Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,696,621	A→G	H55R (CAC→CGC)	bcsE →	cellulose production protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,696,621	0	A	G	100.0%	145.4 / NA	44	H55R (CAC→CGC)	bcsE	cellulose production protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (20/24); total (20/24)

CCTGTATTCTCTATCGGTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGTCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACACGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCTTTCAATTAGATGATTCTCAAGGGCCGGAAAAAATAAAATTATTTTCAATGCTAAATCATGAAAAAGGTCTATACTATTTGACCCGTGATTTGCAGTGTTCTATTGA  >  NC_000913/3696476‑3696768

ccTGTATTCTCTATCGGTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGTCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACGCGTcg                                                                                                                                                >  2:375393/1‑151 (MQ=255)

      ttCTCTATCGGTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGTCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACGCGTCGCGGTc                                                                                                                                           >  2:483249/1‑150 (MQ=255)

                           ttATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGTCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACGCGTCGCGGTCATTAGCATGGACAGCGATCCgg                                                                                                                     >  1:353176/1‑151 (MQ=255)

                             aTGGGATGAGCTGCGACATATGCCAGCAGGCGGCGTCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACGCGTCGCGGTCATTAGCATGGACAGCGATCCGGCg                                                                                                                   >  2:123718/1‑151 (MQ=255)

                                 gATGAGCTGCGACATATGCCAGCAGGCGGCGTCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACGCGTCGCGGTCATTAGCATGGACAGCGATCCGGCGaaaa                                                                                                               <  2:138259/151‑1 (MQ=255)

                                  aTGAGCTGCGACATATGCCAGCAGGCGGCGTCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACGCGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAAt                                                                                                              >  1:228543/1‑151 (MQ=255)

                                         gCGACATATGCCAGCAGGCGGCGTCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACGCGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCttt                                                                                                          <  2:473844/148‑1 (MQ=255)

                                         gCGACATATGCCAGCAGGCGGCGTCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACGCGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCTTTc                                                                                                         <  2:228538/149‑1 (MQ=255)

                                           gACATATGCCAGCAGGCGGCGTCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACGCGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCTTTCAAtt                                                                                                     <  1:188128/151‑1 (MQ=255)

                                              atatGCCAGCAGGCGGCGTCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACGCGTCGCGGTCATTAGCATGGAAAGCGATCCGGCGAAAATCTTTCAATTa                                                                                                    <  2:558873/149‑1 (MQ=255)

                                               tatGCCAGCAGGCGGCGTCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACGCGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCTTTCAAtt                                                                                                     >  2:30276/1‑147 (MQ=255)

                                                atGCCAGCAGGCGGCGTCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACGCGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCTTTCAATTAgatg                                                                                                <  2:621056/151‑1 (MQ=255)

                                                     agcagGCGGCGTCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACGCGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCTTTCAATTAGATGATtct                                                                                           >  2:256751/1‑151 (MQ=255)

                                                      gcagGCGGCGTCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACGCGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCTTTCAATTAGATGATtctc                                                                                          <  1:414384/151‑1 (MQ=255)

                                                        agGCGGCGTCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACGCGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCTTTCAATTAGATGATTCTCaa                                                                                        <  2:518241/151‑1 (MQ=255)

                                                               gTCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACGCGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCTTTCAATTAGATGATTCTCAAGGGCCgg                                                                                 >  1:604609/1‑151 (MQ=255)

                                                                 cTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACGCGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCTTTCAATTAGATGATTCTCAAGGGCCGGa                                                                                >  2:247425/1‑150 (MQ=255)

                                                                  tggtggTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACGCGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCTTTCAATTAGATGATTCTCAAGGGCCGGaa                                                                               >  2:22525/1‑150 (MQ=255)

                                                                   ggtggtTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACGCGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCTTTCAATTAGATGATTCTCAAGGGCCGGaaa                                                                              <  1:144396/150‑1 (MQ=255)

                                                                   ggtggtTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACGCGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCTTTCAATTAGATGATTCTCAAGGGCCGGa                                                                                <  2:511631/148‑1 (MQ=255)

