Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,859,124	A→G	G331G (GGT→GGC)	yidK ←	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,859,124	0	A	G	100.0%	151.7 / NA	42	G331G (GGT→GGC)	yidK	putative transporter
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (28/14); total (28/14)

TTTGCGCCCGACGGTGACCAGCTGCTGCGGCTCGGCATTCTGGTTAATGATGCGACGGTAAATACCCATACTGAATAACGTACTGGCGCTATTCAGAAAGCCGTTGAAGGTACTGATCACCGCACCAAATAACACCGCGCCGAAGAAACCCACCATTGGCACTGGCAGAACGTTATTGACCAGCGTCGGGTAGGCCATGTCGGCTTTCGGTAAATCCTGATACAGATGAAACGCAATCAACCCTGGCAGCACCAGTACCAGCGGGTCGAGCATTTTCAGCACCGCCGTTAAC  >  NC_000913/3858977‑3859268

tttGCGCCCGACGGTGACCAGCTGCTGCGGCTCGGCATTCTGGTTAATGATGCGACGGTAAATACCCATACTGAATAACGTACTGGCGCTATTCAGAAAGCCGTTGAAGGTACTGATCACCGCACCAAATAACACCGCGCCGAAGAAGccc                                                                                                                                               >  1:599608/1‑151 (MQ=255)

 ctgagcCCGACGGTGACCAGCTGCTGCGGCTCGGCATTCTGGTTAATGATGCGCCGGTAAATACCCATACTGAATAACGTACTGGCGCTATTCAGAAAGCCGTTGAAGGTACTGATCACCGCACCAAATAACACCCCGCCGCAGAAGccc                                                                                                                                               >  2:81598/5‑150 (MQ=255)

   gcgcCCGACGGTGACCAGCTGCTGCGGCTCGGCATTCTGGTTAATGATGCGACGGTAAATACCCATACTGAATAACGTACTGGCGCTATTCAGAAAGCCGTTGAAGGTACTGATCACCGCACCAAATAACACCGCGCCGAAGAAGCccacc                                                                                                                                            <  1:275090/151‑1 (MQ=255)

      cccGACGGTGACCAGCTGCTGCGGCTCGGCATTCTGGTTAATGATGCGACGGTAAATACCCATACTGAATAACGTACTGGCGCTATTCAGAAAGCCGTTGAAGGTACTGATCACCGCACCAAATAACACCGCGCCGAAGAAGCCCACCAtt                                                                                                                                         <  2:202226/151‑1 (MQ=255)

       ccGACGGTGACCAGCTGCTGCGGCTCGGCATTCTGGTTAATGATGCGACGGTAAATACCCATACTGAATAACGTACTGGCGCTATTCAGAAAGCCGTTGAAGGTACTGATCACCGCACCAAATAACACCGCGCCGAAGAAGCCCACCATTg                                                                                                                                        <  2:242452/151‑1 (MQ=255)

       ccGACGGTGACCAGCTGCTGCGGCTCGGCATTCTGGTTAATGATGCGACGGTAAATACCCATACTGAATAACGTACTGGCGCTATTCAGAAAGCCGTTGAAGGTACTGATCACCGCACCAAATAACACCGCGCCGAAGAAGCCCACCATTg                                                                                                                                        >  1:588142/1‑151 (MQ=255)

                             gCTCGGCATTCTGGTTAATGATGCGACGGTAAATACCCATACTGAATAACGTACTGGCGCTATTCAGAAAGCCGTTGAAGGTACTGATCACCGCACCAAATAACACCGCGCCGAAGAAGCCCACCATTGGCACTGGCAGAACGTTATTGAc                                                                                                                  >  1:450734/1‑151 (MQ=255)

                                  gCATTCTGGTTAATGATGCGACGGTAAATACCCATACTGAATAACGTACTGGCGCTATTCAGAAAGCCGTTGAAGGTACTGATCACCGCACCAAATAACACCGCGCCGAAGAAGCCCACCATTGGCACTGGCAGAACGTTATTGACCAGCg                                                                                                             <  1:648150/151‑1 (MQ=255)

                                    aTTCTGGTTAATGATGCGACGGTAAATACCCATACTGAATAACGTACTGGCGCTATTCAGAAAGCCGTTGAAGGTACTGATCACCGCACCAAATAACACCGCGCCGAAGAAGCCCACCATTGGCACTGGCAGAACGTTATTGACCAGCGTc                                                                                                           >  1:552529/1‑151 (MQ=255)

                                       cTGGTTAATGATGCGACGGTAAATACCCATACTGAATAACGTACTGGCGCTATTCAGAAAGCCGTTGAAGGTACTGATCACCGCACCAAATAACACCGCGCCGAAGAAGCCCACCATTGGCACTGGCAGAACGTTATTGACCAGCGTCggg                                                                                                        >  2:370797/1‑151 (MQ=255)

