Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,091,787	A→G	G218G (GGA→GGG)	rsmE →	16S rRNA m(3)U1498 methyltransferase, SAM‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,091,787	0	A	G	92.1%	96.9 / ‑5.3	38	G218G (GGA→GGG)	rsmE	16S rRNA m(3)U1498 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (17/18); minor base C (0/3); total (17/21)
Fisher's exact test for biased strand distribution p-value = 2.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

ATCTTCACCCGCGCGCCAGTAACAGCATCAATACGTTGCCGTTACCGGTTGAACGCGTCCGCCTGCTGATTGGCCCGGAAGGCGGTTTATCGGCAGATGAAATTGCCATGACTGCCCGCTATCAATTTACTGATATCCTGTTGGGACCTCGCGTTTTGCGTACAGAGACAACTGCGCTCACCGCCATTACCGCGCTACAAGTACGATTTGGCGATTTGGGCTAACGGAGAAGAATAATGATCAAGCTCGGCATCGTGATGGACCCCATCGCAAACATCAACATCAAGAA  >  NC_000913/3091642‑3091930

aTCTTCACCCGCGCGCCAGTAACAGCATCAATACGTTGCCGTTACCGGTTGAACGCGTCCGCCTGCTGATTGGCCCGGAAGGCGGTTTATCGGCAGATGAAATTGCCATGACTGCCCGCTATCAATTTACTGATATCCTGTTGGGGCCTcg                                                                                                                                            >  2:55065/1‑151 (MQ=255)

    tCACCCGCGCGCCAGTAACAGCATCAATACGTTGCCGTTACCGGTTGAACGCGTCCGCCTGCTGATTGGCCCGGAAGGCGGTTTATCGGCAGATGAAATTGCCATGACTGCCCGCTATCAATTTACTGATATCCTGTTGGGGCCTcgc                                                                                                                                           <  2:542678/148‑1 (MQ=255)

                      cAGCATCAATACGTTGCCGTTACCGGTTGAACGCGTCCGCCTGCTGATTGGCCCGGAAGGCGGTTTATCGGCAGATGAAATTGCCATGACTGCCCGCTATCAATTTACTGATATCCTGTTGGGGCCTCGCGTTTTGCGTACAGAGACAACt                                                                                                                      <  1:98604/151‑1 (MQ=255)

                       aGCATCAATACGTTGCCGTTACCGGTTGAACGCGTCCGCCTGCTGATTGGCCCGGAAGGCGGTTTATCGGCAGATGAAATTGCCATGACTGCCCGCTATCAATTTACTGATATCCTGTTGGGGCCTCGCGTTTTGCGTACAGAGACAACTg                                                                                                                     <  1:126061/151‑1 (MQ=255)

                          aTCAATACGTTGCCGTTACCGGTTGAACGCGTCCGCCTGCTGATTGGCCCGGAAGGCGGTTTATCGGCAGATGAAATTGCCATGACTGCCCGCTATCAATTTACTGATATCCTGTTGGGGCCTCGCGTTTTGCGTACAGAGACAACTgcgc                                                                                                                  <  1:308349/151‑1 (MQ=255)

                            cAATACGTTGCCGTTACCGGTTGAACGCGTCCGCCTGCTGATTGGCCCGGAAGGCGGTTTATCGGCAGATGAAATTGCCATGACTGCCCGCTATCAATTTACTGATATCCTGTTGGGGCCTCGCGTTTTGCGTACAGAGACAACTGCGCt                                                                                                                 <  2:661112/150‑1 (MQ=255)

                            cAATACGTTGCCGTTACCGGTTGAACGCGTCCGCCTGCTGATTGGCCCGGAAGGCGGTTTATCGGCAGATGAAATTGCCATGACTGCCCGCTATCAATTTACTGATATCCTGTTGGGGCCTCGCGTTTTGCGTACAGAGACAACTGCGCt                                                                                                                 <  1:216067/150‑1 (MQ=255)

                            cAATACGTTGCCGTTACCGGTTGAACGCGTCCGCCTGCTGATTGGCCCGGAAGGCGGTTTATCGGCAGATGAAATTGCCATGACTGCCCGCTATCAATTTACTGATATCCTGTTGGGGCCTCGCGTTTTGCGTACAGAGACAACTGCGCTc                                                                                                                >  2:516701/1‑151 (MQ=255)

