Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,931,401	A→G	Q116R (CAG→CGG)	ygdG →	Ssb‑binding protein, misidentified as ExoIX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,931,401	0	A	G	100.0%	162.7 / NA	46	Q116R (CAG→CGG)	ygdG	Ssb‑binding protein, misidentified as ExoIX
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (19/27); total (19/27)

AGCGGGTCGACCGCCAATGCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACAGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAGAAACGTTGGCTGGATGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTT  >  NC_000913/2931255‑2931548

aGCGGGTCGACCGCCAATGCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGcc                                                                                                                                                 >  2:475480/1‑151 (MQ=255)

  cGGGTCGACCGCCAAGGCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCACCCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGc                                                                                                                                                  <  1:510503/148‑1 (MQ=255)

           cGCCAATGCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGc                                                                                                                                      <  2:252012/151‑1 (MQ=255)

             ccAATGCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCaa                                                                                                                                    <  2:656527/151‑1 (MQ=255)

               aaTGCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACg                                                                                                                                  >  1:254512/1‑151 (MQ=255)

                aTGCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACg                                                                                                                                  >  1:455553/1‑150 (MQ=255)

                  gCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGAt                                                                                                                                <  2:167266/150‑1 (MQ=255)

                  gCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCATGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGAtt                                                                                                                               <  2:245596/151‑1 (MQ=255)

                    cGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGt                                                                                                                             >  1:206068/1‑151 (MQ=255)

                     ggAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGtt                                                                                                                            <  1:460388/151‑1 (MQ=255)

                        agagTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCg                                                                                                                         <  1:424504/151‑1 (MQ=255)

                        agagTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCg                                                                                                                         <  1:485578/151‑1 (MQ=255)

                                  gacgaGATGCCTGCATTACGCGCCGCCTTTGAGTAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATaa                                                                                                                 >  2:564088/1‑149 (MQ=255)

                                   acgaGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAgg                                                                                                              <  2:244002/151‑1 (MQ=255)

                                   acgaGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAgg                                                                                                              >  2:198449/1‑151 (MQ=255)

                                    cgaGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGc                                                                                                             >  1:396433/1‑151 (MQ=255)

                                      agaTGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCt                                                                                                            >  2:457670/1‑150 (MQ=255)

                                      agaTGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTa                                                                                                           <  2:211794/151‑1 (MQ=255)

                                      agaTGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTa                                                                                                           <  2:104891/151‑1 (MQ=255)

                                       gaTGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTa                                                                                                           <  2:262889/150‑1 (MQ=255)

                                           ccTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCACCGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGt                                                                                                       <  1:416946/150‑1 (MQ=255)

                                                    cgcgCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACttt                                                                                             >  1:549145/1‑151 (MQ=255)

                                                          gccTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCg                                                                                        >  1:571230/1‑150 (MQ=255)

                                                              ttGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACa                                                                                     <  2:491468/149‑1 (MQ=255)

                                                                 aGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACg                                                                                >  2:566328/1‑151 (MQ=255)

                                                                   cAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGGCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACAtt                                                                                   >  1:658040/1‑146 (MQ=255)

                                                                       gcggcgTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCg                                                                          <  1:422334/151‑1 (MQ=255)

                                                                         ggcgTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACtt                                                                                              <  1:497519/129‑1 (MQ=255)

                                                                         ggcgTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACtt                                                                                              >  2:497521/1‑129 (MQ=255)

                                                                           cgTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGAt                                                                      <  1:564083/151‑1 (MQ=255)

                                                                                                 ggCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAGAAACGTTGGCTgg                                                <  1:475477/151‑1 (MQ=255)

                                                                                                        aaGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAGAAACGTTGGCTGGATGCGcc                                         >  1:69137/1‑151 (MQ=255)

                                                                                                          gCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCGCCGACATTACGTATTCGTGATTACTTCCAGAAACGTTGGCTGGATGCGCCAt                                       <  1:265292/151‑1 (MQ=255)

                                                                                                            cGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAGAAACGTTGGCTGGATGCGCCAttt                                     <  2:150008/151‑1 (MQ=255)

                                                                                                             gATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAGAAACGTTGGCTGGATGCGCCAttt                                     >  2:163873/1‑150 (MQ=255)

                                                                                                             gATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAGAAACGTTGGCTGGATGCGCCATTTa                                    <  1:342919/151‑1 (MQ=255)

                                                                                                              aTGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAGAAACGTTGGCTGGATGCGCCATTTAt                                   <  1:47690/151‑1 (MQ=255)

                                                                                                                gACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAGAAACGTTGGCTGGATGCGCCATTTATCg                                 <  2:69137/151‑1 (MQ=255)

                                                                                                                      gccgccACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAGAAACGTTGGCTGGATGCGCCATTTATCGATAAAg                           <  1:447869/151‑1 (MQ=255)

                                                                                                                              gCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAGAAACGTTGGCTGGATGCGCCATTTATCGATAAAGAATTTGGc                   <  1:112845/151‑1 (MQ=255)

                                                                                                                                tGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAGAAACGTTGGCTGGATGCGCCATTTATCGATAAAGAATTTGGCGt                 >  1:431591/1‑151 (MQ=255)

                                                                                                                                  gCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTTCTGTCAGTTACTTTCACCGCCATTACGTATTCGTGATTACTTCCAGAGACGTTGGCTGGATGCGCCATTTATCGATAAAGAATTTGGCGtt                <  1:489452/150‑1 (MQ=255)

                                                                                                                                        aaaGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAGAAACGTTGGCTGGATGCGCCATTTATCGa                                <  2:244143/128‑1 (MQ=255)

                                                                                                                                        aaaGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAGAAACGTTGGCTGGATGCGCCATTTATCGa                                >  1:244142/1‑128 (MQ=255)

                                                                                                                                        aaaGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAGAAACGTTGGCTGGATGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCgc         >  1:471791/1‑151 (MQ=255)

                                                                                                                                            tGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAGAAACGTTGGCTGGATGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCgcagc      >  2:520274/1‑150 (MQ=255)

                                                                                                                                               cacgGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAGAAACGTTGGCTGGATGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGtt  <  2:2386/151‑1 (MQ=255)

AGCGGGTCGACCGCCAATGCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACAGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAGAAACGTTGGCTGGATGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTT  >  NC_000913/2931255‑2931548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A