Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,334,049	T→C	Q514Q (CAA→CAG)	eptA ←	lipid A phosphoethanolamine transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,334,049	0	T	C	100.0%	186.9 / NA	50	Q514Q (CAA→CAG)	eptA	lipid A phosphoethanolamine transferase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (28/22); total (28/22)

CAGTCCCTGCAATAACAGCGTATCGTCTTCAACAATCAGAATTTTCATTCACTCACTCTCCTGCAAGTTTGCAGAATATCATCCGCAGCCTGGTAATACTTCGTCTCAACGCCAGTTAATCCCAATAGCGTGGAGAATAAATTGTCTTGTGAATAGTGTTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGCCACAGCAGCATCGGCACCTGTTTTTGGCTATCCGGGGCGATGGCATAAGGCAGACCGTGC  >  NC_000913/4333903‑4334190

cAGTCCCTGCAATAACAGCGTATCGTCTTCAACAATCAGAATTTTCATTCACTCACTCTCCTGCAAGTTTGCAGAATATCATCCGCAGCCTGGTAATACTTCGTCTCAACGCCAGTTAATCCCAATAGCGTGGAGAATAAATTGTCCtgt                                                                                                                                            <  2:40992/150‑1 (MQ=255)

     ccTGCAATAACAGCGTATCGTCTTCAACAATCAGAATTTTCATTCACTCACTCTCCTGCAAGTTTGCAGAATATCATCCGCAGCCTGGTAATACTTCGTCTCAACGCCAGTTAATCCCAATAGCGTGGAGAATAAATTGTCCTGTGAATAg                                                                                                                                      <  1:676277/151‑1 (MQ=255)

       tGCAATAACAGCGTATCGTCTTCAACAATCAGAATTTTCATTCACTCACTCTCCTGCAAGTTTGCAGAATATCATCCGCAGCCTGGTAATACTTCGTCTCAACGCCAGTTAATCCCAATAGCGTGGAGAATAAATTGTCCTGTGAATAgtg                                                                                                                                    >  1:571639/1‑151 (MQ=255)

        gCAATAACAGCGTATCGTCTTCAACAATCAGAATTTTCATTCACTCACTCTCCTGCAAGTTTGCAGAATATCATCCGCAGCCTGGTAATACTTCGTCTCAACGCCAGTTAATCCCAATAGCGTGGAGAATAAATTGTCCTGTGAATAgt                                                                                                                                     <  1:462677/149‑1 (MQ=255)

          aaTAACAGCGTATCGTCTTCAACAATCAGAATTTTCATTCACTCACTCTCCTGCAAGTTTGCAGAATATCATCCGCAGCCTGGTAATACTTCGTCTCAACGCCAGTTAATCCCAATAGCGTGGAGAATAAATTGTCCTGTGAATAGTGtt                                                                                                                                  >  2:268216/1‑150 (MQ=255)

          aaTAACAGCGTATCGTCTTCAACAATCAGAATTTTCATTCACTCACTCTCCTGCAAGTTTGCAGAATATCATCCGCAGCCTGGTAATACTTCGTCTCAACGCCAGTTAATCCCAATAGCGTGGAGAATAAATTGTCCTGTGAATAGTGTTg                                                                                                                                 <  2:390495/151‑1 (MQ=255)

             aaCAGCGTATCGTCTTCAACAATCAGAATTTTCATTCACTCACTCTCCTGCAAGTTTGCAGAATATCATCCGCAGCCTGGTAATACTTCGTCTCAACGCCAGTTAATCCCAATAGCGTGGAGAATAAATTGTCCTGTGAATAGTGTTGCGt                                                                                                                              >  2:637865/1‑151 (MQ=255)

                aGCGTATCGTCTTCAACAATCAGAATTTTCATTCACTCACTCTCCTGCAAGTTTGCAGAATATCATCCGCAGCCTGGTAATACTTCGTCTCAACGCCAGTTAATCCCAATAGCGTGGAGAATAAATTGTCCTGTGAATAGTGTTGCGttt                                                                                                                            >  2:87533/1‑150 (MQ=255)

                aGCGTATCGTCTTCAACAATCAGAATTTTCATTCACTCACTCTCCTGCAAGTTTGCAGAATATCATCCGCAGCCTGGTAATACTTCGTCTCAACGCCAGTTAATCCCAATAGCGTGGAGAATAAATTGTCCTGTGAATAGTGTTGCGTTTg                                                                                                                           >  1:555075/1‑151 (MQ=255)

