Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,979,674	T→C	G358G (GGT→GGC)	wzyE →	putative ECA polysaccharide chain elongation protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,979,674	0	T	C	100.0%	159.2 / NA	44	G358G (GGT→GGC)	wzyE	putative ECA polysaccharide chain elongation protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (25/19); total (25/19)

GCCCGAGTATGGTGCTGAACTCAGCCAACTACTTTACCTGGGAAGTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGTCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGCGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTC  >  NC_000913/3979526‑3979820

gCCCGAGTATGGTGCTGAACTCAGCCAACTACTTTACCTGGGAAGTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCt                                                                                                                                                  >  1:89429/1‑151 (MQ=255)

  ccGAGTATGGTGCTGAACTCAGCCAACTACTTTACCTGGGAAGTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGGGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTg                                                                                                                                                 >  2:476116/1‑150 (MQ=255)

   cGAGTATGGTGCTGAACTCAGCCAACTACTTTACCTGGGAAGTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGat                                                                                                                                               >  1:66283/1‑151 (MQ=255)

   cGAGTATGGTGCTGAACTCAGCCAACTACTTTACCTGGGAAGTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGat                                                                                                                                               <  1:61171/151‑1 (MQ=255)

      gTATGGTGCTGAACTCAGCCAACTACTTTACCTGGGAAGTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGatca                                                                                                                                             <  2:672152/150‑1 (MQ=255)

           gTGCTGAACTCAGCCAACTACTTTACCTGGGAAGTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAAt                                                                                                                                       <  1:538575/151‑1 (MQ=255)

                 aaCTCAGCCAACTACTTTACCTGGGAAGTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCg                                                                                                                                 >  2:676593/1‑151 (MQ=255)

                     cAGCCAACTACTTTACCTGGGAAGTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTg                                                                                                                             >  1:102535/1‑151 (MQ=255)

                     cAGCCAACTACTTTACCTGGGAAGTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTg                                                                                                                             <  1:616568/151‑1 (MQ=255)

                             tactTTACCTGGGAAGTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATg                                                                                                                     >  1:338790/1‑151 (MQ=255)

                               ctTTCCCTGGGAAGTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTAGGa                                                                                                                    <  1:170141/150‑1 (MQ=255)

                               ctTTACCTGGGAAGTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATg                                                                                                                     >  1:651732/1‑149 (MQ=255)

                                   aCCTGGGAAGTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTgg                                                                                                               >  2:413864/1‑151 (MQ=255)

                                    ccTGGGAAGTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTggg                                                                                                              <  1:453208/151‑1 (MQ=255)

                                      tGGGCAGTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGc                                                                                                             >  1:604367/1‑150 (MQ=255)

                                          aaGTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACcg                                                                                                        >  2:235424/1‑151 (MQ=255)

                                            gTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACcgcg                                                                                                      >  1:508204/1‑151 (MQ=255)

                                              gCTGAATAACCGCTCCGGGCTGGCGATCTCGCCTACGATTATAGGCTTGCTGGTGGTGATGGGCGGAGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCCTCAAATGGTGCGACTGGCTGTATGAGCTGGGCAGCCGCGa                                                                                                     <  2:604678/150‑1 (MQ=255)

                                              gCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGa                                                                                                     <  2:548464/150‑1 (MQ=255)

                                                        cACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCt                                                                                          >  2:438507/1‑151 (MQ=255)

                                                          cTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCtat                                                                                        >  1:137136/1‑151 (MQ=255)

                                                           tCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCtat                                                                                        >  1:481009/1‑150 (MQ=255)

                                                                aCTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAg                                                                                    >  2:559151/1‑149 (MQ=255)

                                                                    gCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCg                                                                               >  2:350126/1‑150 (MQ=255)

                                                                    gCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGa                                                                              <  1:595646/151‑1 (MQ=255)

                                                                          tcGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGATATTGc                                                                        >  2:257927/1‑151 (MQ=255)

                                                                           cGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGat                                                                             <  2:420798/145‑1 (MQ=255)

                                                                           cGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGATAtt                                                                          <  1:343942/148‑1 (MQ=255)

                                                                           cGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGATATTGca                                                                       <  2:487261/151‑1 (MQ=255)

                                                                            gCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGATATTGca                                                                       <  2:346554/150‑1 (MQ=255)

                                                                                      ataGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGc                                                             <  1:577998/150‑1 (MQ=255)

                                                                                        aGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCttt                                                          >  2:242257/1‑151 (MQ=255)

                                                                                            tCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTgggg                                                      >  2:586327/1‑151 (MQ=255)

                                                                                             cACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTggg                                                       <  2:371786/149‑1 (MQ=255)

                                                                                                 ggtggtGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGCGATc                                                 <  1:668004/151‑1 (MQ=255)

                                                                                                  gtggtgATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGCGATCt                                                >  1:88280/1‑151 (MQ=255)

                                                                                                     gtgATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGCGATCTTCa                                             <  1:54879/151‑1 (MQ=255)

                                                                                                      tgATGGGCGTCGCCTTGTTCATCCCGCTCGTGGCGATCGTGGTTGGCCTGATCATCAAATTGTTCTACTGGCTGTATGAGCTGGGCAACCGCGAGCTTAATCGCTGTAAATCTGCGATATTGCGCAGTTTCTGCTTTGGGGCGATcct                                               <  2:593448/148‑3 (MQ=255)

                                                                                                            gcggcgCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGCGATCTTCAATATGAt                                      <  2:400332/151‑1 (MQ=255)

                                                                                                                 gcgTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGCGATCTTCAATATGATCGTGc                                 >  2:35073/1‑151 (MQ=255)

                                                                                                                    ttgttCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGCGATCTTCAATATGATCGTGCTgg                              >  2:670894/1‑151 (MQ=255)

                                                                                                                       ttCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGCGATCTTCAATATGATCGTGCTGgcgc                           >  2:240349/1‑151 (MQ=255)

                                                                                                                        tCATCCCGCTCGGGGCGATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGCGATCTTCAATATGATCGTGCTGGcgcg                          >  1:675349/1‑151 (MQ=255)

                                                                                                                                        gATCGTGGTTGGCCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGCGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGATTCg          >  1:367372/1‑151 (MQ=255)

                                                                                                                                             tggttggcCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGCGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGATTCGTTTGt     >  1:89725/1‑151 (MQ=255)

                                                                                                                                                 tggcCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGCGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGATTCGTTTGtctc  >  2:534129/1‑150 (MQ=255)

GCCCGAGTATGGTGCTGAACTCAGCCAACTACTTTACCTGGGAAGTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCACTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGTCTGATCATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCTGCGATATTGCACAGTTTCTGCTTTGGGGCGATCTTCAATATGATCGTGCTGGCGCGTGAAGGGCTGGATTCGTTTGTCTC  >  NC_000913/3979526‑3979820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A