Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A7 F50 I2 R1 209 44.2 1360995 98.7% 1343302 150.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,139,721 Δ1 bp coding (2501/2502 nt) frwA ← putative PTS enzyme: Hpr, enzyme I and II components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,139,7180T.100.0% 209.8 / NA 44intergenic (‑10/+2)fsaB/frwAfructose‑6‑phosphate aldolase 2/putative PTS enzyme: Hpr, enzyme I and II components
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (24/20);  total (24/20)

GCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCGGCGTTAACCAGCGCGTTACGGAATTCTTCGTGCATGATGCGGCGAGCGAGGCGCGAGAAAATGCGCATATGCTGATCGCCCGCAGCGTGTTT  >  NC_000913/4139570‑4139863
                                                                                                                                                    |                                                                                                                                                 
gCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCttt                                                                                                                                                 >  1:414970/1‑151 (MQ=255)
 cAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCttta                                                                                                                                                <  1:667527/151‑2 (MQ=255)
 cAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCttt                                                                                                                                                 >  1:464630/1‑150 (MQ=255)
  aGACGCGGCAGCACTTCCCATATGGACTCCTTGTTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGCCGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCtttac                                                                                                                                               >  2:203439/1‑149 (MQ=255)
     cgcgGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCC‑TTTAcagt                                                                                                                                           <  1:620680/151‑1 (MQ=255)
          cagcaCTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCC‑TTTAcagttcc                                                                                                                                        >  1:478437/1‑149 (MQ=255)
           agcaCTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCC‑TTTAcagttccagt                                                                                                                                     >  2:432128/1‑151 (MQ=255)
           agcaCTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCC‑TTTAcagttccagt                                                                                                                                     >  1:28620/1‑151 (MQ=255)
            gcaCTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCC‑TTTAcagttccagtt                                                                                                                                    >  1:654410/1‑151 (MQ=255)
              aCTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCC‑TTTAcagttccagttc                                                                                                                                   >  2:114874/1‑150 (MQ=255)
              aCTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCC‑TTTACAGTTCCAGTTCa                                                                                                                                  <  1:284996/151‑1 (MQ=255)
                 tCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCC‑TTTACAGTTCCAGTTCAt                                                                                                                                 <  1:573695/149‑1 (MQ=255)
                   ccATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCC‑TTTACAGTTCCAGTTCATGTTg                                                                                                                             <  1:612561/151‑1 (MQ=255)
                     atatGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCC‑TTTACAGTTCCAGTTCATGTTgca                                                                                                                           <  2:508166/151‑1 (MQ=255)
                     atatGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCC‑TTTACAGTTCCAGTTCATGTTgc                                                                                                                            >  1:636439/1‑150 (MQ=255)
                     atatGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTATCAAGATACAGTTCCATGATGTTTCC‑TTTACAGTTCCAGTTCATGTTgc                                                                                                                            >  1:550769/1‑150 (MQ=255)
                        tGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCC‑TTTACAGTTCCAGTTCATGTTgcagc                                                                                                                         >  2:458598/1‑150 (MQ=255)
                         ggACCCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCC‑TTTACAGTTCCAGTTCATGTTGcagcag                                                                                                                       <  2:430393/151‑1 (MQ=255)
                             tCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCC‑TTTACAGTTCCAGTTCATGTTGCAGCAGGCTg                                                                                                                   >  1:27780/1‑151 (MQ=255)
                               cTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCC‑TTTACAGTTCCAGTTCATGTTGCAGCAGGCTgg                                                                                                                  >  2:399987/1‑150 (MQ=255)
                                 tGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCTGTGTCCAGATACAGTTCCATGATGTTTCC‑TTTACAGTTCCAGTTCATGTTGCAGCAGTCTGGCg                                                                                                                <  2:51293/150‑1 (MQ=255)
                                          cGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCC‑TTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCgg                                                                                                      <  2:613405/151‑1 (MQ=255)
                                            aTAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCC‑TTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCa                                                                                                    <  2:602921/151‑1 (MQ=255)
                                                        ttAGTTGTAACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCC‑TTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCGGAGtt                                                                                         >  2:655534/1‑150 (MQ=255)
                                                              gTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCC‑TTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCGGCGTTAACCAg                                                                                   <  2:471189/150‑1 (MQ=255)
