Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F50 I2 R1 209 44.2 1360995 98.7% 1343302 150.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,252,977 T→C Y23H (TAC→CAC)  yhaI → DUF805 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,252,9770TC100.0% 62.8 / NA 17Y23H (TAC→CAC) yhaIDUF805 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/6);  total (11/6)

AAGATACGAATAGCATTTACACAATACCCGGAATAGACTTTCGTCACTTTTAATTAAAGGGATGTTTTTATGCAGTGGTATTTGTCCGTATTAAAAAATTATGTTGGTTTCTCTGGTCGCGCACGTCGTAAAGAGTACTGGATGTTTACTCTGATTAACGCCATCGTCGGCGCTATTATCAATGTCATTCAATTGATTTTAGGTCTGGAGCTTCCATATCTGTCTATGCTTTATTTGCTGGCAACCTTCCTTCCCGTCCTTGCGCTTGC  >  NC_000913/3252842‑3253110
                                                                                                                                       |                                                                                                                                     
aagaTACGAATAGCATTTACACAATACCCGGAATAGACTTTCGTCACTTTTAATTAAAGGGATGTTTTTATGCAGTGGTATTTGTCCGTATTAAAAAATTATGTTGGTTTCTCTGGTCGCGCACGTCGTAAAGAGCACTGGATGTTTActc                                                                                                                        >  2:383694/1‑151 (MQ=255)
     aCGAATAGCATTTACACAATACCCGGAATAGACTTTCGTCACTTTTAATTAAAGGGATGTTTTTATGCAGTGGTATTTGTCCGTATTAAAAAATTATGTTGGTTTCTCTGGTCGCGCACGTCGTAAAGAGCACTGGATGTTTACTCTGAtt                                                                                                                   >  2:628290/1‑151 (MQ=255)
            gCATTTACACAATACCCGGAATAGACTTTCGTCACTTTTAATTAAAGGGATGTTTTTATGCAGTGGTATTTGTCCGTATTAAAAAATTATGTTGGTTTCTCTGGTCGCGCACGTCGTAAAGAGCACTGGATGTTTACTCTGATTAACGcc                                                                                                             >  2:76597/1‑150 (MQ=255)
             cATTTACACAATACCCGGAATAGACTTTCGTCACTTTTAATTAAAGGGATGTTTTTATGCAGTGGTATTTGTCCGTATTAAAAAATTATGTTGGTTTCTCTGGTCGCGCACGTCGTAAAGAGCACTGGATGTTTACTCTGATTAACGCCAt                                                                                                           <  2:63318/151‑1 (MQ=255)
                    acaATACCCGGAATAGACTTTCGTCACTTTTAATTAAAGGGATGTTTTTATGCAGTGGTATTTGTCCGTATTAAAAAATTATGTTGGTTTCTCTGGTCGCGCACGTCGTAAAGAGCACTGGATGTTTACTCTGATTAACGCCATCGTCGgc                                                                                                    >  2:649106/1‑151 (MQ=255)
                       aTACCCGGAATAGACTTTCGTCACTTTTAATTAAAGGGATGTTTTTATGCAGTGGTATTTGTCCGTATTAAAAAATTATGTTGGTTTCTCTGGTCGCGCACGTCGTAAAGAGCACTGGATGTTTACTCTGATTAACGCCATCGTCGGCGCt                                                                                                 >  1:491324/1‑151 (MQ=255)
                            cGGAATAGACTTTCGTCACTTTTAATTAAAGGGATGTTTTTATGCAGTGGTATTTGTCCGTATTAAAAAATTATGTTGGTTTCTCTGGTCGCGCACGTCGTAAAGAGCACTGGATGTTTACTCTGATTAACGCCATCGTCGGCGCtattat                                                                                            <  1:62705/151‑1 (MQ=255)
                                    aCTTTCGTCACTTTTAATTAAAGGGATGTTTTTATGCAGTGGTATTTGTCCGTATTAAAAAATTATGTTGGTTTCTCTGGTCGCGCACGTCGTAAAGAGCACTGGATGTTTACTCTGATTAACGCCATCGTCGGCGCTATTATCAATgac                                                                                     >  1:237235/1‑148 (MQ=255)