                  cgaaTCGTCTTCAACAATCAGAATTTTCATTCACTCACTCTCCTGCAAGTTTGCAGAATATCATCCGCAGCCTGGTAATACTTCGTCTCAACGCCAGTTAATCCCAATAGCGTGGAGAATAAATTGTCCTGTGAATAGTGTTGCGTTTgcg                                                                                                                         <  2:554105/148‑1 (MQ=255)

                       cGTCTTCAACAATCAGAATTTTCATTCACTCACTCTCCTGCAAGTTTGCAGAATATCATCCGCAGCCTGGTAATACTTCGTCTCAACGCCAGTTAATCCCAATAGCGTGGAGAATAAATTGTCCTGTGAATAGTGTTGCGTTTGCGCCTGt                                                                                                                    <  2:71722/151‑1 (MQ=255)

                         tCTTCAACAATCAGAATTTTCATTCACTCACTCTCCTGCAAGTTTGCAGCATATCATCCGCAGCCTGGTAATACTTCGTCTCAACGCCAGTTAATCCCAATAGCGTGGAGAATAAATTGTCCTGTGAATAGTGTTGCGTTTGCGCCTGttt                                                                                                                  >  2:595732/1‑151 (MQ=255)

                             caacaaTCAGAATTTTCATTCACTCACTCTCCTGCAAGTTTGCAGAATATCATCCGCAGCCTGGTAATACTTCGTCTCAACGCCAGTTAATCCCAATAGCGTGGAGAATAAATTGTCCTGTGAATAGTGTTGCGTTTGCGCCTGTTTTTgc                                                                                                              <  2:415500/151‑1 (MQ=255)

                              aacaaTCAGAATTTTCATTCACTCACTCTCCTGCAAGTTTGCAGAATATCATCCGCAGCCTGGTAATACTTCGTCTCAACGCCAGTTAATCCCAATAGCGTGGAGAATAAATTGTCCTGTGAATAGTGTTGCGTTTGCGCCTGTTTTTgca                                                                                                             <  1:121539/151‑1 (MQ=255)

                                    cAGAATTTTCATTCACTCACTCTCCTGCAAGTTTGCAGAATATCATCCGCAGCCTGGTAATACTTCGTCTCAACGCCAGTTAATCCCAATAGCGTGGAGAATAAATTGTCCTGTGAATAGTGTTGCGTTTGCGCCTGTTTTTGCAGGCAGt                                                                                                       >  1:619254/1‑151 (MQ=255)

                                     aGAATTTTCATTCACTCACTCTCCTGCAAGTTTGCAGAATATCATCCGCAGCCTGGTAATACTTCGTCTCAACGCCAGTTAATCCCAATAGCGTGGAGAATAAATTGTCCTGTGAATAGTGTTGCGTTTGCGCCTGTTTTTGCAGGCAGtt                                                                                                      >  2:125631/1‑151 (MQ=255)

                                          tttCATTCACTCACTCTCCTGCAAGTTTGCAGAATATCATCCGCAGCCTGGTAATACTTCGTCTCAACGCCAGTTAATCCCAATAGCGTGGAGAATAAATTGTCCTGTGAATAGTGTTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGt                                                                                                 >  1:155008/1‑151 (MQ=255)

                                          tttCATTCACTCACTCTCCTGCAAGTTTGCAGAATATCATCCGCAGCCTGGTAATACTTCGTCTCAACGCCAGTTAATCCCAATAGCGTGGAGAATAAATTGTCCTGTGAATAGTGTTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGt                                                                                                 >  2:173292/1‑151 (MQ=255)

                                             cattcaCTCACTCTCCTGCAAGTTTGCAGAATATCATCCGCAGCCTGGTAATACTTCGTCTCAACGCCAGTTAATCCCAATAGCGTGGAGAATAAATTGTCCTGTGAATAGTGTTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCa                                                                                               >  2:9328/1‑150 (MQ=255)

                                             cattcaCTCACTCTCCTGCAAGTTTGCAGAATATCATCCGCAGCCTGGTAATACTTCGTCTCAACGCCAGTTAATCCCAATAGCGTGGAGAATAAATTGTCCTGTGAATAGTGTTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCa                                                                                               <  1:7885/150‑1 (MQ=255)

                                                tcactcacTCTCCTGCAAGTTTGCAGAATATCATCCGCAGCCTGGTAATACTTCGTCTCAACGCCAGTTAATCCCAATAGCGTGGAGAATAAATTGTCCTGTGAATAGTGTTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAAcc                                                                                            <  1:593228/150‑1 (MQ=255)