                                                                    gtgctgtAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAAGCAAACAATTGCATCCCAGGCTGAAACCGCACGCGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCTTTCAATTAGATGATTCTCAAAGGCCGGaaaaa                                                                            <  2:126870/145‑1 (MQ=255)

                                                                             cGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACGCGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCTTTCAATTAGATGATTCTCAAGGGCCGGAAAAAATAAAatta                                                                   <  2:395900/151‑1 (MQ=255)

                                                                               tCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACGCGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCTTTCAATTAGATGATTCTCAAGGGCCGGAAAAAATAAAattatt                                                                 >  2:306350/1‑151 (MQ=255)

                                                                                  aTCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACGCGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCTTTCAATTAGATGATTCTCAAGGGCCGGAAAAAATAAAATTATTTTc                                                              <  1:61098/151‑1 (MQ=255)

                                                                                        aTGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACGCGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCTTTCAATTAGATGATTCTCAAGGGCCGGAAAAAATAAAATTATTTTCAATGCt                                                        <  1:179239/151‑1 (MQ=255)

                                                                                           aaGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACGCGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCTTTCAATTAGATGATTCTCAAGGGCCGGAAAAAATAAAATTATTTTCAATGCTaa                                                      >  1:600482/1‑150 (MQ=255)

                                                                                           aaGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACGCGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCTTTCAATTAGATGATTCTCAAGGGCCGGAAAAAATAAAATTATTTTCAATGCTaa                                                      >  1:599962/1‑150 (MQ=255)

                                                                                              aTGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACGCGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCTTTCAATTAGATGATTCTCAAGGGCCGGAAAAAATAAAATTATTTTCAATGCTAAATCa                                                  <  1:11612/151‑1 (MQ=255)

                                                                                                   aTCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACGCGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCTTTCAATTAGATGATTCTCAAGGGCCGGAAAAAATAAAATTATTTTCAATGCTAAATCATGaaa                                             <  1:505479/151‑1 (MQ=255)

                                                                                                     cAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACGCGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCTTTCAATTAGATGATTCTCAAGGGCCGGAAAAAATAAAATTATTTTCAATGCTAAATCATGaaaa                                            >  2:249657/1‑150 (MQ=255)

                                                                                                      aGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACGCGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCTTTCAATTAGATGATTCTCAAGGGCCGGAAAAAATAAAATTATTTTCAATGCTAAATCATGaaaa                                            >  2:164036/1‑149 (MQ=255)

                                                                                                       gtatGGCGAATCAAACAATTGCACCCCAGGCTGAAACCGCACGCGTCGCGGTCATGAGCACGGACAGCGATCCAGCGAAAATCTTTCTATTAGATGATTCTCAAGGGCCGGAACAAATAAAATCATTTTCACTGCTAAATCATGAAAAAgg                                         >  2:435553/4‑151 (MQ=255)

                                                                                                         cTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACGCGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCTTTCAATTAGATGATTCTCAAGGGCCGGAAAAAATAAAATTATTTTCAATGCTAAATCATGAAAAAGGTc                                       >  2:572276/1‑151 (MQ=255)

                                                                                                             cGAATCAAACAATTGCATCCCAGGCTGAAACCGCACGCGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCTTTCAATTAGATGATTCTCAAGGGCCGGAAAAAATAAAATTATTTTCAATGCTAAATCATGAAAAAGGTCtata                                   <  2:529931/151‑1 (MQ=255)

                                                                                                              gAATCAAACAATTGCATCCCAGGCTGAAACCGCACGCGTCGCGGTCATTAGCATGGACAGCGATCCGGCTAAAATCTTTCAATTAGATGATTCTCAAGGGCCGGAAAAAATAAAATTATTTTCAATGCTAAATCATGAAAAAGGTCTATAc                                  <  1:265657/151‑1 (MQ=255)

                                                                                                                           gCATCCCAGGCTGAAACCGCACGCGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCTTTCAATTAGATGATTCTCAAGGGCCGGAAAAAATAAAATTATTTTCAATGCTAAATCATGAAAAAGGTCTATACTATTTGACCCGTg                     <  1:123721/151‑1 (MQ=255)

