Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,295,531	T→C	R46R (CGT→CGC)	yraN →	UPF0102 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,295,531	0	T	C	100.0%	109.9 / NA	32	R46R (CGT→CGC)	yraN	UPF0102 family protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (18/14); total (18/14)

CTTATCATGGCTACAGTACCAACAAGGTCAGGTAGTCCCCGTCAGTTAACCACCAAACAGACCGGCGATGCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGTGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCATTTTTGTCGAGGTACGCTATCGCCGCTCTGCGCTTTATGGCGGCGCGGCAGCCAGTGTGACCCGCAGCAAACAACACAAATTATTACAGACTGCCCGCT  >  NC_000913/3295388‑3295673

cTTATCATGGCTACAGTACCAACAAGGTCAGGTAGTCCCCGTCAGTTAACCACCAAACAGACCGGCGATGCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCgcggcggcg                                                                                                                                         >  1:323207/1‑151 (MQ=255)

  tATCATGGCTACAGTACCAACAAGGTCAGGTAGTCCCCGTCAGTTAACCACCAAACAGACCGGCGATGCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCgcggcggcg                                                                                                                                         >  2:552177/1‑149 (MQ=255)

  tATCATGGCTACAGTACCAACAAGGTCAGGTAGTCCCCGTCAGTTAACCACCAAACAGACCGGCGATGCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCga                                                                                                                                        <  1:287239/150‑1 (MQ=255)

    tCATGGCTACAGTACCAACAAGGTCAGGTAGTCCCCGTCAGTTAACCACCAAACAGACCGGCGATGCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCgcggcggcg                                                                                                                                         >  2:398206/1‑147 (MQ=255)

        ggCTACAGTACCAACAAGGTCAGGTAGTCCCCGTCAGTTAACCACCAAACAGACCGGCGATGCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAgatcgat                                                                                                                                 <  1:355132/151‑1 (MQ=255)

          cTACAGTACCAACAAGGTCAGGTAGTCCCCGTCAGTTAACCACCAAACAGACCGGCGATGCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCt                                                                                                                               >  2:222881/1‑151 (MQ=255)

           tACAGTACCAACAAGGTCAGGTAGTCCCCGTCAGTTAACCACCAAACAGACCGGCGATGCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTg                                                                                                                              >  2:278175/1‑151 (MQ=255)

            aCAGTACCAACAAGGTCAGGTAGTCCCCGTCAGTTAACCACCAAACAGACCGGCGATGCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGa                                                                                                                             <  2:419248/151‑1 (MQ=255)

              aGTACCAACAAGGTCAGGTAGTCCCCGTCAGTTAACCACCAAACAGACCGGCGATGCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGAt                                                                                                                            >  2:474430/1‑150 (MQ=255)

               gTACCAACAAGGTCAGGTAGTCCCCGTCAGTTAACCACCAAACAGACCGGCGATGCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGATa                                                                                                                           >  1:78659/1‑150 (MQ=255)

                    aacaaGGTCAGGTAGTCCCCGTCAGTTAACCACCAAACAGACCGGCGATGCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGt                                                                                                                     <  2:547508/151‑1 (MQ=255)

                      caaGGTCAGGTAGTCCCCGTCAGTTAACCACCAAACAGACCGGCGATGCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTg                                                                                                                    >  2:201660/1‑150 (MQ=255)

                                      ccGTCAGTTAACCACCAAACAGACCGGCGATGCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCAtt                                                                                                   >  1:438215/1‑151 (MQ=255)

                                                             ccGGCGATGCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCATTTTTGTCGAGGTACGCTATcgccg                                                                            <  1:147758/151‑1 (MQ=255)

                                                              cGGCGATGCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCATTTTTGTCGAGGTACGCTATcgccgc                                                                           >  1:68489/1‑151 (MQ=255)

                                                              cGGCGATGCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCATTTTTGTCGAGGTACGCTATcgccgc                                                                           >  1:184558/1‑151 (MQ=255)

                                                                gCGATGCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCATTTTTGTCGAGGTACGCTATCGCCGctc                                                                         >  2:85350/1‑151 (MQ=255)

