Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,575,146	T→C	S26P (TCC→CCC)	yhgN →	UPF0056 family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,575,146	0	T	C	100.0%	137.1 / NA	37	S26P (TCC→CCC)	yhgN	UPF0056 family inner membrane protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (20/17); total (20/17)

TCAGTAATGCGACTTATCCTGCTTTGTTAGCACGCAGAAAGTCCGCGGCAATTATCAGGGAATTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCAT  >  NC_000913/3575001‑3575291

tCAGTAATGCGACTTATCCTGCTTTGTTAGCACGCAGAAAGTCCGCGGCAATTATCAGGGAATTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGt                                                                                                                                               >  1:88505/1‑150 (MQ=255)

 cAGTAATGCGACTTATCCTGCTTTGTTAGCACGCAGAAAGTCCGCGGCAATTATCAGGGAATTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTAc                                                                                                                                             >  2:286049/1‑151 (MQ=255)

   gTAATGCGACTTATCCTGCTTTGTTAGCACGCAGAAAGTCCGCGGCAATTATCAGGGAATTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTg                                                                                                                                           <  2:201882/151‑1 (MQ=255)

   gTAATGCGACTTATCCTGCTTTGTTAGCACGCAGAAAGTCCGCGGCAATTATCAGGGAATTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTg                                                                                                                                           <  1:574797/151‑1 (MQ=255)

     aaTGCGACTTATCCTGCTTTGTTAGCACGCAGAAAGTCCGCGGCAATTATCAGGGAATTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGTTTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGa                                                                                                                                          >  2:209329/1‑150 (MQ=255)

      aTGCGACTTATCCTGCTTTGTTAGCACGCAGAAAGTCCGCGGCAATTATCAGGGAATTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGaa                                                                                                                                         >  2:568602/1‑150 (MQ=255)

          gACTTATCCTGCTTTGTTAGCACGCAGAAAGTCCGCGGCAATTATCAGGGAATTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACAt                                                                                                                                     >  1:427559/1‑150 (MQ=255)

               aTCCTGCTTTGTTAGCACGCAGAAAGTCCGCGGCAATTATCAGGGAATTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGa                                                                                                                                <  2:318203/150‑1 (MQ=255)

                    gCTTTGTTAGCACGCAGAAAGTCCGCGGCAATTATCAGGGAATTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGaa                                                                                                                          <  1:96587/151‑1 (MQ=255)

                       ttGTTAGCACGCAGAAAGTCCGCGGCAATTATCAGGGAATTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGaaa                                                                                                                         >  2:285739/1‑149 (MQ=255)

                         gTTAGCACGCAGAAAGTCCGCGGCAATTATCAGGGAATTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGAc                                                                                                                     <  1:622116/151‑1 (MQ=255)

                                   aGAAAGTCCGCGGCAATTATCAGGGAATTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAAt                                                                                                           >  1:63960/1‑151 (MQ=255)

                                           cgcgGCAATTATCAGGGAATTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGc                                                                                                   <  2:276238/151‑1 (MQ=255)

                                               gCAATTATCAGGGAATTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCg                                                                                                  >  1:409271/1‑148 (MQ=255)

                                                             aTTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGctc                                                                                 >  1:61867/1‑151 (MQ=255)

                                                                 gAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCt                                                                             <  1:650363/151‑1 (MQ=255)

                                                                 gAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCt                                                                             <  2:635011/151‑1 (MQ=255)

                                                                 gAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGACCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCt                                                                             <  1:495698/151‑1 (MQ=255)

                                                                  aGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTg                                                                            <  1:120230/151‑1 (MQ=255)

                                                                     tATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGTGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTg                                                                         >  1:516869/1‑151 (MQ=255)

                                                                            gaaATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTgg                                                                  >  2:670050/1‑151 (MQ=255)

                                                                                    ttCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCt                                                           <  1:253831/150‑1 (MQ=255)

                                                                                    ttCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTg                                                          >  2:550064/1‑151 (MQ=255)

                                                                                     tCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGt                                                         >  2:143239/1‑151 (MQ=255)

                                                                                          agcagTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCg                                                    >  1:149819/1‑151 (MQ=255)

                                                                                            cagTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCggg                                                  <  1:47931/151‑1 (MQ=255)

                                                                                               ttttATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTg                                                >  1:582168/1‑150 (MQ=255)

                                                                                                   aTTGATCCTGATTATGGATCCGCTAGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGaaa                                           <  1:6329/151‑1 (MQ=255)

                                                                                                       aTCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTc                                       >  2:104538/1‑151 (MQ=255)

                                                                                                         ccTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTg                                     >  2:92279/1‑151 (MQ=255)

                                                                                                               tATGGATCCGCTCGGAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCAttt                               >  2:16473/1‑151 (MQ=255)

                                                                                                                            ggAAACCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAg                  <  2:531028/151‑1 (MQ=255)

                                                                                                                              aaaCCTACCTATTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGc                 >  1:215338/1‑150 (MQ=255)

                                                                                                                                        aTTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGtc       <  2:225047/150‑1 (MQ=255)

                                                                                                                                        aTTTTCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGt        <  2:304622/149‑1 (MQ=255)

                                                                                                                                           ttCATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTcc    >  2:539829/1‑150 (MQ=255)

                                                                                                                                             cATGCCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCAt  <  1:188289/150‑1 (MQ=255)

TCAGTAATGCGACTTATCCTGCTTTGTTAGCACGCAGAAAGTCCGCGGCAATTATCAGGGAATTTGAGTTATGAATGAAATCATTTCTGCAGCAGTTTTATTGATCCTGATTATGGATCCGCTCGGAAACCTACCTATTTTCATGTCCGTACTGAAACATACTGAACCGAAAAGACGGCGGGCAATCATGGTGCGAGAGTTGCTTATTGCTCTCCTGGTGATGCTGGTGTTCCTGTTTGCGGGTGAGAAAATTCTGGCATTTCTTAGCCTACGAGCAGAAACCGTCTCCAT  >  NC_000913/3575001‑3575291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A