Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,889,885	T→C	Y386H (TAT→CAT)	tnaA →	tryptophanase/L‑cysteine desulfhydrase, PLP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,889,885	0	T	C	100.0%	166.9 / NA	44	Y386H (TAT→CAT)	tnaA	tryptophanase/L‑cysteine desulfhydrase, PLP‑dependent
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (19/25); total (19/25)

ACAGTATCTGGTCGATGGTCTGGAAGAGATTGGCGTTGTCTGCCAGCAGGCGGGCGGTCACGCGGCATTCGTTGATGCCGGTAAACTGTTGCCGCATATCCCGGCAGACCAGTTCCCGGCACAGGCGCTGGCCTGCGAGCTGTATAAAGTCGCCGGTATCCGTGCGGTAGAAATTGGCTCTTTCCTGTTAGGCCGCGATCCGAAAACCGGTAAACAACTGCCATGCCCGGCTGAACTGCTGCGTTTAACCATTCCGCGCGCAACATATACTCAAACACATATGGACTTC  >  NC_000913/3889743‑3890031

aCAGTATCTGGTCGATGGTCTGGAAGAGATTGGCGTTGTCTGCCAGCAGGCGGGCGGTCACGCGGCATTCGTTGATGCCGGTAAACTGTTGCCGCATATCCCGGCAGACCAGTTCCCGGCACAGGCGCTGGCCTGCGAGCTGCATAAAGt                                                                                                                                             <  2:278396/150‑1 (MQ=255)

aCAGTATCTGGTCGATGGTCTGGAAGAGATTGGCGTTGTCTGCCAGCAGGCGGGCGGTCACGCGGCATTCGTTGATGCCGGTAAACTGTTGCCGCATATCCCGGCAGACCAGTTCCCGGCACAGGCGCTGGCCTGCGAGCTGCATAAAGt                                                                                                                                             <  1:507992/150‑1 (MQ=255)

aCAGTATCTGGTCGATGGTCTGGAAGAGATTGGCGTTGTCTGCCAGCAGGCGGGCGGTCACGCGGCATTCGTTGATGCCGGTAAACTGTTGCCGCATATCCCGGCAGACCAGTTCCCGGCACAGGCGCTGGCCTGCGAGCTGCATAAAGTc                                                                                                                                            <  2:254713/151‑1 (MQ=255)

    tATCTGGTCGATGGTCTGGAAGAGATTGGCGTTGTCTGCCAGCAGGCGGGCGGTCACGCGGCATTCGTTGATGCCGGTAAACTGTTGCCGCATATCCCGGCAGACCAGTTCCCGGCACAGGCGCTGGCCTGCGAGCTGCATAAAGTCGCCg                                                                                                                                        >  2:629134/1‑151 (MQ=255)

     aTCTGGTCGATGGTCTGGAAGAGATTGGCGTTGTCTGCCAGCAGGCGGGCGGTCACGCGGCATTCGTTGATGCCGGTAAACTGTTGCCGCATATCCCGGCAGACCAGTTCCCGGCACAGGCGCTGGCCTGCGAGCTGCATAAAGTCGCCg                                                                                                                                        <  1:475416/150‑1 (MQ=255)

       cTGGTCGATGGTCTGGAAGAGATTGGCGTTGTCTGCCAGCAGGCGGGCGGTCACGCGGCATTCGTTGATGCCGGTAAACTGTTGCCGCATATCCCGGCAGACCAGTTCCCGGCACAGGCGCTGGCCTGCGAGCTGCATAAAGTCGCCGGt                                                                                                                                      >  1:528970/1‑150 (MQ=255)

            cGATGGTCTGGAAGAGATTGGCGTTGTCTGCCAGCAGGCGGGCGGTCACGCGGCATTCGTTGATGCCGGTAAACTGTTGCCGCATATCCCGGCAGACCAGTTCCCGGCACAGGCGCTGGCCTGCGAGCTGCATAAAGTCGCCGGTATCCGt                                                                                                                                >  2:473400/1‑151 (MQ=255)

