Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,211,471	G→A	G122D (GGT→GAT)	dnaG →	DNA primase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,211,471	0	G	A	100.0%	133.9 / NA	34	G122D (GGT→GAT)	dnaG	DNA primase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (16/18); total (16/18)

CTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCG  >  NC_000913/3211326‑3211618

cTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGATCTg                                                                                                                                                 >  2:207783/1‑150 (MQ=255)

               aaGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGATCTGAATACGTTTTACcaac                                                                                                                                 >  2:561579/1‑151 (MQ=255)

                       gTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGATCTGAATACGTTTTACCAACAATCTTTa                                                                                                                         <  1:103478/151‑1 (MQ=255)

                          cgtcgAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGATCTGAATACGTTTTACCAACAATCTTTaca                                                                                                                       <  1:455600/150‑1 (MQ=255)

                           gtcgAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGATCTGAATACGTTTTACCAACAATCTTTacaac                                                                                                                     <  1:266285/151‑1 (MQ=255)

                                  cGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGATCTGAATACGTTTTACCAACAATCTTTACAACAACCTGt                                                                                                              <  2:493934/151‑1 (MQ=255)

                                       gAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGATCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCa                                                                                                         <  1:300825/151‑1 (MQ=255)

                                        aaGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGATCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCAc                                                                                                        >  1:441831/1‑151 (MQ=255)

                                        aaGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGATCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCAc                                                                                                        <  1:371266/151‑1 (MQ=255)

                                         agagCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGATCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACg                                                                                                       >  1:80715/1‑151 (MQ=255)

                                           agCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGATCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTc                                                                                                     <  1:647787/151‑1 (MQ=255)

                                           agCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGATCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTc                                                                                                     >  1:347457/1‑151 (MQ=255)

                                                gcagcaATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGATCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTgcgc                                                                                                >  2:182757/1‑151 (MQ=255)

                                                 cagcaATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGATCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTgcgcg                                                                                               >  2:80718/1‑151 (MQ=255)

                                                                   aaGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGATCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAAcg                                                                             <  2:533603/151‑1 (MQ=255)

                                                                        ccATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGATCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGAt                                                                        <  2:441820/151‑1 (MQ=255)

                                                                          aTTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGATCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGAtt                                                                       >  1:542211/1‑150 (MQ=255)

                                                                          aTTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGATCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTa                                                                      <  1:286880/151‑1 (MQ=255)

                                                                               aaGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGATCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCa                                                                 <  2:201314/151‑1 (MQ=255)

                                                                                   aggcagCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGATCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACg                                                               >  2:502017/1‑149 (MQ=255)

                                                                                   aggcagCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGATCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAg                                                             <  1:400039/151‑1 (MQ=255)

                                                                                            ccccAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGATCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTAtcgct                                                    <  2:565183/151‑1 (MQ=255)

                                                                                              ccagccagATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGATCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTAtcgctcg                                                  >  2:165937/1‑151 (MQ=255)

                                                                                                agccagATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGATCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATcgctcgct                                                >  2:458160/1‑151 (MQ=255)

                                                                                                         gAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGATCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTg                                       >  2:260236/1‑151 (MQ=255)

                                                                                                             gcCATCAGAGGCAAACGCTTTATCAGTTGATGGACGATCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGttt                                   >  2:406960/1‑151 (MQ=255)

                                                                                                              ccATCAGAGGCAAACGCTTTATCAGTTGATGGACGATCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGtttt                                  <  2:344080/151‑1 (MQ=255)

                                                                                                              ccATCAGAGGCAAACGCTTTATCAGTTGATGGACGATCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGtttt                                  <  1:362965/151‑1 (MQ=255)

                                                                                                                  cAGAGGCAAACGCTTTATCAGTTGATGGACGATCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTgcgc                              <  1:347112/151‑1 (MQ=255)

                                                                                                                    gagGCAAACGCTTTATCAGTTGATGGACGATCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGccc                            >  1:371855/1‑151 (MQ=255)

                                                                                                                       gCAAACGCTTTATCAGTTGATGGACGATCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGcccccc                         <  1:584733/151‑1 (MQ=255)

                                                                                                                         aaaCGCTTTATCAGTTGATGGACGATCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCgg                       >  1:330761/1‑151 (MQ=255)

                                                                                                                                     aGTTGATGGACGATCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGt           >  1:355916/1‑151 (MQ=255)

                                                                                                                                              aCGATCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCg  <  1:368527/151‑1 (MQ=255)

CTGATGAACTACGACAAGCTCGAGTTCGTCGAAACGGTCGAAGAGCTGGCAGCAATGCACAATCTTGAAGTGCCATTTGAAGCAGGCAGCGGCCCCAGCCAGATCGAGCGCCATCAGAGGCAAACGCTTTATCAGTTGATGGACGGTCTGAATACGTTTTACCAACAATCTTTACAACAACCTGTTGCCACGTCTGCGCGCCAGTATCTGGAAAAACGCGGATTAAGCCACGAGGTTATCGCTCGCTTTGCGATTGGTTTTGCGCCCCCCGGCTGGGACAACGTCCTGAAGCG  >  NC_000913/3211326‑3211618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A