGTCTTGAACGGTAGCAGGCCGCGACCACGGCTGCGGGTTCCTTCCGGGAACATCCAGATGGAAATACGGCGTTTTTTGAAGTGATTCACTACTTCCGCAATGGTGCCGTGAGCTTTAGTGCGATTGTTTCTGTCGATCAATAAGTTG  >  NC_000913/3162864‑3163146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A