                            cAATACGTTGCCGTTACCGGTTGAACGCGTCCGCCTGCTGATTGGCCCGGAAGGCGGTTTATCGGCAGATGAAATTGCCATGACTGCCCGCTATCAATTTACTGATATCCTGTTGGGGCCTCGCGTTTTGCGTACAGAGACAACTGCGCTc                                                                                                                >  1:581015/1‑151 (MQ=255)

                             aaTACGTTGCCGTTACCGGTTGAACGCGTCCGCCTGCTGATTGGCCCGGAAGGCGGTTTATCGGCAGATGAAATTGCCATGACTGCCCGCTATCAATTTACTGATATCCTGTTGGGGCCTCGCGTTTTGCGTACAGAGACAACTGCGCTc                                                                                                                >  2:196579/1‑150 (MQ=255)

                              aTACGTTGCCGTTACCGGTTGAACGCGTCCGCCTGCTGATTGGCCCGGAAGGCGGTTTATCGGCAGATGAAATTGCCATGACTGCCCGCTATCAATTTACTGATATCCTGTTGGGGCCTCGCGTTTTGCGTACAGAGACAACTGCGCTCAc                                                                                                              >  1:373337/1‑151 (MQ=255)

                                    tGCCGTTACCGGTTGAACGCGTCCGCCTGCTGATTGGCCCGGAAGGCGGTTTATCGGCAGATGAAATTGCCATGACTGCCCGCTATCAATTTACTGATATCCTGTTGGGGCCTCGCGTTTTGCGTACAGAGACAACTGCGCTCACCGCCAt                                                                                                        >  2:176974/1‑151 (MQ=255)

                                            ccGGTTGAACGCGCCCGCCTGCTGTTTGCCCCGGAAGGCGGTTTATCGGCAGATGAAATTGCCATGCCTGCCCGCTATAAATTTACTGATACCCTGTTGGGCCCTCGCTTTTTGCTTACAGAGACACCTGCACTCACCGCCATTACcg                                                                                                   <  1:370123/148‑1 (MQ=255)

                                                   accgcgTCCGCCTGCTGATTGGCCGGGAGGGCGGTTTATCGGCAGATAAAATTGCCATGACTGCCCGCTATCAATTTCCTGATATCCTGTTGGGCCCTCGCGTTTTGCGTACAGAGACAACTGCGCTCACCGCCATTACCGCGCTACa                                                                                            <  1:524882/146‑1 (MQ=255)

                                                      gcgcCCGCCGGCTGATGGGCCGGGAAGGCGTTTTATCGGCAAATGAATTGCCCCTGCCGGCCCAATATCATTTTGCGGATATCCTGTTGGGGCCGCGGGTTTTGGGTACAGAGACACTTGCGCCCACCGCCATTACCGCGCTACAAGTAc                                                                                       <  1:94589/150‑1 (MQ=255)

                                                      gcgTCCGCCTGCTGATTGGCCCGGAAGGCGGTTTATCGGCAGATAAAATTGCCATGACTGCCCGCTATCATTTTACTGATATCCTGTTGGGGCCTCGCTTTTTGCGTACAGAGAAAACTGCGCTCACCGCCATTACCGCGCTACAAGTAc                                                                                       <  2:209172/150‑1 (MQ=255)

                                                        gTCCCCCTGCTGATTGGCCCGGAAGGCGGTTTATCGGCAGATGAAATTGCCATGACTGCCCGCTATCAATTTACTGATATCCTGTTGGGGCCTCGCGTTTTGCGAACAGAGACAACTGCGCTCACCGCCATTACCGCGCTACAAGTAcga                                                                                     <  1:148359/150‑1 (MQ=255)

                                                          ccGCCTGCTGATTGGCCCGGAAGGCGTTTTATCGGCAGATGAAATTCCCATGACTGCCCGCTATCAATTTACTGATATCCTGTTGGGGCCTCGCGTTTTGCGTACAGAGACAACTGCGCTCACCGCCATTACCGCGCTACAAGTAcgatt                                                                                   <  1:176973/150‑1 (MQ=255)