            cGATGGTCTGGAAGAGATTGGCGTTGTCTGCCAGCAGGCGGGCGGTCACGCGGCATTCGTTGATGCCGGTAAACTGTTGCCGCATATCCCGGCAGACCAGTTCCCGGCACAGGCGCTGGCCTGCGAGCTGCATAAAGTCGCCGGTATCCGt                                                                                                                                >  1:498028/1‑151 (MQ=255)

              aTGGTCTGGAAGAGATTGGCGTTGTCTGCCAGCAGGCGGGCGGTCACGCGGCATTCGTTGATGCCGGTAAACTGTTGCCGCATATCCCGGCAGACCAGTTCCCGGCACAGGCGCTGGCCTGCGAGCTGCATAAAGTCGCCGGTATCCGTGc                                                                                                                              >  2:99396/1‑151 (MQ=255)

                     ggAAGAGATTGGCGTTGTCTGCCAGCAGGCGGGCGGTCACGCGGCATTCGTTGATGCCGGTAAACTGTTGCCGCATATCCCGGCAGACCAGTTCCCGGCACAGGCGCTGGCCTGCGAGCTGCATAAAGTCGCCGGTATCCGTGCGGTAGa                                                                                                                        >  2:264570/1‑150 (MQ=255)

                      gAAGAGATTGGCGTTGTCTGCCAGCAGGCGGGCGGTCACGCGGCATTCGTTGATGCCGGTAAACTGTTGCCGCATATCCCGGCAGACCAGTTCCCGGCACAGGCGCTGGCCTGCGAGCTGCATAAAGTCGCCGGTATCCGTGCGGTAGaaa                                                                                                                      <  1:272167/151‑1 (MQ=255)

                       aaGAGATTGGCGTCGTCTGCCAGCAGGCGGGCGGTCACGCGGCATTCGTTGATGCCGGTAAACTGTTGCCGCATATCCCGGCAGACCAGTTCCCGGCACAGGCGCTGGCCTGCGAGCTGCATAAAGTCGCCGGTATCCGTGCGGTAGAAAt                                                                                                                     >  1:313919/1‑151 (MQ=255)

                        agagATTGGCGTTGTCTTCCAGCAGGCGGGCGGTCACGCGGCATTCGTTGATGCCGGTAAACTGTTGCCGCATATCCCGGCAGACCAGTTCCCGGCACAGGCGCTGGCCTGCGAGCTGCATAAAGTCGCCGGTATCCGTGCGGTAGAAAtt                                                                                                                    >  1:390974/1‑151 (MQ=255)

                                gCGTTGTCTGCCAGCAGGCGGGCGGTCACGCGGCATTCGTTGATGCCGGTAAACTGTTGCCGCATATCCCGGCAGACCAGTTCCCGGCACAGGCGCTGGCCTGCGAGCTGCATAAAGTCGCCGGTATCCGTGCGGTAGAAATTGGCTCttt                                                                                                            <  1:362843/151‑1 (MQ=255)

                                         gCCAGCAGGCGGGCGGTCACGCGGCATTCGTTGATGCCGGTAAACTGTTGCCGCATATCCCGGCAGACCAGTTCCCGGCACAGGCGCTGGCCTGCGAGCTGCATAAAGTCGCCGGTATCCGTGCGGTAGAAATTGGCTCTTTCCTGTTa                                                                                                     <  2:621471/149‑1 (MQ=255)

                                          ccaccaGGCTGGCGGTCACGCGGCATTCGTTTATGCCTGTAAACTGTTGCCGCATATCCCTCCAGACCAGTTCCCTGCACATTCACTATCCTTCGAGCTGCATAAAGTCGCCCTTATCCGTTCGCTAGAAATTCTCTCTTTCCTTTTAgac                                                                                                  <  2:124776/151‑3 (MQ=255)

                                           cagcagGCGGGCGGTCACGCGGCATTCGTTGATGCCGGTAAACTGTTGCCGCATATCCCGGCAGACCAGTTCCCGGCACAGGCGCTGGCCTGCGAGCTGCATAAAGTCGCCGGTATCCGTGCGGTAGAAATTGGCTCTTTCCTGTTAGGcc                                                                                                 <  2:117108/151‑1 (MQ=255)

