Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,715,195	T→C	E400E (GAA→GAG)	bisC ←	biotin sulfoxide reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,715,195	0	T	C	100.0%	127.0 / NA	33	E400E (GAA→GAG)	bisC	biotin sulfoxide reductase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (16/17); total (16/17)

CGGTTTTTGCCAGGCACGGATCAGGCGATTGGTATCCTGATGATGAGTAAAGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCGGGAAATGTCGGTTCATACCGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCTTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCACCCGGCAAGCTGCCCTGCATGGAAGAGAGCACCGCAGAACGCCGCGTGGGGTTACCACCATTGGCAAAATGGTAAGAAAGACCAAAACCGCCGCCGG  >  NC_000913/3715051‑3715337

cGGTTTTTGCCAGGCACGGATCAGGCGATTGGTATCCTGATGATGAGTAAAGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCGGGAAATGTCGGTTCATACCGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCCTcaac                                                                                                                                           >  2:129476/1‑150 (MQ=255)

                      aGGCGATTGGTATCCTGATGATGAGTAAAGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCGGGAAATGTCGGTTCATACCGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCCTCAACAATGCGGGCAACAGGGATTTTAt                                                                                                                    >  2:103218/1‑151 (MQ=255)

                       ggCGATTGGTATCCTGATGATGAGTAAAGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCGGGAAATGTCGGTTCATACCGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCCTCAACAATGCGGGCAACAGGGATTTTATc                                                                                                                   <  1:120885/151‑1 (MQ=255)

                         cGATTGGTATCCTGATGATGAGTAAAGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCGGGAAATGTCGGTTCATACCGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCCTCAACAATGCGGGCAACAGGGATTTTATCCa                                                                                                                 <  2:264792/151‑1 (MQ=255)

                             tGGTATCCTGATGATGAGTAAAGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCGGGAAATGTCGGTTCATACCGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCCTCAACAATGCGGGCAACAGGGATTTTATCCACCg                                                                                                              <  1:402031/150‑1 (MQ=255)

                                               tAAAGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCGGGAAATGTCGGTTCATACCGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCCTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCACCCGGCa                                                                                            >  1:647981/1‑150 (MQ=255)

                                                  aGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCGGGAAATGTCGGTTCATACCGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCCTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCACCCGGCAAGCt                                                                                        <  2:103681/151‑1 (MQ=255)

                                                                gcccACCAGATAAAACGAATATCCGGGAAATGTCGGTTCATACCGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCCTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCACCCGGCAAGCTGCCCTGCATGGa                                                                            >  1:18524/1‑149 (MQ=255)

                                                                gcccACCAGATAAAACGAATATCCGGGAAATGTCGGTTCATACCGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCCTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCACCCGGCAAGCTGCCCTGCATGGa                                                                            >  2:605779/1‑149 (MQ=255)

                                                                gcccACCAGATAAAACGAATATCCGGGAAATGTCGGTTCATACCGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCCTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCACCCGGCAAGCTGCCCTGCATGGAag                                                                          <  2:521610/151‑1 (MQ=255)

                                                                  ccaccaGATAAAACGAATATCCGGGAAATGTCGGTTCATACCGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCCTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCACCCGGCAAGCTGCCCTGCATGGAagag                                                                        <  2:643736/151‑1 (MQ=255)

                                                                    accaGATAAAACGAATATCCGGGAAATGTCGGTTCATACCGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCCTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCACCCGGCAAGCTGCCCTGCATGGAagagag                                                                      <  1:571710/151‑1 (MQ=255)

                                                                       aGATACAACGAATATCCGCGAAATGTCGGTTCATACCGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCCTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCACCCGGCAAGCTGCCCTGCATGGAAGAGAGCAc                                                                   <  2:477942/151‑1 (MQ=255)

                                                                       aGATAAAACGAATATCCGGGAAATGTCGGTTCATACCGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCCTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCGCCCGGCAAGCTGCCCTGCATGGAAGAGAGCAc                                                                   <  1:442011/151‑1 (MQ=255)

                                                                         aTAAAACGAATATCCGGGAAATGTCGGTTCATACCGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCCTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCACCCGGCAAGCTGCCCTGCATGGAAGAGAGCACCg                                                                 <  2:94977/151‑1 (MQ=255)

                                                                         aTAAAACGAATATCCGGGAAATGTCGGTTCATACCGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCCTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCACCCGGCAAGCTGCCCTGCATGGAAGAGAGCACCg                                                                 >  1:516647/1‑151 (MQ=255)

                                                                           aaaaCGAATATCCGGGAAATGTCGGTTCATACCGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCCTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCACCCGGCAAGCTGCCCTGCATGGAAGAGAGCACCGc                                                                >  2:18483/1‑150 (MQ=255)

                                                                                gAATATCCGGGAAATGTCGGTTCATACCGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCCTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCACCCGGCAAGCTGCCCTGCATGGAAGAGAGCACCGCAGAAcg                                                          >  1:252644/1‑151 (MQ=255)

