Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F50 I2 R1
|
150 |
68.2 |
2132745 |
98.7% |
2105019 |
150.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,606,215 |
(G)8→9 |
noncoding (72/87 nt) |
leuP ← |
tRNA‑Leu |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,606,207 | 1 | . | G | 90.9%
| 127.8
/ 5.8
| 44 | noncoding (80/87 nt) | leuP | tRNA‑Leu |
| Reads supporting (aligned to +/- strand): ref base . (3/1); new base G (19/21); total (22/22) |
| Fisher's exact test for biased strand distribution p-value = 6.07e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGT‑TTGGTGCGA‑‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTA > NC_000913/4606057‑4606351
|
cAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGT‑TTGGTGCGa < 2:37733/151‑1 (MQ=255)
aaGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGT‑TTGGTGCGA‑‑gg‑ggg < 1:1007589/151‑1 (MQ=255)
aaGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGT‑TTGGTGCGA‑‑gg‑ggg < 1:861496/151‑1 (MQ=255)
ggAAGTACAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGT‑TTGGTGCGA‑‑gg‑ggggg > 1:52375/1‑151 (MQ=255)
gAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGT‑TTGGTGCGA‑‑gg‑gggggg > 1:56265/1‑151 (MQ=255)
aaGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGT‑TTGGTGCGA‑‑gg‑ggggggg > 1:872426/1‑150 (MQ=255)
aaaGATAATTGGTGCGAGGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGT‑TTGGTGCGA‑‑gg‑gggggggact < 2:212927/151‑5 (MQ=255)
aaGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGT‑TTGGTGCGAG‑GG‑GGGGGGACTTg > 1:974085/1‑151 (MQ=255)
tGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACACTCACCTTACTTTTAATGATTTCCTCTCGTGGAGGGGCAT‑‑GG‑GGGGGGACTTCAAc > 2:370520/1‑146 (MQ=14)
ggTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGT‑TTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCa < 1:598884/150‑1 (MQ=255)
cGA‑GGGGGGAGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCTTCTTACTTTTATTGATATCGCTTCGT‑TTGGTGCGA‑‑GGCTGGGGGACTTGAACCCCCCCGTcc > 2:641365/1‑151 (MQ=21)
a‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGT‑TTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGt > 1:187063/1‑151 (MQ=255)
gggggACTTGAACCCCCACGTCCGTAAGGACACTAACGCCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGT‑TTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGa < 2:775281/151‑1 (MQ=255)
gACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGCCATCTTACTTTTTTTGATATCGCCTCGT‑TTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGAacac > 1:417931/1‑151 (MQ=255)
ttGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATATTACTTTTTTTGATATCTCCTCGT‑TTCGTGCGAG‑GG‑GGGGGGACTTTAACCCCGACGTCCGTACGAAGACTa > 2:358386/1‑150 (MQ=21)
tGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGT‑TTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGAACACTaa < 1:648072/150‑1 (MQ=255)
gAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGT‑TTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAac < 1:1055907/150‑1 (MQ=255)
cccccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTATTGATATCGTCTCGT‑TGGGTGCGA‑‑GGCGGGGGGACTTCAACTCCTACTTCCGAAATaattctcacccgt > 2:580374/1‑140 (MQ=18)
ccccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGT‑TTGGTGCGA‑‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGa < 1:1031074/151‑1 (MQ=255)
ccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGT‑TTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGaa < 1:85474/151‑1 (MQ=255)
cacctacGTAAGGAAACTACCACCTGAAGCTAGCGTGACTACCAATTCCGCCACCTGCGCACAGTCATCTTACTTTTTGTGATATCGCCTCGT‑TTGGTGCGAGGGG‑GGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCGGaa < 2:747763/145‑1 (MQ=37)
aaGGACACTAACACCTGAAGCTAGCGAGTCTACCAATTCCGCCGCCGACGAACAGTCATCTTACTTTTTTTGATATCGCCTCGT‑TTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGcgcg < 2:500805/150‑1 (MQ=37)
aaCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGT‑TTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAAt < 1:894263/150‑1 (MQ=255)
tGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGT‑TTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGcc > 1:820833/1‑150 (MQ=255)
tGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGT‑TTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGcc > 1:85680/1‑150 (MQ=255)
cgTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGT‑TTGGTGCGAG‑GG‑GGGGGGACTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGccc > 2:227580/1‑149 (MQ=255)
gTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGT‑TTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGt < 1:803076/151‑1 (MQ=255)
tACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGT‑TTGGTGCGAG‑GG‑GGGGGGACTTCAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCCCCCa > 2:176826/1‑146 (MQ=255)
aaTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGT‑TTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg < 2:650465/149‑1 (MQ=255)
aaTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGT‑TTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg > 1:650483/1‑149 (MQ=255)
aTTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGT‑TTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCaa < 1:1060247/151‑1 (MQ=255)
gCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGT‑TTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCa < 1:226666/151‑1 (MQ=255)
ccTCCGCACGGTCATCGTACCTTTTTTGATATCGCCTCGT‑TTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGAACACGAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCTCCTTCGCCCAGTGCGAGCAATATCAAc < 2:1066008/149‑1 (MQ=21)
cgtagaacaGTCAACTTACTTTTTTTGATATCGCCTCGT‑TTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCa < 2:353009/140‑1 (MQ=255)
tCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGT‑TTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGt > 1:830953/1‑151 (MQ=255)
tCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGT‑TTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGt > 1:667939/1‑151 (MQ=255)
cacaGTCATCTTACTTTTTTTGATATCGCCTCGT‑TTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGtttt < 2:820802/151‑1 (MQ=255)
acaGTCATCTTACTTTTTTTGATATCGCCTCGT‑TTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGttttt > 2:462446/1‑151 (MQ=255)
acaGTCATCTTACTTTTTTTGATATCGCATCGT‑TTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACg < 2:542268/143‑1 (MQ=255)
caGTCATCTTACTTTTTTTGATATCGCCTCGT‑TTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTg > 1:728028/1‑151 (MQ=255)
gTCATCTTACTTTTTTTGATATCGCCTCGT‑TTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTgg < 2:1029500/150‑1 (MQ=255)
cATCTTACTTTTTTTGATATCGCCTCGT‑TTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTg > 2:469137/1‑150 (MQ=255)
tACTTTTTTTGATATCGCCTCGT‑TTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCg < 1:30686/147‑1 (MQ=255)
tACTTTTTTTGATATCACCTCGT‑TTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgg < 2:509037/150‑1 (MQ=255)
aCTATTTTTGATATCGCCTCGT‑TTGGTTGGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGAACTCTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGa < 2:316370/147‑1 (MQ=21)
tttGATATCGCCTCGT‑TTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGAc > 1:84988/1‑151 (MQ=255)
ttGATATCGCCTCGT‑TTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCACTGCGAGCAATATCAACGAGGTTTTTGGTGCGAGGCGGGGGAc > 2:53830/1‑150 (MQ=255)
gATATCGCCTCGT‑TTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTg > 1:220824/1‑151 (MQ=255)
tatCGCCTCGT‑TTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGAGGTTTTTGGTGCGAGGGGGGGGACtt > 2:405046/1‑148 (MQ=255)
gT‑TTGGTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTCCGAGGCGCGGGACTTGAACCCCCACGt > 2:426076/1‑151 (MQ=255)
gTGCGAG‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTa < 2:6452/151‑1 (MQ=255)
|
CAAGAAAGGAAGTAAAGATAATTGGTGCGA‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGT‑TTGGTGCGA‑‑GG‑GGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTA > NC_000913/4606057‑4606351
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