                                           ttAATGATGCGACGGTAAATACCCATACTGAATAACGTACTGGCGCTATTCAGAAAGCCGTTGAAGGTACTGATCACCGCACCAAATAACACCGCGCCGAAGAAGCCCACCATTGGCACTGGCAGAACGTTATTGACCAGCGTCGGGTAgg                                                                                                    >  2:404796/1‑151 (MQ=255)

                                                 atgCGACGGTAAATACCCATACTGAATAACGTACTGGCGCTATTCAGAAAGCCGTTGAAGGTACTGATCACCGCACCAAATAACACCGCGCCGAAGAAGCCCACCATTGGCACTGGCAGAACGTTATTGACCAGCGTCGGGTAGGCCATGt                                                                                              <  2:551436/151‑1 (MQ=255)

                                                    cGACGGTAAATACCCATACTGAATAACGTACTGGCGCTATTCAGAAAGCCGTTGAAGGTACTGATCACCGCACCAAATAACACCGCGCCGAAGAAGCCCACCATTGGCACTGGCAGAACGTTATTGACCAGCGTCGGGTAGGCCATGTCgg                                                                                           >  2:632598/1‑151 (MQ=255)

                                                       cGGTAAATACCCATACTGAATAACGTACTGGCGCTATTCAGAAAGCCGTTGAAGGTACTGATCACCGCACCAAATAACACCGCGCCGAAGAAGCCCACCATTGGCACTGGCAGAACGTTATTGACCAGCGTCGGGTAGGCCATGTCGGCtt                                                                                        >  2:667291/1‑151 (MQ=255)

                                                           aaaTACCCATACTGAATAACGTACTGGCGCTATTCAGAAAGCCGTTGAAGGTACTGATCACCGCACCAAATAACACCGCGCCGAAGAAGCCCACCATTGGCACTGGCAGAACGTTATTGACCAGCGTCGGGTAGGCCATGTCGGCTTTCgg                                                                                    >  2:384771/1‑151 (MQ=255)

                                                               aCCCATACTGAATAACGTACTGGCGCTATTCAGAAAGCCGTTGAAGGTACTGATCACCGCACCAAATAACACCGCGCCGAAGAAGCCCACCATTGGCACTGGCAGAACGTTATTGACCAGCGTCGGGTAGGCCATGTCGGCTTTCGGTaa                                                                                 >  2:286057/1‑150 (MQ=255)

                                                               aCCCATACTGAATAACGTACTGGCGCTATTCAGAAAGCCGTTGAAGGTACTGATCACCGCACCAAATAACACCGCGCCGAAGAAGCCCACCATTGGCACTGGCAGAACGTTATTGACCAGCGTCGGGTAGGCCATGTCGGCTTTCGGTaa                                                                                 <  1:203062/150‑1 (MQ=255)

                                                                         taTAACGTACTGGCGCTATTCAGAAAGCCGTTGAAGGTACTGATCACCGCACCAAATAACACCGCGCCGAAGAAGCCAACCATTGGCACTGGCAGAACGTTATTGACCAGCGTCGGGTAGGCAATGTCGGCTTTCGGTAAATACTGATAc                                                                       >  2:655506/2‑150 (MQ=255)

                                                                              cGTACTGGCGCTATTCAGAAAGCCGTTGAAGGTACTGATCACCGCACCAAATAACACCGCGCCGAAGAAGCCCACCATTGGCACTGGCAGAACGTTATTGACCAGCGTCGGGTAGGCCATGTCGGCTTTCGGTAAATCCTGATACAGATGa                                                                 <  1:234242/151‑1 (MQ=255)

                                                                                            tCAGAAAGCCGTTGAAGGTACTGATCACCGCACCAAATAACACCGCGCCGAAGAAGCCCACCATTGGCACTGGCAGAACGTTATTGACCAGCGTCGGGTAGGCCATGTCGGCTTTCGGTAAATCCTGATACAGATGAAACGCAATCAAccc                                                   >  2:51425/1‑151 (MQ=255)

                                                                                             cAGAAAGCCGTTGAAGGTACTGATCACCGCACCAAATAACACCGCGCCGAAGAAGCCCACCATTGGCACTGGCAGAACGTTATTGACCAGCGTCGGGTAGGCCATGTCGGCTTTCGGTAAATCCTGATACAGATGAAACGCAATCAACCCt                                                  >  2:443113/1‑151 (MQ=255)