                                                                                    atCCGGGAAATGTCGGTTCATACCGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCCTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCACCCGGCAAGCTGCCCTGCATGGAAGAGAGCACCGCAGAAcgccg                                                       <  1:181521/150‑1 (MQ=255)

                                                                                       cGGGAAATGTCGGTTCATACCGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCCTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCACCCGGCAAGCTGCCCTGCATGGAAGAGAGCACCGCAGAACGCCGCGTg                                                   >  2:407178/1‑151 (MQ=255)

                                                                                           aaaTGTCGGTTCATACCGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCCTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCACCCGGCAAGCTGCCCTGCATGGAAGAGAGCACCGCAGAACGCCGCGTGGGGt                                               <  2:237335/151‑1 (MQ=255)

                                                                                              tGTCGGTTCATACCGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCCTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCACCCGGCAAGCTGCCCTGCATGGAAGAGAGCACCGCAGAACGCCGCGTGGGGTTac                                            >  2:107529/1‑151 (MQ=255)

                                                                                                tCGGTTCATACCGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCCTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCACCCGGCAAGCTGCCCTGCATGGAAGAGAGCACCGCAGAACGCCGCGTGGGGTTacc                                           >  1:189163/1‑150 (MQ=255)

                                                                                                   gTTCATACCGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCCTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCACCCGGCAAGCTGCCCTGCATGGAAGAGAGCACCGCAGAACGCCGCGTGGGGTTAccacca                                       <  1:276546/151‑1 (MQ=255)

                                                                                                                 gtTGATATGCGCCACCAGGGTTTTCCAGTGCCTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCACCCGGCAAGCTGCCCTGCATGGAAGAGAGCACCGCAGAACGCCGCGTGGGGTTACCACCATTGGCAAAATGGt                          <  1:593131/150‑1 (MQ=255)

                                                                                                                  ttGATATGCGCCACCAGGGTTTTCCAGTGCCTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCACCCGGCAAGCTGCCCTGCATGGAAGAGAGCTCCGCAGAACGCCGCGTGGGGTTACCACCATTGGCAAAATgtt                          <  2:554546/149‑3 (MQ=255)

                                                                                                                   tGATATGCGCCACCAGGGTTTTCCAGTGCCTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCACCCGGCAAGCTGCCCTGCATGGAAGAGAGCACCGCAGAACGCCGCGTGGGGTTACCACCATTGGCAAAATGGTaa                        <  2:637096/150‑1 (MQ=255)

                                                                                                                      tatGCGCCACCAGGGTTTTCCAGTGCCTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCACCCGGCAAGCTGCCCTGCATGGAAGAGAGCACCGCAGAACGCCGCGTGGGGTTACCACCATTGGCAAAATGGTaagaa                     >  1:46142/1‑150 (MQ=255)

                                                                                                                             caccaGGGTTTTCCAGTGCCTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCACCCGGCAAGCTGCCCTGCATGGAAGAGAGCACCGCAGAACGCCGCGTGGGGTTACCACCATTGGCAAAATGGTAAGAAAGACCaa              >  1:253587/1‑150 (MQ=255)

                                                                                                                                caGGGTTTTCCAGTGCCTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCACCCGGCAAGCTGCCCTGCATGGAAGAGAGCACCGCAGAACGCCGCGTGGGGTTACCACCATTGGCAAAATGGTAAGAAAGACCAAAAcc          >  1:494069/1‑151 (MQ=255)

                                                                                                                                 aGGGTTTTCCAGTGCCTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCACCCGGCAAGCTGCCCTGCATGGAAGAGAGCACCGCAGAACGCCGCGTGGGGTTACCACCATTGGCAAAATGGTAAGAAAGACCAAAAccg         >  2:389335/1‑151 (MQ=255)

                                                                                                                                 aGGGTTTTCCAGTGCCTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCACCCGGCAAGCTGCCCTGCATGGAAGAGAGCACCGCAGAACGCCGCGTGGGGTTACCACCATTGGCAAAATGGTAAGAAAGACCAAAAccg         <  2:650944/151‑1 (MQ=255)

                                                                                                                                        tCCAGTGCCTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCACCCGGCAAGCTGCCCTGCATGGAAGAGAGCACCGCAGAACGCCGCGTGGGGTTACCACCATTGGCAAAATGGTAAGAAAGACCAAAACCGCCGCCgg  >  2:577574/1‑151 (MQ=255)

CGGTTTTTGCCAGGCACGGATCAGGCGATTGGTATCCTGATGATGAGTAAAGTTGGCACCGCCCGCCCACCAGATAAAACGAATATCCGGGAAATGTCGGTTCATACCGTTGTGTTGATATGCGCCACCAGGGTTTTCCAGTGCTTCAACAATGCGGGCAACAGGGATTTTATCCACCGCATCGCAGCCACCCGGCAAGCTGCCCTGCATGGAAGAGAGCACCGCAGAACGCCGCGTGGGGTTACCACCATTGGCAAAATGGTAAGAAAGACCAAAACCGCCGCCGG  >  NC_000913/3715051‑3715337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A