Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F50 I2 R1	209	44.2	1360995	98.7%	1343302	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,485,819	C→T	A45T (GCC→ACC)	pepA ←	multifunctional aminopeptidase A: a cyteinylglycinase, transcription regulator and site‑specific recombination factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,485,819	0	C	T	98.0%	180.9 / ‑4.2	49	A45T (GCC→ACC)	pepA	multifunctional aminopeptidase A: a cyteinylglycinase, transcription regulator and site‑specific recombination factor
Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (27/21); total (28/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

AATAACCTGCTTGTACTGACGCTCATCCAGCTCACGTTCTTTGCCGCAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCGCCCCGACGTAGCAGGGCGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACT  >  NC_000913/4485673‑4485956

aaTAACCTGCTTGTACTGACGCTCATCCAGCTCACGTTCTTTGCCGCAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCGCCCCGACGTAGCAGGGTGCTg                                                                                                                                       >  1:150541/1‑151 (MQ=255)

aaTAACCTGCTTGTACTGACGCTCATCCAGCTCACGTTCTTTGCCGCAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCGCCCCGACGTAGCAGGGTGCTg                                                                                                                                       <  1:470006/151‑1 (MQ=255)

   aaCCTGCTTGTACTGACGCTCATCCAGCTCACGTTCTTTGCCGCAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCGCCCCGACGTAGCAGGGTGCTGAt                                                                                                                                     <  2:515100/150‑1 (MQ=255)

    aCCTGCTTGTACTGACGCTCATCCAGCTCACGTTCTTTGCCGCAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCGCCCCGACGTAGCAGGGTGCTGATGt                                                                                                                                   <  2:75914/151‑1 (MQ=255)

          ttGTACTGACGCTCATCCAGCTCACGTTCTTTGCCGCAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCGCCCCGACGTAGCAGGGTGCTGATGTACCCAt                                                                                                                             >  2:139976/1‑151 (MQ=255)

            gTACTGACGCTCATCCAGCTCACGTTCTTTGCCGCAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCGCCCCGACGTAGCAGGGTGCTGATGTACCCATc                                                                                                                            <  2:257424/150‑1 (MQ=255)

            gTACTGACGCTCATCCAGCTCACGTTCTTTGCCGCAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCGCCCCGACGTAGCAGGGTGCTGATGTACCCATCg                                                                                                                           <  2:304775/151‑1 (MQ=255)

             tACTGACGCTCATCCAGCTCACGTTCTTTGCCGCAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCGCCCCGACGTAGCAGGGTGCTGATGTACCCATCGc                                                                                                                          >  2:427945/1‑151 (MQ=255)

                    gCTCATCCAGCTCACGTTCTTTGCCGCAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCGCCCCGACGTAGCAGGGTGCTGATGTACCCATCGCTGAtttt                                                                                                                   >  2:68401/1‑151 (MQ=255)

                       cATCCAGCTCACGTTCTTTGCCGCAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCGCCCCGACGTAGCAGGGTGCTGATGTACCCATCGCTGATTTTa                                                                                                                  <  2:137012/149‑1 (MQ=255)

                        aTCCAGCTCACGTTCTTTGCCGCAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCGCCCCGACGTAGCAGGGTGCTGATGTACCCATCGCTGATTTTATCg                                                                                                               >  2:374936/1‑151 (MQ=255)

                        aTCCAGCTCACGTTCTTTGCCGCAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCGCCCCGACGTAGCAGGGTGCTGATGTACCCATCGCTGATTTTATCg                                                                                                               >  1:591271/1‑151 (MQ=255)

                         tCCAGCTCACGTTCTTTGCCGCAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCGCCCCGACGTAGCAGGGTGCTGATGTACCCATCGCTGATTTTATCg                                                                                                               >  1:156188/1‑150 (MQ=255)

                                cACGTTCTTTGCCGCAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCGCCCCGACGTAGCAGGGTGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTc                                                                                                       <  2:233230/151‑1 (MQ=255)

                                cACGTTCTTTGCCGCAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCGCCCCGACGTAGCAGGGTGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTc                                                                                                       >  1:103901/1‑151 (MQ=255)

                                 aCGTTCTTTGCCGCAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCGCCCCGACGTAGCAGGGTGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCt                                                                                                      >  2:655468/1‑151 (MQ=255)

                                  cGTTCTTTGCCGCAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCGCCCCGACGTAGCAGGGTGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTg                                                                                                     >  1:641864/1‑151 (MQ=255)

                                    ttCTTTGCCGCAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCGCCCCGACGTAGCAGGGTGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGc                                                                                                    >  1:645043/1‑150 (MQ=255)

                                        ttGCCGCAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCGCCCCGACGTAGCAGGGTGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATc                                                                                                <  1:19440/150‑1 (MQ=255)

                                        ttGCCGCAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCGCCCCGACGTAGCAGGGTGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATc                                                                                                <  2:268381/150‑1 (MQ=255)

                                         tGCCGCAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCGCCCCGACGTAGCAGGGTGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCgg                                                                                              >  1:233417/1‑151 (MQ=255)

                                           ccGCAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCGCCCCGACGTAGCAGGGTGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGgag                                                                                            <  1:226270/151‑1 (MQ=255)

