Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,415,887	(G)_6→7	coding (1613/1626 nt)	aidB →	DNA alkylation damage repair protein; flavin‑containing DNA binding protein, weak isovaleryl CoA dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,415,881	1	.	G	97.9%	167.5 / ‑2.5	48	coding (1607/1626 nt)	aidB	DNA alkylation damage repair protein; flavin‑containing DNA binding protein, weak isovaleryl CoA dehydrogenase
Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (24/23); total (25/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

CATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGAC‑GGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGT  >  NC_000913/4415733‑4416007

catcaGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGAC‑gg                                                                                                                              >  2:504332/1‑151 (MQ=255)

 atcaGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGAC‑ggg                                                                                                                             <  2:75400/151‑1 (MQ=255)

       tATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGAC‑ggggggg                                                                                                                         >  1:49621/1‑148 (MQ=255)

        aTTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCTAGAATGATTTATTGCTGCGGGCGAC‑ggggggga                                                                                                                        <  2:293205/149‑3 (MQ=255)

          tCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAgtgt                                                                                                                     >  2:401677/1‑151 (MQ=255)

           ccTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAgtgtg                                                                                                                    >  2:309836/1‑151 (MQ=255)

                      gCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCg                                                                                                          <  1:63296/150‑1 (MQ=255)

                         gTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGtata                                                                                                      <  2:308160/151‑1 (MQ=255)

                           gcgcAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACg                                                                                                    <  1:232633/151‑1 (MQ=255)

                            cgcAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGa                                                                                                   <  2:220261/151‑1 (MQ=255)

                              cAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACt                                                                                                 >  2:274227/1‑151 (MQ=255)

                               aaaTGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACt                                                                                                 <  1:107018/150‑1 (MQ=255)

                                 ctGTTGAAATATGCTTCTCCGCCAATGTCGCAGGCGTGGTGTCAGGTGATGTTAGATACGAGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTg                                                                                                >  2:244870/2‑149 (MQ=255)

                                   gTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGc                                                                                            <  1:312717/151‑1 (MQ=255)

                                   gTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGAC‑GGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCg                                                                                           >  1:226882/1‑151 (MQ=255)

                                     tGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCg                                                                                           <  1:135949/150‑1 (MQ=255)

                                       aaaTATGCTTCTGCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACg                                                                                        >  2:536673/1‑151 (MQ=255)

                                              cTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGttt                                                                                 >  2:5240/1‑151 (MQ=255)

                                                tctcCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGttt                                                                                 <  2:24879/149‑1 (MQ=255)

                                                tctcCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCg                                                                               >  2:95469/1‑151 (MQ=255)

                                                  tcCGCCAATGCAGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGACGGCGGACGGTTGTCAGAGCAGATCAAGAATGATTTATTGCTGAGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTAAACGACTGATGCGTCGCTGGTTTCGa                                                                              >  2:447612/1‑150 (MQ=255)

                                                     gCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTa                                                                           >  1:431740/1‑150 (MQ=255)

                                                       cAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACt                                                                        >  1:275616/1‑151 (MQ=255)

                                                         aTGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTaa                                                                      <  2:586020/151‑1 (MQ=255)

                                                           ggCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTaaa                                                                     <  2:279491/150‑1 (MQ=255)

                                                           ggCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGATGGTTTCGATTAACTAAAt                                                                    <  1:17863/151‑1 (MQ=255)

                                                               cAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGaa                                                                 >  2:147745/1‑150 (MQ=255)

                                                                           cAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAAt                                                     <  1:583063/150‑1 (MQ=255)

                                                                              gTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGt                                                  >  1:223928/1‑150 (MQ=255)

                                                                              gTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTa                                                 <  2:355832/151‑1 (MQ=255)

                                                                                gATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAgg                                               <  1:100485/151‑1 (MQ=255)

                                                                                gATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAg                                                <  2:642217/150‑1 (MQ=255)

                                                                                gATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAg                                                <  1:164900/150‑1 (MQ=255)

                                                                                        aTACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACa                                        >  2:465976/1‑150 (MQ=255)

                                                                                         tACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACa                                        >  2:125597/1‑149 (MQ=255)

                                                                                          aCGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAg                                      <  2:309785/150‑1 (MQ=255)

                                                                                                   ggcgTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTcgcg                             <  1:546310/150‑1 (MQ=255)

                                                                                                    gcgTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGcgcc                           >  1:502592/1‑151 (MQ=255)

                                                                                                        aCGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATGGAAGCCCGGACAAGGGGCCCGCGCGGCa                        >  2:309843/1‑150 (MQ=255)

                                                                                                               tCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCAt                >  2:4331/1‑151 (MQ=255)

                                                                                                                 agagCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTg              >  2:263795/1‑151 (MQ=255)

                                                                                                                 agagCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTg              <  2:269014/151‑1 (MQ=255)

                                                                                                                 agagCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTg              >  2:513216/1‑151 (MQ=255)

                                                                                                                 agagCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTg              <  2:354559/151‑1 (MQ=255)

                                                                                                                  gagCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGt             >  2:596417/1‑151 (MQ=255)

                                                                                                                    gCAGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTATGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGtt            <  1:144849/150‑1 (MQ=255)

                                                                                                                      aGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCAt         >  1:395918/1‑151 (MQ=255)

                                                                                                                      aGATCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCAt         <  1:48945/151‑1 (MQ=255)

                                                                                                                        aTCCAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCAttt       <  1:85530/151‑1 (MQ=255)

                                                                                                                           cAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCt    >  1:324379/1‑151 (MQ=255)

                                                                                                                           cAGAATGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCt    >  2:645292/1‑151 (MQ=255)

                                                                                                                                tGATTTATTGCTGCGGGCGACGGGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGt  >  2:666356/1‑148 (MQ=255)

CATCAGCTATTCCTGCTGGGCTGCGGTGCGCAAATGTTGAAATATGCTTCTCCGCCAATGGCGCAGGCGTGGTGTCAGGTGATGTTAGATACGCGCGGCGGCGTACGGTTGTCAGAGCAGATCCAGAATGATTTATTGCTGCGGGCGAC‑GGGGGGAGTGTGTGTGTAAGCGTATACGACTGATGCGACGCTGGTTTCGATTAACTAAATGAAATATGTGAAAATTGTAGGCCGGACAAGGCGCTCGCGCCGCATCCGGCATTGTTCATTTGCTGT  >  NC_000913/4415733‑4416007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A