                                                   ctcactcTCCTGCAAGTTTGCAGAATATCATCCGCAGCCTGGTAATACTTCGTCTCAACGCCAGTTAATCCCAATAGCGTGGAGAATAAATTGTCCTGTGAATAGTGTTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGa                                                                                         <  1:134703/150‑1 (MQ=255)

                                                    tcactcTCCTGCAAGTTTGCAGAATATCATCCGCAGCCTGGTAATACTTCGTCTCAACGCCAGTTAATCCCAATAGCGTGGAGAATAAATTGTCCTGTGAATAGTGTTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGAt                                                                                        >  2:28649/1‑150 (MQ=255)

                                                     cactcTCCTGCAAGTTTGCAGAATATCATCCGCAGCCTGGTAATACTTCGTCTCAACGCCAGTTAATCCCAATAGCGTGGAGAATAAATTGTCCTGTGAATAGTGTTGCGTTTGCGCCTGTTTTTTCAGGCAGTTCTGGTCAACCTGAt                                                                                        <  1:272557/149‑1 (MQ=255)

                                                       ctctCCTGCAAGTTTGCAGAATATCATCCGCAGCCTGGTAATACTTCGTCTCAACGCCAGTTAATCCCAATAGCGTGGAGAATAAATTGTCCTGTGAATAGTGTTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATACCg                                                                                    >  1:448127/1‑151 (MQ=255)

                                                          tcCTGCAAGTTTGCAGAATATCATCCGCAGCCTGGTAATACTTCGTCTCAACGCCAGTTAATCCCAATAGCGTGGAGAATAAATTGTCCTGTGAATAGTGTTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATACCGttt                                                                                 >  2:615792/1‑151 (MQ=255)

                                                           ccTGCAAGTTTGCAGAATATCATCCGCAGCCTGGTAATACTTCGTCTCAACGCCAGTTAATCCCAATAGCGTGGAGAATAAATTGTCCTGTGAATAGTGTTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATACCGtttt                                                                                <  2:182470/151‑1 (MQ=255)

                                                              gCAAGTTTGCAGAATATCATCCGCAGCCTGGTAATACTTCGTCTCAACGCCAGTTAATCCCAATAGCGTGGAGAATAAATTGTCCTGTGAATAGTGTTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGa                                                                             <  2:12069/151‑1 (MQ=255)

                                                                aaGTTTGCAGAATATCATCCGCAGCCTGGTAATACTTCGTCTCAACGCCAGTTAATCCCAATAGCGTGGAGAATAAATTGTCCTGTGAATAGTGTTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGAt                                                                            >  1:637963/1‑150 (MQ=255)

                                                                  gTTTGCAGAATATCATCCGCAGCCTGGTAATACTTCGTCTCAACGCCAGTTAATCCCAATAGCGTGGAGAATAAATTGTCCTGTGAATAGTGTTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGATAAt                                                                         >  1:558640/1‑151 (MQ=255)

                                                                      gCAGAATATCATCCGCAGCCTGGTAATACTTCGTCTCAACGCCAGTTAATCCCAATAGCGTGGAGAATAAATTGTCCTGTGAATAGCGTTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGATAAtcctc                                                                     <  1:197975/151‑1 (MQ=255)

                                                                             atcatcCGCAGCCTGGTAATACTTCGTCTCAACGCCAGTTAATCCCAATAGCGTGGAGAATAAATTGTCCTGTGAATAGTGTTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATGCCGTTTTTGATAATCCTCCGACAg                                                               <  1:515703/150‑1 (MQ=255)

                                                                             atcatcCGCAGCCTGGTAATACTTCGTCTCAACGCCAGTTAATCCCAATAGCGTGGAGAATAAATTGTCCTGTGAATAGTGTTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGc                                                              >  2:132129/1‑151 (MQ=255)

                                                                                    gCAGCCTGGTAATACTTCGTCTCAACGCCAGTTAATCCCAATAGCGTGGAGAATAAATTGTCCTGTGAATAGTGTTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGCCAcagca                                                       <  1:114035/151‑1 (MQ=255)

                                                                                      aGCCTGGTAATACTTCGTCTCAACGCCAGTTAATCCCAATAGCGTGGAGAATAAATTGTCCTGTGAATAGTGTTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGCCACagcagc                                                     >  2:69832/1‑151 (MQ=255)

                                                                                      aGCCTGGTAATACTTCGTCTCAACGCCAGTTAATCCCAATAGCGTGGAGAATAAATTGTCCTGTGAATAGTGTTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGCCACagcagc                                                     <  2:38844/151‑1 (MQ=255)

