Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A7 F50 I2 R1
|
209 |
44.2 |
1360995 |
98.7% |
1343302 |
150.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,606,331 |
A→G |
noncoding (71/87 nt) |
leuQ ← |
tRNA‑Leu |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,606,331 | 0 | A | G | 94.7%
| 102.5
/ ‑3.2
| 40 | noncoding (71/87 nt) | leuQ | tRNA‑Leu |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (18/18); total (21/19) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01 |
TTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTT‑GGTGCGA‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAAT > NC_000913/4606183‑4606471
|
tgatatcgcctcgtttggtgcgaggGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTT‑GGTGCGA‑‑GGGGGGGGGCt > 2:289866‑M2/26‑151 (MQ=255)
tcgcctcgtttggtgcgaggGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTT‑GGTGCGA‑‑GGGGGGGGGCTTGAAc > 1:607080‑M2/21‑151 (MQ=255)
cctcgtttggtgcgaggGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTT‑GGTGCGA‑‑GGGGGGGGGCTTGAAcccc > 1:538170‑M2/18‑151 (MQ=255)
tggtgcgaggGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTT‑GGTGCGA‑‑GGGGGGGGGCTTGAACCCCCACGTcc > 1:179456‑M2/11‑151 (MQ=255)
tggtgcgaggGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTT‑GGTGCGA‑‑GGGGGGGGGCTGGAACCCCCACGTcc > 2:652661‑M2/11‑151 (MQ=255)
gtgcgaggGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTT‑GGTGCGAG‑GGGGGGGGGCTTGAACCCCCACGTcc < 2:150262‑M2/142‑1 (MQ=255)
gaggGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATGCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTT‑GGTGCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAgg < 1:157817‑M2/147‑1 (MQ=255)
ggGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTT‑GGTGCGAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAgg > 1:150302‑M2/3‑150 (MQ=255)
ggggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTT‑GGTGCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGacac > 1:457490/1‑151 (MQ=255)
ggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTT‑GGTGCGA‑‑GGGGGGGG‑CTTGAACCCCCACGTCCGTAAGCACACTAACAcc > 1:538627/1‑151 (MQ=255)
gAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTT‑GGTGCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAg < 1:635237/151‑1 (MQ=255)
cccACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCACTATCAACGTGGTTCTT‑GCTGCGA‑‑GGCGCGGTCCTTGAACCCCCACGTCCGTAAGGATACTTACTCCTGGAGCGAg > 2:60726/1‑150 (MQ=21)
cccACGTCCATAAGAACACTAACACCTGAAGCTAGCGCTTCTACCAATTCCGCCACCTTCGCCCAGTGCGAACAATATCAACGTGGTTTTT‑GGTGCGAGGGGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTa < 2:484964/151‑1 (MQ=25)
gTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTT‑GGTGCGAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGt > 1:639868/1‑151 (MQ=255)
aaCACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTT‑GGTGCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTc > 1:91516/1‑151 (MQ=255)
cacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTT‑GGTGCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg > 2:397561/1‑151 (MQ=255)
ggAAGCTGGCGCGTCTACCAATTCCGCAACCTTGGCCCAGTGCGGGCAATATCAACGGGGTTTTT‑GG‑GGGG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGc < 1:486698/149‑1 (MQ=18)
gcgTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTT‑GGTGCGAG‑GGGGGGGGGCTTGAACCCCAACTTCCGTAAGACCACTAACACCTGAAGCTAGGGGGTCTACCAATTCAGCAACCTTCGCATACCAt > 2:18231/1‑149 (MQ=21)
cgTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTT‑GGTGCGAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCaa < 1:501228/151‑1 (MQ=255)
tgtACCAATGCTGACAACGTCGGCAAGTGCCAGCAAAATCAACGTGGATTTT‑GGGGCGAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAAt < 2:349268/148‑1 (MQ=17)
ccAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTT‑GGTGCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTa < 1:218230/150‑1 (MQ=255)
ccAAGTCCGCCACCTTCGGCCAGTGCGAAGAAAGAAAACGTGGTTTTTGGGGGGGG‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAAt < 1:441627/146‑1 (MQ=17)
aTTCCGCCACCTTCGCCCAATGCGAGCAATATCAACGTGGTTTTT‑GGTGCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTaaaaa < 1:622087/151‑1 (MQ=255)
ccGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTT‑GGTGCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGa > 2:306122/1‑150 (MQ=255)
ccACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTT‑GGTGCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTg > 1:256888/1‑151 (MQ=255)
ttCGCCCAGTGCGAGCAATATCAACGTGGTTTTT‑GGTGCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTAcc > 1:88728/1‑151 (MQ=255)
cAGTGCGAGCAATATCAACGTGGTTTTT‑GGTGCGAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCAc < 2:668514/148‑1 (MQ=255)
aaTATAATCGTGGTTTTT‑GGTGCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCt < 2:353003/150‑1 (MQ=255)
aaTAACAACGTGGTTTTT‑GGTGCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTa < 2:203753/151‑1 (MQ=255)
atCAACGTGGTTTTT‑GGTGCGA‑‑GGGGGGGGGCTTGATCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGt < 1:226241/150‑1 (MQ=255)
atCAACGTGGTTTTT‑GGTGCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTg > 1:553165/1‑151 (MQ=255)
tCAACGTGGTTTTT‑GGTGCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTgg > 1:52269/1‑151 (MQ=255)
aaCGTGGTTTTT‑GGTGCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGtt < 1:542769/151‑1 (MQ=255)
aaCGTGGTTTTT‑GGTGCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGtt < 1:479137/151‑1 (MQ=255)
aaCGTGGTTTTT‑GGTGCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGtt > 2:148202/1‑151 (MQ=255)
taatt‑GGTGCGA‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGCCTCATTACTTTAAAAGAATTCCTACCGCGGAGGGGCATTCGAGTGGTTTTc > 2:21972/4‑147 (MQ=14)
tt‑GGTGCGA‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCtt < 2:580436/149‑1 (MQ=255)
ggTGCGA‑‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCg < 1:555898/151‑1 (MQ=255)
tGCGAG‑GGGGGGGGGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGt > 2:24259/1‑151 (MQ=255)
agggggggggGCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAAt < 1:658691/149‑1 (MQ=255)
|
TTTGATATCGCCTCGTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTT‑GGTGCGA‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGTTTTCAGCTTTTCGTCAAT > NC_000913/4606183‑4606471
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