                                                                   cccGGGAATGGGGAAAATGCGGGCCAGGCCGTTGGCTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCC‑TTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCGGCGTTAACCAGCGCGtt                                                                             <  2:379497/151‑1 (MQ=255)
                                                                   cccGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCC‑TTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCGGCGTTAACCAGCGCGtt                                                                             <  1:162211/151‑1 (MQ=255)
                                                                    ccGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCC‑TTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCGGCGTTAACCAGCGCGtt                                                                             >  1:89356/1‑150 (MQ=255)
                                                                    ccGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCC‑TTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCGGCGTTAACCAGCGCGTTa                                                                            <  1:207445/151‑1 (MQ=255)
                                                                       gCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCC‑TTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCGGCGTTAACCAGCGCGTTACgg                                                                         <  1:512841/151‑1 (MQ=255)
                                                                         aaTGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCC‑TTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCGGCGTTAACCAGCGCGTTACGGa                                                                        >  2:399855/1‑150 (MQ=255)
                                                                          aTGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCC‑TTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCGGCGTTAACCAGCGCGTTACGGAAt                                                                      >  1:322860/1‑151 (MQ=255)
                                                                             gggAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCC‑TTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCGGCGTTAACCAGCGCGTTACGGAAttct                                                                   >  1:520168/1‑151 (MQ=255)
                                                                                         gCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCC‑TTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCGGCGTTAACCAGCGCGTTACGGAATTCTTCGTGCatgatg                                                       <  2:425239/151‑1 (MQ=255)
                                                                                           cAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCC‑TTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCGGCGTTAACCAGCGCGTTACGGAATTCTTCGTGCATGATgcg                                                     >  2:401797/1‑151 (MQ=255)
                                                                                            aGACGTTCGACTTCTGCGACGCTAGCGGTGTCCAGATACAGTTCCATGATGTTTCC‑TTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCGGCGTTAACCAGCGCGTTACGGAATTCTTCGTGCATGATgc                                                      >  2:26899/1‑149 (MQ=255)
                                                                                                        tCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCC‑TTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCGGCGTTAACCAGCGCGTTACGGAATTCTTCGTGCATGATGCGGCGAGCGAGgcgc                                        >  2:618219/1‑151 (MQ=255)
                                                                                                           gCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCC‑TTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCGGCGTTAACCAGCGCGTTACGGAATTCTTCGTGCATGATGCGGCGAGCGAGGCGCga                                      <  2:654389/150‑1 (MQ=255)
                                                                                                             gACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCC‑TTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCGGCGTTAACCAGCGCGTTACGGAATTCTTCGTGCATGATGCGGCGAGCGAGGCGCGAGaa                                   <  1:222297/151‑1 (MQ=255)
                                                                                                                  tcgCGGTGTCCAGATACAGTTCCATGATGTTTCC‑TTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCGGCGTTAACCAGCGCGTTACGGAATTCTTCGTGCATGATGCGGCGAGCGAGGCGCGAGAAAATgc                              >  1:116937/3‑151 (MQ=255)
                                                                                                                     cGGTGTCCAGATACAGTTCCATGATGTTTCC‑TTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCGGCGTTAACCAGCGCGTTACGGAATTCTTCGTGCATGATGCGGCGAGCGAGGCGCGAGAAAATgcgc                            >  2:445636/1‑150 (MQ=255)
                                                                                                                     cGGTGTCCAGATACAGTTCCATGATGTTTCC‑TTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCGGCGTTAACCAGCGCGTTACGGAATTCTTCGTGCATGATGCGGCGAGCGAGGCGCGAGAAAATgcgc                            >  2:224309/1‑150 (MQ=255)
                                                                                                                             aGATACAGTTCCATGATGTTTCC‑TTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCGGCGTTAACCAGCGCGTTACGGAATTCTTCGTGCATGATGCGGCGAGCGAGGCGCGAGAAAATGCGCATATGCTg                    >  1:137965/1‑150 (MQ=255)
                                                                                                                                tACAGTTCCATGATGTTTCC‑TTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCGGCGTTAACCAGCGCGTTACGGAATTCTTCGTGCATGATGCGGCGAGCGAGGCGCGAGAAAATGCGCATATGCTGATCg                >  1:42921/1‑151 (MQ=255)
                                                                                                                                       ccATGATGTTTCC‑TTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCGGCGTTAACCAGCGCGTTACGGAATTCTTCGTGCATGATGCGGCGAGCGAGGCGCGAGAAAATGCGCATATGCTGATCGCCCGc           >  2:402056/1‑149 (MQ=255)
                                                                                                                                           gatgTTTCC‑TTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCGGCGTTAACCAGCGCGTTACGGAATTCTTCGTGCATGATGCGGCGAGCGAGGCGCGAGAAAATGCGCATATGCTGATCGCCCGCAGCgt      <  2:647288/150‑1 (MQ=255)
                                                                                                                                            atgTTTCC‑TTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCGGCGTTAACCAGCGCGTTACGGAATTCTTCGTGCATGATGCGGCGAGCGAGGCGCGAGAAAATGCGCATATGCTGATCGCCCGCAGCgtgt    <  2:101715/151‑1 (MQ=255)
                                                                                                                                              gTTTCC‑TTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCGGCGTTAACCAGCGCGTTACGGAATTCTTCGTGCATGATGCGGCGAGCGAGGCGCGAGAAAATGCGCATATGCTGATCGCCCGCAGCGtgttt  <  1:249680/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GCAGACGCGGCAGCACTTCCCATATGGACTCCTTGCTGGCAGCGATAATGCTCGGGTTAGTTGTCACCCCGGCAATGGGGAATATGCGTGCCAGACGTTCGACTTCTGCGACGTTAGCGGTGTCCAGATACAGTTCCATGATGTTTCCTTTTACAGTTCCAGTTCATGTTGCAGCAGGCTGGCGATAGCGTCGGCAGAGGCGGCGTTAACCAGCGCGTTACGGAATTCTTCGTGCATGATGCGGCGAGCGAGGCGCGAGAAAATGCGCATATGCTGATCGCCCGCAGCGTGTTT  >  NC_000913/4139570‑4139863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A