Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,891,465	T→C	N145S (AAC→AGC)	cysJ ←	sulfite reductase, alpha subunit, flavoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,891,465	0	T	C	100.0%	160.4 / NA	43	N145S (AAC→AGC)	cysJ	sulfite reductase, alpha subunit, flavoprotein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (17/26); total (17/26)

GGTATTCAACATCGGCATCGACACGGTCGAGCAGGCGTTCACCACCCAGTTCCGCCAGCTTGCTGTCGAAATCTTTCCCGGACTGGCAGAAAAATTCATAAGAGCTATCGCCGAGGCTAAACACGGCAAACGCGGTGTTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGTTCCCCTTCCCCTTGCGTTGACGTCACTACGATGAGCAGTTTTTCGCTGGCGATTTGTTTGAATTTATAGTCGCCCGCGT  >  NC_000913/2891328‑2891609

ggTATTCAACATCGGCATCGACACGGTCGAGCAGGCGTTCACCACCCAGTTCCGCCAGCTTGCTGTCGAAATCTTTCCCGGACTGGCAGAAAAATTCATAAGAGCTATCGCCGAGGCTAAACACGGCAAACGCGGTGCTTTCCAGCTTTg                                                                                                                                      <  1:103736/150‑1 (MQ=255)

   aTTCAACATCGGCATCGACACGGTCGAGCAGGCGTTCACCACCCAGTTCCGCCAGCTTGCTGTCGAAATCTTTCCCGGACTGGCAGAAAAATTCATAAGAGCTATCGCCGAGGCTAAACACGGCAAACGCGGTGCTTTCCAGCTTTGgcg                                                                                                                                   <  1:382902/150‑1 (MQ=255)

     tCAACATCGGCATCGACACGGTCGAGCAGGCGTTCACCACCCAGTTCCGCCAGCTTGCTGTCGAAATCTTTCCCGGACTGGCAGAAAAATTCATAAGAGCTATCGCCGAGGCTAAACACGGCAAACGCGGTGCTTTCCAGCTTTGGCGCt                                                                                                                                 <  2:144438/150‑1 (MQ=255)

       aaCATCGGCATCGACACGGTCGAGCAGGCGTTCACCACCCAGTTCCGCCAGCTTGCTGTCGAAATCTTTCCCGGACTGGCAGAAAAATTCATAAGAGCTATCGCCGAGGCTAAACACGGCAAACGCGGTGCTTTCCAGCTTTGGCGCtttt                                                                                                                              >  1:386085/1‑151 (MQ=255)

         cATCGGCATCGACACGGTCGAGCAGGCGTTCACCACCCAGTTCCGCCAGCTTGCTGTCGAAATCTTTCCCGGACTGGCAGAAAAATTCATAAGAGCTATCGCCGAGGCTAAACACGGCAAACGCGGTGCTTTCCAGCTTTGGCGCtttttt                                                                                                                            <  2:597059/151‑1 (MQ=255)

         cATCGGCATCGACACGGTCGAGCAGGCGTTCACCACCCAGTTCCGCCAGCTTGCTGTCGAAATCTTTCCCGGACTGGCAGAAAAATTCATAAGAGCTATCGCCGAGGCTAAACACGGCAAACGCGGTGCTTTCCAGCTTTGGCGCtttttt                                                                                                                            <  2:512177/151‑1 (MQ=255)

           tCGGCATCGACACGGTCGAGCAGGCGTTCACCACCCAGTTCCGCCAGCTTGCTGTCGAAATCTTTCCCGGACTGGCAGAAAAATTCATAAGAGCTATCGCCGAGGCTAAACACGGCAAACGCGGTGCTTTCCAGCTTTGGCGCTTTTTTgg                                                                                                                          >  2:54522/1‑151 (MQ=255)

                   gACACGGTCGAGCAGGCGTTCACCACCCAGTTCCGCCAGCTTGCTGTCGAAATCTTTCCCGGACTGGCAGAAAAATTCATAAGAGCTATCGCCGAGGCTAAACACGGCAAACGCGGTGCTTTCCAGCTTTGGCGCTTTTTTGGAGAACAgg                                                                                                                  >  1:139470/1‑151 (MQ=255)

                               cAGGCGTTCACCACCCAGTTCCGCCAGCTTGCTGTCGAAATCTTTCCCGGACTGGCAGAAAAATTCATAAGAGCTATCGCCGAGGCTAAACACGGCAAACGCGGTGCTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAgc                                                                                                      >  2:36473/1‑151 (MQ=255)

                                  gCGTTCACCACCCAGTTCCGCCAGCTTGCTGTCGAAATCTTTCCCGGACTGGCAGAAAAATTCATAAGAGCTATCGCCGAGGCTAAACACGGCAAACGCGGTGCTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGcgcg                                                                                                   <  1:36474/151‑1 (MQ=255)

                                  gCGTTCACCACCCAGTTCCGCCAGCTTGCTGTCGAAATCTTTCCCGGACTGGCAGAAAAATTCATAAGAGCTATCGCCGAGGCTAAACACGGCAAACGCGGTGCTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGcgcg                                                                                                   <  2:48535/151‑1 (MQ=255)

