Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,311,285	T→C	intergenic (‑117/+130)	pnp ← / ← rpsO	polynucleotide phosphorylase/polyadenylase/30S ribosomal subunit protein S15

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,311,285	0	T	C	100.0%	145.2 / NA	38	intergenic (‑117/+130)	pnp/rpsO	polynucleotide phosphorylase/polyadenylase/30S ribosomal subunit protein S15
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (19/19); total (19/19)

ACTGGAATTTACGAACGATCGGATTAAGCAATGTAATATCCTTTCTCTTTCTTAGACAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCTCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGAATTAGCGACGCAGACCC  >  NC_000913/3311138‑3311430

aCTGGAATTTACGAACGATCGGATTAAGCAATGTAATATCCTTTCTCTTTCTTAGACAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCccct                                                                                                                                                 <  2:184796/150‑1 (MQ=255)

    gAATTTACGAACGATCGGATTAAGCAATGTAATATCCTTTCTCTTTCTTAGACAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTa                                                                                                                                            <  1:661480/151‑1 (MQ=255)

     aaTTTACGAACGATCGGATTAAGCAATGTAATATCCTTTCTCTTTCTTAGACAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAg                                                                                                                                           <  1:508178/151‑1 (MQ=255)

       tttACGAACGATCGGATTAAGCAATGTAATATCCTTTCTCTTTCTTAGACAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGcc                                                                                                                                         >  2:7534/1‑151 (MQ=255)

                    ggATTAAGCAATGTAATATCCTTTCTCTTTCTTAGACAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTg                                                                                                                            <  2:111229/151‑1 (MQ=255)

                        tAAGCAATGTAATATCCTTTCTCTTTCTTAGACAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCaa                                                                                                                         <  1:383151/150‑1 (MQ=255)

                         aaGCAATGTAATATCCTTTCTCTTTCTTAGACAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACg                                                                                                                       <  1:613936/151‑1 (MQ=255)

                          aGCAATGTAATATCCTTTCTCTTTCTTAGACAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACgg                                                                                                                      <  2:598175/151‑1 (MQ=255)

                            cAATGTAATATCCTTTCTCTTTCTTAGACAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGaa                                                                                                                    <  1:544491/151‑1 (MQ=255)

                              aTGTAATATCCTTTCTCTTTCTTAGACAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGa                                                                                                                  <  1:500500/151‑1 (MQ=255)

                               tGTAATATCCTTTCTCTTTCTTAGACAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGa                                                                                                                     >  2:572095/1‑147 (MQ=255)

                               tGTAATATCCTTTCTCTTTCTTAGACAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCCCATCCTCGCGACTAATGACACCCCTAACCCAGCACTATGTGGGGAAAGCCCCTCAGTAGCCGCGCGAACCACTGCAACGGAAg                                                                                                                   <  1:10331/149‑1 (MQ=255)

                                gTAATATCCTTTCTCTTTCTTAGACAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGAtc                                                                                                                <  1:342217/151‑1 (MQ=255)

                                  aaTATCCTTTCTCTTTCTTAGACAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGAtcat                                                                                                              >  1:527853/1‑151 (MQ=255)

                                    tatCCTTTCTCTTTCTTAGACAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGAtcattc                                                                                                            >  2:378330/1‑151 (MQ=255)

                                      tCCTTTCTCTTTCTTAGACAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGAtcattc                                                                                                            >  2:243388/1‑149 (MQ=255)

                                         tttctctttctTAGACAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGc                                                                                                       >  1:141910/1‑151 (MQ=255)

                                           tctctttctTAGACAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGca                                                                                                      <  2:87531/150‑1 (MQ=255)

                                                ttcttAGACAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAAt                                                                                                 >  2:226017/1‑150 (MQ=255)

                                                   ttAGACAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACAt                                                                                             >  2:143116/1‑151 (MQ=255)

                                                       aCAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGt                                                                                         <  1:584904/151‑1 (MQ=255)

                                                        cAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGtt                                                                                        <  1:34192/151‑1 (MQ=255)

                                                              cTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGt                                                                                  >  2:357357/1‑151 (MQ=255)

                                                                               aaTACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGc                                                                 <  2:636042/151‑1 (MQ=255)

                                                                                   cccGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTg                                                              >  1:69447/1‑150 (MQ=255)

                                                                                          ttctGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAgg                                                      >  1:313014/1‑151 (MQ=255)

                                                                                           tctGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGaaaa                                                          <  2:535023/146‑1 (MQ=255)

                                                                                                gcATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAGGGGCCa                                                 >  1:657337/1‑150 (MQ=255)

                                                                                                 cATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAGGGGCCACt                                               >  1:66152/1‑151 (MQ=255)

                                                                                                        gcgACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAGGGGCCACTCAGGccc                                        >  1:273484/1‑151 (MQ=255)

                                                                                                           aCTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAGGGGCCACTCAGGCCCCCt                                     <  2:66737/151‑1 (MQ=255)

                                                                                                              aaTGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAGGGGCCACTCAGGCCCCCtttt                                  <  1:288294/151‑1 (MQ=255)

                                                                                                                tGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAGGGGCCACTCAGGCCCCCTTTTCt                                >  1:283118/1‑151 (MQ=255)

                                                                                                                             ccAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGa                   <  2:624028/151‑1 (MQ=255)

                                                                                                                               aGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGAAt                 >  2:92937/1‑151 (MQ=255)

                                                                                                                                 ctctATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGAAtt                >  2:565537/1‑150 (MQ=255)

                                                                                                                                  tctATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGAAtt                >  1:472174/1‑149 (MQ=255)

                                                                                                                                           gTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGAATTAGCGACGCAGa     <  2:344417/151‑1 (MQ=255)

                                                                                                                                              aaGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGAATTAGCGACGCAGAccc  >  2:543054/1‑151 (MQ=255)

ACTGGAATTTACGAACGATCGGATTAAGCAATGTAATATCCTTTCTCTTTCTTAGACAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCTCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGAATTAGCGACGCAGACCC  >  NC_000913/3311138‑3311430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A