                                                                                               gAAAGCCGTTGAAGGTACTGATCACCGCACCAAATAACGCCGCGCCGAAGAAGCCCACCATTGGCACTGGCAGAACGTTATTGACCAGCGTCGGGTAGGCCATGTCGGCTTTCGGTAAAGCCTGATACAGATGAAACGCAATCAACCCTgg                                                <  2:225779/151‑1 (MQ=255)

                                                                                                  aGCCGTTGAAGGTACTGATCACCGCACCAAATAACACCGCGCCGAAGAAGCCCACCATTGGCACTGGCAGAACGTTATTGACCAGCGTCGGGTAGGCCATGTCGGCTTTCGGTAAATCCTGATACAGATGAAACGCAATCAACCCTGgcag                                             <  2:397546/151‑1 (MQ=255)

                                                                                                   gCCGTTGAAGGTACTGATCACCGCACCAAATAACACCGCGCCGAAGAAGCCCACCATTGGCACTGGCAGAACGTTATTGACCAGCGTCGGGTAGGCCATGTCGGCTTTCGGTAAATCCTGATACAGATGAAACGCAATCAACCCTGgcagc                                            <  1:197988/151‑1 (MQ=255)

                                                                                                    ccGTTGAAGGTACTGATCACCGCACCAAATAACACCGCGCCGAAGAAGCCCACCATTGGCACTGGCAGAACGTTATTGACCAGCGTCGGGTAGGCCATGTCGGCTTTCGGTAAATCCTGATACAGATGAAACGCAATCAACCCTGgcagc                                            >  1:286188/1‑150 (MQ=255)

                                                                                                     cGTTGAAGGTACTGATCACCGCACCAAATAACACCGCGCCGAAGAAGCCCACCATTGGCACTGGCAGAACGTTATTGACCAGCGTCGGGTAGGCCATGTCGGCTTTCGGTAAATCCTGATACAGATGAAACGCAATCAACCCTGGCAGCAc                                          <  2:462396/151‑1 (MQ=255)

                                                                                                       ttGAAGGTACTGATCACCGCACCAAATAACACCGCGCCGAAGAAGCCCACCATTGGCACTGGCAGAACGTTATTGACCAGCGTCGGGTAGGCCATGTCGGCTTTCGGTAAATCCTGATACAGATGAAACGCAATCAACCCTGGCAGCAcc                                         >  2:457900/1‑150 (MQ=255)

                                                                                                         gAAGGTACTGATCACCGCACCAAATAACACCGCGCCGAAGAAGCCCACCATTGGCACTGGCAGAACGTTATTGACCAGCGTCGGGTAGGCCATGTCGGCTTTCGGTAAATCCTGATACAGATGAAACGCAATCAACCCTGGCAGCACCAGt                                      >  1:344441/1‑151 (MQ=255)

                                                                                                             gTACTGATCACCGCACCAAATAACACCGCGCCGAAGAAGCCCACCATTGGCACTGGCAGAACGTTATTGACCAGCGTCGGGTAGGCCATGTCGGCTTTCGGTAAATCCTGATACAGATGAAACGCAATCAACCCTGGCAGCACCAGTAcc                                   >  1:464617/1‑150 (MQ=255)

                                                                                                               aCTGATCACCGCACCAAATAACACCGCGCCGAAGAAGCCCACCATTGGCACTGGCAGAACGTTATTGACCAGCGTCGGGTAGGCCATGTCGGCTTTCGGTAAATCCTGATACAGATGAAACGCAATCAACCCTGGCAGCACCAGTACCAGc                                >  1:140500/1‑151 (MQ=255)

                                                                                                                cTGATCACCGCACCAAATACCTCCGCGCCGAAGAAGCCCACCATTGGCACTGGCAGAACGTTATTGACCAGCGTCGGGTAGGCCATGTCGGCTTTCTTTAAATCCTTATACAGATGAAACGCAATCAACCCTGGCAGCACCAGTACCAg                                 <  1:41682/149‑1 (MQ=255)

                                                                                                                  gATCACCGCACCAAATAACACCGCGCCGAAGAAGCCCACCATTGGCACTGGCAGAACGTTATTGACCAGCGTCGGGGAGGCCATGTCGGCTTTCGGTAAATCCTGATACAGATGAAACGCAATCAACCCTGGCAGCACCAGTACCAGCggg                             >  2:340445/1‑151 (MQ=255)

                                                                                                                      aCCGCACCAAATAACACCGCGCCGAAGAAGCCCACCATTGGCACTGGCAGAACGTTATTGACCAGCGTCGGGTAGGCCATGTCGGCTTTCGGTAAATCCTGATACAGATGAAACGCAATCAACCCTGGCAGCACCAGTACCAGCGGGTCg                          >  1:637746/1‑150 (MQ=255)

