Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,572,038	T→C	T489A (ACC→GCC)	glgB ←	1,4‑alpha‑glucan branching enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,572,038	0	T	C	100.0%	181.9 / NA	51	T489A (ACC→GCC)	glgB	1,4‑alpha‑glucan branching enzyme
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (26/25); total (26/25)

CGGTCGAGAATCGATTTTTTACCGTGGACCACTTCATCATGCGACAACGGCAGGACGAAGTTTTCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGTGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTCACCGCACCGGAAACCTGCTCACCAAGAATACGGTTGGTATT  >  NC_000913/3571890‑3572182

cGGTCGAGAATCGATTTTTTACCGTGGACCACTTCATCATGCGACAACGGCAGGACGAAGTTTTCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCTAGATTCATGTAGTCCAGGgcgt                                                                                                                                                >  2:33743/1‑151 (MQ=255)

  gTCGAGAATCGATTTTTTACCGTGGACCACTTCATCATGCGACAACGGCAGGACGAAGTTTTCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGgcgt                                                                                                                                                >  1:404351/1‑149 (MQ=255)

    cGAGAATCGATTTTTTACCGTGGACCACTTCATCATGCGACAACGGCAGGACGAAGTTTTCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCAt                                                                                                                                             <  2:231935/150‑1 (MQ=255)

    cGAGAATCGATTTTTTACCGTGGACCACTTCATCATGCGACAACGGCAGGACGAAGTTTTCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATg                                                                                                                                            <  2:595286/151‑1 (MQ=255)

      agaATCGATTTTTTACCGTGGACCACTTCATCATGCGACAACGGCAGGACGAAGTTTTCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGc                                                                                                                                           >  2:142453/1‑150 (MQ=255)

            gATTTTTTACCGTGGACCACTTCATCATGCGACAACGGCAGGACGAAGTTTTCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAg                                                                                                                                     <  2:391684/150‑1 (MQ=255)

             aTTTTTTACCGTGGACCACTTCATCATGCGACAACGGCAGGACGAAGTTTTCAGTGTAGTTGTAGAAAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGcc                                                                                                                                   >  1:555132/1‑151 (MQ=255)

                 tttACCGTGGACCACTTCATCATGCGACAACGGCAGGACGAAGTTTTCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAgg                                                                                                                               >  2:516434/1‑151 (MQ=255)

                       gTGGACCACTTCATCATGCGACAACGGCAGGACGAAGTTTTCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCAc                                                                                                                         >  1:223824/1‑151 (MQ=255)

                         ggACCACTTCATCATGCGACAACGGCAGGACGAAGTTTTCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACt                                                                                                                        <  1:45716/150‑1 (MQ=255)

                             cACTTCATCATGCGACAACGGCAGGACGAAGTTTTCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTAc                                                                                                                   >  2:644572/1‑151 (MQ=255)

                                 tcatcaTGCGACAACGGCAGGACGAAGTTTTCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTAcc                                                                                                                  >  1:413022/1‑148 (MQ=255)

                                  catcatGCGACAACGGCAGGACGAAGTTTTCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGa                                                                                                               <  2:362466/150‑1 (MQ=255)

                                     catGCGACAACGGCAGGACGAAGTTTTCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGcc                                                                                                           >  2:291603/1‑151 (MQ=255)

                                       tGCGACAACGGCAGGACGAAGTTTTCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGccc                                                                                                          >  1:48060/1‑150 (MQ=255)

                                          gACAACGGCAGGACGAAGTTTTCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGAc                                                                                                      <  2:481895/151‑1 (MQ=255)

                                                  cAGGACGAAGTTTTCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCat                                                                                               >  1:295645/1‑150 (MQ=255)

                                                    ggACGAAGTTTTCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATc                                                                                            <  1:191962/151‑1 (MQ=255)

                                                        gAAGTTTTCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGc                                                                                        <  1:680249/151‑1 (MQ=255)

                                                          aGTTTTCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCgg                                                                                      <  2:174056/151‑1 (MQ=255)

                                                            ttttCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGAc                                                                                    >  2:34185/1‑151 (MQ=255)

                                                              ttCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACg                                                                                   <  2:596768/150‑1 (MQ=255)

                                                                           tAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAgg                                                                       <  2:500775/149‑1 (MQ=255)

                                                                                aaTCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCg                                                                <  2:522021/151‑1 (MQ=255)

                                                                                      gAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGAc                                                          >  2:439342/1‑151 (MQ=255)

