Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,898,881	C→T	A180T (GCA→ACA)	ygcU ←	putative FAD‑linked oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,898,881	0	C	T	100.0%	128.8 / NA	34	A180T (GCA→ACA)	ygcU	putative FAD‑linked oxidoreductase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (24/10); total (24/10)

GGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCG  >  NC_000913/2898736‑2899026

ggggTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCa                                                                                                                                              <  1:342932/151‑1 (MQ=255)

         ttAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACg                                                                                                                                      >  2:285442/1‑150 (MQ=255)

         ttAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACATTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACg                                                                                                                                      >  1:187671/1‑150 (MQ=255)

          tAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGAc                                                                                                                                    >  1:143947/1‑151 (MQ=255)

           aaaGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGAcc                                                                                                                                   >  1:36654/1‑151 (MQ=255)

             aGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCat                                                                                                                                 <  1:591445/151‑1 (MQ=255)

               aTTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCatat                                                                                                                               <  1:270326/151‑1 (MQ=255)

                     aCTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCg                                                                                                                          >  2:186064/1‑150 (MQ=255)

                             ttCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCg                                                                                                                 >  1:581711/1‑151 (MQ=255)

                                 gTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGt                                                                                                                >  2:19107/1‑148 (MQ=255)

                                   gATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAgt                                                                                                           >  2:130879/1‑151 (MQ=255)

                                    atatAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAgtg                                                                                                          >  1:82165/1‑151 (MQ=255)

                                      ataGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGtgtg                                                                                                        >  1:509422/1‑151 (MQ=255)

                                             taTGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACt                                                                                                 >  1:127075/2‑151 (MQ=255)

                                              aTGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTg                                                                                                >  2:442317/1‑151 (MQ=255)

                                                               atgatgatgTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTg                                                                               <  2:512389/151‑1 (MQ=255)

                                                                tgatgatgTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTgcg                                                                                                                    <  2:79802/113‑1 (MQ=255)

                                                                tgatgatgTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTgcg                                                                                                                    >  1:79802/1‑113 (MQ=255)

                                                                 gatgatgTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTGCt                                                                             >  1:242578/1‑151 (MQ=255)

                                                                    gatgTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTGCTAc                                                                           <  2:360090/150‑1 (MQ=255)

                                                                     atgTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTGCTAcc                                                                          >  2:114501/1‑150 (MQ=255)

                                                                       gtgACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAgg                                                                       >  2:440564/1‑151 (MQ=255)

                                                                            gAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGgccgcc                                                                  >  2:120394/1‑151 (MQ=255)

                                                                                       ccAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTgcgc                                                         >  2:196283/1‑149 (MQ=255)

                                                                                          gCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGGCGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCg                                                    >  2:513746/1‑151 (MQ=255)

                                                                                                              tttAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATg                                 <  2:127078/150‑1 (MQ=255)

                                                                                                              tttAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATg                                 <  2:5187/150‑1 (MQ=255)

                                                                                                                     cGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCgtgg                         >  1:237209/1‑151 (MQ=255)

                                                                                                                      gtgtGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCgtggt                        >  2:200346/1‑151 (MQ=255)

                                                                                                                      gtgtGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCgtggt                        >  1:275039/1‑151 (MQ=255)

                                                                                                                        gtgACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGgtgt                      <  1:200345/151‑1 (MQ=255)

                                                                                                                          gACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTa                     >  1:8557/1‑150 (MQ=255)

                                                                                                                                     cAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACg         >  1:439232/1‑151 (MQ=255)

                                                                                                                                             aCTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAAcgcg  <  2:171928/150‑1 (MQ=255)

GGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCG  >  NC_000913/2898736‑2899026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A