Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,007,381	T→C	S374P (TCC→CCC)	ygeW →	putative carbamoyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,007,381	0	T	C	100.0%	150.0 / NA	41	S374P (TCC→CCC)	ygeW	putative carbamoyltransferase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (21/20); total (21/20)

CCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTG  >  NC_000913/3007235‑3007527

cccTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCg                                                                                                                                                <  1:344899/151‑1 (MQ=255)

      aCATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCAAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGACGTATATCATCGCCGCGATGATCCTGCCCCGTAAAt                                                                                                                                           <  1:41479/150‑1 (MQ=255)

        aTGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACg                                                                                                                                        >  2:498914/1‑151 (MQ=255)

          gCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGc                                                                                                                                       <  1:575157/150‑1 (MQ=255)

                   gccAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGt                                                                                                                             >  2:608727/1‑151 (MQ=255)

                     cAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGc                                                                                                                           <  2:193371/151‑1 (MQ=255)

                                 gcggcgTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCa                                                                                                               <  1:362293/151‑1 (MQ=255)

                                  cggcgTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCaa                                                                                                              <  1:17674/151‑1 (MQ=255)

                                  cggcgTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCaa                                                                                                              <  2:574529/151‑1 (MQ=255)

                                      gTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAActgc                                                                                                          >  2:461310/1‑151 (MQ=255)

                                      gTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAActgc                                                                                                          <  1:458572/151‑1 (MQ=255)

                                         tCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAActgctg                                                                                                        >  1:584426/1‑150 (MQ=255)

                                          ccTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGaa                                                                                                      <  2:42543/151‑1 (MQ=255)

                                           cTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGaa                                                                                                      >  2:469069/1‑150 (MQ=255)

                                                aagaagGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAgc                                                                                                <  2:305806/151‑1 (MQ=255)

                                                aagaagGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAgc                                                                                                <  2:121180/151‑1 (MQ=255)

                                                    agGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCaa                                                                                            <  1:206618/151‑1 (MQ=255)

                                                    agGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCaa                                                                                            <  1:74609/151‑1 (MQ=255)

                                                        gAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAAc                                                                                        >  2:618807/1‑151 (MQ=255)

                                                          aGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAAcgc                                                                                      >  1:279808/1‑151 (MQ=255)

                                                             gACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTg                                                                                   <  2:388757/151‑1 (MQ=255)

                                                               cTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGa                                                                                  >  2:85703/1‑150 (MQ=255)

                                                                      cGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAAccc                                                                          >  1:392106/1‑151 (MQ=255)

                                                                            cGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCgg                                                                     <  2:363878/150‑1 (MQ=255)

                                                                                                  tacAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCctct                                              >  2:73910/1‑151 (MQ=255)

                                                                                                            ccAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCag                                      >  2:282256/1‑149 (MQ=255)

                                                                                                             cAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAgg                                   >  2:34369/1‑151 (MQ=255)

                                                                                                                  ggAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCa                                <  2:455216/149‑1 (MQ=255)

                                                                                                                   gAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCaaaa                             <  1:216287/151‑1 (MQ=255)

                                                                                                                     aGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAagg                           >  1:510035/1‑151 (MQ=255)

                                                                                                                     aGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAag                            >  2:197575/1‑150 (MQ=255)

                                                                                                                          tataTCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAaggatagg                      >  2:236641/1‑151 (MQ=255)

                                                                                                                           atatCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAaggatagg                      >  2:262686/1‑150 (MQ=255)

                                                                                                                             atcatcGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAaggataggat                    >  1:427543/1‑150 (MQ=255)

                                                                                                                                   gccgcGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCg             >  2:545741/1‑151 (MQ=255)

                                                                                                                                       cgATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGtttt         <  1:294528/151‑1 (MQ=255)

                                                                                                                                       cgATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGtttt         <  2:161659/151‑1 (MQ=255)

                                                                                                                                       cgATGAGCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCATCTGGTCGGCCCTCTATTGCCAGAGGCCAAAAGGATAGGATATGTCCGtttt         <  1:546682/151‑1 (MQ=255)

                                                                                                                                        gatgatCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTtc        >  2:106548/1‑151 (MQ=255)

                                                                                                                                             tCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCAtt   >  1:654257/1‑151 (MQ=255)

                                                                                                                                               cTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCAATg  >  2:230090/1‑150 (MQ=255)

CCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTG  >  NC_000913/3007235‑3007527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A