                                                                            ggAGGGCGGTTTAGCGAAAGAAAAATTTGACATACCCGCCCGCTTCCATTTTACGATTGCCCTTTTGGGGTCTGGCTTTTGGCGTGCAGAGAAAACGGCGCTCACCGCCATTACCGCGCTACAAGTACGATTTGGCGATTTGGGCTa                                                                    <  1:595761/147‑1 (MQ=255)

                                                                                ggCGGTTTATCGGCAGATGAAATTGCCATGACTGCCCGCTATCAATTTACTGATATCCTGTTGGGGCCTCGCGTTTTGCGTACAGAGACAACTGCGCTCACCGCCATTACCGCGCTACAAGTACGATTTGGCGATTTGGGCTAACGgaga                                                             >  2:173665/1‑150 (MQ=255)

                                                                                        aTCGGCAGATGAAATTGCCATGACTGCCCGCTATCAATTTACTGATATCCTGTTGGGGCCTCGCGTTTTGCGTACAGAGACAACTGCGCTCACCGCCATTACCGCGCTACAAGTACGATTTGGCGATTTGGGCTAACGGAGAAGAATAATg                                                    >  2:198320/1‑151 (MQ=255)

                                                                                        aTCGGCAGATGAAATTGCCATGACTGCCCGCTATCAATTTACTGATATCCTGTTGGGGCCTCGCGTTTTGCGTACAGAGACAACTGCGCTCACCGCCATTACCGCGCTACAAGTACGATTTGGCGATTTGGGCTAACGGAGAAGAATAATg                                                    >  2:231314/1‑151 (MQ=255)

                                                                                              aGATGAAATTGCCATGACTGCCCGCTATCAATTTACTGATATCCTGTTGGGGCCTCGCGTTTTGCGTACAGAGACAACTGCGCTCACCGCCATTACCGCGCTACAAGTACGATTTGGCGATTTGGGCTAACGGAGAAGAATAATGATCa                                                >  1:33265/1‑149 (MQ=255)

                                                                                                  gAAATTGCCATGACTGCCCGCTATCAATTTACTGATATCCTGTTGGGGCCTCGCGTTTTGCGTACAGAGACAACTGCGCTCACCGCCATTACCGCGCTACAAGTACGATTTGGCGATTTGGGCTAACGGAGAAGAATAATGATCAAGCTCg                                          >  2:382067/1‑151 (MQ=255)

                                                                                                   aaaTTGCCATGACTGCCCGCTATCAATTTACTGATATCCTGTGGGGGCCTCGCTTTTTGCGTACAGAGACAACTGCGCTCACCGCCATTACCGCGCTACAAGTACGATTTGGCGATTTGGGCTAACGGAGAAGAATAATGATCaa                                               <  2:592686/145‑1 (MQ=255)

                                                                                                         ccATGACTGCCCGCTATCAATTTACTGATATCCTGTGGGGCCCTCGCGTTTTGCGTACAGAGACAACTGCGCTCACCGCCATTACCGCGCTACAAGTACGATTTGGCGATTTGGGCTAACGGAGAAGAATAATGATCAAGCTCGGCATCg                                    <  1:324670/150‑1 (MQ=255)

                                                                                                            tGACTGCCCGCTATCAATTTACTGATATCCTGTTGGGGCCTCGCGTTTTGCGTACAGAGACAACTGCGCTCACCGCCATTACCGCGCTACAAGTACGATTTGGCGATTTGGGCTAACGGCGAAGAATAATGATCAAGCTCGGCATCGTGa                                 >  1:472509/1‑150 (MQ=255)

                                                                                                               cTGCCCGCTATCAATTTACTGATATCCTGTGGGGGCCTCGCGTTTTGCGTACAGAGACAACTGCGCTCACCGCCATTACCGCGCTACAAGTACGATTTGGCGATTTGGGCTAACGGAGAAGAATAATGATCAAGCTCGGCATCGTGATgg                              <  2:149982/150‑1 (MQ=255)

                                                                                                                  cccGCTATCAATTTACTGATATCCTGTGGGGGCCTCGCGTTTTGCGTAAAGAGACAACTGCGCTCACCGCCATTACCGCGCTACAAGTACGATTTGGCGATTTGGGCTAACGGAGAAGAATAATGATCAAGCTCGGCATCGTGATGGAc                            <  1:194492/149‑1 (MQ=255)