                                                                  gATGCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCATTTTTGTCGAGGTACGCTATCGCCGCTCTg                                                                       <  2:153870/151‑1 (MQ=255)

                                                                     gCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCATTTTTGTCGAGGTACGCTAt                                                                                 <  2:378276/138‑1 (MQ=255)

                                                                     gCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCATTTTTGTCGAGGTACGCTAt                                                                                 >  1:378272/1‑138 (MQ=255)

                                                                     gCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCATTTTTGTCGAGGTACGCTATCGCCGCTCTgcgc                                                                    >  1:315735/1‑151 (MQ=255)

                                                                            aaGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCATTTTTGTCGAGGTACGCTATCGCCGCTCTGCGCTTTATgg                                                             <  1:648216/151‑1 (MQ=255)

                                                                               cacaAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCATTTTTGTCGAGGTACGCTATCGCCGCTCTGCGCTTTATggcgg                                                          >  2:654970/1‑151 (MQ=255)

                                                                                    gcgcGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCATTTTTGTCGAGGTACGCTATCGCCGCTCTGCGCTTTATGgcggcgcgg                                                     <  1:278175/151‑1 (MQ=255)

                                                                                      gcgTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCATTTTTGTCGAGGTACGCTATCGCCGCTCTGCGCTTTATGGCGGCGCGGCa                                                   <  1:153848/151‑1 (MQ=255)

                                                                                       cgTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCATTTTTGTCGAGGTACGCTATCGCCGCTCTGCGCTTTATGgcggcgccgc                                                    >  1:326041/1‑149 (MQ=255)

                                                                                                   gAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTCAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCAtttt                                                                                                 <  1:429743/92‑1 (MQ=255)

                                                                                                   gAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTCAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCAtttt                                                                                                 >  2:429748/1‑92 (MQ=255)

                                                                                                        cAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCATTTTTGTCGAGGTACGCTATCGCCGCTCTGCGCTTTATGGCGGCGCGGCAGCCAGTGTGACCCgcagc                                 >  2:449651/1‑151 (MQ=255)

                                                                                                           aGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCATTTTTGTCGAGGTACGCTATCGCCGCTCTGCGCTTTATGGCGGCGCGGCAGCCAGTGTGACCCGCAGCaaa                              <  2:313299/151‑1 (MQ=255)

                                                                                                                tGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCATTTTTGTCGAGGTACGCTATCGCCGCTCTGCGCTTTATGGCGGCGCGGCAGCCAGTGTGACCCGCAGCAaacaac                          >  1:147137/1‑150 (MQ=255)

                                                                                                                           gccgcTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCATTTTTGTCGAGGTACGCTATCGCCGCTCTGCGCTTTATGGCGGCGCGGCAGCCAGTGTGACCCGCAGCAAACAACACAAAttatta               >  1:173546/1‑150 (MQ=255)

                                                                                                                                    gTGAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCATTTTTGTCGAGGTACGCTATCGCCGCTCTGCGCTTTATGGCGGCGCGGCAGCCAGTGTGACCCGCAGCAAACAACACAAATTATTACAGACTGccc     <  2:288566/151‑1 (MQ=255)

                                                                                                                                     tGAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCATTTTTGTCGAGGTACGCTATCGCCGCTCTGCGCTTTATGGCGGCGCGGGAGCCAGTGTGACCCGCAGCAAACAACACAAATTATTACAGACTGCCCg    >  1:360290/1‑151 (MQ=255)

                                                                                                                                       aaCGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCATTTTTGTCGAGGTACGCTATCGCCGCTCTGCGCTTTATGGCGGCGCGGCAGCCAGTGTGACCCGCAGCAAACAACACAAATTATTACAGACTGCCCGCt  <  1:222880/151‑1 (MQ=255)

CTTATCATGGCTACAGTACCAACAAGGTCAGGTAGTCCCCGTCAGTTAACCACCAAACAGACCGGCGATGCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGTGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCATTTTTGTCGAGGTACGCTATCGCCGCTCTGCGCTTTATGGCGGCGCGGCAGCCAGTGTGACCCGCAGCAAACAACACAAATTATTACAGACTGCCCGCT  >  NC_000913/3295388‑3295673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A