Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,912,885	A→G	I256T (ATC→ACC)	relA ←	(p)ppGpp synthetase I/GTP pyrophosphokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,912,885	0	A	G	100.0%	138.3 / NA	38	I256T (ATC→ACC)	relA	(p)ppGpp synthetase I/GTP pyrophosphokinase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (18/20); total (18/20)

GGCGATAGTGAGTGTGCACTATCCCCAGTGCGGCATAGCAATCCTGTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGT  >  NC_000913/2912748‑2913027

ggCGATAGTGAGTGTGCACTATCCCCAGTGCGGCATAGCAATCCTGTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGgac                                                                                                                                    <  1:458377/150‑1 (MQ=255)

    aTAGTGAGTGTGCACTATCCCCAGTGCGGCATAGCAATCCTGTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCa                                                                                                                               <  2:355579/151‑1 (MQ=255)

      agtgagtgTGCACTATCCCCAGTGCGGCATAGCAATCCTGTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCAt                                                                                                                              <  2:177432/150‑1 (MQ=255)

      agtgagtgTGCACTATCCCCAGTGCGGCATAGCAATCCTGTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATa                                                                                                                             <  2:225714/151‑1 (MQ=255)

                          aGTGCGGCATAGCAATCCTGTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCtt                                                                                                         >  2:161683/1‑151 (MQ=255)

                                    aGCAATCCTGTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCAt                                                                                               >  1:262588/1‑151 (MQ=255)

                                             gTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAgcgc                                                                                       <  1:250246/150‑1 (MQ=255)

                                             gTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAgcgc                                                                                       <  1:28122/150‑1 (MQ=255)

                                               aaaCGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAgcgcgc                                                                                     <  1:446409/150‑1 (MQ=255)

                                               aaaCGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCTCa                                                                                    <  1:421017/151‑1 (MQ=255)

                                                  cGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGAt                                                                                 <  2:216159/151‑1 (MQ=255)

                                                   gCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATg                                                                                >  2:229426/1‑151 (MQ=255)

                                                   gCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGGGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCACCGCGCAGATg                                                                                >  1:544236/1‑151 (MQ=255)

                                                              aTACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAAct                                                                     >  1:470660/1‑151 (MQ=255)

                                                               tACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAActc                                                                    >  2:98991/1‑151 (MQ=255)

                                                                    aCCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCg                                                                >  1:340827/1‑150 (MQ=255)

                                                                    aCCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCg                                                                >  2:384038/1‑150 (MQ=255)

                                                                       gcacgcacATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGt                                                            <  2:351506/151‑1 (MQ=255)

                                                                         acgcacATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAg                                                          <  2:38528/151‑1 (MQ=255)

                                                                          cgcacATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAgt                                                         <  2:218320/151‑1 (MQ=255)

                                                                                tCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTcgc                                                   >  1:164283/1‑151 (MQ=255)

                                                                                tCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTcgc                                                   >  2:359723/1‑151 (MQ=255)

                                                                                 cAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTcgc                                                   <  1:98989/150‑1 (MQ=255)

                                                                                           atcaAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGc                                        <  2:417488/151‑1 (MQ=255)

                                                                                                  gCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACGATACACTTCCGCTTTAACGCCTTCAGCTTTAATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCa                                  >  2:523232/1‑150 (MQ=255)

                                                                                                     aGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATgca                              <  1:229426/151‑1 (MQ=255)

                                                                                                     aGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATgc                               >  2:78293/1‑150 (MQ=255)

                                                                                                      ggTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATgc                               >  1:274541/1‑149 (MQ=255)

                                                                                                        ttCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATgcagc                            >  1:113971/1‑150 (MQ=255)

                                                                                                                 tGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCa                   >  2:466011/1‑150 (MQ=255)

                                                                                                                  gCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCa                   <  2:323616/149‑1 (MQ=255)

                                                                                                                         aCGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGttt           <  2:365061/150‑1 (MQ=255)

                                                                                                                           gCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGt         >  2:284622/1‑150 (MQ=255)

                                                                                                                           gCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTa        <  2:475488/151‑1 (MQ=255)

                                                                                                                             cAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTAtt      <  1:424286/151‑1 (MQ=255)

                                                                                                                              aGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTc     >  2:151971/1‑151 (MQ=255)

                                                                                                                               gATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCg    <  1:276593/151‑1 (MQ=255)

                                                                                                                                 tGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGt  >  2:109562/1‑151 (MQ=255)

GGCGATAGTGAGTGTGCACTATCCCCAGTGCGGCATAGCAATCCTGTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGT  >  NC_000913/2912748‑2913027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A