                                               agGCGGGCGGTCACGCGGCATTCGTTGATGCCGGTAAACTGTTGCCGCATATCCCGGCAGACCAGTTCCCGGCACAGGCGCTGGCCTGCGAGCTGCATAAAGTCGCCGGTATCCGTGCGGTAGAAATTGGCTCTTTCCTGTTAGGCcgcg                                                                                              >  1:199921/1‑150 (MQ=255)

                                               agGCGGGCGGTCACGCGGCATTCGTTGATGCCGGTAAACTGTTGCCGCATATCCCGGCAGACCAGTTCCCGGCACAGGCGCTGGCCTGCGAGCTGCATAAAGTCGCCGGTATCCGTGCGGTAGAAATTGGCTCTTTCCTGTTAGGCCGCGa                                                                                             <  2:508601/151‑1 (MQ=255)

                                                   gggcggTCACGCGGCATTCGTTGATGCCGGTAAACTGTTGCCGCATATCCCGGCAGACCAGTTCCCGGCACAGGCGCTGGCCTGCGAGCTGCATAAAGTCGCCGGTATCCGTGCGGTAGAAATTGGCTCTTTCCTGTTAGGCCGCGATCCg                                                                                         >  2:90454/1‑151 (MQ=255)

                                                          cACGCGGCATTCGTTGATGCCGGTAAACTGTTGCCGCATATCCCGGCAGACCAGTTCCCGGCACAGGCGCTGGCCTGCGAGCTGCATAAAGTCGCCGGTATCCGTGCGGTAGAAATTGGCTCTTTCCTGTTAGGCCGCGATCCGAAAAcc                                                                                   <  2:281826/150‑1 (MQ=255)

                                                                gCATTCGTTGATGCCGGTAAACTGTTGCCGCGTATCCCGGCAGACCAGTTCCCGGCACAGGCGCTGGCCTGCGAGCTGCATAAAGTCGCCGGTATCCGTGCGGTAGAAATTGGCTCTTTCCTGTTAGGCCGCGATCCGAAAACCGGTAaac                                                                            <  1:571433/151‑1 (MQ=255)

                                                                  aTTCGTTGATGCCGGTAAACTGTTGCCGCATATCCCGGCAGACCAGTTCCCGGCACAGGCGCTGGCCTGCGAGCTGCATAAAGTCGCCGGTATCCGTGCGGTAGAAATTGGCTCTTTCCTGTTAGGCCGCGATCCGAAAACCGGTAaacaa                                                                          <  2:110288/151‑1 (MQ=255)

                                                                  aTTCGTTGATGCCGGTAAACTGTTGCCGCATATCCCGGCAGACCAGTTCCCGGCACAGGCGCTGGCCTGCGAGCTGCATAAAGTCGCCGGTATCCGTGCGGTAGAAATTGGCTCTTTCCTGTTAGGCCGCGATCCGAAAACCGGTAaacaa                                                                          <  1:629153/151‑1 (MQ=255)

                                                                    tCGTTGATGCCGGTAAACTGTTGCCGCATATCCCGGCGGACCAGTTCCCGGCACAGGCGCTGGCCTGCGAGCTGCATAAAGTCGCCGGTATCCGTGCGGTAGAAATTGGCTCTTTCCTGTTAGGCCGCGATCCGAAAACCGGTAaacaac                                                                         <  1:561655/150‑1 (MQ=255)

                                                                         gATGCCGGTAAACTGTTGCCGCATATCCCGGCAGACCAGTTCCCGGCACAGGCGCTGGCCTGCGAGCTGCATAAAGTCGCCGGTATCCGTGCGGTAGAAATTGGCTCTTTCCTGTTAGGCCGCGATCCGAAAACCGGTAAACAACTGCCAt                                                                   >  2:511703/1‑151 (MQ=255)