                                                                                      aGCCTGGTAATACTTCGTCTCAACGCCAGTTAATCCCAATAGCGTGGAGAATAAATTGTCCTGTGAATAGTGTTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGCCACagcagc                                                     >  1:451349/1‑151 (MQ=255)

                                                                                         cTGGTAATACTTCGTCTCAACGCCAGTTAATCCCAATAGCGTGGAGAATAAATTGTCCTGTGAATAGTGTTGCGTTTCCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCAGACAGCCACagcagc                                                     >  2:405907/1‑148 (MQ=255)

                                                                                          tGGTAATACTTCGTCTCAACGCCAGTTAATCCCAATAGCGTGGAGAATAAATTGTCCTGTGAATAGTGTTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGCCACAGCAGCATCg                                                 >  1:393885/1‑151 (MQ=255)

                                                                                                tACTTCGTCTCAACGCCAGTTAATCCCAATAGCGTGGAGAATAAATTGTCCTGTGAATAGTGTTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGCCACAGCAGCATCGGCACCt                                           >  2:118919/1‑151 (MQ=255)

                                                                                                     cGTCTCAACGCCAGTTAATCCCAATAGCGTGGAGAATAAATTGTCCTGTGAATAGTGTTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGCCACAGCAGCATCGGCACCTGtttt                                      >  2:596665/1‑151 (MQ=255)

                                                                                                          cAACGCCAGTTAATCCCAATAGCGTGGAGAATAAATTGTCCTGTGAATAGTGTTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGCCACAGCAGCATCGGCACCTGTTTTTgg                                   <  2:122054/149‑1 (MQ=255)

                                                                                                          cAACGCCAGTTAATCCCAATAGCGTGGAGAATAAATTGTCCTGTGAATAGTGTTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGCCACAGCAGCATCGGCACCTGTTTTTGGCt                                 <  1:475481/151‑1 (MQ=255)

                                                                                                                      aTCCCAATAGCGTGGACAATAAATTGTCCTGTGAATAGTGTTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGCCACAGCAGCATCGGCACCTGTTTTTGGCTATCCGGGGCGAt                     <  2:417561/151‑1 (MQ=255)

                                                                                                                        cccAATAGCGTGGAGAATAAATTGTCCTGTGAATAGTGTTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGCCACAGCAGCATCGGCACCTGTTTTTGGCTATCCGGTGCGATg                    >  2:344750/1‑150 (MQ=255)

                                                                                                                         ccAATAGCGTGGAGAATAAATTGTCCTGTGAATAGTGTTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGCCACAGCAGCATCGGCACCTGTTTTTGGCTATCCGGGGCGATGGc                  <  2:592075/151‑1 (MQ=255)

                                                                                                                              aGCGTGGAGAATAAATTGTCCTGTGAATAGTGTTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGCCACAGCAGCATCGGCACCTGTTTTTGGCTATCCGGGGCGATGGCATa               >  1:243039/1‑149 (MQ=255)

                                                                                                                                       aaTAAATTGTCCTGTGAATAGTGTTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGCCACAGCAGCATCGGCACCTGTTTTTGGCTATCCGGGGCGATGGCATAAGGCAGACCGt    >  1:263769/1‑151 (MQ=255)

                                                                                                                                         tAAATTGTCCTGTGAATAGTGTTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGCCACAGCAGCATCGGCACCTGTTTTTGGCTATCCGGGGCGATGGCATAAGGCAGACCGTGc  >  2:175834/1‑151 (MQ=255)

                                                                                                                                          aaaTTGTCCTGTGAATAGTGTTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGCCACAGCAGCATCGGCACCTGTTTTTGGCTATCCGGGGCGATGGCATAAGGCAGACCGTGc  >  2:302659/1‑150 (MQ=255)

CAGTCCCTGCAATAACAGCGTATCGTCTTCAACAATCAGAATTTTCATTCACTCACTCTCCTGCAAGTTTGCAGAATATCATCCGCAGCCTGGTAATACTTCGTCTCAACGCCAGTTAATCCCAATAGCGTGGAGAATAAATTGTCTTGTGAATAGTGTTGCGTTTGCGCCTGTTTTTGCAGGCAGTTCTGGTCAACCTGATACCGTTTTTGATAATCCTCCGACAGCCACAGCAGCATCGGCACCTGTTTTTGGCTATCCGGGGCGATGGCATAAGGCAGACCGTGC  >  NC_000913/4333903‑4334190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A