                                            cACTTTTAATTAAAGGGATGTTTTTATGCAGTGGTATTTGTCCGTATTAAAAAATTATGTTGGTTTCTCTGGTCGCGCACGTCGTAAAGAGCACTGGATGTTTACTCTGATTAACGCCATCGTCGGCGCTATTATCAATGTCATTCAATTg                                                                            <  2:568478/151‑1 (MQ=255)
                                                                        cAGTGGTATTTGTCCGTATTAAAAAATTATGTTGGTTTCTCTGGTCGCGCACGTCGTAAAGAGCACTGGATGTTTACTCTGATTAACGCCATCGTCGGCGCTATTATCAATGTCATTCAATTGATTTTAGGTCTGGAGCTTCCATATCTGt                                                >  1:629203/1‑151 (MQ=255)
                                                                           tGGTATTTGTCCGTATTAAAAAATTATGTTGGTTTCTCTGGTCGCGCACGTCGTAAAGAGCACTGGATGTTTACTCTGATTAACGCCATCGTCGGCGCTATTATCAATGTCATTCAATTGATTTTAGGTCTGGAGCTTCCATATCTGTCt                                              >  2:620580/1‑150 (MQ=255)
                                                                               aTTTGTCCGTATTAAAAAATTATGTTGGTTTCTCTGGTCGCGCACGTCGTAAAGAGCACTGGATGTTTACTCTGATTAACGCCATCGTCGGCGCTATTATCAATGTCATTCAATTGATTTTAGGTCTGGAGCTTCCATATCTGTCTATGc                                          <  1:296378/150‑1 (MQ=255)
                                                                                   gTCCGTATTAAAAAATTATGTTGGTTTCTCTGGTCGCGCACGTCGTAAAGAGCACTGGATGTTTACTCTGATTAACGCCATCGTCGGCGCTATTATCAATGTCATTCAATTGATTTTAGGTCTGGAGCTTCCATATCTGTCTATGCTTTAt                                     >  2:670073/1‑151 (MQ=255)
                                                                                         aTTAAAAAATTATGTTGGTTTCTCTGGTCGCGCACGTCGTAAAGAGCACTGGATGTTTACTCTGATTAACGCCATCGTCGGCGCTATTATCAATGTCATTCAATTGATTTTAGGTCTGGAGCTTCCATATCTGTCTATGCTTTATTTGCTg                               <  1:658998/151‑1 (MQ=255)
                                                                                          ttAAAAAATTATGTTGGTTTCTCTGGTCGCGCACGTCGTAAAGAGCACTGGATGTTTACTCTGATTAACGCCATCGTCGGCGCTATTATCAATGTCATTCAATTGATTTTAGGTCTGGAGCTTCCATATCTGTCTATGCTTTATTTGCTgg                              >  1:260568/1‑151 (MQ=255)
                                                                                                          gTTTCTCTGGTCGCGCACGTCGTAAAGAGCACTGGATGTTTACTCTGATTAACGCCATCGTCGGCGCTATTATCAATGTCATTCAATTGATTTTAGGTCTGGAGCTTCCATATCTGTCTATGCTTTATTTGCTGGCAACCTTCCTTCCCGt              <  1:620599/151‑1 (MQ=255)
                                                                                                                      gcgcACGTCGTAAAGAGCACTGGATGTTTACTCTGATTAACGCCATCGTCGGCGCTATTATCAATGTCATTCAATTGATTTTAGGTCTGGAGCTTCCATATCTGTCTATGCTTTATTTGCTGGCAACCTTCCTTCCCGTCCTTGCGCTTGc  >  1:297084/1‑151 (MQ=255)
                                                                                                                                       |                                                                                                                                     
AAGATACGAATAGCATTTACACAATACCCGGAATAGACTTTCGTCACTTTTAATTAAAGGGATGTTTTTATGCAGTGGTATTTGTCCGTATTAAAAAATTATGTTGGTTTCTCTGGTCGCGCACGTCGTAAAGAGTACTGGATGTTTACTCTGATTAACGCCATCGTCGGCGCTATTATCAATGTCATTCAATTGATTTTAGGTCTGGAGCTTCCATATCTGTCTATGCTTTATTTGCTGGCAACCTTCCTTCCCGTCCTTGCGCTTGC  >  NC_000913/3252842‑3253110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A