                                                                             ccGGTAAACTGTTGCCGCATATCCCGGCAGACCAGTTCCCGGCACAGGCGCTGGCCTGCGAGCTGCATAAAGTCGCCGGTATCCGTGCGGTAGAAATTGGCTCTTTCCTGTTAGGCCGCGATCCGAAAACCGGTAAACAACTGCCATGccc                                                               <  2:98808/151‑1 (MQ=255)

                                                                             ccGGTAAACTGTTGCCGCATATCCCGGCAGACCAGTTCCCGGCACAGGCGCTGGCCTGCGAGCTGCATAAAGTCGCCGGTATCCGTGCGGTAGAAATTGGCTCTTTCCTGTTAGGCCGCGATCCGAAAACCGGTAAACAACTGCCATGccc                                                               >  2:275247/1‑151 (MQ=255)

                                                                                 tAAACTGTTGCCGCATATCCCGGCAGACCAGTTCCCGGCACAGGCGCTGGCCTGCGAGCTGCATAAAGTCGCCGGTATCCGTGCGGTAGAAATTGGCTCTTTCCTGTTAGGCCGCGATCCGAAAACCGGTAAACAACTGCCATGCCCGGCt                                                           >  2:226689/1‑151 (MQ=255)

                                                                                            cGCATATCCCGGCAGACCAGTTCCCGGCACAGGCGCTGGCCTGCGAGCTGCATAAAGTCGCCGGTATCCGTGCGGTAGAAATTGGCTCTTTCCTGTTAGGCCGCGGTCCGAAAACCGGTAAACAACTGCCATGCCCGGCTGAACTGCTGCg                                                >  2:462636/1‑151 (MQ=255)

                                                                                             gCATATCCCGGCAGACCAGTTCCCGGCACAGGCGCTGGCCTGCGAGCTGCATAAAGTCGCCGGTATCCGTGCGGTAGAAATTGGCTCTTTCCTGTTAGGCCGCGATCCGAAAACCGGTAAACAACTGCCATGCCCGGCTGAACTGCTGCGt                                               <  2:136724/151‑1 (MQ=255)

                                                                                               atatCCCGGCAGACCAGTTCCCGGCACAGGCGCTGGCCTGCGAGCTGCATAAAGTCGCCGGTATCCGTGCGGTAGAAATTGGCTCTTTCCTGTTAGGCCGCGATCCGAAAACCGGTAAACAACTGCCATGCCCGGCTGAACTGCTGCGttt                                             <  2:261312/151‑1 (MQ=255)

                                                                                                      ggCAGACCAGTTCCCGGCACAGGCGCTGGCCTGCGAGCTGCATAAAGTCGCCGGTATCCGTGCGGTAGAAATTGGCTCTTTCCTGTTAGGCCGCGATCCGAAAACCGGTAAACAACTGCCATGCCCGGCTGAACTGCTGCGTTTAACCAtt                                      <  1:643671/151‑1 (MQ=255)

                                                                                                        cAGACCAGTTCCCGGCACAGGCGCTGGCCTGCGAGCTGCATAAAGTCGCCGGTATCCGTGCGGTAGAAATTGGCTCTTTCCTGTTAGGCCGCGATCCGAAAACCGGTAAACAACTGCCATGCCCGGCTGAACTGCTGCGTTTAACCATTcc                                    <  2:618197/151‑1 (MQ=255)

                                                                                                             cAGTTCCCGGCACAGGCGCTGGCCTGCGAGCTGCATAAAGTCGCCGGTATCCGTGCGGTAGAAATTGGCTCTTTCCTGTTAGGCCGCGATCCGAAAACCGGTAAACAACTGCCATGCCCGGCTGAACTGCTGCGTTTAACCATTCcgcgcg                               >  2:468023/1‑151 (MQ=255)

                                                                                                                   ccGGCACAGGCGCTGGCCTGCGAGCTGCATAAAGTCGCCGGTATCCGTGCGGTAGAAATTGGCTCTTTCCTGTTAGGCCGCGATCCGAAAACCGGTAAACATCTGCCATGCCCGGCTGAACTGCTGCGTTTAACCATTCCGCGCGCAACat                         <  2:159732/151‑1 (MQ=255)

