Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F50 I2 R1 193 39.9 1341687 91.8% 1231668 149.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,575,617 G→A A183T (GCA→ACA)  yhgN → UPF0056 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,575,6170GA100.0% 190.2 / NA 50A183T (GCA→ACA) yhgNUPF0056 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/25);  total (25/25)

CAGATGGGGCATCTGGTGATTGCTCTGCTGCTGGCCTGGGGCGGCACCTTTGTCATCCTGCTACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGGCAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACTACCGGGCGAACAAACTTAACTCAACAGCTGGAACGCAATCATCCCAACGATGGCAC  >  NC_000913/3575470‑3575761
                                                                                                                                                   |                                                                                                                                                
cAGATGGGGCATCTGGTGATTGCTCTGCTGCTGGCCTGGGGCGGCACCTTTGTCATCCTGCTACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACa                                                                                                                                                <  1:206183/150‑1 (MQ=255)
   aTGGGGCATCTGGTGATTGCTCTGCTGCTGGCCTGGGGCGGCACCTTTGTCATCCTGCTACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAAccc                                                                                                                                            <  2:585401/151‑1 (MQ=255)
     ggggCATCTGGTGATTGCTCTGCTGCTGGCCTGGGGCGGCACCTTTGTCATCCTGCTACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCa                                                                                                                                           <  2:29937/150‑1 (MQ=255)
     ggggCATCTGGTGATTGCTCTGCTGCTGGCCTGGGGCGGCACCTTTGTCATCCTGCTACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAg                                                                                                                                          <  1:165077/151‑1 (MQ=255)
      gggCATCTGGTGATTGCTCTGCTGCTGGCCTGGGGCGGCACCTTTGTCATCCTGCTACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAAccc                                                                                                                                            >  2:173624/1‑148 (MQ=255)
      gggCATCTGGTGATTGCTCTGCTGCTGGCCTGGGGCGGCACCTTTGTCATCCTGCTACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAAccc                                                                                                                                            >  2:670271/1‑148 (MQ=255)
      gggCATCTGGTGATTGCTCTGCTGCTGGCCTGGGGCGGCACCTTTGTCATCCTGCTACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAAccc                                                                                                                                            >  2:59488/1‑148 (MQ=255)
         cATCTGGTGATTGCTCTGCTGCTGGCCTGGGGCGGCACCTTTGTCATCCTGCTACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGAt                                                                                                                                        <  2:442167/149‑1 (MQ=255)
          aTCTGGTGATTGCTCTGCTGCTGGCCTGGGGCGGCACCTTTGTCATCCTGCTACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGtt                                                                                                                                     >  1:565639/1‑151 (MQ=255)
            cTGGTGATTGCTCTGCTGCTGGCCTGGGGCGGCACCTTTGTCATCCTGCTACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTcc                                                                                                                                   >  1:357014/1‑151 (MQ=255)
              ggTGATTGCTCTGCTGCTGGCCTGGGGCGGCACCTTTGTCATCCTGCTACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTcc                                                                                                                                   <  1:460340/149‑1 (MQ=255)
              ggTGATTGCTCTGCTGCTGGCCTGGGGCGGCACCTTTGTCATCCTGCTACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTc                                                                                                                                 >  2:84412/1‑151 (MQ=255)
                tGATTGCTCTGCTGCTGGCCTGGGGCGGCACCTTTGTCATCCTGCTACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCg                                                                                                                                >  1:526145/1‑150 (MQ=255)
                          gctgctgGCCTGGGGCGGCACCTTTGTCATCCTGCTACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGa                                                                                                                     <  1:135064/151‑1 (MQ=255)
                           ctgctgGCCTGGGGCGGCACCTTTGTCATCCTGCTACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGa                                                                                                                     >  2:425495/1‑150 (MQ=255)
                             gctgGCCTGGGGCGGCACCTTTGTCATCCTGCTACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAAt                                                                                                                   <  1:220562/150‑1 (MQ=255)
                              ctgGCCTGGGGCGGCACCTTTGTCATCCTGCTACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAAtgt                                                                                                                 >  2:95816/1‑151 (MQ=255)
                                     ggggCGGCACCTTTGTCATCCTGCTACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATg                                                                                                            <  1:610420/149‑1 (MQ=255)
                                      gggCGGCACCTTTGTCATCCTGCTACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAg                                                                                                         <  2:597198/151‑1 (MQ=255)
                                                           gCTACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGttttt                                                                                     <  1:460113/150‑1 (MQ=255)
                                                             tACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCg                                                                                   >  2:664210/1‑150 (MQ=255)
                                                              aCAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCg                                                                                   <  1:578880/149‑1 (MQ=255)
                                                                     tCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGaa                                                                            <  1:551049/149‑1 (MQ=255)
