Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,815,521	A→G	V83A (GTG→GCG)	pyrE ←	orotate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,815,521	0	A	G	100.0%	163.4 / NA	52	V83A (GTG→GCG)	pyrE	orotate phosphoribosyltransferase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (23/29); total (23/29)

GCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGC  >  NC_000913/3815374‑3815660

gCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCGCAg                                                                                                                                          <  2:208320/151‑1 (MQ=255)

   gTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCGCAGCgg                                                                                                                                       <  2:566974/151‑1 (MQ=255)

   gTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCCCCCGTGCCGCAGCgg                                                                                                                                       >  1:678173/1‑151 (MQ=255)

   gTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGGCCCGGGCGGGATGCCCCGCCCGTGCCGCCGCgg                                                                                                                                       >  2:124986/1‑151 (MQ=255)

      ccGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCGCAGCGGtt                                                                                                                                     <  1:490613/150‑1 (MQ=255)

         gcggTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCGCAGCGGTTgtgg                                                                                                                                 <  2:432396/151‑1 (MQ=255)

           ggTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCGCAGCGGTTgtggtg                                                                                                                               >  1:593291/1‑151 (MQ=255)

                 cacaTCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCGCAGCGGTTGTGGTGGCAATc                                                                                                                         <  1:259595/151‑1 (MQ=255)

                      catcTACCAGCATTACGCGTCCTTGTAGCGCGCTACCAGCCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCGCAGCGGTTGTGGTGGCAATCGGGa                                                                                                                     <  1:127228/150‑1 (MQ=255)

                           aCCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCGCAGCGGTTGTGGTGGCAATCGGGATc                                                                                                                   <  2:240981/147‑1 (MQ=255)

                           aCCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCGCAGCGGTTGTGGTGGCAATCGGGATCCCt                                                                                                                <  2:423378/150‑1 (MQ=255)

                            ccAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCGCAGCGGTTGTGGTGGCAATCGGGATCCCttt                                                                                                              <  2:342239/151‑1 (MQ=255)

                                      gcgTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCGCAGCGGTTGTGGGGGCCATCGGGATCCCTTTGTAAGCAggg                                                                                                    >  1:435187/1‑151 (MQ=255)

                                         tCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCGCAGCGGTTGTGGTGGCAATCGGGGTCCCTTTGTAAGCAGGGcc                                                                                                  >  1:378977/1‑150 (MQ=255)

                                             tGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCGCAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCaaa                                                                                               <  1:357160/149‑1 (MQ=255)

                                              gTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCCCCAGTGCCGCAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCaaa                                                                                               >  2:633392/1‑148 (MQ=255)

                                              gTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGGCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGGCCAGGGCGTGAGGGTACGCCCCTGCCGCAGCGGGTGGGGTGGCCATCGGGATCCCTTTGTAAGCCGGGCCaa                                                                                                >  2:460164/1‑147 (MQ=255)

                                                    cgcTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGGCGTGATGCTCCGCCAGTGCCGCAGCGGTTGGGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAAcagc                                                                                           >  1:121237/1‑146 (MQ=255)

                                                            cccaGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCTGGTCGTGATGCTCCGCAATTGCCGCAGCGGTTGGGGTGGCAATCGGGATCCCTTTGTAAACAGGGCCCAACAGCAGATCGAAc                                                                                  >  2:526662/2‑147 (MQ=255)

                                                             ccaGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCGCAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCaa                                                                              >  1:229579/1‑150 (MQ=255)

                                                              caGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCGCAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATg                                                                            <  1:8194/151‑1 (MQ=255)

                                                                gATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCGCAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGcc                                                                          >  1:9516/1‑151 (MQ=255)

                                                                    gccgccTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGTAGGTCCAGGTCGTGATGCTCCGCCAGTGCCGCAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGaa                                                                      <  1:372130/151‑1 (MQ=255)

                                                                    gccgccTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCGCAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGaa                                                                      <  2:284062/151‑1 (MQ=255)

                                                                       gccTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGGCGTGATGCTCCGCCAGTGCCGCAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATcc                                                                   >  2:367510/1‑151 (MQ=255)

                                                                                       tttGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCGCAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAg                                                      <  1:9396/148‑1 (MQ=255)

                                                                                       tttGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCGCAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCg                                                    >  1:592482/1‑150 (MQ=255)

