Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F50 I2 R1 150 68.2 2132745 98.7% 2105019 150.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,101,039 (C)7→8 coding (585/720 nt) yggN ← DUF2884 family putative periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,101,0321.C97.1% 253.0 / 1.1 70coding (592/720 nt)yggNDUF2884 family putative periplasmic protein
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base C (38/30);  total (39/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.28e-01

GTCTTAAAATAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑C‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTTCGGCGCGAACCTGAT  >  NC_000913/3100888‑3101175
                                                                                                                                                 |                                                                                                                                                
gTCTTAAAATAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑c‑ccccc                                                                                                                                           <  1:867292/151‑1 (MQ=255)
     aaaaTAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑c‑cccccccagg                                                                                                                                      >  2:378266/1‑147 (MQ=255)
     aaaaTAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑c‑cccccccagg                                                                                                                                      >  2:527972/1‑147 (MQ=255)
        aTAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCtt                                                                                                                                    >  2:554410/1‑151 (MQ=255)
          agagGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTcc                                                                                                                                  >  2:411205/1‑151 (MQ=255)
          agagGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTc                                                                                                                                   <  2:312726/150‑1 (MQ=255)
           gagGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTccc                                                                                                                                 >  2:314743/1‑151 (MQ=255)
           gagGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑C‑CCCCCCAGGCTTCCCa                                                                                                                                <  1:958468/151‑1 (MQ=255)
                 tattTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGcac                                                                                                                            >  2:110906/1‑150 (MQ=255)
                  attTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGcaca                                                                                                                           <  2:395812/150‑1 (MQ=255)
                       aaaTTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTc                                                                                                                        <  2:1041837/148‑1 (MQ=255)
                       aaaTTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTg                                                                                                                      <  1:615218/150‑1 (MQ=255)
                       aaaTTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGt                                                                                                                     >  2:625773/1‑151 (MQ=255)
                         aTTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTa                                                                                                                    >  2:772635/1‑150 (MQ=255)
                           tCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATg                                                                                                                 >  1:309198/1‑151 (MQ=255)
                            cccGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATgg                                                                                                                <  2:917094/151‑1 (MQ=255)
                              cGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGt                                                                                                              >  2:408644/1‑151 (MQ=255)
                              cGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGt                                                                                                              <  1:850687/151‑1 (MQ=255)
                                aCCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTa                                                                                                            <  2:293695/151‑1 (MQ=255)
                                   aGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTAccg                                                                                                         >  1:258712/1‑151 (MQ=255)
                                   aGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTAccg                                                                                                         >  2:541675/1‑151 (MQ=255)
                                       cTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACcgccgc                                                                                                     >  1:973442/1‑151 (MQ=255)
                                         ttGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACcgccgc                                                                                                     <  1:770533/149‑1 (MQ=255)
                                           gCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCtttt                                                                                                 >  1:571059/1‑151 (MQ=255)
                                            cGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTc                                                                                                <  2:55690/151‑1 (MQ=255)
                                             ggCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTc                                                                                                >  1:311469/1‑150 (MQ=255)
                                                 aTCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCAc                                                                                           >  2:802801/1‑151 (MQ=255)
                                                     tCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCg                                                                                         <  1:852173/149‑1 (MQ=255)
                                                     tCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCg                                                                                         <  2:766976/149‑1 (MQ=255)
                                                     tCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCg                                                                                         <  2:15597/149‑1 (MQ=255)
                                                        agagTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTc                                                                                    <  1:1010792/151‑1 (MQ=255)
                                                        agagTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTc                                                                                    <  2:766556/151‑1 (MQ=255)
                                                        agagTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTc                                                                                    <  1:628613/151‑1 (MQ=255)
                                                               caACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGccc                                                                              <  2:550275/150‑1 (MQ=255)
                                                               caACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCa                                                                             <  1:39193/151‑1 (MQ=255)
                                                                         aCAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTaa                                                                    <  1:995774/150‑1 (MQ=255)