                                                                                                                           gCATCCCAGGCTGAAACCGCACGCGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCTTTCAATTAGATGATTCTCAAGGGCCGGAAAAAATAAAATTATTTTCAATGCTAAATCATGAAAAAGGTCTATACTATTTGACCCGTg                     >  2:367445/1‑151 (MQ=255)

                                                                                                                             aTCCCAGGCTGAAACCGCACGCGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCTTTCAATTAGATGATTCTCAAGGGCCGGAAAAAATAAAATTATTTTCAATGCTAAATCATGAAAAAGGTCTATACTATTTGACCCGTGAt                   >  1:217702/1‑151 (MQ=255)

                                                                                                                               cccAGGCTGAAACCGCACGCGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCTTTCAATTAGATGATTCTCAAGGGCCGGAAAAAATAAAATTATTTTCAATGCTAAATCATGAAAAAGGTCTATACTATTTGACCCGTGAttt                 <  1:191403/151‑1 (MQ=255)

                                                                                                                               cccAGGCTGAAACCGCACGCGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCTTTCAATTAGATGATTCTCAAGGGCCGGAAAAAATAAAATTATTTTCAATGCTAAATCATGAAAAAGGTCTATACTATTTGACCCGTGAtt                  <  1:143107/150‑1 (MQ=255)

                                                                                                                                         aaCCGCACGCGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCTTTCAATTAGATGATTCTCAAGGGCCGGAAAAAATAAAATTATTTTCAATGCTAAATCATGAAAAAGGTCTATACTATTTGACCCGTGATTTGCAGTGTTCt       <  2:649537/151‑1 (MQ=255)

                                                                                                                                          aCCGCACGCGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCTTTCAATTAGATGATTCTCAAGGGCCGGAAAAAATAAAATTATTTTCAATGCTAAATCATGAAAAAGGTCTATACTATTTGACCCGTGATTTGCAGTGTTCTa      <  1:343461/151‑1 (MQ=255)

                                                                                                                                           ccGCACGCGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCTTTCAATTAGATGATTCTCAAGGGCCGGAAAAAATAAAATTATTTTCAATGCTAAATCATGAAAAAGGTCTATACTATTTGACCCGTGATTTGCAGTGTTCTAt     <  2:30667/151‑1 (MQ=255)

                                                                                                                                             gCACGCGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCTTTCAATTAGATGATTCTCAAGGGCCGGAAAAAATAAAATTATTTTCAATGCTAAATCATGAAAAAGGTCTATACTATTTGACCCGTGATTTGCAGTGTTCTATTg   >  1:225046/1‑151 (MQ=255)

                                                                                                                                              cacgCGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCTTTCAATTAGATGATTCTCAAGGGCCGGAAAAAATAAAATTATTTTCAATGCTAAATCATGAAAAAGGTCTATACTATTTGACCCGTGATTTGCAGTGTTCTATTg   >  2:386894/1‑150 (MQ=255)

                                                                                                                                              cacgCGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCTTTCAATTAGATGATTCTCAAGGGCCGGAAAAAATAAAATTATTTTCAATGCTAAATCATGAAAAAGGTCTATACTATTTGACCCGTGATTTGCAGTGTTCTATTGa  <  1:133459/151‑1 (MQ=255)

CCTGTATTCTCTATCGGTATCTCATCATTATGGGATGAGCTGCGACATATGCCAGCAGGCGGCGTCTGGTGGTTTAACGTCGATCGCCATGAAGATGCTATCAGTCTGGCGAATCAAACAATTGCATCCCAGGCTGAAACCGCACACGTCGCGGTCATTAGCATGGACAGCGATCCGGCGAAAATCTTTCAATTAGATGATTCTCAAGGGCCGGAAAAAATAAAATTATTTTCAATGCTAAATCATGAAAAAGGTCTATACTATTTGACCCGTGATTTGCAGTGTTCTATTGA  >  NC_000913/3696476‑3696768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A