Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F50 I2 R1 209 44.2 1360995 98.7% 1343302 150.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,362,012 A→G intergenic (+72/‑100) gltF → / → yhcA periplasmic protein/putative periplasmic chaperone protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,362,0120AG100.0% 179.3 / NA 48intergenic (+72/‑100)gltF/yhcAperiplasmic protein/putative periplasmic chaperone protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/22);  total (26/22)

GACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGACATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCATTATTACAGGAGTTTTAATTTAAATGCTCAGACACATTACATTCACTGTATTTATAACAACATCAATGA  >  NC_000913/3361866‑3362157
                                                                                                                                                  |                                                                                                                                                 
gACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCAt                                                                                                                                                <  2:70700/150‑1 (MQ=255)
gACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATg                                                                                                                                               <  1:449605/151‑1 (MQ=255)
gACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATg                                                                                                                                               <  1:614021/151‑1 (MQ=255)
   aaTACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGgtc                                                                                                                                            >  2:204986/1‑151 (MQ=255)
   aaTACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGgtc                                                                                                                                            <  1:581793/151‑1 (MQ=255)
     tACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGgtctg                                                                                                                                          >  2:104570/1‑151 (MQ=255)
       cTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGgtctgtc                                                                                                                                        >  2:44347/1‑151 (MQ=255)
        tATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGgtctgtct                                                                                                                                       >  1:21491/1‑151 (MQ=255)
          ttCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCtt                                                                                                                                      >  2:629421/1‑150 (MQ=255)
           tCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTa                                                                                                                                     <  1:204991/150‑1 (MQ=255)
                   cTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAAtctc                                                                                                                            >  2:3120/1‑151 (MQ=255)
                       tgaAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCg                                                                                                                        >  2:320482/1‑151 (MQ=255)
                         aaaCGATCAAACTGGATGGTAACGTCACTATCCGCGTTCAGTATTTATAAGAAATTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAACTAAAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATc                                                                                                                         <  2:420144/148‑1 (MQ=255)
                          aaCGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCa                                                                                                                      >  1:588508/1‑150 (MQ=255)
                                       gATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACa                                                                                                        <  1:223232/151‑1 (MQ=255)
                                             aaCGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACatatat                                                                                                   <  1:573978/150‑1 (MQ=255)
                                              aCGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTa                                                                                                 <  1:478460/151‑1 (MQ=255)
                                                 tCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAAc                                                                                               >  1:603881/1‑150 (MQ=255)
                                                  cACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACaa                                                                                             <  2:219913/151‑1 (MQ=255)
                                                  cACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACaa                                                                                             <  1:397256/151‑1 (MQ=255)
                                                  cACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACa                                                                                              >  2:301846/1‑150 (MQ=255)
                                                         aGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGttt                                                                                      <  2:426237/151‑1 (MQ=255)
                                                           cGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGATCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCt                                                                                    <  2:126112/151‑1 (MQ=255)
                                                                aGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGtt                                                                               <  1:197556/151‑1 (MQ=255)
                                                                   aTTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGttatt                                                                            >  1:614864/1‑151 (MQ=255)
                                                                      tataAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGttattta                                                                          <  2:163339/150‑1 (MQ=255)
                                                                         aaGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCat                                                                      >  2:212820/1‑151 (MQ=255)
                                                                            aaCTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCattat                                                                   >  2:234821/1‑151 (MQ=255)
                                                                                tGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCATTATTAc                                                                >  2:317621/1‑150 (MQ=255)
                                                                                tGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCATTATTAc                                                                >  1:531686/1‑150 (MQ=255)
                                                                                 gTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCATTATTACAg                                                              <  1:657501/151‑1 (MQ=255)
                                                                                    aTACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCATTATTACAGGAg                                                           <  2:616172/151‑1 (MQ=255)
                                                                                    aTACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCATTATTACAGGAg                                                           <  1:606279/151‑1 (MQ=255)
                                                                                     tACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCATTATTACAGGAGt                                                          >  1:636051/1‑151 (MQ=255)
                                                                                        aGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCATTATTACAGGAGtttt                                                       >  1:261969/1‑151 (MQ=255)
                                                                                               atttttaCGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCATTATTACAGGAGTtttaattta                                                 >  2:283463/1‑150 (MQ=255)
                                                                                                       gCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCATTATTACAGGAGTTTTAATTTAAATGCTc                                          >  2:72849/1‑149 (MQ=255)
                                                                                                       gCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCATTATTACAGGAGTTTTAATTTAAATGCTCAg                                        <  1:92678/151‑1 (MQ=255)
                                                                                                           tAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCATTATTACAGGAGTTTTAATTTAAATGCTCAGacac                                    >  2:109111/1‑151 (MQ=255)
                                                                                                              ccAGTTAGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCATTATTACAGGAGTTTTAATTTAAATGCTCAGACacatt                                 >  2:81966/1‑151 (MQ=255)
                                                                                                                    aGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCATTATTACAGGAGTTTTAATTTAAATGCTCAGACACATTACATTc                           <  1:88239/151‑1 (MQ=255)
                                                                                                                    aGCTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCATTATTACAGGAGTTTTAATTTAAATGCTCAGACACATTACATTc                           >  1:461851/1‑151 (MQ=255)
                                                                                                                      cTATAACTCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCATTATTACAGGAGTTTTAATTTAAATGCTCAGACACATTACATTCAc                         >  2:365152/1‑151 (MQ=255)
                                                                                                                             tCGGACAGATTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCATTATTACAGGAGTTTTAATTTAAATGCTCAGACACATTACATTCACTGTAttt                  >  1:379810/1‑151 (MQ=255)
                                                                                                                                     aTTATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTACTGCGTTATTTATCATTATTACAGGAGTTTTAATTTAAATGCTCAGACACATTACATTCACTGTATTTATaacaac          <  1:611788/151‑1 (MQ=255)
                                                                                                                                       tATGCCCTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCATTATTACAGGAGTTTTAATTTAAATGCTCAGACACATTACATTCACTGTATTTATAACAACAt        >  2:440788/1‑151 (MQ=255)
                                                                                                                                            ccTATGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCATTATTACAGGAGTTTTAATTTAAATGCTCAGACACATTACATTCACTGTATTTATAACAACATCAATg   >  2:419868/1‑151 (MQ=255)
                                                                                                                                               aTGGCATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCATTATTACAGGAGTTTTAATTTAAATGCTCAGACACATTACATTCACTGTATTTATAACAACATCAATGa  <  2:196794/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GACAATACTATTCTTGGTTCTGATGAAACGATCAAACTGGATGGTAACGTCACTATCAGCGTTCAGTATTTATAAGAACTTGTAATACAGTTTTTATTTTTACGCATTAACCAGTTAGCTATAACTCGGACAGATTATGCCCTATGACATGGTCTGTCTTAATCAATCTCATCGCAAACAGTGCATAACATATATTAACAATATGTTTCTGCGTTATTTATCATTATTACAGGAGTTTTAATTTAAATGCTCAGACACATTACATTCACTGTATTTATAACAACATCAATGA  >  NC_000913/3361866‑3362157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A