Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,339,313	A→G	R403R (CGT→CGC)	adiA ←	arginine decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,339,313	0	A	G	100.0%	183.4 / NA	52	R403R (CGT→CGC)	adiA	arginine decarboxylase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (30/22); total (30/22)

ATCACTTCCTGTGTCAGTGACAGGCCGCTGTTGCCGTCCATCATCGACACCGCCACGTCGTTGGATGCGCAGATGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCACGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCATAGATCGGGTTGAAAC  >  NC_000913/4339170‑4339458

aTCACTTCCTGTGTCAGTGACAGGCCGCTGTTGCCGTCCATCATCGACACCGCCACGTCGTTGGATGCGCAGATGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCtt                                                                                                                                            <  2:303407/151‑1 (MQ=255)

       ccTGTGTCAGTGACAGGCCGCTGTTGCCGTCCATCATCGACACCGCCACGTCGTTGGATGCGCAGATGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTAc                                                                                                                                     >  2:294917/1‑151 (MQ=255)

          gtgtCAGTGACAGGCCGCTGTTGCCGTCTATCCTCGACACCGCCACGTCGTTGGATGCGCAGATGGCATACAGCGGGGAGGTGGTGGCATGCATCATGCAGGCCTGGTTGAAGCGGGAGAAGGTAATCGCCCCGCGACCTTCACGCACATg                                                                                                                                  >  2:241078/1‑151 (MQ=255)

          gtgtCAGTGACAGGCCGCTGTTGCCGTCCATCATCGACACCGCCACGTCGTTGGATGCGCAGATGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACa                                                                                                                                    <  2:418207/149‑1 (MQ=255)

           tgtCAGTGACAGGCCGCTGTTGCCGTCCATCATCGACACCGCCACGTCGTTGGATGCGCAGATGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATg                                                                                                                                  >  1:379354/1‑150 (MQ=255)

                  gACAGGCCGCTGTTGCCGTCCATCATCGACACCGCCACGTCGTTGGATGCGCAGATGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATaag                                                                                                                          <  2:668139/151‑1 (MQ=255)

                          gCTGTTGCCGTCCATCATCGACACCGCCACGTCGTTGGATGCGCAGATGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCtgt                                                                                                                  <  1:128743/151‑1 (MQ=255)

                                 ccGTCCATCATCGACACCGCCACGTCGTTGGATGCGCAGATGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAgcg                                                                                                           <  2:572785/151‑1 (MQ=255)

                                 ccGTCCATCATCGACACCGCCACGTCGTTGGATGCGCAGATGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAgc                                                                                                            <  2:596972/150‑1 (MQ=255)

                                          atcGACACCGCCACGTCGTTGGATGCGCAGATGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTcagc                                                                                                   >  1:13901/1‑150 (MQ=255)

                                            cgccacCGCCACGTCGTTGGATGCGCAGATGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGt                                                                                                <  1:7827/148‑1 (MQ=255)

                                            cGACACCGCCACGTCGTTGGATGCGCAGATGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGt                                                                                                <  2:460920/151‑1 (MQ=255)

                                               cacCGCCACGTCGTTGGATGCGCAGATGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTtg                                                                                             <  1:307926/151‑1 (MQ=255)

                                                acCGCCACGTCGTTGGATGCGCAGATGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTtgt                                                                                            <  2:429433/151‑1 (MQ=255)

                                                    ccACGTCGTTGGATGCGCAGATGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTgtgggt                                                                                        >  1:207624/1‑151 (MQ=255)

                                                        gtcgtTGGATGCGCAGATGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTgtgggtgg                                                                                      >  1:526337/1‑149 (MQ=255)

                                                          cgtTGGATGCGCAGATGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAgt                                                                                   <  2:359846/150‑1 (MQ=255)

                                                            ttGGATGCGCAGATGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCG‑CCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAgtgggt                                                                               >  1:103834/1‑151 (MQ=255)

                                                                 tGCGCAGATGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCg                                                                           >  2:175903/1‑151 (MQ=255)

                                                                      aGATGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAAc                                                                      >  1:369439/1‑151 (MQ=255)