                                                                                       aaGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGAct                                                         <  1:566235/151‑1 (MQ=255)

                                                                                       aaGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGAct                                                         >  2:562843/1‑151 (MQ=255)

                                                                                             aGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGActcctc                                                     >  1:341322/1‑149 (MQ=255)

                                                                                             aGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGActcctc                                                     >  2:44568/1‑149 (MQ=255)

                                                                                              gTTTAGCGTGATGATACTGACCATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGc                                                  <  2:23695/151‑1 (MQ=255)

                                                                                                  aTCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCAtt                                              >  2:1727/1‑151 (MQ=255)

                                                                                                        atgatACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTcacc                                        <  2:133436/151‑1 (MQ=255)

                                                                                                        atgatACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCAGAGGTTCTACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTcacc                                        >  2:633869/1‑151 (MQ=255)

                                                                                                          gatACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTcaccg                                       <  1:90824/150‑1 (MQ=255)

                                                                                                            tACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTcactgcac                                    >  2:146210/1‑151 (MQ=255)

                                                                                                              cTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTcaccgcaccg                                  >  1:505185/1‑151 (MQ=255)

                                                                                                                gACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTCACCGCACCgg                                 >  1:147895/1‑150 (MQ=255)

                                                                                                                   gATAAACCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTCACCGCACCGGAAAc                             >  2:305574/1‑151 (MQ=255)

                                                                                                                      aaaCCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTCACCGCACCGGAAACCt                           <  2:231643/150‑1 (MQ=255)

                                                                                                                        aCCGGGTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTCACCGCACCGGAAACCTGCt                        >  1:171170/1‑151 (MQ=255)

                                                                                                                           gggTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTCACCGCACCGGAAACCTGCTCAc                     <  1:432331/151‑1 (MQ=255)

                                                                                                                           gggTCGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTCACCGCACCGGAAACCTGCTCAc                     <  2:287756/151‑1 (MQ=255)

                                                                                                                               cGAGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTCACCGCACCGGAAACCTGCTCACCa                   >  2:731/1‑149 (MQ=255)

                                                                                                                                 aGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTCACCGCACCGGAAACCTGCTCACCAAGaa               <  2:479753/151‑1 (MQ=255)

                                                                                                                                 aGCTTCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTCACCGCACCGGAAACCTGCTCACCAAGaa               <  2:465293/151‑1 (MQ=255)

                                                                                                                                     tCATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTCACCGCACCGGAAACCTGCTCACCAAGAATAc            <  1:594840/150‑1 (MQ=255)

                                                                                                                                      cATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTCACCGCACCGGAAACCTGCTCACCAAGAATACgg          <  1:464394/151‑1 (MQ=255)

                                                                                                                                      cATGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTCACCGCACCGGAAACCTGCTCACCAAGAATACg           <  2:56847/150‑1 (MQ=255)

                                                                                                                                        tGTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTCACCGCACCGGAAACCTGCTCACCAAGAATACGGtt        >  1:103934/1‑151 (MQ=255)

                                                                                                                                         gTAGTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTCACCGCACCGGAAACCTGCTCACCAAGAATACGGTTg       <  1:150823/151‑1 (MQ=255)

                                                                                                                                            gTCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTCACCGCACCGGAAACCTGCTCACCAAGAATACGGTTGGt     >  1:11196/1‑150 (MQ=255)

                                                                                                                                             tCCAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTCACCGCACCGGAAACCTGCTCACCAAGAATACGGTTGGTAt   >  1:13267/1‑151 (MQ=255)

                                                                                                                                              ccAGGGCGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTCACCGCACCGGAAACCTGCTCACCAAGAATACGGTTGGTAtt  >  1:633115/1‑151 (MQ=255)

CGGTCGAGAATCGATTTTTTACCGTGGACCACTTCATCATGCGACAACGGCAGGACGAAGTTTTCAGTGTAGTTGTAGAGAATCCCGAAGGTCAGTTTATCGTGATGATACTGACGATAAACCGGGTCGAGCTTCATGTAGTCCAGGGTGTCATGCATCCAGCCGAGGTTCCACTTGTACCAGAAGCCCAGACCGCCCATATCCTGCGGACGAGAAACGCCAGGGAAATCGGTAGACTCCTCAGCCATTGTCACCGCACCGGAAACCTGCTCACCAAGAATACGGTTGGTATT  >  NC_000913/3571890‑3572182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A