                                            cGCAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCGCCCCGACGTAGCAGGGTGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCgg                                                                                              >  2:64651/1‑148 (MQ=255)

                                             gCAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCGCCCCGACGTAGCAGGGTGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGaa                                                                                          <  1:541832/151‑1 (MQ=255)

                                              cAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCGCCCCGACGTAGCAGGGTGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGaa                                                                                          >  2:470294/1‑150 (MQ=255)

                                                 ccAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCGCCCCGACGTAGCAGGGTGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGc                                                                                      >  2:460620/1‑151 (MQ=255)

                                                  cAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCGCCCCGACGTAGCAGGGTGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCg                                                                                     >  1:289651/1‑151 (MQ=255)

                                                            aaTTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCGCCCCGACGTAGCAGGGTGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCg                                                                           <  1:236647/151‑1 (MQ=255)

                                                                cGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCGCCCCGACGTAGCAGGGTGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGa                                                                          >  1:638195/1‑148 (MQ=255)

                                                                     ggAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCGCCCCGACGTAGCAGGGTGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGAAGACGccc                                                                  >  2:144144/1‑151 (MQ=255)

                                                                         aGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCGCCCCGACGTAGCAGGGTGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCacga                                                              <  1:350688/151‑1 (MQ=255)

                                                                          gTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCGCCCCGACGTAGCAGGGTGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCacgac                                                             <  1:536619/151‑1 (MQ=255)

                                                                             cATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCGCCCCGACGTAGCAGGGTGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGAt                                                          <  1:601454/151‑1 (MQ=255)

                                                                                     aaaTGGTGCAGCACCACTTTCGGCCCCTGTTTTCCTTCCTGTTCTCCTCGACGCACCAGGCCGCCGATGTACCCATCGCTGATTTTCTCTAGCTGTTCCCCACTCGGCGAACGGCGACGCGGACCGGAGACGcc                                                                   >  2:670363/3‑134 (MQ=255)

                                                                                         ggTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCGCCCCGACGTAGCAGGGTGCTGATGTACCCATCGCTGATTTTATCGCGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGCACTcc                                               <  1:125176/150‑1 (MQ=255)

                                                                                            gcagcaACAATGTCTGCCCCGGTTTTCCTTCCTGGTCGCCCCGACGTACCAGGGTGCTGATGTACTCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCATGACGATGCAGGCACTCCgat                                            >  1:500577/1‑148 (MQ=255)

                                                                                                 aacaaTGTCTGCCCCGGTTTTCCTTCCAGTTCGCCCCGACGTAGCAGGGTGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTtct                                      <  1:387906/151‑1 (MQ=255)

                                                                                                        tCTGCCCCGGTTTTCCTTCCAGTTCGCCCCGACGTAGCAGGGTGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCt                               >  1:90619/1‑151 (MQ=255)

                                                                                                              ccGGTTTTCCTTCCAGTTCGCCCCGACGTAGCAGGGTGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCt                         >  2:168880/1‑151 (MQ=255)

                                                                                                                ggTTTTCCTTCCAGTTCGCCCCGACGTAGCAGGGTGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCttt                       <  2:317912/151‑1 (MQ=255)

                                                                                                                   tttCCTTCCAGTTCGCCCCGACGTAGCAGGGTGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCttttt                     <  2:64876/150‑1 (MQ=255)

                                                                                                                      ccttccAGTTCGCCCCGACGTAGCAGGGTGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTacac                 >  1:8/1‑151 (MQ=255)

                                                                                                                      ccttccAGTTCGCCCCGACGTAGCAGGGTGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTacac                 >  1:606103/1‑151 (MQ=255)

                                                                                                                          ccAGTTCGCCCCGACGTAGCAGGGTGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTaa              >  2:137909/1‑150 (MQ=255)

                                                                                                                            aGTTCGCCCCGACGTAGCAGGGTGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACt           >  2:570994/1‑151 (MQ=255)

                                                                                                                            aGTTCGCCCCGACGTAGCAGGGTGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACt           <  2:373438/151‑1 (MQ=255)

                                                                                                                                cGCCCCGACGTAGCAGGGTGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCAt       <  2:72486/151‑1 (MQ=255)

                                                                                                                                cGCCCCGACGTAGCAGGGTGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCAt       >  2:79658/1‑151 (MQ=255)

                                                                                                                                     cGACGTAGCAGGGTGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACt  >  2:6817/1‑151 (MQ=255)

AATAACCTGCTTGTACTGACGCTCATCCAGCTCACGTTCTTTGCCGCAACCAATAAGGAGAATTCGCTCGGAAAGTACATTCGGAACATGGTGCAGCAACAATGTCTGCCCCGGTTTTCCTTCCAGTTCGCCCCGACGTAGCAGGGCGCTGATGTACCCATCGCTGATTTTATCGAGCTGTTCTGCAATCGGAGAAAGGCGACGTGGTTCGAAGACGCCCACGACGATGCAGGCACTCCGCTGTTTCTCCGGGCTACCGCTTTTTACACTAAACTCCATGCACT  >  NC_000913/4485673‑4485956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A