                                                                        aTGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACgg                                                                    >  1:185965/1‑151 (MQ=255)

                                                                         tGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGt                                                                   >  2:558185/1‑151 (MQ=255)

                                                                          ggCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTa                                                                  <  1:283023/151‑1 (MQ=255)

                                                                             aTACAGCGGGGAGGTGGTGGCATTCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGCCCTTCACGTACCTGAATATAAGACGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGACAACGGt                                                                   >  2:501683/1‑147 (MQ=255)

                                                                                cAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCg                                                            >  1:450314/1‑151 (MQ=255)

                                                                                      ggAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTtgtg                                                       >  1:613936/1‑150 (MQ=255)

                                                                                       gAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGAt                                                     <  1:499323/151‑1 (MQ=255)

                                                                                         ggtggtGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCg                                                   >  1:171961/1‑151 (MQ=255)

                                                                                               ggCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGt                                             <  1:674473/151‑1 (MQ=255)

                                                                                                gcatgcagCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTGGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAgg                                              <  2:128198/149‑1 (MQ=255)

                                                                                                 catgcatCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTc                                           <  2:318566/151‑1 (MQ=255)

                                                                                                  atgcatCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGGTc                                           >  1:168948/1‑150 (MQ=255)

                                                                                                   tgcatCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTcgc                                         >  2:98282/1‑151 (MQ=255)

                                                                                                     catcatGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTcgccg                                       >  1:192817/1‑151 (MQ=255)

                                                                                                      atcatGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTcgccgc                                      <  1:175908/151‑1 (MQ=255)

                                                                                                        catGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTCGCCgcgc                                    >  1:617278/1‑151 (MQ=255)

                                                                                                          tGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTCGCCGCGCAt                                  >  2:494196/1‑151 (MQ=255)

                                                                                                                 cTGGTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTCGCCGCGCATGGCAt                             >  1:429351/1‑149 (MQ=255)

                                                                                                                   ggTTGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTCGCCGCGCATGGCATAg                           <  1:551395/149‑1 (MQ=255)

                                                                                                                     ttGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGAt                       >  2:187455/1‑151 (MQ=255)

                                                                                                                      tGAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGAt                       <  2:662434/150‑1 (MQ=255)

                                                                                                                       gAAGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCg                     >  1:609410/1‑151 (MQ=255)

                                                                                                                        aaGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCgg                    >  1:481575/1‑151 (MQ=255)

                                                                                                                        aaGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCgg                    <  1:478891/151‑1 (MQ=255)

                                                                                                                        aaGCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCgg                    >  1:23818/1‑151 (MQ=255)

                                                                                                                          gCGGGAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCTGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGAGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCg                     >  2:521809/1‑148 (MQ=255)

                                                                                                                             ggAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCAt                 >  1:284502/1‑149 (MQ=255)

                                                                                                                             ggAGAAGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCATAg               <  2:77514/151‑1 (MQ=255)

                                                                                                                                 aaGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCAt                 >  1:388115/1‑145 (MQ=255)

                                                                                                                                 aaGTTAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCATAGATCg           <  2:608193/151‑1 (MQ=255)

                                                                                                                                    ttAATCGCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCATAGATCGGGt        >  1:426674/1‑151 (MQ=255)

                                                                                                                                          gCCCCGCGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCATAGATCGGGTTGAAAc  >  2:411642/1‑151 (MQ=255)

ATCACTTCCTGTGTCAGTGACAGGCCGCTGTTGCCGTCCATCATCGACACCGCCACGTCGTTGGATGCGCAGATGGCATACAGCGGGGAGGTGGTGGCATGCATCATGTAGGCCTGGTTGAAGCGGGAGAAGTTAATCGCCCCACGACCTTCACGTACATGAATATAAGAAGCCTGTGACAGCGCATTCAGCAGTTTGTGGGTGGAGTGGGTGGCGAAAACGGTAGGACCGTTGTGATCGCCAGGTTCGCCGCGCATGGCATAGTGATCGGCATAGATCGGGTTGAAAC  >  NC_000913/4339170‑4339458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A