                                                                                                                   ccGGCACAGGCGCTGGCCTGCGAGCTGCATAAAGTCGCCGGTATCCGTGCGGTAGAAATTGGCTCTTTCCTGTTAGGCCGCGATCCGAAAACCGGTAAACAACTGCCATGCCCGGCTGAACTGCTGCGTTTAACCATTCCGCGCGCAACat                         >  1:239244/1‑151 (MQ=255)

                                                                                                                         caGGCGCTGGCCTGCGAGCTGCATAAAGTCGCCGGTATCCGTGCGGTAGAAATTGGCTCTTTCCTGTTAGGCCGCGATCCGAAAACCGGTAAACAACTGCCATGCCCGGCTGAACTGCTGCGTTTAACCATTCCGCGCGCAACATATACt                    <  2:301048/150‑1 (MQ=255)

                                                                                                                         caGGCGCTGGCCTGCGAGCTGCATAAAGTCGCCGGTATCCGTGCGGTAGAAATTGGCTCTTTCCTGTTAGGCCGCGATCCGAAAACCGGTAAACAACTGCCATGCCCGGCTGAACTGCTGCGTTTAACCATTCCGCGCGCAACATATACTc                   <  1:560406/151‑1 (MQ=255)

                                                                                                                           ggCGCTGGCCTGCGAGCTGCATAAAGTCGCCGGTATCCGTGCGGTAGAAATTGGCTCTTTCCTGTTAGGCCGCGATCCGAAAACCGGTAAACAACTGCCATGCCCGGCTGAACTGCTGCGTTTAACCATTCCGCGCGCAACATATACTCa                  >  1:509802/1‑150 (MQ=255)

                                                                                                                             cgcTGGCCTGCGAGCTGCATAAAGTCGCCGGTATCCGTGCGGTAGAAATTGGCTCTTTCCTGTTAGGCCGCGATCCGAAAACCGGTAAACAACTGCCATGCCCGGCTGAACTGCTGCGTTTAACCATTCCGCGCGCAACATATACTCAAac               >  2:562673/1‑151 (MQ=255)

                                                                                                                                 ggCCTGCGAGCTGCATAAAGTCGCCGGTATCCGTGCGGTAGAAATTGGCTCTTTCCTGTTAGGCCGCGATCCGAAAACCGGTAAACAACTGCCATGCCCGGCTGAACTGCTGCGTTTAACCATTCCGCGCGCAACATATACTCAAacac             <  2:179793/149‑1 (MQ=255)

                                                                                                                                   ccTGCGAGCTGCATAAAGTCGCCGGTATCCGTGCGGTAGAAATTGGCTCTTTCCTGTTAGGCCGCGATCCGAAAACCGGTAAACAACTGCCATGCCCGGCTGAACTGCTGCGTTTAACCATTCCGCGCGCAACATATACTCAAACACatat         <  1:110796/151‑1 (MQ=255)

                                                                                                                                          gCTGCATAAAGTCGCCGGTATCCGTGCGGTAGAAATTGGCTCTTTCCTGTTAGGCCGCGATCCGAAAACCGGTAAACAACTGCCATGCCCGGCTGAACTGCTGCGTTTAACCATTCCGCGCGCAACATATACTCAAACACATATGGACTTc  >  1:154958/1‑151 (MQ=255)

ACAGTATCTGGTCGATGGTCTGGAAGAGATTGGCGTTGTCTGCCAGCAGGCGGGCGGTCACGCGGCATTCGTTGATGCCGGTAAACTGTTGCCGCATATCCCGGCAGACCAGTTCCCGGCACAGGCGCTGGCCTGCGAGCTGTATAAAGTCGCCGGTATCCGTGCGGTAGAAATTGGCTCTTTCCTGTTAGGCCGCGATCCGAAAACCGGTAAACAACTGCCATGCCCGGCTGAACTGCTGCGTTTAACCATTCCGCGCGCAACATATACTCAAACACATATGGACTTC  >  NC_000913/3889743‑3890031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A