                                                                                                                       ccGCACCAAATAACGCCGCGCCGAAGAAGCCCACCATTGGCACTGGCAGAACGTTATTGACCAGCGTCGGGTAGGCCATGTCGGCTTTCGGTAAATCCTGATACAGATGAAACGCAATCAACCCTGGCAGCACCAGTACCAGCGGGTCg                          >  1:232620/1‑149 (MQ=255)

                                                                                                                       ccGCACCAAATAACACCGCGCCGAAGAAGCCCACCATTGGCACTGGCAGAACGTTATTGACCAGCGTCGGGTAGGCCATGTCGGCTTTCGGTAAATCCTGATACAGATGAAACGCAATCAACCCTGGCAGCACCAGTACCAGCGGGTCg                          >  1:148091/1‑149 (MQ=255)

                                                                                                                        cGCACCAAATAACACCGCGCCGAAGAAGCCCACCATTGGCACTGGCAGAACGTTATTGACCAGCGTCGGGTAGGCCATGTCGGCTTTCGGTAAATCCTGATACAGATGAAACGCAATCAACCCTGGCAGCACCAGTACCAGCGGGTCGAGc                       >  1:301774/1‑151 (MQ=255)

                                                                                                                         gCACCAAATAACACCGCGCCGAAGAAGCCCACCATTGGCACTGGCAGAACGTTATTGACCAGCGTCGGGTAGGCCATGTCGGCTTTCGGTAAATCCTGATACAGATGAAACGCAATCAACCCTGGCAGCACCAGTACCAGCGGGTCGAGCa                      <  2:95473/151‑1 (MQ=255)

                                                                                                                                   acacCGCGCCGAAGAAGCCCACCATTGGCACTGGCAGAACGTTATTGACCAGCGTCGGGTAGGCCATGTCGGCTTTCGGTAAATCCTGATACAGATGAAACGCAATCAACCCTGGCAGCACCAGTACCAGCGGGTCGAGCATTTTCAGCAc            <  1:174534/151‑1 (MQ=255)

                                                                                                                                        gcgcCGAAGAAGCCCACCATTGGCACTGGCAGAACGTTATTGACCAGCGTCGGGTAGGCCATGTCGGCTTTCGGTAAATCCTGATACAGATGAAACGCAATCAACCCTGGCAGCACCAGTACCAGCGGGTCGAGCATTTTCAGCAccgccg       >  2:366244/1‑151 (MQ=255)

                                                                                                                                         cgcCGAAGAAGCCCACCATTGGCACTGGCAGAACGTTATTGACCAGCGTCGGGTAGGCCATGTCGGCTTTCGGTAAATCCTGATACAGATGAAACGCAATCAACCCTGGCAGCACCAGTACCAGCGGGTCGAGCATTTTCAGCACCGCCGt      >  1:443815/1‑151 (MQ=255)

                                                                                                                                             gaagaaGCCCACCATTGGCACTGGCAGAACGTTATTGACCAGCGTCGGGTAGGCCATGTCGGCTTTCGGTAAATCCTGATACAGATGAAACGCAATCAACCCTGGCAGCACCAGTACCAGCGGGTCGAGCATTTTCAGCACCGCCGTTAAc  >  2:249898/1‑151 (MQ=255)

                                                                                                                                             gaagaaGCCCACCATTGGCACTGGCAGAACGTTATTGACCAGCGTCGGGTAGGCCATGTCGGCTTTCGGTAAATCCTGATACAGATGAAACGCAATCAACCCTGGCAGCACCAGTACCAGCGGGTCGAGCATTTTCAGCACCGCCGTTAAc  >  1:595921/1‑151 (MQ=255)

                                                                                                                                              aagaagCCCACCATTGGCACTGGCAGAACGTTATTGACCAGCGTCGGGTAGGCCATGTCGGCTTTCGGTAAATCCTGATACAGATGAAACGCAATCAACCCTGGCAGCACCAGTACCAGCGGGTCGAGCATTTTCAGCACCGCCGTTAAc  >  1:264796/1‑150 (MQ=255)

TTTGCGCCCGACGGTGACCAGCTGCTGCGGCTCGGCATTCTGGTTAATGATGCGACGGTAAATACCCATACTGAATAACGTACTGGCGCTATTCAGAAAGCCGTTGAAGGTACTGATCACCGCACCAAATAACACCGCGCCGAAGAAACCCACCATTGGCACTGGCAGAACGTTATTGACCAGCGTCGGGTAGGCCATGTCGGCTTTCGGTAAATCCTGATACAGATGAAACGCAATCAACCCTGGCAGCACCAGTACCAGCGGGTCGAGCATTTTCAGCACCGCCGTTAAC  >  NC_000913/3858977‑3859268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A