                                                                                            ttcttTGCGGTTAAAGCAGTACGGCAGGTCCAGGGCGTGATGCTCCTCCCGTGCCGCAGCGGTTGTGGTGGCCATCGGGGTCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAAACCAACAACGCTTCAGCGGaaaa                                               >  2:213355/1‑150 (MQ=255)

                                                                                                ttGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCGCAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACgg                                           <  1:321172/150‑1 (MQ=255)

                                                                                                    ggTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCGCAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCCCCAACGCTTCAGCGTAAAAACGGCCTa                                       >  2:377356/1‑150 (MQ=255)

                                                                                                     gTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCGCAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAAc                                     <  1:441161/151‑1 (MQ=255)

                                                                                                     gTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCGCAGCGGTTGTGGGGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAAATCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTaa                                      >  2:261758/1‑150 (MQ=255)

                                                                                                        aaaGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCGCAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAg                                   >  1:191313/1‑150 (MQ=255)

                                                                                                        aaaGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCGCAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGt                                  <  2:671001/151‑1 (MQ=255)

                                                                                                        aaaGCAGTACGGCAGGTCCAGGGCGTGATGCTCCGCCAGTGCCGCAGCGGTTGTGGGGGGAAACGGGATCCCTTTGTAAGAAGGGCCAAAAAGCAGAACGAACTCAATGCCGGAAACCACCAACGCTTCAGCGGAAAAAAGGCCTAACa                                    >  2:349106/1‑149 (MQ=255)

                                                                                                         aaGCAGTACGGCAGGTCCCGGTCGCGATGCCCCCCCAGTGCCGCACCGGTTGTGGGGGCCATCGGGATCCCTTTGTAAGCAGGGCCCAACAGCCGATCGCACTCAATGCCGGAATCCACCCACGCCTCCGCGTAAAAACGGccccac                                     >  2:364976/1‑143 (MQ=255)

                                                                                                                aCGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCGCAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGa                            <  2:627319/149‑1 (MQ=255)

                                                                                                                aCGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCGCAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGAt                           <  2:371961/150‑1 (MQ=255)

                                                                                                                aCGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCGCAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGAt                           <  1:513587/150‑1 (MQ=255)

                                                                                                                 cGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCGCAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATcg                         <  2:642557/151‑1 (MQ=255)

                                                                                                                   gCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCGCAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATcgcg                       <  2:457006/151‑1 (MQ=255)

                                                                                                                     aggtccaggtcGTGATGCTCCGCCAGTGCCGCAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGcc                     >  1:637260/1‑151 (MQ=255)

                                                                                                                       gtccaggtcGTGATGCTCCGCCAGTGCCGCAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCg                   <  1:556036/151‑1 (MQ=255)

                                                                                                                         ccaggtcGTGATGCTCCGCCAGTGCCGCAGCGGTTGTGGTGGCAATCGGGATTCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGt                 >  1:366910/1‑151 (MQ=255)

                                                                                                                         ccaggtcGTGATGCTCCGCCAGTGCCGCAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGt                 >  1:182104/1‑151 (MQ=255)

                                                                                                                          caggtcGTGATGCTCCGCCAGTGCCGCAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTa                <  2:623899/151‑1 (MQ=255)

                                                                                                                            ggtcGTGATGCTCCGCCAGTGCCGCAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTAt               <  2:291699/150‑1 (MQ=255)

                                                                                                                             gtcGTGATGCTCCGCCAGTGCCGCAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTa             <  1:119047/151‑1 (MQ=255)

                                                                                                                              tcGTGATGCTCCGCCAGTGCCGCAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTa             >  2:487759/1‑150 (MQ=255)

                                                                                                                               cGTGATGCTCCGCCAGTGCCGCAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTaaa           <  1:100751/151‑1 (MQ=255)

                                                                                                                                        ccGCCAGTGCCGCAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCgg   <  2:657198/150‑1 (MQ=255)

                                                                                                                                        ccGCCAGTGCCGCAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGc  <  1:669204/151‑1 (MQ=255)

GCCGTTCCGGCGGTGATCACATCATCTACCAGCATTACGCGTCCTTGTAACGCGCTACCAACCAGATTGCCGCCTTCACCGTGGTCTTTTGCTTCTTTGCGGTTAAAGCAGTACGGCAGGTCCAGGTCGTGATGCTCCGCCAGTGCCACAGCGGTTGTGGTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGC  >  NC_000913/3815374‑3815660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A