                                   cGTTCACCACCCAGTTCCGCCAGCTTGCTGTCGAAATCTTTCCCGGACTGGCAGAAAAATTCATAAGAGCTATCGCCGAGGCTAAACACGGCAAACGCGGTGCTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGa                                                                                                  <  2:139474/151‑1 (MQ=255)

                                      tCACCACCCAGTTCCGCCAGCTTGCTGTCGAAATCTTTCCCGGACTGGCAGAAAAATTCATAAGAGCTATCGCCGAGGCTAAACACGGCAAACGCGGTGCTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACg                                                                                                <  1:329902/150‑1 (MQ=255)

                                       caccacCCAGTTCCGCCAGCTTGCTGTCGAAATCTTTCCCGGACTGGCAGAAAAATTCATAAGAGCTATCGCCGAGGCTAAACACGGCAAACGCGGTGCTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACgg                                                                                               <  2:249067/150‑1 (MQ=255)

                                          caccCAGTTCCGCCAGCTTGCTGTCGAAATCTTTCCCGGACTGGCAGAAAAATTCATAAGAGCTATCGCCGAGGCTAAACACGGCAAACGCGGTGCTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGcttc                                                                                           >  1:3071/1‑151 (MQ=255)

                                              cAGTTCCGCCAGCTTGCTGTCGAAATCTTTCCCGGACTGGCAGAAAAATTCATAAGAGCTATCGCCGAGGCTAAACACGGCAAACGCGGTGCTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTcc                                                                                       >  1:245806/1‑151 (MQ=255)

                                                gTTCCGCCAGCTTGCTGTCGAAATCTTTCCCGGACTGGCAGAAAAATTCATAAGAGCTATCGCCGAGGCTAAACACGGCAAACGCGGTGCTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCcgg                                                                                     <  2:245864/151‑1 (MQ=255)

                                                   ccGCCAGCTTGCTGTCGAAATCTTTCCCGGACTGGCAGAAAAATTCATAAGAGCTATCGCCGAGGCTAAACACGGCAAACGCGGTGCTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCcggcg                                                                                   <  1:567293/150‑1 (MQ=255)

                                                        aGCTTGCTGTCGAAATCTTTCCCGGACTGGCAGAAAAATTCATAAGAGCTATCGCCGAGGCTAAACACGGCAAACGCGGTGCTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGttccc                                                                             <  1:221962/151‑1 (MQ=255)

                                                         gCTTGCTGTCGAAATCTTTCCCGGACTGGCAGAAAAATTCATAAGAGCTATCGCCGAGGCTAAACACGGCAAACGCGGTGCTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGttcccc                                                                            <  1:192436/151‑1 (MQ=255)

                                                              cTGTCGAAATCTTTCCCGGACTGGCAGAAAAATTCATAAGAGCTATCGCCGAGGCTAAACACGGCAAACGCGGTGCTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGtt                                                                                <  1:180422/142‑1 (MQ=255)

                                                              cTGTCGAAATCTTTCCCGGACTGGCAGAAAAATTCATAAGAGCTATCGCCGAGGCTAAACACGGCAAACGCGGTGCTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGtt                                                                                >  2:180423/1‑142 (MQ=255)

                                                                  cGAAATCTTTCCCGGACTGGCAGAAAAATTCATAAGAGCTATCGCCGAGGCTAAACACGGCAAACGCGGTGCTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGTTCCCCTTCCCCTTg                                                                   <  2:646609/151‑1 (MQ=255)

                                                                                   tGGCAGAAAAATTCATAAGAGCTATCGCCGAGGCTAAACACGGCAAACGCGGTGCTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGTTCCCCTTCCCCTTGCGTTGACGTCACTACg                                                   >  2:404952/1‑150 (MQ=255)

                                                                                    ggCAGAAAAATTCATAAGAGCTATCGCCGAGGCTAAACACGGCAAACGCGGTGCTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGTTCCCCTTCCCCTTGCGTTGACGTCACTACGAt                                                 <  2:128991/151‑1 (MQ=255)

                                                                                      cAGAAAAATTCATAAGAGCTATCGCCGAGGCTAAACACGGCAAACGCGGTGCTTTCCAGCGTTGGCGCTTTTTTGGAGAACAGGATCGTATGCAGCGCGACGGCTTCTTCCGGCGGTTCCCCTTCCCCTTGCGTTGTCGTCACTACGa                                                  <  1:179032/148‑1 (MQ=255)

                                                                                           aaaTTCATAAGAGCTATCGCCGAGGCTAAACACGGCAAACGCGGTGCTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGTTCCCCTTCCCCTTGCGTTGACGTCACTACGATGAGCAGt                                          <  2:535736/151‑1 (MQ=255)

                                                                                           aaaTTCATAAGAGCTATCGCCGAGGCTAAACACGGCAAACGCGGTGCTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGTTCCCCTTCCCCTTGCGTTGACGTCACTACGATGAGCAGt                                          <  2:456607/151‑1 (MQ=255)