                                                                         aCAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTaa                                                                    <  1:164674/150‑1 (MQ=255)
                                                                          cAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATg                                                                  <  2:304228/151‑1 (MQ=255)
                                                                           aaaCATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGc                                                                 >  2:178017/1‑151 (MQ=255)
                                                                              cATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGt                                                              >  1:112336/1‑151 (MQ=255)
                                                                               aTCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTc                                                             >  1:715000/1‑151 (MQ=255)
                                                                                tCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTcc                                                            >  2:467854/1‑151 (MQ=255)
                                                                                   ttGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTc                                                             <  1:762846/147‑1 (MQ=255)
                                                                                   ttGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCt                                                           <  2:997832/149‑1 (MQ=255)
                                                                                   ttGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGt                                                         >  1:758542/1‑151 (MQ=255)
                                                                                       cAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTaaaa                                                     <  1:210937/151‑1 (MQ=255)
                                                                                          aCTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTc                                                  <  2:611731/151‑1 (MQ=255)
                                                                                           ctgctgGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTcc                                                 >  1:136878/1‑151 (MQ=255)
                                                                                            tgctgGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTcc                                                 >  1:624441/1‑150 (MQ=255)
                                                                                            tgctgGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTcc                                                 >  2:678665/1‑150 (MQ=255)
                                                                                                 gAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCAt                                           >  2:214644/1‑151 (MQ=255)
                                                                                                  aaaTCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCAtt                                          >  2:460853/1‑151 (MQ=255)
                                                                                                   aaTCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTg                                         >  1:139057/1‑151 (MQ=255)
                                                                                                    aTCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGc                                        >  1:889511/1‑151 (MQ=255)
                                                                                                        ttttCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTg                                     <  2:403327/150‑1 (MQ=255)
                                                                                                          ttCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGAtt                                  >  2:760392/1‑151 (MQ=255)
                                                                                                              tGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACt                              >  1:109832/1‑151 (MQ=255)
                                                                                                                gtttttCCACACGGTTTGGATTGAGGATTGCAG‑CTCCCCCCAGGCTTCCCAGCACATTCTGTACTGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTa                             >  2:975309/2‑150 (MQ=255)
                                                                                                                cTTTTTCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTa                             >  1:1009546/1‑150 (MQ=255)
                                                                                                                    tgccACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTATAAGGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATCGCCTGATTCACTAAttt                         <  2:968472/148‑2 (MQ=255)
                                                                                                                     tCCACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTGCCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCGCCCATTGCCTGATTCACTAATTGc                        <  2:200674/150‑1 (MQ=255)
                                                                                                                      ccACTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTg                      >  2:579396/1‑151 (MQ=255)
                                                                                                                         cTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGcc                   >  2:893170/1‑151 (MQ=255)
                                                                                                                         cTCGGTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGcc                   >  2:937936/1‑151 (MQ=255)
                                                                                                                            ggTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCTCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTTc                >  1:237759/1‑151 (MQ=255)
                                                                                                                            ggTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGcc                   >  2:244751/1‑148 (MQ=255)
                                                                                                                            ggTTTGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTTc                <  2:315706/151‑1 (MQ=255)
                                                                                                                               ttGGATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTTCGgc             >  1:489895/1‑151 (MQ=255)
                                                                                                                                  gATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTTCGgcg            <  2:699572/149‑1 (MQ=255)
                                                                                                                                  gATTGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTTCGGcgcg          >  2:935789/1‑151 (MQ=255)
                                                                                                                                     tGAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTTCGGCGCGAAc       >  2:230462/1‑151 (MQ=255)
                                                                                                                                      gAGGATTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTTCGGCGCGAAc       <  2:664254/150‑1 (MQ=255)
                                                                                                                                          aTTGCAGCC‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTTCGGCGCGAACCTGAt  >  1:540633/1‑151 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GTCTTAAAATAGAGGATTATTTTAAATTCCCGACCAGGGCTTTGCGGCTATCTTCCAGAGTCACAACGCGGCTACAAACATCTTTGCCAAACTGCTGGAAATCTTTTTCCTGCTTTTTCCACTCGGTTTGGATTGAGGATTGCAG‑C‑CCCCCCAGGCTTCCCAGCACATTCTGTAATGGGTTACCGCCGCTTTTCAGCACCGCTTTCGCGCCCATTTCATTAATGCTGTCCTGTAAAATTCCACCCATTGCCTGATTCACTAATTGCTGGCCTTCGGCGCGAACCTGAT  >  NC_000913/3100888‑3101175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A