                                                                      cGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAAccc                                                                         <  1:282706/151‑1 (MQ=255)
                                                                        cTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCgg                                                                       >  2:419277/1‑151 (MQ=255)
                                                                               tACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTc                                                                >  2:352623/1‑151 (MQ=255)
                                                                                aCGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTca                                                               <  1:59489/151‑1 (MQ=255)
                                                                                aCGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTc                                                                >  2:362217/1‑150 (MQ=255)
                                                                                    ctgctgGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAAc                                                           >  2:27347/1‑151 (MQ=255)
                                                                                       ctgGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACTAc                                                        >  1:264156/1‑151 (MQ=255)
                                                                                         gggCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACTACCg                                                      <  1:664199/151‑1 (MQ=255)
                                                                                           gCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACTACCggg                                                    >  2:363324/1‑151 (MQ=255)
                                                                                            cGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACTACCGGGc                                                   <  1:425490/151‑1 (MQ=255)
                                                                                             gagaAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACTACCGGGCg                                                  <  1:629014/151‑1 (MQ=255)
                                                                                                 aaGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACGACCGGGCGAACa                                              <  2:24372/151‑1 (MQ=255)
                                                                                                     ggTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACTACCGGGCGAACAAACt                                          <  2:163537/151‑1 (MQ=255)
                                                                                                     ggTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACTACCGGGCGAACAAACt                                          <  1:73761/151‑1 (MQ=255)
                                                                                                      gTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACTACCGGGCGAACAAACtt                                         <  2:280181/151‑1 (MQ=255)
                                                                                                              aCTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACTACCGGGCGAACAAACTTAACTCAAc                                 >  2:331502/1‑151 (MQ=255)
                                                                                                                   aaCGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACTACCGGGCGAACAAACTTAACTCAACAGCTg                            >  2:195633/1‑151 (MQ=255)
                                                                                                                     cGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACTACCGGGCGAACAAACTTAACTCAACAGCTgg                           >  1:349684/1‑150 (MQ=255)
                                                                                                                       ccTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACTACCGGGCGAACAAACTTAACTCAACAGCTGGAAc                        <  2:575232/151‑1 (MQ=255)
                                                                                                                       ccTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACTACCGGGCGAACAAACTTAACTCAACAGCTGGAAc                        <  1:37722/151‑1 (MQ=255)
                                                                                                                        cTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACTACCGGGCGAACAAACTTAACTCAACAGCTGGAACg                       >  2:587453/1‑151 (MQ=255)
                                                                                                                             gggATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACTACCGGGCGAACAAACTTAACTCAACAGCTGGAACGCAatc                  >  2:458656/1‑151 (MQ=255)
                                                                                                                               gattgattCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACTACCGGGCGAACAAACTTAACTCAACAGCTGGAACGCAatcat                >  1:550578/1‑151 (MQ=255)
                                                                                                                                      tCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACTACCGGGCGAACAAACTTAACTCAACAGCTGGAACGCAATCATCCCAAc          <  1:60870/150‑1 (MQ=255)
                                                                                                                                       cTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACTACCGGGCGAACAAACTTAACTCAACAGCTGGAACGCAATCATCCCAACg         >  2:156140/1‑150 (MQ=255)
                                                                                                                                            gatgatGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACTACCGGGCGAACAAACTTAACTCAACAGCTGGAACGCAATCATCCCAACGATGGc    >  1:114121/1‑150 (MQ=255)
                                                                                                                                             atgatgACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACTACCGGGCGAACAAACTTAACTCAACAGCTGGAACGCAATCATCCCAACGATGGCAc  >  2:464387/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CAGATGGGGCATCTGGTGATTGCTCTGCTGCTGGCCTGGGGCGGCACCTTTGTCATCCTGCTACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGGCAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACTACCGGGCGAACAAACTTAACTCAACAGCTGGAACGCAATCATCCCAACGATGGCAC  >  NC_000913/3575470‑3575761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A