Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,341,617	A→T	D4E (GAT→GAA)	melR ←	melibiose operon transcriptional regulator; autoregulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,341,617	0	A	T	100.0%	118.9 / NA	31	D4E (GAT→GAA)	melR	melibiose operon transcriptional regulator; autoregulator
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (16/15); total (16/15)

GAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCGGGCTGCGGGTCTGTTTTTCGTCGCTGCTGCACATAAACGTATCTGTATTCATGGATGGCTCTCTTTCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTC  >  NC_000913/4341470‑4341760

gAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCGGGCTGCGGGTCTGTTTTTCGTCGCTGCTGCACATAAACGTTTCt                                                                                                                                              >  1:536997/1‑151 (MQ=255)

      cacaTCCCCTCCGCCCTGCCCATGCCAGTGGCTGGTGGGCATGATATGCGGTGCGCGAACCTCAATCTCCATCCGCTGGTATTCCGAATCCAGCGCCAGCGGGTTGCGGGTCTG‑TTTTCGTCGCTGCTGCACATAAACGTTTCTGTATTc                                                                                                                                        <  2:532962/150‑1 (MQ=255)

       acaTTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCGGGCTGCGGGTCTGTTTTTCGTCGCTGCTGCACATAAACGTTTCTGTATTCa                                                                                                                                       <  2:198868/151‑1 (MQ=255)

        caTTCACTTCGACCTGACCATGCCAGCGGCTGGTGGGCATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCGGGCTGCGGGTCTGTTTTTCGTCGCTGCTGCACATAAACGTTTCTGTATTCat                                                                                                                                      >  1:370609/1‑151 (MQ=255)

         aTTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCGGGCTGCGGGTCTGTTTTTCGTCGCTGCTGCACATAAACGTTTCTGTATTCatg                                                                                                                                     <  2:680147/151‑1 (MQ=255)

                                aGTGGCTGGTGGGCATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCGGGCTGCGGGTCTGTTTTTCGTCGCTGCTGCACATAAACGTTTCTGTATTCATGGATGGCTCTCTTTCCTGGAatat                                                                                                              <  1:214259/151‑1 (MQ=255)

                                 gTGGCTGGTGGGCATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCGGGCTGCGGGTCTGTTTTTCGTCGCTGCTGCACATAAACGTTTCTGTATTCATGGATGGCTCTCTTTCCTGGAATATc                                                                                                             <  1:499877/151‑1 (MQ=255)

                                     cTGGTGGGCATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCGGGCTGCGGGTCTGTTTTTCGTCGCTGCTGCACATAAACGTTTCTGTATTCATGGATGGCTCTCTTTCCTGGAATATCAGa                                                                                                          >  1:509632/1‑150 (MQ=255)

                                        gtggGCATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCGGGCTGCGGGTCTGTTTTTCGTCGCTGCTGCACATAAACGTTTCTGTATTCATGGATGGCTCTCTTTCCTGGAATATCAGAATTa                                                                                                      <  1:434147/151‑1 (MQ=255)

                                                        gTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCGGGCTGCGGGTCTGTTTTTCGTCGCTGCTGCACATAAACGTTTCTGTATTCATGGATGGCTCTCTTTCCTGGAATATCAGAATTATGGCAGGAGTgaggg                                                                                       >  1:262648/1‑150 (MQ=255)

                                                        gTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCGGGCTGCGGGTCTGTTTTTCGTCGCTGCTGCACATAAACGTTTCTGTATTCATGGATGGCTCTCTTTCCTGGAATATCAGAATTATGGCAGGAGTgaggg                                                                                       >  2:61476/1‑150 (MQ=255)

                                                             cgAAACTCAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCGGGCTGCGGGTCTGTTTTTCGTCGCTGCTGCACATAAACGTTTCTGTATTCATGGATGGCTCTCTTTCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATg                                                                                 >  1:346415/1‑151 (MQ=255)

                                                                   tCAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCGGGCTGCGGGTCTGTTTTTCGTCGCTGCTGCACATAAACGTTTCTGTATTCATGGATGGCTCTCTTTCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCg                                                                           >  1:219280/1‑151 (MQ=255)

                                                                    cAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCGGGCTGCGGGTCTGTTTTTCGTCGCTGCTGCACATAAACGTTTCTGTATTCATGGATGGCTCTCTTTCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGa                                                                          <  2:142196/151‑1 (MQ=255)

                                                                              ccGCTGGTATTCCGAATACAGCGACAGCGGGCTGCGGGTCTGTTTTTCGTCGCTGCTGAACATAAACGTTTCTGTATTCATGGATGGCTCTCTTTCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCAc                                                                >  1:286073/1‑151 (MQ=255)