                                                                                              ttCATAAGAGCTATCGCCGAGGCTAAACACGGCAAACGCGGTGCTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGTTCCCCTTCCCCTTGCGTTGACGTCACTACGATGAGCAGtttt                                       >  2:541564/1‑151 (MQ=255)

                                                                                                cATAAGAGCTATCGCCGAGGCTAAACACGGCAAACGCGGTGCTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGTTCCCCTTCCCCTTGCGTTGACGTCACTACGATGAGCAGttttt                                      <  1:626895/150‑1 (MQ=255)

                                                                                                      agCTATCGCCGAGGCTAAACACGGCAAACGCGGTGCTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGTTCCCCTTCCCCTTGCGTTGACGTCACTACGATGAGCAGTTTTTCGCTGGc                               >  1:31982/1‑151 (MQ=255)

                                                                                                       gCTATCGCCGAGGCTAAACACGGCAAACGCGGTGCTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGTTCCCCTTCCCCTTGCGTTGACGTCACTACGATGAGCAGTTTTTCGCTGGCg                              <  2:324199/151‑1 (MQ=255)

                                                                                                        cTATCGCCGAGGCTAAACACGGCAAACGCGGTGCTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGTTCCCCTTCCCCTTGCGTTGACGTCACTACGATGAGCAGTTTTTCGCTGGCg                              >  2:30486/1‑150 (MQ=255)

                                                                                                         tATCGCCGAGGCTAAACACGGCAAACGCGGGGCTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGCAACTTATGCAGCGCGACGGCTTCTTCCGGCGGTTCCCCTTCCCCTTGCGTTGACGTCACTACGATTGGCCGTTTTTCGCTGGc                               >  1:156211/1‑148 (MQ=255)

                                                                                                          aTCGCCGAGGCTAAACACGGCAAACGCGGTGCTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGTTCCCCTTCCCCTTGCGTTGACGTCACTACGATGAGCAGTTTTTCGCTGGCGAtt                           >  1:341103/1‑151 (MQ=255)

                                                                                                             gCCGAGGCTAAACACGGCAAACGCGGTGCTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGTTCCCCTTCCCCTTGCGTTGACGTCACTACGATGAGCAGTTTTTCGCTGGCGAtttgt                        >  1:543281/1‑151 (MQ=255)

                                                                                                              ccGAGGCTAAACACGGCAAACGCGGTGCTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGTTCCCCTTCCCCTTGCGTTGACGTCACTACGATGAGCAGTTTTTCGCTGGCGAtttgt                        <  1:647940/150‑1 (MQ=255)

                                                                                                               cGAGGCTAAACACGGCAAACGCGGTGCTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGTTCCCCTTCCCCTTGCGTTGACGTCACTACGATGAGCAGTTTTTCGCTGGCGAtttgttt                      >  1:467077/1‑151 (MQ=255)

                                                                                                                 aGGCTAAACACGGCAAACGCGGTGCTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGTTCCCCTTCCCCTTGCGTTGACGTCACTACGATGAGCAGTTTTTCGCTGGCGAtttgtttg                     >  1:651619/1‑150 (MQ=255)

                                                                                                                 aGGCTAAACACGGCAAACGCGGTGCTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGTTCCCCTTCCCCTTGCGTTGACGTCACTACGATGAGCAGTTTTTCGCTGGCGATTTGTTTGa                    <  1:386711/151‑1 (MQ=255)

                                                                                                                       aaCACGGCAAACGCGGTGCTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGTTCCCCTTCCCCTTGCGTTGACGTCACTACGATGAGCAGTTTTTCGCTGGCGATTTGTTTGAATTtat              <  2:590707/151‑1 (MQ=255)

                                                                                                                          acGGCAAACGCGGTGCTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGTTCCCCTTCCCCTTGCGTTGACGTCACTACGATGAGCAGTTTTTCGCTGGCGATTTGTTTGAATTTATAGt           <  2:245807/151‑1 (MQ=255)

                                                                                                                                   gcgGTGCTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGTTCCCCTTCCCCTTGCGTTGACGTCACTACGATGAGCAGTTTTTCGCTGGCGATTTGTTTGAATTTATAGTCGCCCGCGt  >  1:469962/1‑151 (MQ=255)

GGTATTCAACATCGGCATCGACACGGTCGAGCAGGCGTTCACCACCCAGTTCCGCCAGCTTGCTGTCGAAATCTTTCCCGGACTGGCAGAAAAATTCATAAGAGCTATCGCCGAGGCTAAACACGGCAAACGCGGTGTTTTCCAGCTTTGGCGCTTTTTTGGAGAACAGGAACTTATGCAGCGCGACGGCTTCTTCCGGCGGTTCCCCTTCCCCTTGCGTTGACGTCACTACGATGAGCAGTTTTTCGCTGGCGATTTGTTTGAATTTATAGTCGCCCGCGT  >  NC_000913/2891328‑2891609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A