                                                                                                                       tATCAATTTACTGATATCCTGTTGGGGCCTCGCGTTTTGCGTACAGAGACAACTGCGCTCACCGCCATTACCGCGCTACAAGTACGATTTGGCGATTTGGGCTAACGGAGAAGAATAATGATCAAGCTCGGCATCGTGATGGACCCCAt                       <  2:10072/149‑1 (MQ=255)

                                                                                                                        ataaaTTTACTGATACCCTGTTGGGGCCTCGGGTTTGGCGTACAGAGACAACTGCGCTCACCGCCATTACCGCGCTACAAGTACGATTTGGCGATTTGGGCTAACGGAGAAGAATAATGATCAAGCTCGGCATCGTGATGGACCCCATc                      <  1:609509/146‑1 (MQ=255)

                                                                                                                        aTCAATTTACTGATATCCTGTTGGGGCCTCGCGTTTTGCGTACAGAGACAACTGCGCTCACCGCCATTACCGCGCTACAAGTACGATTTGGCGATTTGGGCTAACGGAGAAGAATAATGATCAAGCTCGGCATCGTGATGGACCCCATCGc                    >  2:394114/1‑151 (MQ=255)

                                                                                                                          cAATTTACTGATATCCTGTTGGGGCCTCGCGTTTTGCGTACAGAGACAACTGCGCTCACCGCCATTACCGCGCTACAAGTACGATTTGGCGATTTGGGCTAACGGAGAAGAATAATGATCAAGCTCGGCATCGTGATGGACCCCATCGCa                   >  1:410948/1‑150 (MQ=255)

                                                                                                                           attttACTTATACCCTGTTGGGGCCTGGCGTTTTGGGTACAGAGACAACTGCGCCCACCGCCATTACCGCGCTACAAGTACGATTTGGCGATTTGGGCTAACGGAGAAGAATAATGATCAAGCTCGGCATCGTGATGGACCCCATc                      <  1:261695/144‑1 (MQ=255)

                                                                                                                                 cTGATATCCTGTTGGGGCCTCGCGTTTTGCGTACAGAGACAACTGCGCTCACCGCCATTACCGCGCTACAAGTACGATTTGGCGATTTGGGCTAACGGAGAAGAATAATGATCAAGCTCGGCATCGTGATGGACCCCATCGCAaacatca            >  2:454172/1‑150 (MQ=255)

                                                                                                                                  tGATATCCTGTTGGGGCCTCGCGTTTTGCGTACAGAGACAACTGCGCTCACCGCCATTACCGCGCTACAAGTACGATTTGGCGATTTGGGCTAACGGAGAAGAATAATGATCAAGCTCGGCATCGTGATGGACCCCATCGCAaacatcaac          >  1:265906/1‑151 (MQ=255)

                                                                                                                                    atatCCTGTTGGGGCCTCGCGTTTTGCGTACAGAAACAACTGCCCTCACCGCCATTACCGCGCTACAAGTACGATTTGGCGATTTGGGCTAACGGAGAAGAATAATGATCAAGCTCGGCATCGTGATGGACCCCATCGCAaacatca            <  1:454167/147‑1 (MQ=255)

                                                                                                                                             tGGGGCCTCGCGTTTTGCGTACAGAGACAACTGCGCTCACCGCCATTACCGCGCTACAAGTACGATTTGGCGATTTGGGCTAACGGAGAAGAATAATGATCAAGCTCGGCATCGTGATGGACCCCATCGCAAACATCAACATCaagaa  >  2:416106/1‑148 (MQ=255)

ATCTTCACCCGCGCGCCAGTAACAGCATCAATACGTTGCCGTTACCGGTTGAACGCGTCCGCCTGCTGATTGGCCCGGAAGGCGGTTTATCGGCAGATGAAATTGCCATGACTGCCCGCTATCAATTTACTGATATCCTGTTGGGACCTCGCGTTTTGCGTACAGAGACAACTGCGCTCACCGCCATTACCGCGCTACAAGTACGATTTGGCGATTTGGGCTAACGGAGAAGAATAATGATCAAGCTCGGCATCGTGATGGACCCCATCGCAAACATCAACATCAAGAA  >  NC_000913/3091642‑3091930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A