                                                                                gCTGGTATTCCGAATACAGCGACAGCGGGCTGCGGGTCTGTTTTTCGTCGCTGCTGCACATAAACGTTTCTGTATTCATGGATGGCTCTCTTTCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACgg                                                              >  2:54104/1‑151 (MQ=255)

                                                                                   ggTATTCCGAATACAGCGACAGCGGGCTGCGGGTCTGTTTTTCGTCGCTGCTGCACATAAACGTTTCTGTATTCATGGATGGCTCTCTTTCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGttt                                                           <  1:419667/151‑1 (MQ=255)

                                                                                     tATTCCGAATACAGCGACAGCGGGCTGCGGGTCTGTTTTTCGTCGCTGCTGCACATAAACGTTTCTGTATTCATGGATGGCTCTCTTTCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGtttt                                                          >  2:539967/1‑150 (MQ=255)

                                                                                            aaTACAGCGACAGCGGGCTGCGGGTCTGTTTTTCGTCGCTGCTGCACATAAACGTTTCTGTATTCATGGATGGCTCTCTTTCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCctct                                                  <  2:260010/151‑1 (MQ=255)

                                                                                                cagcgacagcgGGCTGCGGGTCTGTTTTTCGTCGCTGCTGCACATAAACGTTTCTGTATTCATGGATGGCTCTCTTTCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTccc                                              <  1:189849/151‑1 (MQ=255)

                                                                                                   cgacagcgGGCTGCGGGTCTGTTTTTCGTCGCTGCTGCACATAAACGTTTCTGTATTCATGGATGGCTCTCTTTCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTccc                                              >  1:329128/1‑148 (MQ=255)

                                                                                                      cagcgGGCTGCGGGTCTGTTTTTCGTCGCTGCTGCACATAAACGTTTCTGTATTCATGGATGGCTCTCTTTCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAggg                                        <  1:121019/151‑1 (MQ=255)

                                                                                                               gCGGGTCTGTTTTTCGTCGCTGCTGCACATAAACGTTTCTGTATTCATGGATGGCTCTCTTTCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAgggg                                >  2:642676/1‑150 (MQ=255)

                                                                                                               gCGGGTCTGTTTTTCGTCGCTGCTGCACATAAACGTTTCTGTATTCATGGATGGCTCTCTTTCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGa                               <  1:287109/151‑1 (MQ=255)

                                                                                                                 gggTCTGTTTTTCGTCGCTGCTGCACATAAACGTTTCTGTATTCATGGATGGCTCTCTTTCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGAct                             <  1:630002/151‑1 (MQ=255)

                                                                                                                         ttttCGTCGCTGCTGCACATAAACGTTTCTGTATTCATGGATGGCTCTCTTTCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGt                     >  1:44151/1‑151 (MQ=255)

                                                                                                                              gtcgCTGCTGCACATAAACGTTTCTGTATTCATGGATGGCTCTCTTTCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCAt                <  2:90591/151‑1 (MQ=255)

                                                                                                                                        cacaTAAACGTTTCTGTATTCATGGATGGCTCTCTTTCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCTAGGGGACTCTCCGAGTATCATGAGGCCGaa       >  1:201421/1‑150 (MQ=255)

                                                                                                                                         acaTAAACGTTTCTGTATTCATGGATGGCTCTCTTTCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGaaaa     <  1:624148/151‑1 (MQ=255)

                                                                                                                                         acaTAAACGTTTCTGTATTCATGGATGGCTCTCTTTCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGaaa      >  2:478712/1‑150 (MQ=255)

                                                                                                                                            tAAACGTTTCTGTATTCATGGATGGCTCTCTTTCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAActc  >  2:366989/1‑151 (MQ=255)

GAAAGGCACATTCACTTCGACCTGACCATGCCAGTGGCTGGTGGGCATGATATGCGGTGCGCGAAACTCAATCTCCATCCGCTGGTATTCCGAATACAGCGACAGCGGGCTGCGGGTCTGTTTTTCGTCGCTGCTGCACATAAACGTATCTGTATTCATGGATGGCTCTCTTTCCTGGAATATCAGAATTATGGCAGGAGTGAGGGAGGATGACTGCGAGTGGGAGCACGGTTTTCACCCTCTTCCCAGAGGGGCGAGGGGACTCTCCGAGTATCATGAGGCCGAAAACTC  >  NC_000913/4341470‑4341760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A